UniProtKB - Q91WA6 (SHRPN_MOUSE)
Sharpin
Sharpin
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 342 – 371 | RanBP2-typePROSITE-ProRule annotationAdd BLAST | 30 |
GO - Molecular functioni
- identical protein binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- polyubiquitin modification-dependent protein binding Source: UniProtKB
- protein-containing complex binding Source: MGI
- ubiquitin binding Source: GO_Central
- ubiquitin-protein transferase activity Source: GO_Central
GO - Biological processi
- apoptotic nuclear changes Source: MGI
- brain development Source: Ensembl
- epidermis development Source: MGI
- keratinization Source: MGI
- mitochondrion organization Source: MGI
- negative regulation of inflammatory response Source: UniProtKB
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
- protein linear polyubiquitination Source: UniProtKB
- regulation of CD40 signaling pathway Source: UniProtKB
- regulation of tumor necrosis factor-mediated signaling pathway Source: UniProtKB
Keywordsi
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-5357905, Regulation of TNFR1 signaling R-MMU-5357956, TNFR1-induced NFkappaB signaling pathway |
Names & Taxonomyi
Protein namesi | Recommended name: SharpinAlternative name(s): Shank-associated RH domain-interacting protein Shank-interacting protein-like 1 Short name: mSIPL1 |
Gene namesi | Name:Sharpin Synonyms:Cpdm, Sipl1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1913331, Sharpin |
Subcellular locationi
Cytosol
- cytosol Source: UniProtKB
Other locations
- dendrite Source: MGI
- LUBAC complex Source: UniProtKB
- postsynaptic density Source: MGI
- ubiquitin ligase complex Source: GO_Central
Keywords - Cellular componenti
Cell junction, Cytoplasm, SynapsePathology & Biotechi
Involvement in diseasei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000280635 | 1 – 380 | SharpinAdd BLAST | 380 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 163 | PhosphoserineBy similarity | 1 | |
Modified residuei | 307 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q91WA6 |
PaxDbi | Q91WA6 |
PeptideAtlasi | Q91WA6 |
PRIDEi | Q91WA6 |
PTM databases
iPTMneti | Q91WA6 |
PhosphoSitePlusi | Q91WA6 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000022552, Expressed in external carotid artery and 301 other tissues |
ExpressionAtlasi | Q91WA6, baseline and differential |
Genevisiblei | Q91WA6, MM |
Interactioni
Subunit structurei
Monomer and homodimer (By similarity).
Component of the LUBAC complex (linear ubiquitin chain assembly complex) which consists of SHARPIN, RBCK1 and RNF31 (By similarity). LUBAC has a MW of approximately 600 kDa suggesting a heteromultimeric assembly of its subunits (By similarity). Associates with the TNF-R1 signaling complex (TNF-RSC) in a stimulation-dependent manner (By similarity).
Interacts with EYA1, EYA2, SHANK1 and SHANK3 (via ANK repeats) (PubMed:20956555).
By similarity1 PublicationBinary interactionsi
Hide detailsQ91WA6
With | #Exp. | IntAct |
---|---|---|
Eya1 [P97767] | 4 | EBI-646097,EBI-1368503 |
Eya2 [O08575] | 2 | EBI-646097,EBI-986503 |
Rnf31 [Q924T7] | 10 | EBI-646097,EBI-647680 |
GO - Molecular functioni
- identical protein binding Source: MGI
- polyubiquitin modification-dependent protein binding Source: UniProtKB
- ubiquitin binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 222977, 25 interactors |
DIPi | DIP-49461N |
IntActi | Q91WA6, 6 interactors |
MINTi | Q91WA6 |
STRINGi | 10090.ENSMUSP00000023211 |
Miscellaneous databases
RNActi | Q91WA6, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q91WA6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 216 – 285 | Ubiquitin-likeAdd BLAST | 70 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 177 | Self-associationBy similarityAdd BLAST | 177 | |
Regioni | 172 – 305 | Interaction with SHANK1By similarityAdd BLAST | 134 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 342 – 371 | RanBP2-typePROSITE-ProRule annotationAdd BLAST | 30 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG1815, Eukaryota |
GeneTreei | ENSGT00940000161574 |
HOGENOMi | CLU_014998_0_1_1 |
InParanoidi | Q91WA6 |
OMAi | YTIRGPS |
OrthoDBi | 1086223at2759 |
PhylomeDBi | Q91WA6 |
TreeFami | TF323486 |
Family and domain databases
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR011993, PH-like_dom_sf IPR026261, RBCK1/SHARPIN IPR031912, Sharpin_PH IPR029071, Ubiquitin-like_domsf IPR001876, Znf_RanBP2 IPR036443, Znf_RanBP2_sf |
