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Protein

Zinc finger and BTB domain-containing protein 1

Gene

Zbtb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor (By similarity). Represses cAMP-responsive element (CRE)-mediated transcriptional activation (By similarity). In addition, has a role in translesion DNA synthesis. Requires for UV-inducible RAD18 loading, PCNA monoubiquitination, POLH recruitment to replication factories and efficient translesion DNA synthesis (By similarity). Plays a key role in the transcriptional regulation of T lymphocyte development (PubMed:22201126, PubMed:22753936).By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri216 – 242C2H2-type 1; atypicalPROSITE-ProRule annotationAdd BLAST27
Zinc fingeri448 – 470C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST23
Zinc fingeri533 – 558UBZ-typeBy similarityAdd BLAST26
Zinc fingeri578 – 600C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri606 – 628C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri634 – 656C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri662 – 684C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri686 – 709C2H2-type 7PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDifferentiation, Immunity, Innate immunity, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger and BTB domain-containing protein 1
Gene namesi
Name:Zbtb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2442326 Zbtb1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Thymus of embryos show a small number of T-cell progenitors that are unable to progress through thymic differentiation. Adult mice show vestigial thymus and lymph nodes and a reduced spleen size. In the periphery and in the spleen, display an absence of mature T-cells, a reduced number of NK cells, but a normal number of mature B-cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000477081 – 713Zinc finger and BTB domain-containing protein 1Add BLAST713

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki3Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki200Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki205Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki261Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki266Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki276Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki284Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki304Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki316Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki328Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki340Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki346Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei355PhosphoserineCombined sources1
Modified residuei356PhosphothreonineCombined sources1
Cross-linki381Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki528Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki563Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Sumoylated with SUMO2 at Lys-328 and to a lesser extent at Lys-266. Sumoylation inhibits its transcriptional repression activity and regulates its subcellular localization (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ91VL9
MaxQBiQ91VL9
PaxDbiQ91VL9
PRIDEiQ91VL9

PTM databases

iPTMnetiQ91VL9
PhosphoSitePlusiQ91VL9

Expressioni

Tissue specificityi

Expressed strongly in thymus and spleen, less in lymph nodes and peripheral blood mononuclear cells (PBMCs) and weakly in bone marrow. Strongly expressed in immature, but weakly in mature bone marrow-lymphocyte B.1 Publication

Gene expression databases

BgeeiENSMUSG00000033454 Expressed in 281 organ(s), highest expression level in blood
CleanExiMM_ZBTB1
ExpressionAtlasiQ91VL9 baseline and differential
GenevisibleiQ91VL9 MM

Interactioni

Subunit structurei

Homodimer (By similarity). Homodimer (PubMed:22753936). Interacts (via BTB domain) with TRIM28 (unphosphorylated or phosphorylated form) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi234520, 1 interactor
IntActiQ91VL9, 1 interactor
STRINGi10090.ENSMUSP00000041955

Structurei

3D structure databases

ProteinModelPortaliQ91VL9
SMRiQ91VL9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 91BTBPROSITE-ProRule annotationAdd BLAST68

Domaini

Both the BTB domain and C2H2-type motifs are necessary for transcriptional repression activity. The BTB domain is also necessary for oligomerization and efficient sumoylation. The hydrophobic cluster preceding Lys-328 enhanced sumoylation efficiency (By similarity).By similarity
The UBZ-type zinc finger domain is required for targeting ZBTB1 to UV damage sites and for PCNA monoubiquitination. UBZ-type zinc finger domain mediates binding to 'Lys-63'-linked polyubiquitin chains (in vitro).By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri216 – 242C2H2-type 1; atypicalPROSITE-ProRule annotationAdd BLAST27
Zinc fingeri448 – 470C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST23
Zinc fingeri533 – 558UBZ-typeBy similarityAdd BLAST26
Zinc fingeri578 – 600C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri606 – 628C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri634 – 656C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri662 – 684C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri686 – 709C2H2-type 7PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00850000132316
HOGENOMiHOG000056456
HOVERGENiHBG057764
InParanoidiQ91VL9
KOiK10488
OMAiSHYLSKH
OrthoDBiEOG091G032Z
PhylomeDBiQ91VL9
TreeFamiTF332229

Family and domain databases

InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR011333 SKP1/BTB/POZ_sf
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00651 BTB, 1 hit
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SM00355 ZnF_C2H2, 8 hits
SUPFAMiSSF54695 SSF54695, 1 hit
SSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 4 hits
PS50157 ZINC_FINGER_C2H2_2, 4 hits

Sequencei

Sequence statusi: Complete.

