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Protein

Microtubule-associated proteins 1A/1B light chain 3A

Gene

Map1lc3a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAutophagy, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-1632852 Macroautophagy
R-MMU-5205685 Pink/Parkin Mediated Mitophagy
R-MMU-8934903 Receptor Mediated Mitophagy

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated proteins 1A/1B light chain 3A
Alternative name(s):
Autophagy-related protein LC3 A
Autophagy-related ubiquitin-like modifier LC3 A
MAP1 light chain 3-like protein 1
MAP1A/MAP1B light chain 3 A
Short name:
MAP1A/MAP1B LC3 A
Microtubule-associated protein 1 light chain 3 alpha
Gene namesi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1915661 Map1lc3a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000171941 – 120Microtubule-associated proteins 1A/1B light chain 3AAdd BLAST120
PropeptideiPRO_0000017195121Removed in mature formCurated1

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12Phosphoserine; by PKABy similarity1
Lipidationi120Phosphatidylethanolamine amidated glycineBy similarity1

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.2 Publications
Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei120 – 121Cleavage; by ATG4BBy similarity2

Keywords - PTMi

Lipoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ91VR7
PaxDbiQ91VR7
PeptideAtlasiQ91VR7
PRIDEiQ91VR7

PTM databases

iPTMnetiQ91VR7
PhosphoSitePlusiQ91VR7

Expressioni

Gene expression databases

BgeeiENSMUSG00000027602
GenevisibleiQ91VR7 MM

Interactioni

Subunit structurei

3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins (By similarity). Interacts with TP53INP1 and TP53INP2. Directly interacts with SQSTM1; this interaction leads to MAP1LC3A recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy. Interacts with ATG13. Interacts with ULK1. Interacts with TBC1D5. Found in a complex with UBQLN1 and UBQLN2. Interacts with UBQLN4 (via STI1 1 and 2 domains). Interacts with UBQLN1 in the presence of UBQLN4. Interacts with TRIM5. Interacts with MEFV. Interacts with RETREG1, RETREG2 and RETREG3 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211680, 13 interactors
IntActiQ91VR7, 4 interactors
MINTiQ91VR7
STRINGi10090.ENSMUSP00000029128

Structurei

3D structure databases

ProteinModelPortaliQ91VR7
SMRiQ91VR7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 53Important for interaction with ATG13 and for autophagosome formationBy similarity5

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiKOG1654 Eukaryota
ENOG4111JAT LUCA
GeneTreeiENSGT00390000012937
HOGENOMiHOG000232034
HOVERGENiHBG051706
InParanoidiQ91VR7
KOiK10435
OMAiRTFADRC
OrthoDBiEOG091G18ST
PhylomeDBiQ91VR7
TreeFamiTF312964

Family and domain databases

CDDicd01611 GABARAP, 1 hit
InterProiView protein in InterPro
IPR004241 Atg8-like
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR10969 PTHR10969, 1 hit
PfamiView protein in Pfam
PF02991 Atg8, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91VR7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSDRPFKQR RSFADRCKEV QQIRDQHPSK IPVIIERYKG EKQLPVLDKT
60 70 80 90 100
KFLVPDHVNM SELVKIIRRR LQLNPTQAFF LLVNQHSMVS VSTPIADIYE
110 120
QEKDEDGFLY MVYASQETFG F
Length:121
Mass (Da):14,272
Last modified:December 1, 2001 - v1
Checksum:i48C1FBE8F7892AF3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12S → T in BAB22582 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003122 mRNA Translation: BAB22582.1
AL929588 Genomic DNA No translation available.
BC010596 mRNA Translation: AAH10596.1
CCDSiCCDS16945.1
RefSeqiNP_080011.1, NM_025735.3
UniGeneiMm.196239

Genome annotation databases

EnsembliENSMUST00000029128; ENSMUSP00000029128; ENSMUSG00000027602
GeneIDi66734
KEGGimmu:66734
UCSCiuc012cha.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMLP3A_MOUSE
AccessioniPrimary (citable) accession number: Q91VR7
Secondary accession number(s): A2AVS1, Q9DC74
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: December 1, 2001
Last modified: June 20, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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