UniProtKB - Q91WA1 (TIPIN_MOUSE)
Protein
TIMELESS-interacting protein
Gene
Tipin
Organism
Mus musculus (Mouse)
Status
Functioni
Plays an important role in the control of DNA replication and the maintenance of replication fork stability. Important for cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. Forms a complex with TIMELESS and this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress.1 Publication
GO - Molecular functioni
- DNA binding Source: GO_Central
GO - Biological processi
- cell cycle phase transition Source: BHF-UCL
- cell division Source: UniProtKB-KW
- DNA damage checkpoint Source: MGI
- DNA replication checkpoint Source: UniProtKB
- intra-S DNA damage checkpoint Source: UniProtKB
- positive regulation of cell population proliferation Source: UniProtKB
- replication fork arrest Source: GO_Central
- replication fork protection Source: GO_Central
- response to UV Source: MGI
Keywordsi
Biological process | Cell cycle, Cell division, DNA damage, Mitosis |
Enzyme and pathway databases
Reactomei | R-MMU-5693607, Processing of DNA double-strand break ends |
Names & Taxonomyi
Protein namesi | Recommended name: TIMELESS-interacting protein |
Gene namesi | Name:Tipin |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1921571, Tipin |
Subcellular locationi
Nucleus
- nuclear chromatin Source: UniProtKB
- nucleus Source: MGI
- replication fork protection complex Source: GO_Central
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000305254 | 1 – 278 | TIMELESS-interacting proteinAdd BLAST | 278 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 191 | PhosphoserineBy similarity | 1 | |
Modified residuei | 219 | PhosphoserineBy similarity | 1 | |
Modified residuei | 233 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q91WA1 |
MaxQBi | Q91WA1 |
PaxDbi | Q91WA1 |
PeptideAtlasi | Q91WA1 |
PRIDEi | Q91WA1 |
PTM databases
iPTMneti | Q91WA1 |
PhosphoSitePlusi | Q91WA1 |
Expressioni
Tissue specificityi
Expressed in brain.1 Publication
Developmental stagei
Expression peaks between 11 dpc and 15 dpc. At 7.5 dpc, expressed in germ cell layers. At 14.5 dpc, expressed at highest levels in thymus, liver, gastrointestinal tract, lung and the rapidly proliferating ventricular zone of the brain.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000032397, Expressed in liver and 308 other tissues |
ExpressionAtlasi | Q91WA1, baseline and differential |
Genevisiblei | Q91WA1, MM |
Interactioni
Subunit structurei
Interacts with MCM6 and MCM7 (By similarity).
Interacts with TIMELESS, which impairs TIMELESS self-association.
Interacts with RPA2 and PRDX2.
By similarity2 PublicationsProtein-protein interaction databases
BioGRIDi | 211238, 30 interactors |
IntActi | Q91WA1, 28 interactors |
STRINGi | 10090.ENSMUSP00000034964 |
Miscellaneous databases
RNActi | Q91WA1, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 64 – 140 | Interaction with TIMELESSBy similarityAdd BLAST | 77 |
Sequence similaritiesi
Belongs to the CSM3 family.Curated
Phylogenomic databases
eggNOGi | KOG3004, Eukaryota |
GeneTreei | ENSGT00390000005764 |
HOGENOMi | CLU_074595_0_0_1 |
InParanoidi | Q91WA1 |
OMAi | NTPRAHT |
OrthoDBi | 1505397at2759 |
PhylomeDBi | Q91WA1 |
TreeFami | TF313290 |
Family and domain databases
InterProi | View protein in InterPro IPR012923, Csm3 IPR040038, TIPIN/Csm3/Swi3 |
PANTHERi | PTHR13220, PTHR13220, 1 hit |
Pfami | View protein in Pfam PF07962, Swi3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q91WA1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLEQEENGLF EIPDYEHVED ETFPPFPPPA SPERDPADAE PEEGSGSGVP
60 70 80 90 100
VPPKRTVKRN LPKLDATRLT SERGLPALRH VFDKTKFKGK GHEAEDLKTL
110 120 130 140 150
IRHMEHWAHR LFPKLQFEDF IDRVENLGNK KEVQTCLKRI RLDLPIVHED
160 170 180 190 200