Q91VL9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKPSHSSYV LQQLNNQREW GFLCDCCIAI DDIYFQAHKA VLAACSSYFR
60 70 80 90 100
MFFMNHQHST AQLNLSNMKI SAECFDLILQ FMYLGKIMTA PSSFEQFKVA
110 120 130 140 150
MNYLQLYNVP DCLEDIQDAD CSSSKCSSSA SSRQSSKMIF GVRMYEDTVA
160 170 180 190 200
RNGNEANRWC AEPSSTVNTP HHREPEEESL QLANFPEPLF DVCKKSSVSK
210 220 230 240 250
LSTPKERVSR RFGRSFTCDS CGFGFSCEKL LDEHVLTCTN RHSYQNTTRA
260 270 280 290 300
YHRIVDIRDG KDSNIKAELA EKDSSKTFSA QPDKYREDAN QAPDDSASTT
310 320 330 340 350
GSRKSTVEAG IAGEEKSRAT ETKRIIIKME PEDIPADDMK DFNIIKVTEK
360 370 380 390 400
DCNESTDNDE LEDEPEEPFY RYYVEEDVGI KKSGRKTLKP RMSISVDERG
410 420 430 440 450
GLENMRPPNN TSPIQEDAEN ASCELCGLTI TEEDLSSHYL AKHIENICAC
460 470 480 490 500
GKCGQILVKG RQLQEHAQRC GEPQDLTMNG LGNADEKMDM EENPDEQSEI
510 520 530 540 550
RDMFVEMLDD FRDNHYQINS IQKKQLFKHS ACPFRCPNCG QRFETENLVV
560 570 580 590 600
EHMSSCLDQD MFKGAIMEEN ERDHRRKHFC NLCGKGFYQR CHLREHYTVH
610 620 630 640 650
TKEKQFVCQT CGKQFLRERQ LRLHNDMHKG MARYVCSICD QGNFRKHDHV
660 670 680 690 700
RHMISHLSGG ETICQVCFQI FPNNEQLEQH MDVHLYTCGI CGAKFNLRKD
710
MRSHYNAKHL KRT
Length:713
Mass (Da):81,952
Last modified:December 1, 2001 - v1
Checksum:i7ECD7EA1E9C88500
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti472E → K in AAH03372 (PubMed:15489334).Curated1
Sequence conflicti643N → K in BAC26603 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029762 mRNA Translation: BAC26603.1
AK035036 mRNA Translation: BAC28921.1
AK049397 mRNA Translation: BAC33733.1
BC003372 mRNA Translation: AAH03372.1
BC012239 mRNA Translation: AAH12239.1
CCDSiCCDS25992.1
RefSeqiNP_848859.1, NM_178744.2
XP_006515958.1, XM_006515895.3
UniGeneiMm.77065

Genome annotation databases

EnsembliENSMUST00000042779; ENSMUSP00000041955; ENSMUSG00000033454
GeneIDi268564
KEGGimmu:268564
UCSCiuc007nyd.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029762 mRNA Translation: BAC26603.1
AK035036 mRNA Translation: BAC28921.1
AK049397 mRNA Translation: BAC33733.1
BC003372 mRNA Translation: AAH03372.1
BC012239 mRNA Translation: AAH12239.1
CCDSiCCDS25992.1
RefSeqiNP_848859.1, NM_178744.2
XP_006515958.1, XM_006515895.3
UniGeneiMm.77065

3D structure databases

ProteinModelPortaliQ91VL9
SMRiQ91VL9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi234520, 1 interactor
IntActiQ91VL9, 1 interactor
STRINGi10090.ENSMUSP00000041955

PTM databases

iPTMnetiQ91VL9
PhosphoSitePlusiQ91VL9

Proteomic databases

EPDiQ91VL9
MaxQBiQ91VL9
PaxDbiQ91VL9
PRIDEiQ91VL9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042779; ENSMUSP00000041955; ENSMUSG00000033454
GeneIDi268564
KEGGimmu:268564
UCSCiuc007nyd.2 mouse

Organism-specific databases

CTDi22890
MGIiMGI:2442326 Zbtb1

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00850000132316
HOGENOMiHOG000056456
HOVERGENiHBG057764
InParanoidiQ91VL9
KOiK10488
OMAiSHYLSKH
OrthoDBiEOG091G032Z
PhylomeDBiQ91VL9
TreeFamiTF332229

Miscellaneous databases

ChiTaRSiZbtb1 mouse
PROiPR:Q91VL9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033454 Expressed in 281 organ(s), highest expression level in blood
CleanExiMM_ZBTB1
ExpressionAtlasiQ91VL9 baseline and differential
GenevisibleiQ91VL9 MM

Family and domain databases

InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR011333 SKP1/BTB/POZ_sf
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00651 BTB, 1 hit
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SM00355 ZnF_C2H2, 8 hits
SUPFAMiSSF54695 SSF54695, 1 hit
SSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 4 hits
PS50157 ZINC_FINGER_C2H2_2, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiZBTB1_MOUSE
AccessioniPrimary (citable) accession number: Q91VL9
Secondary accession number(s): Q8CDP7, Q99LD2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: December 1, 2001
Last modified: September 12, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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