UniProtKB - Q91W36 (UBP3_MOUSE)
Protein
Ubiquitin carboxyl-terminal hydrolase 3
Gene
Usp3
Organism
Mus musculus (Mouse)
Status
Functioni
Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin (By similarity).By similarity
Catalytic activityi
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 168 | NucleophilePROSITE-ProRule annotation | 1 | |
Active sitei | 471 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 27 – 104 | UBP-typePROSITE-ProRule annotationAdd BLAST | 78 |
GO - Molecular functioni
- cysteine-type peptidase activity Source: UniProtKB-KW
- histone binding Source: MGI
- promoter-specific chromatin binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- thiol-dependent ubiquitin-specific protease activity Source: MGI
- zinc ion binding Source: InterPro
GO - Biological processi
- DNA repair Source: MGI
- histone deubiquitination Source: MGI
- mitotic cell cycle Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- regulation of protein stability Source: MGI
- ubiquitin-dependent protein catabolic process Source: InterPro
Keywordsi
Molecular function | Chromatin regulator, Hydrolase, Protease, Thiol protease |
Biological process | Cell cycle, DNA damage, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-5689880, Ub-specific processing proteases |
Protein family/group databases
MEROPSi | C19.026 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin carboxyl-terminal hydrolase 3 (EC:3.4.19.12)Alternative name(s): Deubiquitinating enzyme 3 Ubiquitin thioesterase 3 Ubiquitin-specific-processing protease 3 |
Gene namesi | Name:Usp3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2152450, Usp3 |
Subcellular locationi
Nucleus
Note: Localizes preferentially with monoubiquitinated H2A to chromatin.
Nucleus
- nuclear chromatin Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cytoplasm Source: GO_Central
- cytoplasmic ribonucleoprotein granule Source: MGI
- Flemming body Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080620 | 1 – 520 | Ubiquitin carboxyl-terminal hydrolase 3Add BLAST | 520 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q91W36 |
MaxQBi | Q91W36 |
PaxDbi | Q91W36 |
PeptideAtlasi | Q91W36 |
PRIDEi | Q91W36 |
PTM databases
iPTMneti | Q91W36 |
PhosphoSitePlusi | Q91W36 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000032376, Expressed in primary oocyte and 293 other tissues |
ExpressionAtlasi | Q91W36, baseline and differential |
Genevisiblei | Q91W36, MM |
Interactioni
Subunit structurei
Interacts (via UBP-type domain) with H2A; the interaction is less efficient than with monoubiquitinated H2A.
By similarityGO - Molecular functioni
- histone binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 231662, 1 interactor |
IntActi | Q91W36, 1 interactor |
MINTi | Q91W36 |
STRINGi | 10090.ENSMUSP00000122199 |
Miscellaneous databases
RNActi | Q91W36, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 159 – 511 | USPAdd BLAST | 353 |
Domaini
Both protease activity and an intact zinc finger are required for H2A monodeubiquitination.
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 27 – 104 | UBP-typePROSITE-ProRule annotationAdd BLAST | 78 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG1867, Eukaryota |
GeneTreei | ENSGT00940000157850 |
InParanoidi | Q91W36 |
OMAi | GHWYHFN |
OrthoDBi | 929408at2759 |
PhylomeDBi | Q91W36 |
TreeFami | TF315281 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR001394, Peptidase_C19_UCH IPR018200, USP_CS IPR028889, USP_dom IPR013083, Znf_RING/FYVE/PHD IPR001607, Znf_UBP |
Pfami | View protein in Pfam PF00443, UCH, 1 hit PF02148, zf-UBP, 1 hit |