FVNNNDEVGE ANGPDVSATG FDPFLTSSSD SRKFASEPTR SLTEEQQQRI
210 220 230 240 250
ERNKQLALER RQAKLLSNSQ SLENDVTVEE NSTGEDQEES NGLNIDTADG
260 270
PHDVPFASTH EEEQCKAEET QLDHTNLD
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1L1STK1 | A0A1L1STK1_MOUSE | TIMELESS-interacting protein | Tipin | 93 | Annotation score: | ||
A0A1L1SUT4 | A0A1L1SUT4_MOUSE | TIMELESS-interacting protein | Tipin | 93 | Annotation score: | ||
A0A1L1SUJ5 | A0A1L1SUJ5_MOUSE | TIMELESS-interacting protein | Tipin | 111 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 67 | T → A in BAB22798 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 265 | C → F in AAH16211 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK003451 mRNA Translation: BAB22798.1 AK003802 mRNA Translation: BAB23004.1 AK011357 mRNA Translation: BAB27565.1 AK038111 mRNA Translation: BAC29931.1 BC016211 mRNA Translation: AAH16211.1 BK001385 mRNA Translation: DAA01364.1 |
CCDSi | CCDS23279.1 |
RefSeqi | NP_001311485.1, NM_001324556.1 NP_001311486.1, NM_001324557.1 NP_001311487.1, NM_001324558.1 NP_079648.1, NM_025372.4 XP_006511403.1, XM_006511340.3 |
Genome annotation databases
Ensembli | ENSMUST00000034964; ENSMUSP00000034964; ENSMUSG00000032397 ENSMUST00000216594; ENSMUSP00000149833; ENSMUSG00000032397 |
GeneIDi | 66131 |
KEGGi | mmu:66131 |
UCSCi | uc009qbr.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK003451 mRNA Translation: BAB22798.1 AK003802 mRNA Translation: BAB23004.1 AK011357 mRNA Translation: BAB27565.1 AK038111 mRNA Translation: BAC29931.1 BC016211 mRNA Translation: AAH16211.1 BK001385 mRNA Translation: DAA01364.1 |
CCDSi | CCDS23279.1 |
RefSeqi | NP_001311485.1, NM_001324556.1 NP_001311486.1, NM_001324557.1 NP_001311487.1, NM_001324558.1 NP_079648.1, NM_025372.4 XP_006511403.1, XM_006511340.3 |
3D structure databases
SMRi | Q91WA1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 211238, 30 interactors |
IntActi | Q91WA1, 28 interactors |
STRINGi | 10090.ENSMUSP00000034964 |
PTM databases
iPTMneti | Q91WA1 |
PhosphoSitePlusi | Q91WA1 |
Proteomic databases
EPDi | Q91WA1 |
MaxQBi | Q91WA1 |
PaxDbi | Q91WA1 |
PeptideAtlasi | Q91WA1 |
PRIDEi | Q91WA1 |
Protocols and materials databases
Antibodypediai | 26122, 331 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000034964; ENSMUSP00000034964; ENSMUSG00000032397 ENSMUST00000216594; ENSMUSP00000149833; ENSMUSG00000032397 |
GeneIDi | 66131 |
KEGGi | mmu:66131 |
UCSCi | uc009qbr.1, mouse |
Organism-specific databases
CTDi | 54962 |
MGIi | MGI:1921571, Tipin |
Phylogenomic databases
eggNOGi | KOG3004, Eukaryota |
GeneTreei | ENSGT00390000005764 |
HOGENOMi | CLU_074595_0_0_1 |
InParanoidi | Q91WA1 |
OMAi | NTPRAHT |
OrthoDBi | 1505397at2759 |
PhylomeDBi | Q91WA1 |
TreeFami | TF313290 |
Enzyme and pathway databases
Reactomei | R-MMU-5693607, Processing of DNA double-strand break ends |
Miscellaneous databases
BioGRID-ORCSi | 66131, 8 hits in 19 CRISPR screens |
ChiTaRSi | Tipin, mouse |
PROi | PR:Q91WA1 |
RNActi | Q91WA1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032397, Expressed in liver and 308 other tissues |
ExpressionAtlasi | Q91WA1, baseline and differential |
Genevisiblei | Q91WA1, MM |
Family and domain databases
InterProi | View protein in InterPro IPR012923, Csm3 IPR040038, TIPIN/Csm3/Swi3 |
PANTHERi | PTHR13220, PTHR13220, 1 hit |
Pfami | View protein in Pfam PF07962, Swi3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TIPIN_MOUSE | |
Accessioni | Q91WA1Primary (citable) accession number: Q91WA1 Secondary accession number(s): Q9CQM3, Q9D1J4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 2, 2007 |
Last sequence update: | October 2, 2007 | |
Last modified: | December 2, 2020 | |
This is version 110 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families