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Protein

MKI67 FHA domain-interacting nucleolar phosphoprotein

Gene

Nifk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

Keywordsi

Molecular functionRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
MKI67 FHA domain-interacting nucleolar phosphoprotein
Alternative name(s):
Nucleolar protein interacting with the FHA domain of pKI-67
Short name:
mNIFK
Gene namesi
Name:Nifk
Synonyms:Mki67ip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1915199 Nifk

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000816302 – 317MKI67 FHA domain-interacting nucleolar phosphoproteinAdd BLAST316

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Cross-linki40Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei116Omega-N-methylated arginineBy similarity1
Cross-linki181Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki194Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei203Citrulline1 Publication1
Modified residuei219PhosphoserineBy similarity1
Modified residuei253PhosphoserineBy similarity1
Modified residuei257PhosphothreonineCombined sources1
Modified residuei261PhosphothreonineCombined sources1
Modified residuei267Omega-N-methylated arginineBy similarity1
Modified residuei268Omega-N-methylated arginineBy similarity1
Modified residuei270PhosphoserineCombined sources1
Cross-linki293Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki293Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei301PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated.By similarity
Citrullinated by PADI4.1 Publication

Keywords - PTMi

Acetylation, Citrullination, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ91VE6
PaxDbiQ91VE6
PeptideAtlasiQ91VE6
PRIDEiQ91VE6

PTM databases

iPTMnetiQ91VE6
PhosphoSitePlusiQ91VE6

Expressioni

Tissue specificityi

Expressed in brain, heart, hind limb muscles, intestine, liver, skin and spleen.1 Publication

Gene expression databases

BgeeiENSMUSG00000026377 Expressed in 292 organ(s), highest expression level in morula
ExpressionAtlasiQ91VE6 baseline and differential
GenevisibleiQ91VE6 MM

Interactioni

Subunit structurei

Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis.By similarity

Protein-protein interaction databases

BioGridi212560, 4 interactors
CORUMiQ91VE6
IntActiQ91VE6, 3 interactors
MINTiQ91VE6
STRINGi10090.ENSMUSP00000027626

Structurei

3D structure databases

ProteinModelPortaliQ91VE6
SMRiQ91VE6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 125RRMPROSITE-ProRule annotationAdd BLAST79

Phylogenomic databases

eggNOGiKOG4208 Eukaryota
ENOG4111NF9 LUCA
GeneTreeiENSGT00390000011515
HOGENOMiHOG000242795
HOVERGENiHBG057796
InParanoidiQ91VE6
KOiK14838
OMAiKCHFIPP
OrthoDBiEOG091G0RMN
PhylomeDBiQ91VE6
TreeFamiTF315137

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR021043 NIFK_FHA_Ki67_binding
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF12196 hNIFK_binding, 1 hit
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q91VE6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAGLAGPAKP SLALNPQEDS QFEKALTQIQ GRTKKPQQKK KEKLNRGVVY
60 70 80 90 100
LGHLPSTLSE SHIYNYCAQF GDISRFRLSR SKRTGNSKGY AFVEFESEDV
110 120 130 140 150
AKIVAETMDN YLFGERLLSC KFMPRKKVHK DLFSQRNALF HRPSFPAVKR
160 170 180 190 200
YNRKRGHLQM LKMEYRFKKK EKLLRKKLAA KGIDYSFPSL VLPKPKNIAV
210 220 230 240 250
AHRDSEGNQV LPDQKEGLSG EPRRKEKMMK EDISNNIPKK RKRSRRKKSS
260 270 280 290 300
VDSQGPTPVC TPTFLERRKS QVMEVGGDKD DEIILKLPVP PVKEDTQKTP
310
TSASPGGKRP RKRKSKQ
Length:317
Mass (Da):36,265
Last modified:December 1, 2001 - v1
Checksum:i08CC82E54EA2D580
GO
Isoform 2 (identifier: Q91VE6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     36-83: Missing.

Note: No experimental confirmation available.
Show »
Length:269
Mass (Da):30,646
Checksum:i4B9F1C6456B66A73
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E0CXU3E0CXU3_MOUSE
MKI67 FHA domain-interacting nucleo...
Nifk
101Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18E → G in BAC36999 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01463436 – 83Missing in isoform 2. 1 PublicationAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056870 mRNA Translation: BAB61920.1
AY030275 mRNA Translation: AAK38160.1
AK019209 mRNA Translation: BAC25583.2
AK077770 mRNA Translation: BAC36999.1
BC058559 mRNA Translation: AAH58559.1
CCDSiCCDS15222.1 [Q91VE6-1]
RefSeqiNP_080748.3, NM_026472.4 [Q91VE6-1]
UniGeneiMm.280311

Genome annotation databases

EnsembliENSMUST00000027626; ENSMUSP00000027626; ENSMUSG00000026377 [Q91VE6-1]
ENSMUST00000112688; ENSMUSP00000108308; ENSMUSG00000026377 [Q91VE6-2]
GeneIDi67949
KEGGimmu:67949
UCSCiuc007cic.2 mouse [Q91VE6-1]
uc011wql.1 mouse [Q91VE6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056870 mRNA Translation: BAB61920.1
AY030275 mRNA Translation: AAK38160.1
AK019209 mRNA Translation: BAC25583.2
AK077770 mRNA Translation: BAC36999.1
BC058559 mRNA Translation: AAH58559.1
CCDSiCCDS15222.1 [Q91VE6-1]
RefSeqiNP_080748.3, NM_026472.4 [Q91VE6-1]
UniGeneiMm.280311

3D structure databases

ProteinModelPortaliQ91VE6
SMRiQ91VE6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212560, 4 interactors
CORUMiQ91VE6
IntActiQ91VE6, 3 interactors
MINTiQ91VE6
STRINGi10090.ENSMUSP00000027626

PTM databases

iPTMnetiQ91VE6
PhosphoSitePlusiQ91VE6

Proteomic databases

EPDiQ91VE6
PaxDbiQ91VE6
PeptideAtlasiQ91VE6
PRIDEiQ91VE6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027626; ENSMUSP00000027626; ENSMUSG00000026377 [Q91VE6-1]
ENSMUST00000112688; ENSMUSP00000108308; ENSMUSG00000026377 [Q91VE6-2]
GeneIDi67949
KEGGimmu:67949
UCSCiuc007cic.2 mouse [Q91VE6-1]
uc011wql.1 mouse [Q91VE6-2]

Organism-specific databases

CTDi84365
MGIiMGI:1915199 Nifk

Phylogenomic databases

eggNOGiKOG4208 Eukaryota
ENOG4111NF9 LUCA
GeneTreeiENSGT00390000011515
HOGENOMiHOG000242795
HOVERGENiHBG057796
InParanoidiQ91VE6
KOiK14838
OMAiKCHFIPP
OrthoDBiEOG091G0RMN
PhylomeDBiQ91VE6
TreeFamiTF315137

Miscellaneous databases

ChiTaRSiNifk mouse
PROiPR:Q91VE6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026377 Expressed in 292 organ(s), highest expression level in morula
ExpressionAtlasiQ91VE6 baseline and differential
GenevisibleiQ91VE6 MM

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR021043 NIFK_FHA_Ki67_binding
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF12196 hNIFK_binding, 1 hit
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMK67I_MOUSE
AccessioniPrimary (citable) accession number: Q91VE6
Secondary accession number(s): Q8BMV9, Q8BVL4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: December 1, 2001
Last modified: November 7, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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