UniProtKB - Q91V12 (BACH_MOUSE)
Protein
Cytosolic acyl coenzyme A thioester hydrolase
Gene
Acot7
Organism
Mus musculus (Mouse)
Status
Functioni
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:15288813). Acyl-coenzyme A thioesterase 7/ACOT7 preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18 (Probable). May play an important physiological function in brain (PubMed:15288813).1 Publication1 Publication
Catalytic activityi
- EC:3.1.2.21 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Kineticsi
- KM=16 µM for palmitoyl-CoA (isoform A at 30 degrees Celsius)1 Publication
- KM=12 µM for palmitoyl-CoA (isoform D at 30 degrees Celsius)1 Publication
: fatty acid metabolism Pathwayi
This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 67 | 1 Publication | 1 | |
Active sitei | 256 | 1 Publication | 1 |
GO - Molecular functioni
- acyl-CoA hydrolase activity Source: MGI
- carboxylic ester hydrolase activity Source: UniProtKB-KW
- fatty-acyl-CoA binding Source: MGI
- identical protein binding Source: IntAct
- long-chain fatty acyl-CoA binding Source: MGI
- myristoyl-CoA hydrolase activity Source: UniProtKB-EC
- palmitoyl-CoA hydrolase activity Source: MGI
- protein homodimerization activity Source: MGI
GO - Biological processi
- acyl-CoA metabolic process Source: GO_Central
- coenzyme A biosynthetic process Source: MGI
- fatty acid catabolic process Source: MGI
- fatty acid metabolic process Source: GO_Central
- long-chain fatty-acyl-CoA catabolic process Source: MGI
- medium-chain fatty acid biosynthetic process Source: MGI
- medium-chain fatty-acyl-CoA catabolic process Source: MGI
- palmitic acid biosynthetic process Source: MGI
Keywordsi
Molecular function | Hydrolase, Serine esterase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Enzyme and pathway databases
BRENDAi | 3.1.2.2, 3474 |
Reactomei | R-MMU-77289, Mitochondrial Fatty Acid Beta-Oxidation |
SABIO-RKi | Q91V12 |
UniPathwayi | UPA00199 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytosolic acyl coenzyme A thioester hydrolase (EC:3.1.2.2By similarity)Alternative name(s): Acyl-CoA thioesterase 7 Brain acyl-CoA hydrolase Short name: BACH CTE-IIa Short name: CTE-II Long chain acyl-CoA thioester hydrolase |
Gene namesi | Name:Acot7 Synonyms:Bach |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1917275, Acot7 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
Other locations
- cell body Source: MGI
- cytoplasm Source: MGI
- neuron projection Source: MGI
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 67 | N → A: Dramatic reduction in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 256 | D → A: Dramatic reduction in catalytic activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053807 | 1 – 381 | Cytosolic acyl coenzyme A thioester hydrolaseAdd BLAST | 381 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 169 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 199 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 284 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | Q91V12 |
PeptideAtlasi | Q91V12 |
PRIDEi | Q91V12 |
2D gel databases
REPRODUCTION-2DPAGEi | Q91V12 |
PTM databases
iPTMneti | Q91V12 |
PhosphoSitePlusi | Q91V12 |
SwissPalmi | Q91V12 |
Expressioni
Tissue specificityi
Widely expressed with highest levels in brain. High levels also found in thymus, large intestine and testis. Negligible in muscle and adipose tissue. In the central and peripheral nervous systems, displays a predominantly neuronal localization with highest expression in cell bodies and neurites.1 Publication
Developmental stagei
Detected in the brain as early as embryonic day (E) 11.5. The level was low until 12.5 dpc, but promptly elevated to a peak 7 days after birth. Thereafter, it declined somewhat and reached a steady-state level in adulthood. These changes in BACH expression were approximately reflected in the palmitoyl-CoA hydrolyzing activity in the developing mouse brain, and the time course was quite similar to that of microtubule-associated protein 2 (MAP2) expression. Induced during embryogenesis in association with neuronal differentiation, and persists after terminal differentiation into neurons in postnatal stages, resulting in the constitutive high expression of BACH in the adult brain in a neuron-specific manner.1 Publication
Inductioni
Up-Regulated in activated macrophages.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000028937, Expressed in ventral horn of spinal cord and 344 other tissues |
ExpressionAtlasi | Q91V12, baseline and differential |
Genevisiblei | Q91V12, MM |
Interactioni
Subunit structurei
Homohexamer.
