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Protein

Nephronectin

Gene

Npnt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functional ligand of integrin alpha-8/beta-1 in kidney development. Regulates the expression of GDNF with integrin alpha-8/beta-1 which is essential for kidney development. May also play a role in the development and function of various tissues, regulating cell adhesion, spreading and survival through the binding of several integrins.3 Publications

Miscellaneous

Was named nephronectin based on its potential role in kidney development.

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • integrin binding Source: BHF-UCL

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processCell adhesion, Differentiation
LigandCalcium

Names & Taxonomyi

Protein namesi
Recommended name:
Nephronectin
Alternative name(s):
Preosteoblast EGF-like repeat protein with MAM domain
Gene namesi
Name:Npnt
Synonyms:Neph1, Poem
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2148811 Npnt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Disruption phenotypei

Mice display renal agenesis at birth due to a developmental delay. This delay is associated with a reduced expression of Gdnf and is similar to the one found in mice lacking Itga8.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi384D → E: Reduced cell spreading- and survival-promoting activities. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000029568520 – 561NephronectinAdd BLAST542

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi56 ↔ 69PROSITE-ProRule annotation
Disulfide bondi60 ↔ 75PROSITE-ProRule annotation
Disulfide bondi77 ↔ 86PROSITE-ProRule annotation
Disulfide bondi93 ↔ 104PROSITE-ProRule annotation
Disulfide bondi100 ↔ 113PROSITE-ProRule annotation
Disulfide bondi115 ↔ 127PROSITE-ProRule annotation
Disulfide bondi173 ↔ 186PROSITE-ProRule annotation
Disulfide bondi180 ↔ 195PROSITE-ProRule annotation
Disulfide bondi197 ↔ 212PROSITE-ProRule annotation
Disulfide bondi218 ↔ 231PROSITE-ProRule annotation
Disulfide bondi225 ↔ 240PROSITE-ProRule annotation
Disulfide bondi242 ↔ 253PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ91V88
PRIDEiQ91V88

PTM databases

iPTMnetiQ91V88
PhosphoSitePlusiQ91V88

Expressioni

Tissue specificityi

Expressed in kidney (at protein level).1 Publication

Developmental stagei

Expressed from 10.5 dpc onward mainly at epithelial-mesenchymal interfaces in kidney and other tissues undergoing morphogenesis (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000040998 Expressed in 277 organ(s), highest expression level in lung
CleanExiMM_NPNT
ExpressionAtlasiQ91V88 baseline and differential
GenevisibleiQ91V88 MM

Interactioni

Subunit structurei

Homodimer and homotrimer.

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ91V88, 2 interactors
STRINGi10090.ENSMUSP00000040071

Structurei

3D structure databases

ProteinModelPortaliQ91V88
SMRiQ91V88
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 87EGF-like 1PROSITE-ProRule annotationAdd BLAST36
Domaini89 – 128EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST40
Domaini132 – 168EGF-like 3PROSITE-ProRule annotationAdd BLAST37
Domaini169 – 213EGF-like 4; calcium-bindingPROSITE-ProRule annotationAdd BLAST45
Domaini214 – 254EGF-like 5; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini420 – 561MAMPROSITE-ProRule annotationAdd BLAST142

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi382 – 384Integrin interaction3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi296 – 359Pro-richAdd BLAST64

Domaini

The MAM domain is required for localization at the cell surface.

Sequence similaritiesi

Belongs to the nephronectin family.Curated

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IR6Z Eukaryota
ENOG411039V LUCA
GeneTreeiENSGT00920000149031
HOGENOMiHOG000059638
HOVERGENiHBG108200
InParanoidiQ91V88
KOiK06824
OMAiCPRFRQC
OrthoDBiEOG091G02IF
PhylomeDBiQ91V88
TreeFamiTF330819

Family and domain databases

CDDicd06263 MAM, 1 hit
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR009030 Growth_fac_rcpt_cys_sf
IPR000998 MAM_dom
PfamiView protein in Pfam
PF07645 EGF_CA, 3 hits
PF00629 MAM, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 5 hits
SM00179 EGF_CA, 3 hits
SM00137 MAM, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
SSF57184 SSF57184, 2 hits
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 3 hits
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 3 hits
PS50026 EGF_3, 4 hits
PS01187 EGF_CA, 3 hits
PS50060 MAM_2, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q91V88-1) [UniParc]FASTAAdd to basket
Also known as: Short

