UniProtKB - Q91VR5 (DDX1_MOUSE)
ATP-dependent RNA helicase DDX1
Ddx1
Functioni
Catalytic activityi
- EC:3.6.4.13
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 46 – 53 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- chromatin binding Source: UniProtKB
- DNA/RNA helicase activity Source: UniProtKB
- DNA binding Source: UniProtKB-KW
- double-stranded RNA binding Source: MGI
- exonuclease activity Source: UniProtKB-KW
- helicase activity Source: UniProtKB
- nuclease activity Source: UniProtKB
- poly(A) binding Source: UniProtKB
- RNA helicase activity Source: UniProtKB-EC
- transcription coregulator activity Source: UniProtKB
GO - Biological processi
- defense response to virus Source: UniProtKB-KW
- DNA duplex unwinding Source: UniProtKB
- double-strand break repair Source: UniProtKB
- innate immune response Source: UniProtKB-KW
- mRNA processing Source: UniProtKB-KW
- multicellular organism development Source: Ensembl
- nucleic acid phosphodiester bond hydrolysis Source: MGI
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- positive regulation of myeloid dendritic cell cytokine production Source: UniProtKB
- protein localization to cytoplasmic stress granule Source: MGI
- response to exogenous dsRNA Source: MGI
- response to virus Source: MGI
- tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB
Keywordsi
Molecular function | Activator, DNA-binding, Exonuclease, Helicase, Hydrolase, Nuclease, RNA-binding |
Biological process | Antiviral defense, Immunity, Innate immunity, mRNA processing, Transcription, Transcription regulation, tRNA processing |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent RNA helicase DDX1 (EC:3.6.4.13)Alternative name(s): DEAD box protein 1 |
Gene namesi | Name:Ddx1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2144727, Ddx1 |
Subcellular locationi
Cytosol
- cytosol 1 Publication
Nucleus
- Nucleus By similarity
Mitochondrion
- Mitochondrion 1 Publication
Other locations
- Cytoplasm By similarity
- Cytoplasmic granule By similarity
Note: Localized with MBNL1, TIAL1 and YBX1 in stress granules upon stress. Localized with CSTF2 in cleavage bodies. Forms large aggregates called DDX1 bodies. Relocalized into multiple foci (IR-induced foci or IRIF) after IR treatment, a process that depends on the presence of chromosomal DNA and/or RNA-DNA duplexes. Relocalized at sites of DNA double-strand breaks (DSBs) in an ATM-dependent manner after IR treatment. Colocalized with RELA in the nucleus upon TNF-alpha induction. Enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low (By similarity). Colocalizes in the cytosol with DDX21, DHX36 and TICAM1 (PubMed:21703541). Colocalizes in the mitochondria with TICAM1 and poly(I:C) RNA ligand (PubMed:21703541). The multi-helicase-TICAM1 complex may translocate to the mitochondria upon poly(I:C) stimulation (PubMed:21703541).By similarity1 Publication
Cytosol
- cytosol Source: UniProtKB
Mitochondrion
- mitochondrion Source: UniProtKB
Nucleus
- cleavage body Source: UniProtKB
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- cytoplasmic stress granule Source: MGI
- ribonucleoprotein complex Source: MGI
- tRNA-splicing ligase complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Mitochondrion, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054987 | 1 – 740 | ATP-dependent RNA helicase DDX1Add BLAST | 740 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 239 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 268 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 281 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 281 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 481 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q91VR5 |
jPOSTi | Q91VR5 |
MaxQBi | Q91VR5 |
PaxDbi | Q91VR5 |
PeptideAtlasi | Q91VR5 |
PRIDEi | Q91VR5 |
2D gel databases
REPRODUCTION-2DPAGEi | Q91VR5 |
PTM databases
iPTMneti | Q91VR5 |
PhosphoSitePlusi | Q91VR5 |
SwissPalmi | Q91VR5 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000037149, Expressed in metanephric ureteric bud and 317 other tissues |
ExpressionAtlasi | Q91VR5, baseline and differential |
Genevisiblei | Q91VR5, MM |
Interactioni
Subunit structurei
Found in a multi-helicase-TICAM1 complex at least composed of DHX36, DDX1, DDX21 and TICAM1; this complex exists in resting cells with or without poly(I:C) RNA ligand stimulation (PubMed:21703541).
