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Protein

Anaphase-promoting complex subunit 4

Gene

Anapc4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-MMU-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-MMU-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-MMU-174154 APC/C:Cdc20 mediated degradation of Securin
R-MMU-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-MMU-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-MMU-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-MMU-176408 Regulation of APC/C activators between G1/S and early anaphase
R-MMU-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-MMU-176412 Phosphorylation of the APC/C
R-MMU-179409 APC-Cdc20 mediated degradation of Nek2A
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-MMU-69017 CDK-mediated phosphorylation and removal of Cdc6
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Anaphase-promoting complex subunit 4
Short name:
APC4
Alternative name(s):
Cyclosome subunit 4
Gene namesi
Name:Anapc4
Synonyms:D5Ertd249e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1098673 Anapc4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000645961 – 807Anaphase-promoting complex subunit 4Add BLAST807

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei469PhosphotyrosineBy similarity1
Modified residuei757PhosphoserineBy similarity1
Modified residuei758PhosphoserineBy similarity1
Cross-linki772Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei777PhosphoserineCombined sources1
Modified residuei779PhosphoserineCombined sources1
Cross-linki797Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ91W96
MaxQBiQ91W96
PaxDbiQ91W96
PRIDEiQ91W96

PTM databases

iPTMnetiQ91W96
PhosphoSitePlusiQ91W96

Expressioni

Gene expression databases

BgeeiENSMUSG00000029176 Expressed in 288 organ(s), highest expression level in primary oocyte
CleanExiMM_ANAPC4
ExpressionAtlasiQ91W96 baseline and differential
GenevisibleiQ91W96 MM

Interactioni

Subunit structurei

The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5. In the context of the APC/C complex, directly interacts with UBE2S.By similarity

GO - Molecular functioni

Protein-protein interaction databases

CORUMiQ91W96
STRINGi10090.ENSMUSP00000031072

Structurei

3D structure databases

ProteinModelPortaliQ91W96
SMRiQ91W96
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the APC4 family.Curated

Phylogenomic databases

eggNOGiKOG4640 Eukaryota
ENOG410XPXK LUCA
GeneTreeiENSGT00390000004612
HOGENOMiHOG000033987
HOVERGENiHBG044815
InParanoidiQ91W96
KOiK03351
OMAiTKYANSQ
OrthoDBiEOG091G02AJ
PhylomeDBiQ91W96
TreeFamiTF105443

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR024789 APC4
IPR024790 APC4_long_dom
IPR017169 APC4_metazoa
IPR024977 Apc4_WD40_dom
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR13260 PTHR13260, 1 hit
PfamiView protein in Pfam
PF12896 ANAPC4, 1 hit
PF12894 ANAPC4_WD40, 1 hit
PIRSFiPIRSF037303 APC4, 1 hit
SUPFAMiSSF50978 SSF50978, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q91W96-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLRFPTCFPS FRVVGEKQLP QEIIFLAWSP KRDLIALANT TGEVLLHRLA
60 70 80 90 100
SFHRVWSFPP NESTGKEVTC LAWRPDGKLL AFALADTKKI ILCDVEKPES
110 120 130 140 150
LHSFSVEAPV SCMHWTEVTV ESSVLTSFYN AEDESNLLLP KLPTLPKNYN
160 170 180 190 200
STSKIFSEEN SDEIIKLLGD VRLNILVLGG SSGFIELYAY GMFKIARVTG
210 220 230 240 250
IAGTCIALCL SSDLKSLSVV TEVSSGGESE VSYFQLETNL LYSFLPEVTR
260 270 280 290 300
MARKFTHISA LLQYINLSLT CMCEAWEEIL MQMDSRLTKF VQEKPTTTSV
310 320 330 340 350
QDEFMHLLLW GKASAELQTL LMNQLTVKGL KKLGQSIESS YSSIQKLVIS
360 370 380 390 400
HLQSGSESLL YHLSELKGMA SWKQKYEPLG LDAAGIEDAI TAVGSFILKA
410 420 430 440 450
NELLQVIDSS MKNFKAFFRW LYVAMLRMTE DHVLPELNKM TQKDITFVAE
460 470 480 490 500
FLTEHFNEAP DLYNRKGKYF NVERVGQYLK DEDDDLVSPP NTEGNQWYDF
510 520 530 540 550
LQNSTHLKES PLLFPYYPRK SLHFVKRRME NVIDQCLQKP ADVIGRSMNQ
560 570 580 590 600
AICIPLYKDA RSMDCARRLL KFPFLWNNKT SNLHYLLFTI LEDSVYKMCI
610 620 630 640 650
LRRHTDISQS VSNGLIGIKF GSFTSASADK VRRSSYSCLD AQFYDDETVT
660 670 680 690 700
VILKDSMGRE GRDRILVQLS LSLVYNSEDS DEYEFTGSYS TRLDEQGSII
710 720 730 740 750
PTRTMHFEKH WRLLESMRAQ YVAGNGLRKV SCVLSSNLRH VRVFEMDIDD
760 770 780 790 800
EWEIDESSDD EEEAGGKPVK IKEEVLSESE TEAHQDAAAL DPDVVIKVEK