SMARTi | View protein in SMART SM00290, ZnF_UBP, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00972, USP_1, 1 hit PS00973, USP_2, 1 hit PS50235, USP_3, 1 hit PS50271, ZF_UBP, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q91W36-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MECPHLSSSV CIAPDSAKFP NGSPSSWCCS VCRSNKSPWV CLTCSSVHCG
60 70 80 90 100
RYVNGHAKKH YEDAQIPLLN HKRSEKQEKA QHTVCMDCSS YSTYCYRCDD
110 120 130 140 150
FVVNDTKLGL VQKVREHLQN LENSAFTADR HRKRKLLENS SLNSKLLKVN
160 170 180 190 200
GSTTAICATG LRNLGNTCFM NAILQSLSNI EQFCCYFKEL PAVELRNGKT
210 220 230 240 250
AGRRTYHTRS QGDSNVSLVE EFRKTLCALW QGSQTAFSPE SLFYVVWKIM
260 270 280 290 300
PNFRGYQQQD AHEFMRYLLD HLHLELQGGF NGVSRSAILQ ENSTLSASNK
310 320 330 340 350
CCINGASTVV TAIFGGILQN EVNCLICGTE SRKFDPFLDL SLDIPSQFRS
360 370 380 390 400
KRSKNQENGP VCSLRDCLRS FTDLEELDET ELYMCHKCKK KQKSTKKFWI
410 420 430 440 450
QKLPKALCLH LKRFHWTAYL RNKVDTYVQF PLRGLDMKCY LLEPENSGPD
460 470 480 490 500
SCLYDLAAVV VHHGSGVGSG HYTAYAVHEG RWFHFNDSTV TVTDEETVGK
510 520
AKAYILFYVE RQARAGAEKL
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q8W9 | E9Q8W9_MOUSE | Ubiquitin carboxyl-terminal hydrola... | Usp3 | 482 | Annotation score: | ||
G3UZF0 | G3UZF0_MOUSE | Ubiquitin carboxyl-terminal hydrola... | Usp3 | 476 | Annotation score: | ||
D6RG67 | D6RG67_MOUSE | Ubiquitin carboxyl-terminal hydrola... | Usp3 | 49 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK031141 mRNA Translation: BAC27276.1 BC017156 mRNA Translation: AAH17156.1 |
CCDSi | CCDS23305.1 |
RefSeqi | NP_659186.1, NM_144937.4 |
Genome annotation databases
Ensembli | ENSMUST00000127569; ENSMUSP00000122199; ENSMUSG00000032376 |
GeneIDi | 235441 |
KEGGi | mmu:235441 |
UCSCi | uc009qey.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK031141 mRNA Translation: BAC27276.1 BC017156 mRNA Translation: AAH17156.1 |
CCDSi | CCDS23305.1 |
RefSeqi | NP_659186.1, NM_144937.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 231662, 1 interactor |
IntActi | Q91W36, 1 interactor |
MINTi | Q91W36 |
STRINGi | 10090.ENSMUSP00000122199 |
Protein family/group databases
MEROPSi | C19.026 |
PTM databases
iPTMneti | Q91W36 |
PhosphoSitePlusi | Q91W36 |
Proteomic databases
EPDi | Q91W36 |
MaxQBi | Q91W36 |
PaxDbi | Q91W36 |
PeptideAtlasi | Q91W36 |
PRIDEi | Q91W36 |
Protocols and materials databases
Antibodypediai | 13304, 289 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000127569; ENSMUSP00000122199; ENSMUSG00000032376 |
GeneIDi | 235441 |
KEGGi | mmu:235441 |
UCSCi | uc009qey.2, mouse |
Organism-specific databases
CTDi | 9960 |
MGIi | MGI:2152450, Usp3 |
Phylogenomic databases
eggNOGi | KOG1867, Eukaryota |
GeneTreei | ENSGT00940000157850 |
InParanoidi | Q91W36 |
OMAi | GHWYHFN |
OrthoDBi | 929408at2759 |
PhylomeDBi | Q91W36 |
TreeFami | TF315281 |
Enzyme and pathway databases
Reactomei | R-MMU-5689880, Ub-specific processing proteases |
Miscellaneous databases
BioGRID-ORCSi | 235441, 2 hits in 17 CRISPR screens |
ChiTaRSi | Usp3, mouse |
PROi | PR:Q91W36 |
RNActi | Q91W36, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032376, Expressed in primary oocyte and 293 other tissues |
ExpressionAtlasi | Q91W36, baseline and differential |
Genevisiblei | Q91W36, MM |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR001394, Peptidase_C19_UCH IPR018200, USP_CS IPR028889, USP_dom IPR013083, Znf_RING/FYVE/PHD IPR001607, Znf_UBP |
Pfami | View protein in Pfam PF00443, UCH, 1 hit PF02148, zf-UBP, 1 hit |
SMARTi | View protein in SMART SM00290, ZnF_UBP, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00972, USP_1, 1 hit PS00973, USP_2, 1 hit PS50235, USP_3, 1 hit PS50271, ZF_UBP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBP3_MOUSE | |
Accessioni | Q91W36Primary (citable) accession number: Q91W36 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 9, 2003 |
Last sequence update: | December 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families