Interacts with DHX36 (PubMed:21703541).
Interacts (via B30.2/SPRY domain) with DDX21 (via N-terminus); this interaction serves as bridges to TICAM1 (PubMed:21703541).
Interacts with FAM98A (via N- and C-terminus) (By similarity).
Interacts with MBNL1 (By similarity).
Interacts with CSTF2 (By similarity).
Interacts with HNRNPK (By similarity).
Interacts with ATM (By similarity).
Interacts with RELA (via C-terminus) (By similarity).
Component of the tRNA-splicing ligase complex (By similarity).
Interacts with PHF5A (via C-terminus) (PubMed:18758164).
Interacts with PQBP1 (By similarity).
Interacts with ERCC6 (By similarity).
By similarity2 PublicationsProtein-protein interaction databases
BioGRIDi | 222684, 54 interactors |
IntActi | Q91VR5, 6 interactors |
MINTi | Q91VR5 |
STRINGi | 10090.ENSMUSP00000065987 |
Miscellaneous databases
RNActi | Q91VR5, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 428 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 427 | |
Domaini | 70 – 247 | B30.2/SPRYPROSITE-ProRule annotationAdd BLAST | 178 | |
Domaini | 493 – 681 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 189 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 525 | Necessary for interaction with RELABy similarityAdd BLAST | 525 | |
Regioni | 1 – 448 | Interaction with dsRNA1 PublicationAdd BLAST | 448 | |
Regioni | 1 – 295 | Necessary for interaction with HNRNPKBy similarityAdd BLAST | 295 | |
Regioni | 525 – 740 | Necessary for interaction with HNRNPKBy similarityAdd BLAST | 216 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 370 – 373 | DEAD boxPROSITE-ProRule annotation | 4 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0349, Eukaryota |
GeneTreei | ENSGT00940000155678 |
HOGENOMi | CLU_016321_0_0_1 |
InParanoidi | Q91VR5 |
OMAi | DGVHDRD |
OrthoDBi | 973872at2759 |
PhylomeDBi | Q91VR5 |
TreeFami | TF106114 |
Family and domain databases
Gene3Di | 2.60.120.920, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR013320, ConA-like_dom_sf IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR014014, RNA_helicase_DEAD_Q_motif IPR003877, SPRY_dom |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit PF00622, SPRY, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS51192, HELICASE_ATP_BIND_1, 2 hits PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAAFSEMGVM PEIAQAVEEM DWLLPTDIQA ESIPLILGGG DVLMAAETGS
60 70 80 90 100
GKTGAFSIPV IQIVYETLKD QQEGKKGKTT IKTGASVLNK WQMNPYDRGS
110 120 130 140 150
AFAIGSDGLC CQSREVKEWH GCRGTRGLLK GKHYYEVSCH DQGLCRVGWS
160 170 180 190 200
TMQASLDLGT DKFGFGFGGT GKKSHNKQFD NYGEEFTMHD TIGCYLDIDK
210 220 230 240 250
GHVKFSKNGK DLGLAFEIPA HIKNQALFPA CVLKNAELKF NFGEEEFKFP
260 270 280 290 300
PKDGFVALSK APDNYIVKSQ HTGNAQVSQT KFLPNAPKAL IVEPSRELAE
310 320 330 340 350
QTLNNVKQFK KYIDNPKLRE LLIIGGVAAR DQLSVLDNGV DIVVGTPGRL
360 370 380 390 400
DDLVSTGKLN LSQVRFLVLD EADGLLSQGY SDFINRMHNQ IPQITCDGKR
410 420 430 440 450
LQVIVCSATL HSFDVKKLSE KIMHFPTWVD LKGEDSVPDT VHHVVVPVNP
460 470 480 490 500
KTDKLWERLG KNHIRTDDVH AKDNTRPGAN SPEMWSEAIK ILKGEYAVRA
510 520 530 540 550
IKEHKMDQAI IFCRTKIDCD NLEQYFMQQG GGPDKKGHQF SCVCLHGDRK
