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UniProtKB - Q91VW9 (ZKSC3_MOUSE)
Protein
Zinc finger protein with KRAB and SCAN domains 3
Gene
Zkscan3
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional factor that binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3' and acts as a repressor of autophagy. Specifically represses expression of genes involved in autophagy and lysosome biogenesis/function such as MAP1LC3B, ULK1 or WIPI2. Associates with chromatin at the ITGB4 and VEGF promoters (By similarity).
By similarityRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 313 – 335 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 341 – 363 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 369 – 391 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 397 – 419 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 425 – 447 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 479 – 501 | C2H2-type 6PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 507 – 529 | C2H2-type 7PROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- chromatin binding Source: UniProtKB
- DNA binding Source: UniProtKB
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
- identical protein binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: UniProtKB
GO - Biological processi
- autophagy Source: UniProtKB-KW
- lysosome organization Source: UniProtKB
- negative regulation of autophagy Source: UniProtKB
- negative regulation of cellular senescence Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Autophagy, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-212436, Generic Transcription Pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger protein with KRAB and SCAN domains 3Alternative name(s): SCAN-KRAB-zinc finger protein Zinc finger protein 306 Zinc finger protein 307 Zinc finger protein 47 homolog Short name: Zf47 Short name: Zfp-47 |
Gene namesi | Name:Zkscan3 Synonyms:Skz1, Zfp306, Zfp307, Zfp47 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1919989, Zkscan3 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Cytoplasm and Cytosol
- Cytoplasm By similarity
Note: Mainly localizes in the nucleus. Under starvation conditions translocates to the cytoplasm, allowing expression of target genes involved in autophagy and lysosome biogenesis/function (By similarity).By similarity
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000394861 | 1 – 553 | Zinc finger protein with KRAB and SCAN domains 3Add BLAST | 553 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 33 | PhosphoserineCombined sources | 1 | |
Modified residuei | 44 | PhosphoserineCombined sources | 1 | |
Modified residuei | 136 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 176 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 206 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 223 | PhosphoserineCombined sources | 1 | |
Modified residuei | 448 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q91VW9 |
MaxQBi | Q91VW9 |
PaxDbi | Q91VW9 |
PRIDEi | Q91VW9 |
ProteomicsDBi | 274993 |
PTM databases
iPTMneti | Q91VW9 |
PhosphoSitePlusi | Q91VW9 |
Expressioni
Tissue specificityi
Expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.1 Publication
Interactioni
GO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000068424 |
Miscellaneous databases
RNActi | Q91VW9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 51 – 133 | SCAN boxPROSITE-ProRule annotationAdd BLAST | 83 | |
Domaini | 213 – 273 | KRABAdd BLAST | 61 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 28 – 49 | DisorderedSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Belongs to the krueppel C2H2-type zinc-finger protein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 313 – 335 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 341 – 363 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 369 – 391 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 397 – 419 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 425 – 447 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 479 – 501 | C2H2-type 6PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 507 – 529 | C2H2-type 7PROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
InParanoidi | Q91VW9 |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q91VW9 |
TreeFami | TF350830 |
Family and domain databases
CDDi | cd07765, KRAB_A-box, 1 hit cd07936, SCAN, 1 hit |
Gene3Di | 1.10.4020.10, 1 hit |
InterProi | View protein in InterPro IPR001909, KRAB IPR036051, KRAB_dom_sf IPR003309, SCAN_dom IPR038269, SCAN_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF01352, KRAB, 1 hit PF02023, SCAN, 1 hit PF00096, zf-C2H2, 7 hits |
SMARTi | View protein in SMART SM00349, KRAB, 1 hit SM00431, SCAN, 1 hit SM00355, ZnF_C2H2, 7 hits |
SUPFAMi | SSF109640, SSF109640, 1 hit SSF57667, SSF57667, 4 hits |
PROSITEi | View protein in PROSITE PS50804, SCAN_BOX, 1 hit PS00028, ZINC_FINGER_C2H2_1, 7 hits PS50157, ZINC_FINGER_C2H2_2, 7 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