PANTHERi | PTHR22770:SF43, PTHR22770:SF43, 1 hit |
Pfami | View protein in Pfam PF16764, Sharpin_PH, 1 hit |
SMARTi | View protein in SMART SM00547, ZnF_RBZ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit SSF90209, SSF90209, 1 hit |
PROSITEi | View protein in PROSITE PS01358, ZF_RANBP2_1, 1 hit PS50199, ZF_RANBP2_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSPPAGGAAV AADPASPVVL LAVHAAVRPL GAGQDAEAQP RKLQLIADPE
60 70 80 90 100
RPGRFRLGLL GTEPGAVSLE WPLEAICYTV RGPNQHELQP PPGGPGTFSV
110 120 130 140 150
HFLDPEEAQQ WAALVRDATA EGQNGSGSPA PAPAPAMCPI SPPCSSMAQI
160 170 180 190 200
PKATQPEVDL PQSSGNFKKE ELATRLSQAI AGGDEKAAAQ VAAVLAQHHV
210 220 230 240 250
ALNVQLMEAW FPPGPIRLQV TVEDATSVLS SSSSAHVSLK IHPHCSIAAL
260 270 280 290 300
QDQVFSEFGF PPAVQRWVIG RCLCMPERSL ASYGVSQDGD PAFLYLLSAP
310 320 330 340 350
REVSGQSLQN SKMDRKLGLF PQSLGLPHDL QPSSSSLPSP SQPGWSCPSC
360 370 380
TFINASNRPG CEMCSTQRPC AWDPLAAAST
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF6UHH8 | F6UHH8_MOUSE | Sharpin | Sharpin | 83 | Annotation score: | ||
E0CZH2 | E0CZH2_MOUSE | Sharpin | Sharpin | 157 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 131 | P → Q in BAC53796 (PubMed:12753155).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_023839 | 306 – 380 | Missing in isoform 2. 1 PublicationAdd BLAST | 75 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB052763 mRNA Translation: BAC53796.1 BC016203 mRNA Translation: AAH16203.1 BC055758 mRNA Translation: AAH55758.1 |
CCDSi | CCDS37121.1 [Q91WA6-1] |
RefSeqi | NP_079616.2, NM_025340.2 [Q91WA6-1] |
Genome annotation databases
Ensembli | ENSMUST00000023211; ENSMUSP00000023211; ENSMUSG00000022552 [Q91WA6-1] |
GeneIDi | 106025 |
KEGGi | mmu:106025 |
UCSCi | uc007wju.2, mouse [Q91WA6-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB052763 mRNA Translation: BAC53796.1 BC016203 mRNA Translation: AAH16203.1 BC055758 mRNA Translation: AAH55758.1 |
CCDSi | CCDS37121.1 [Q91WA6-1] |
RefSeqi | NP_079616.2, NM_025340.2 [Q91WA6-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5Y3T | X-ray | 2.40 | C | 163-341 | [»] | |
SMRi | Q91WA6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 222977, 25 interactors |
DIPi | DIP-49461N |
IntActi | Q91WA6, 6 interactors |
MINTi | Q91WA6 |
STRINGi | 10090.ENSMUSP00000023211 |
PTM databases
iPTMneti | Q91WA6 |
PhosphoSitePlusi | Q91WA6 |
Proteomic databases
EPDi | Q91WA6 |
PaxDbi | Q91WA6 |
PeptideAtlasi | Q91WA6 |
PRIDEi | Q91WA6 |
Protocols and materials databases
Antibodypediai | 7700, 239 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000023211; ENSMUSP00000023211; ENSMUSG00000022552 [Q91WA6-1] |
GeneIDi | 106025 |
KEGGi | mmu:106025 |
UCSCi | uc007wju.2, mouse [Q91WA6-1] |
Organism-specific databases
CTDi | 81858 |
MGIi | MGI:1913331, Sharpin |
Phylogenomic databases
eggNOGi | KOG1815, Eukaryota |
GeneTreei | ENSGT00940000161574 |
HOGENOMi | CLU_014998_0_1_1 |
InParanoidi | Q91WA6 |
OMAi | YTIRGPS |
OrthoDBi | 1086223at2759 |
PhylomeDBi | Q91WA6 |
TreeFami | TF323486 |
Enzyme and pathway databases
Reactomei | R-MMU-5357905, Regulation of TNFR1 signaling R-MMU-5357956, TNFR1-induced NFkappaB signaling pathway |
Miscellaneous databases
BioGRID-ORCSi | 106025, 6 hits in 21 CRISPR screens |
ChiTaRSi | Sharpin, mouse |
PROi | PR:Q91WA6 |
RNActi | Q91WA6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022552, Expressed in external carotid artery and 301 other tissues |
ExpressionAtlasi | Q91WA6, baseline and differential |
Genevisiblei | Q91WA6, MM |
Family and domain databases
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR011993, PH-like_dom_sf IPR026261, RBCK1/SHARPIN IPR031912, Sharpin_PH IPR029071, Ubiquitin-like_domsf IPR001876, Znf_RanBP2 IPR036443, Znf_RanBP2_sf |
PANTHERi | PTHR22770:SF43, PTHR22770:SF43, 1 hit |
Pfami | View protein in Pfam PF16764, Sharpin_PH, 1 hit |
SMARTi | View protein in SMART SM00547, ZnF_RBZ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit SSF90209, SSF90209, 1 hit |
PROSITEi | View protein in PROSITE PS01358, ZF_RANBP2_1, 1 hit PS50199, ZF_RANBP2_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SHRPN_MOUSE | |
Accessioni | Q91WA6Primary (citable) accession number: Q91WA6 Secondary accession number(s): Q7TNT3, Q8CHL3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 2007 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 10, 2021 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references