LDPELDS
Length:807
Mass (Da):91,708
Last modified:December 1, 2001 - v1
Checksum:i1F99C8824BCCC7FC
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RDT6D6RDT6_MOUSE
Anaphase-promoting complex subunit ...
Anapc4
82Annotation score:
A0A0G2JF77A0A0G2JF77_MOUSE
Anaphase-promoting complex subunit ...
Anapc4
171Annotation score:
A0A0G2JGY2A0A0G2JGY2_MOUSE
Anaphase-promoting complex subunit ...
Anapc4
151Annotation score:

Sequence cautioni

The sequence BAB27965 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC002259 mRNA Translation: AAH02259.1
BC016237 mRNA Translation: AAH16237.1
BC024870 mRNA Translation: AAH24870.1
AK011994 mRNA Translation: BAB27965.1 Different initiation.
CCDSiCCDS19290.1
RefSeqiNP_077175.1, NM_024213.2
UniGeneiMm.272568

Genome annotation databases

EnsembliENSMUST00000031072; ENSMUSP00000031072; ENSMUSG00000029176
GeneIDi52206
KEGGimmu:52206
UCSCiuc008xkw.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC002259 mRNA Translation: AAH02259.1
BC016237 mRNA Translation: AAH16237.1
BC024870 mRNA Translation: AAH24870.1
AK011994 mRNA Translation: BAB27965.1 Different initiation.
CCDSiCCDS19290.1
RefSeqiNP_077175.1, NM_024213.2
UniGeneiMm.272568

3D structure databases

ProteinModelPortaliQ91W96
SMRiQ91W96
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

CORUMiQ91W96
STRINGi10090.ENSMUSP00000031072

PTM databases

iPTMnetiQ91W96
PhosphoSitePlusiQ91W96

Proteomic databases

EPDiQ91W96
MaxQBiQ91W96
PaxDbiQ91W96
PRIDEiQ91W96

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031072; ENSMUSP00000031072; ENSMUSG00000029176
GeneIDi52206
KEGGimmu:52206
UCSCiuc008xkw.1 mouse

Organism-specific databases

CTDi29945
MGIiMGI:1098673 Anapc4

Phylogenomic databases

eggNOGiKOG4640 Eukaryota
ENOG410XPXK LUCA
GeneTreeiENSGT00390000004612
HOGENOMiHOG000033987
HOVERGENiHBG044815
InParanoidiQ91W96
KOiK03351
OMAiTKYANSQ
OrthoDBiEOG091G02AJ
PhylomeDBiQ91W96
TreeFamiTF105443

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-MMU-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-MMU-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-MMU-174154 APC/C:Cdc20 mediated degradation of Securin
R-MMU-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-MMU-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-MMU-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-MMU-176408 Regulation of APC/C activators between G1/S and early anaphase
R-MMU-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-MMU-176412 Phosphorylation of the APC/C
R-MMU-179409 APC-Cdc20 mediated degradation of Nek2A
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-MMU-69017 CDK-mediated phosphorylation and removal of Cdc6
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiAnapc4 mouse
PROiPR:Q91W96
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029176 Expressed in 288 organ(s), highest expression level in primary oocyte
CleanExiMM_ANAPC4
ExpressionAtlasiQ91W96 baseline and differential
GenevisibleiQ91W96 MM

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR024789 APC4
IPR024790 APC4_long_dom
IPR017169 APC4_metazoa
IPR024977 Apc4_WD40_dom
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR13260 PTHR13260, 1 hit
PfamiView protein in Pfam
PF12896 ANAPC4, 1 hit
PF12894 ANAPC4_WD40, 1 hit
PIRSFiPIRSF037303 APC4, 1 hit
SUPFAMiSSF50978 SSF50978, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAPC4_MOUSE
AccessioniPrimary (citable) accession number: Q91W96
Secondary accession number(s): Q99LR5, Q9CZZ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: December 1, 2001
Last modified: November 7, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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