Q91VW9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARESRESTT LDSHSAEDQM ELLVIKVEQE ESSPLAEETS WLGSPGPDRS
60 70 80 90 100
RQRFRAFRYP EAAGPRQALS RLRELCRQWL RPDMHSKEQI LELLVLEQFL
110 120 130 140 150
TILPGELQAW VREQHPDSGE EVVALLEYLD RQLDDTPPQV PDDDDGQELL
160 170 180 190 200
CSKAVLLTSA QGSESSQMEP VEPLLKQESL GSLPSEVRVT HVGHCGEDGV
210 220 230 240 250
TATRLTSELQ GLLKMEDVAP VLSPRWTEQD SSQMNLYKDG MQEHSGSLVS
260 270 280 290 300
LDQDMQTKVR DLPRAEEYRD QKPEQTVCFL GEDTVPIPTG AEASEQEGKL
310 320 330 340 350
QAAQKSATGT RRFYCRECGK SFAQSSGLSK HKRIHTGLKP YECEECGKAF
360 370 380 390 400
IGSSALIIHQ RVHTGEKPYE CEECGKAFSH SSDLIKHQRT HTGEKPYECD
410 420 430 440 450
DCGKTFTQSC SLLEHHRIHT GEKPYQCNMC PKAFRRSSHL LRHQRTHTGD
460 470 480 490 500
KDFFVPEPYW ESQSRVESHW ENIETPVSYQ CNDCERSFSR ITSLIEHQKV
510 520 530 540 550
HTGEKPFECQ TCGKGFTRPS YLIQHQRRHT GKKTSVTVTP AVHSEVGVQL
SLN
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4J1L3 | A0A0R4J1L3_MOUSE | Zinc finger protein with KRAB and S... | Zkscan3 | 553 | Annotation score: | ||
D3YTV4 | D3YTV4_MOUSE | Zinc finger protein with KRAB and S... | Zkscan3 | 257 | Annotation score: | ||
Q5RJ53 | Q5RJ53_MOUSE | Zinc finger protein with KRAB and S... | Zkscan3 | 298 | Annotation score: | ||
A0A286YDF9 | A0A286YDF9_MOUSE | Zinc finger protein with KRAB and S... | Zkscan3 | 281 | Annotation score: | ||
A0A286YD32 | A0A286YD32_MOUSE | Zinc finger protein with KRAB and S... | Zkscan3 | 386 | Annotation score: |
Sequence cautioni
The sequence AAG00602 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 32 | S → A in BAC27333 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 32 | S → A in BAE20446 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 62 | A → T in AAG00602 (PubMed:11342224).Curated | 1 | |
Sequence conflicti | 67 | Q → R in BAE20446 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 538 | V → M in AAG00602 (PubMed:11342224).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF291722 mRNA Translation: AAG00602.1 Frameshift. AK028902 mRNA Translation: BAE20446.1 AK031286 mRNA Translation: BAC27333.1 CH466561 Genomic DNA Translation: EDL32657.1 CH466561 Genomic DNA Translation: EDL32658.1 CH466561 Genomic DNA Translation: EDL32661.1 BC007473 mRNA Translation: AAH07473.1 |
CCDSi | CCDS26274.1 |
RefSeqi | NP_001139250.1, NM_001145778.1 NP_076174.3, NM_023685.4 XP_006516852.1, XM_006516789.3 XP_006516853.1, XM_006516790.3 |
Genome annotation databases
GeneIDi | 72739 |
KEGGi | mmu:72739 |
UCSCi | uc007pqg.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF291722 mRNA Translation: AAG00602.1 Frameshift. AK028902 mRNA Translation: BAE20446.1 AK031286 mRNA Translation: BAC27333.1 CH466561 Genomic DNA Translation: EDL32657.1 CH466561 Genomic DNA Translation: EDL32658.1 CH466561 Genomic DNA Translation: EDL32661.1 BC007473 mRNA Translation: AAH07473.1 |
CCDSi | CCDS26274.1 |
RefSeqi | NP_001139250.1, NM_001145778.1 NP_076174.3, NM_023685.4 XP_006516852.1, XM_006516789.3 XP_006516853.1, XM_006516790.3 |
3D structure databases
AlphaFoldDBi | Q91VW9 |
SMRi | Q91VW9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000068424 |
PTM databases
iPTMneti | Q91VW9 |
PhosphoSitePlusi | Q91VW9 |
Proteomic databases
jPOSTi | Q91VW9 |
MaxQBi | Q91VW9 |
PaxDbi | Q91VW9 |
PRIDEi | Q91VW9 |
ProteomicsDBi | 274993 |
Protocols and materials databases
DNASUi | 72739 |
Genome annotation databases
GeneIDi | 72739 |
KEGGi | mmu:72739 |
UCSCi | uc007pqg.2, mouse |
Organism-specific databases
CTDi | 80317 |
MGIi | MGI:1919989, Zkscan3 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
InParanoidi | Q91VW9 |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q91VW9 |
TreeFami | TF350830 |
Enzyme and pathway databases
Reactomei | R-MMU-212436, Generic Transcription Pathway |
Miscellaneous databases
BioGRID-ORCSi | 72739, 4 hits in 72 CRISPR screens |
ChiTaRSi | Zkscan3, mouse |
PROi | PR:Q91VW9 |
RNActi | Q91VW9, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd07765, KRAB_A-box, 1 hit cd07936, SCAN, 1 hit |
Gene3Di | 1.10.4020.10, 1 hit |
InterProi | View protein in InterPro IPR001909, KRAB IPR036051, KRAB_dom_sf IPR003309, SCAN_dom IPR038269, SCAN_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF01352, KRAB, 1 hit PF02023, SCAN, 1 hit PF00096, zf-C2H2, 7 hits |
SMARTi | View protein in SMART SM00349, KRAB, 1 hit SM00431, SCAN, 1 hit SM00355, ZnF_C2H2, 7 hits |
SUPFAMi | SSF109640, SSF109640, 1 hit SSF57667, SSF57667, 4 hits |
PROSITEi | View protein in PROSITE PS50804, SCAN_BOX, 1 hit PS00028, ZINC_FINGER_C2H2_1, 7 hits PS50157, ZINC_FINGER_C2H2_2, 7 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | ZKSC3_MOUSE | |
Accessioni | Q91VW9Primary (citable) accession number: Q91VW9 Secondary accession number(s): Q3V3Z2, Q8CD81, Q9ESY5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 15, 2010 |
Last sequence update: | December 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 158 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families