560 570 580 590 600
PHERKQNLER FKKGDVRFLI CTDVAARGID IHGVPYVINV TLPDEKQNYV
610 620 630 640 650
HRIGRVGRAE RMGLAISLVA TEKEKVWYHV CSNRGKGCYN TRLKEDGGCT
660 670 680 690 700
IWYNEMQLLS EIEEHLNCTI SQVEPDIKVP VDEFDGKVTY GQKRAAGGGN
710 720 730 740
YKGHVDVLAP TVQELAALEK EAQTSFLHLG YLPNQLFRTF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1Y7VM48 | A0A1Y7VM48_MOUSE | ATP-dependent RNA helicase DDX1 | Ddx1 | 190 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK028341 mRNA Translation: BAC25893.1 AK153335 mRNA Translation: BAE31914.1 AK160982 mRNA Translation: BAE36130.1 BC010624 mRNA Translation: AAH10624.1 |
CCDSi | CCDS25819.1 |
RefSeqi | NP_598801.1, NM_134040.1 |
Genome annotation databases
Ensembli | ENSMUST00000071103; ENSMUSP00000065987; ENSMUSG00000037149 |
GeneIDi | 104721 |
KEGGi | mmu:104721 |
UCSCi | uc007nbh.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK028341 mRNA Translation: BAC25893.1 AK153335 mRNA Translation: BAE31914.1 AK160982 mRNA Translation: BAE36130.1 BC010624 mRNA Translation: AAH10624.1 |
CCDSi | CCDS25819.1 |
RefSeqi | NP_598801.1, NM_134040.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 222684, 54 interactors |
IntActi | Q91VR5, 6 interactors |
MINTi | Q91VR5 |
STRINGi | 10090.ENSMUSP00000065987 |
PTM databases
iPTMneti | Q91VR5 |
PhosphoSitePlusi | Q91VR5 |
SwissPalmi | Q91VR5 |
2D gel databases
REPRODUCTION-2DPAGEi | Q91VR5 |
Proteomic databases
EPDi | Q91VR5 |
jPOSTi | Q91VR5 |
MaxQBi | Q91VR5 |
PaxDbi | Q91VR5 |
PeptideAtlasi | Q91VR5 |
PRIDEi | Q91VR5 |
Protocols and materials databases
Antibodypediai | 3224, 233 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000071103; ENSMUSP00000065987; ENSMUSG00000037149 |
GeneIDi | 104721 |
KEGGi | mmu:104721 |
UCSCi | uc007nbh.1, mouse |
Organism-specific databases
CTDi | 1653 |
MGIi | MGI:2144727, Ddx1 |
Phylogenomic databases
eggNOGi | KOG0349, Eukaryota |
GeneTreei | ENSGT00940000155678 |
HOGENOMi | CLU_016321_0_0_1 |
InParanoidi | Q91VR5 |
OMAi | DGVHDRD |
OrthoDBi | 973872at2759 |
PhylomeDBi | Q91VR5 |
TreeFami | TF106114 |
Miscellaneous databases
BioGRID-ORCSi | 104721, 9 hits in 18 CRISPR screens |
ChiTaRSi | Ddx1, mouse |
PROi | PR:Q91VR5 |
RNActi | Q91VR5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000037149, Expressed in metanephric ureteric bud and 317 other tissues |
ExpressionAtlasi | Q91VR5, baseline and differential |
Genevisiblei | Q91VR5, MM |
Family and domain databases
Gene3Di | 2.60.120.920, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR013320, ConA-like_dom_sf IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR014014, RNA_helicase_DEAD_Q_motif IPR003877, SPRY_dom |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit PF00622, SPRY, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS51192, HELICASE_ATP_BIND_1, 2 hits PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DDX1_MOUSE | |
Accessioni | Q91VR5Primary (citable) accession number: Q91VR5 Secondary accession number(s): Q3TU41 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 27, 2005 |
Last sequence update: | December 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families