Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase

Gene

Gne

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. Required for normal sialylation in hematopoietic cells (By similarity). Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells. Plays an essential role in early development.By similarity1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + H2O = N-acetyl-D-mannosamine + UDP.
ATP + N-acyl-D-mannosamine = ADP + N-acyl-D-mannosamine 6-phosphate.

Enzyme regulationi

Allosterically regulated; feedback inhibited by cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac), the end product of neuraminic acid biosynthesis. Activity is dependent on oligomerization. The monomer is inactive, whereas the dimer catalyzes only the phosphorylation of N-acetylmannosamine; the hexamer is fully active for both enzyme activities (By similarity). Up-regulated after PKC-dependent phosphorylation.By similarity

Pathwayi: N-acetylneuraminate biosynthesis

This protein is involved in the pathway N-acetylneuraminate biosynthesis, which is part of Amino-sugar metabolism.
View all proteins of this organism that are known to be involved in the pathway N-acetylneuraminate biosynthesis and in Amino-sugar metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei477SubstrateBy similarity1
Binding sitei489SubstrateBy similarity1
Active sitei517By similarity1
Binding sitei517SubstrateBy similarity1
Binding sitei566SubstrateBy similarity1
Metal bindingi569ZincBy similarity1
Binding sitei569SubstrateBy similarity1
Metal bindingi579ZincBy similarity1
Metal bindingi581ZincBy similarity1
Metal bindingi586ZincBy similarity1
Binding sitei588SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi411 – 420ATPBy similarity10
Nucleotide bindingi543 – 552ATPBy similarity10

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Hydrolase, Kinase, Multifunctional enzyme, Transferase
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.60 3474
3.2.1.183 3474
ReactomeiR-MMU-4085001 Sialic acid metabolism
UniPathwayiUPA00630

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
Alternative name(s):
UDP-GlcNAc-2-epimerase/ManAc kinase
Including the following 2 domains:
UDP-N-acetylglucosamine 2-epimerase (hydrolyzing) (EC:3.2.1.183)
Alternative name(s):
UDP-GlcNAc-2-epimerase
Uridine diphosphate-N-acetylglucosamine-2-epimerase
N-acetylmannosamine kinase (EC:2.7.1.60)
Alternative name(s):
ManAc kinase
Gene namesi
Name:Gne
Synonyms:Glcne, Uae1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1354951 Gne

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000957171 – 722Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinaseAdd BLAST722

Post-translational modificationi

Phosphorylated by PKC.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ91WG8
MaxQBiQ91WG8
PaxDbiQ91WG8
PRIDEiQ91WG8

PTM databases

iPTMnetiQ91WG8
PhosphoSitePlusiQ91WG8
SwissPalmiQ91WG8

Expressioni

Tissue specificityi

Widely expressed. Highest expression in liver. Also found at high levels in lung, brain and kidney.1 Publication

Developmental stagei

In the embryo, expressed at day E7, E11 and E15.1 Publication

Gene expression databases

BgeeiENSMUSG00000028479
CleanExiMM_GNE
ExpressionAtlasiQ91WG8 baseline and differential
GenevisibleiQ91WG8 MM

Interactioni

Subunit structurei

Homodimer and homohexamer.By similarity

Protein-protein interaction databases

IntActiQ91WG8, 1 interactor
MINTiQ91WG8
STRINGi10090.ENSMUSP00000030201

Structurei

3D structure databases

ProteinModelPortaliQ91WG8
SMRiQ91WG8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – ?UDP-N-acetylglucosamine 2-epimerase
Regioni406 – 722N-acetylmannosamine kinaseBy similarityAdd BLAST317

Sequence similaritiesi

In the N-terminal section; belongs to the UDP-N-acetylglucosamine 2-epimerase family.Curated
In the C-terminal section; belongs to the ROK (NagC/XylR) family.Curated

Phylogenomic databases

eggNOGiENOG410IE3W Eukaryota
COG0381 LUCA
COG1940 LUCA
GeneTreeiENSGT00390000017246
HOGENOMiHOG000008254
HOVERGENiHBG051733
InParanoidiQ91WG8
KOiK12409