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAVLLAAVLA SSLYLQVAAD FDGRWPRQIV SSIGLCRYGG RIDCCWGWAR
60 70 80 90 100
QSWGQCQPVC QPQCKHGECV GPNKCKCHPG FAGKTCNQDL NECGLKPRPC
110 120 130 140 150
KHRCMNTFGS YKCYCLNGYM LLPDGSCSSA LSCSMANCQY GCDVVKGQVR
160 170 180 190 200
CQCPSPGLQL APDGRTCVDI DECATGRVSC PRFRQCVNTF GSYICKCHTG
210 220 230 240 250
FDLMYIGGKY QCHDIDECSL GQHQCSSYAR CYNIHGSYKC QCRDGYEGDG
260 270 280 290 300
LNCVYIPKVM IEPSGPIHMP ERNGTISKGD GGHANRIPDA GSTRWPLKTP
310 320 330 340 350
YIPPVITNRP TSKPTTRPTP NPTPQPTPPP PPPLPTEPRT TPLPPTPERP
360 370 380 390 400
STRPTTIAPA TSTTTRVITV DNRIQTDPQK PRGDVFIPRQ PTNDLFEIFE
410 420 430 440 450
IERGVSADEE VKDDPGILIH SCNFDHGLCG WIREKDSDLH WETARDPAGG
460 470 480 490 500
QYLTVSAAKA PGGKAARLVL RLGHLMHSGD LCLSFRHKVT GLHSGTLQVF
510 520 530 540 550
VRKHGTHGAA LWGRNGGHGW RQTQITLRGA DVKSVIFKGE KRRGHTGEIG
560
LDDVSLKRGR C
Length:561
Mass (Da):61,490
Last modified:December 1, 2001 - v1
Checksum:i69E7ACAA0EE3F506
GO
Isoform 2 (identifier: Q91V88-2) [UniParc]FASTAAdd to basket
Also known as: Long

The sequence of this isoform differs from the canonical sequence as follows:
     58-58: P → PFYVLRQRLARIRCQLKA

Show »
Length:578
Mass (Da):63,607
Checksum:i4757DE54CC47DA4C
GO
Isoform 3 (identifier: Q91V88-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     88-88: Q → QDESFHPTPLDQGSEQPLFQPPDHQATNVPSR

Show »
Length:592
Mass (Da):64,945
Checksum:i21943B9077691396
GO
Isoform 4 (identifier: Q91V88-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     58-58: P → PFYVLRQRLARIRCQLKA
     88-88: Q → QDESFHPTPLDQGSEQPLFQPPDHQATNVPSR

Show »
Length:609
Mass (Da):67,061
Checksum:i44C45DE3EBEC4BDC
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z4D3D3Z4D3_MOUSE
Nephronectin
Npnt
532Annotation score:
D3YTX1D3YTX1_MOUSE
Nephronectin
Npnt
457Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24R → G in AAH68308 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02698958P → PFYVLRQRLARIRCQLKA in isoform 2 and isoform 4. 1 Publication1
Alternative sequenceiVSP_02699088Q → QDESFHPTPLDQGSEQPLFQ PPDHQATNVPSR in isoform 3 and isoform 4. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059656 mRNA Translation: BAB69692.1
AF397007 mRNA Translation: AAK84391.1
AF397008 mRNA Translation: AAK84392.1
AY035898 mRNA Translation: AAK96010.1
AY035899 mRNA Translation: AAK96011.1
AK050484 mRNA Translation: BAC34283.1
BC046642 mRNA Translation: AAH46642.1
BC068308 mRNA Translation: AAH68308.1
CCDSiCCDS17845.1 [Q91V88-1]
CCDS17846.1 [Q91V88-2]
CCDS71322.1 [Q91V88-3]
CCDS71323.1 [Q91V88-4]
RefSeqiNP_001025007.1, NM_001029836.2 [Q91V88-1]
NP_001274030.1, NM_001287101.1 [Q91V88-4]
NP_001274031.1, NM_001287102.1 [Q91V88-3]
NP_277060.2, NM_033525.3 [Q91V88-2]
UniGeneiMm.279310
Mm.440226