Family and domain databases

CDDicd03786 GT1_UDP-GlcNAc_2-Epimerase, 1 hit
InterProiView protein in InterPro
IPR000600 ROK
IPR020004 UDP-GlcNAc_Epase
IPR003331 UDP_GlcNAc_Epimerase_2_dom
PANTHERiPTHR18964 PTHR18964, 1 hit
PfamiView protein in Pfam
PF02350 Epimerase_2, 1 hit
PF00480 ROK, 1 hit
TIGRFAMsiTIGR03568 NeuC_NnaA, 1 hit

Sequencei

Sequence statusi: Complete.

Q91WG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKNGNNRKL RVCVATCNRA DYSKLAPIMF GIKTEPAFFE LDVVVLGSHL
60 70 80 90 100
IDDYGNTYRM IEQDDFDINT RLHTIVRGED EAAMVESVGL ALVKLPDVLN
110 120 130 140 150
RLKPDIMIVH GDRFDALALA TSAALMNIRI LHIEGGEVSG TIDDSIRHAI
160 170 180 190 200
TKLAHYHVCC TRSAEQHLIS MCEDHDRILL AGCPSYDKLL SAKNKDYMSI
210 220 230 240 250
IRMWLGDDVK CKDYIVALQH PVTTDIKHSI KMFELTLDAL ISFNKRTLVL
260 270 280 290 300
FPNIDAGSKE MVRVMRKKGI EHHPNFRAVK HVPFDQFIQL VAHAGCMIGN
310 320 330 340 350
SSCGVREVGA FGTPVINLGT RQIGRETGEN VLHVRDADTQ DKILQALHLQ
360 370 380 390 400
FGKQYPCSKI YGDGNAVPRI LKFLKSIDLQ EPLQKKFCFP PVKENISQDI
410 420 430 440 450
DHILETLSAL AVDLGGTNLR VAIVSMKGEI VKKYTQFNPK TYEERISLIL
460 470 480 490 500
QMCVEAAAEA VKLNCRILGV GISTGGRVNP QEGVVLHSTK LIQEWNSVDL
510 520 530 540 550
RTPLSDTLHL PVWVDNDGNC AAMAERKFGQ GKGQENFVTL ITGTGIGGGI
560 570 580 590 600
IHQHELIHGS SFCAAELGHL VVSLDGPDCS CGSHGCIEAY ASGMALQREA
610 620 630 640 650
KKLHDEDLLL VEGMSVPKDE AVGALHLIQA AKLGNVKAQS ILRTAGTALG
660 670 680 690 700
LGVVNILHTM NPSLVILSGV LASHYIHIVK DVIRQQALSS VQDVDVVVSD
710 720
LVDPALLGAA SMVLDYTTRR IH
Length:722
Mass (Da):79,199
Last modified:December 1, 2001 - v1
Checksum:i0DFDD681BFD17984
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti566 – 567EL → DV in CAB36908 (PubMed:10103025).Curated2
Sequence conflicti623G → V in CAB36908 (PubMed:10103025).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132236 mRNA Translation: CAB36908.1
BC015277 mRNA Translation: AAH15277.1
BC051254 mRNA Translation: AAH51254.1
AK033507 mRNA Translation: BAC28328.1
AK033691 mRNA Translation: BAC28432.1
CCDSiCCDS51170.1
RefSeqiNP_001177343.1, NM_001190414.1
NP_056643.3, NM_015828.3
UniGeneiMm.256718

Genome annotation databases

EnsembliENSMUST00000102936; ENSMUSP00000100000; ENSMUSG00000028479
GeneIDi50798
KEGGimmu:50798
UCSCiuc008srm.2 mouse

Similar proteinsi

Entry informationi

Entry nameiGLCNE_MOUSE
AccessioniPrimary (citable) accession number: Q91WG8
Secondary accession number(s): Q8CC83, Q8CCB0, Q9Z0P6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: December 1, 2001
Last modified: June 20, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health