Genome annotation databases

EnsembliENSMUST00000042729; ENSMUSP00000040071; ENSMUSG00000040998 [Q91V88-2]
ENSMUST00000042744; ENSMUSP00000040684; ENSMUSG00000040998 [Q91V88-1]
ENSMUST00000093971; ENSMUSP00000091505; ENSMUSG00000040998 [Q91V88-4]
ENSMUST00000117164; ENSMUSP00000113419; ENSMUSG00000040998 [Q91V88-3]
GeneIDi114249
KEGGimmu:114249
UCSCiuc008rkc.3 mouse [Q91V88-2]
uc008rkd.3 mouse [Q91V88-1]
uc008rke.3 mouse [Q91V88-4]
uc008rkf.3 mouse [Q91V88-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059656 mRNA Translation: BAB69692.1
AF397007 mRNA Translation: AAK84391.1
AF397008 mRNA Translation: AAK84392.1
AY035898 mRNA Translation: AAK96010.1
AY035899 mRNA Translation: AAK96011.1
AK050484 mRNA Translation: BAC34283.1
BC046642 mRNA Translation: AAH46642.1
BC068308 mRNA Translation: AAH68308.1
CCDSiCCDS17845.1 [Q91V88-1]
CCDS17846.1 [Q91V88-2]
CCDS71322.1 [Q91V88-3]
CCDS71323.1 [Q91V88-4]
RefSeqiNP_001025007.1, NM_001029836.2 [Q91V88-1]
NP_001274030.1, NM_001287101.1 [Q91V88-4]
NP_001274031.1, NM_001287102.1 [Q91V88-3]
NP_277060.2, NM_033525.3 [Q91V88-2]
UniGeneiMm.279310
Mm.440226

3D structure databases

ProteinModelPortaliQ91V88
SMRiQ91V88
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ91V88, 2 interactors
STRINGi10090.ENSMUSP00000040071

PTM databases

iPTMnetiQ91V88
PhosphoSitePlusiQ91V88

Proteomic databases

PaxDbiQ91V88
PRIDEiQ91V88

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042729; ENSMUSP00000040071; ENSMUSG00000040998 [Q91V88-2]
ENSMUST00000042744; ENSMUSP00000040684; ENSMUSG00000040998 [Q91V88-1]
ENSMUST00000093971; ENSMUSP00000091505; ENSMUSG00000040998 [Q91V88-4]
ENSMUST00000117164; ENSMUSP00000113419; ENSMUSG00000040998 [Q91V88-3]
GeneIDi114249
KEGGimmu:114249
UCSCiuc008rkc.3 mouse [Q91V88-2]
uc008rkd.3 mouse [Q91V88-1]
uc008rke.3 mouse [Q91V88-4]
uc008rkf.3 mouse [Q91V88-3]

Organism-specific databases

CTDi255743
MGIiMGI:2148811 Npnt

Phylogenomic databases

eggNOGiENOG410IR6Z Eukaryota
ENOG411039V LUCA
GeneTreeiENSGT00920000149031
HOGENOMiHOG000059638
HOVERGENiHBG108200
InParanoidiQ91V88
KOiK06824
OMAiCPRFRQC
OrthoDBiEOG091G02IF
PhylomeDBiQ91V88
TreeFamiTF330819

Miscellaneous databases

PROiPR:Q91V88
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040998 Expressed in 277 organ(s), highest expression level in lung
CleanExiMM_NPNT
ExpressionAtlasiQ91V88 baseline and differential
GenevisibleiQ91V88 MM

Family and domain databases

CDDicd06263 MAM, 1 hit
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR009030 Growth_fac_rcpt_cys_sf
IPR000998 MAM_dom
PfamiView protein in Pfam
PF07645 EGF_CA, 3 hits
PF00629 MAM, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 5 hits
SM00179 EGF_CA, 3 hits
SM00137 MAM, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
SSF57184 SSF57184, 2 hits
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 3 hits
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 3 hits
PS50026 EGF_3, 4 hits
PS01187 EGF_CA, 3 hits
PS50060 MAM_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNPNT_MOUSE
AccessioniPrimary (citable) accession number: Q91V88
Secondary accession number(s): Q6NV58
, Q80VP6, Q91XL5, Q91ZD3, Q923T5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: December 1, 2001
Last modified: September 12, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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