1 PublicationBinary interactionsi
Acot7 - isoform A [Q91V12-2]
With | #Exp. | IntAct |
---|---|---|
itself | 5 | EBI-15642238,EBI-15642238 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 213825, 9 interactors |
DIPi | DIP-54691N |
IntActi | Q91V12, 4 interactors |
MINTi | Q91V12 |
STRINGi | 10090.ENSMUSP00000074827 |
Miscellaneous databases
RNActi | Q91V12, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q91V12 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q91V12 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 51 – 169 | HotDog ACOT-type 1PROSITE-ProRule annotationAdd BLAST | 119 | |
Domaini | 225 – 339 | HotDog ACOT-type 2PROSITE-ProRule annotationAdd BLAST | 115 |
Domaini
Both HotDog ACOT-type hydrolase domains are required for efficient activity.
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG2763, Eukaryota |
GeneTreei | ENSGT00940000155229 |
HOGENOMi | CLU_050164_0_1_1 |
InParanoidi | Q91V12 |
OMAi | PTDVNWG |
OrthoDBi | 1069806at2759 |
PhylomeDBi | Q91V12 |
TreeFami | TF329579 |
Family and domain databases
InterProi | View protein in InterPro IPR040170, Cytosol_ACT IPR033120, HOTDOG_ACOT IPR029069, HotDog_dom_sf IPR006683, Thioestr_dom |
PANTHERi | PTHR11049, PTHR11049, 2 hits |
Pfami | View protein in Pfam PF03061, 4HBT, 2 hits |
SUPFAMi | SSF54637, SSF54637, 2 hits |
PROSITEi | View protein in PROSITE PS51770, HOTDOG_ACOT, 2 hits |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform B (identifier: Q91V12-1) [UniParc]FASTAAdd to basket
Also known as: mBACHb
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKLLVGTLRL WEVGRQVAFS SLTPGQECSG LRKTFWAAMR AVRTRADHQK
60 70 80 90 100
LGHCVTMGRI MRPDDANVAG NVHGGTILKM IEEAGAIIST RHCNSQNGER
110 120 130 140 150
CVAALARVER TDFLSPMCIG EVAHVSAEIT YTSKHSVEVQ VHVMSENILT
160 170 180 190 200
GTKKLTNKAT LWYVPLSLKN VDKVLEVPPI VYLRQEQEEE GRKRYEAQKL
210 220 230 240 250
ERMETKWRNG DIVQPVLNPE PNTVSYSQSS LIHLVGPSDC TLHGFVHGGV
260 270 280 290 300
TMKLMDEVAG IVAARHCKTN IVTASVDAIN FHDKIRKGCV ITISGRMTFT
310 320 330 340 350
SNKSMEIEVL VDADPVVDNS QKRYRAASAF FTYVSLNQEG KPMPVPQLVP
360 370 380
ETEDEKKRFE EGKGRYLQMK AKRQGHTEPQ P
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE9PYH2 | E9PYH2_MOUSE | Cytosolic acyl coenzyme A thioester... | Acot7 | 384 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 186 | E → D in BAE31092 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000157 | 1 – 59 | MKLLV…VTMGR → MLTLHRALALRVLRKEVTEA YLREKVKQ in isoform C. 1 PublicationAdd BLAST | 59 | |
Alternative sequenceiVSP_000158 | 1 – 58 | MKLLV…CVTMG → MSGPTTDTPAAIQIC in isoform A. 3 PublicationsAdd BLAST | 58 | |
Alternative sequenceiVSP_016955 | 1 – 58 | MKLLV…CVTMG → MAPPLPSSSMAPPRLIHSGT GLLDTCSQIPPPPPSSAVAA KMSGPTTDTPAAIQIC in isoform D. 1 PublicationAdd BLAST | 58 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB088411 mRNA Translation: BAC20217.1 AB088412 mRNA Translation: BAC20218.1 AB049821 mRNA Translation: BAB61731.1 AB207243 mRNA Translation: BAD91166.1 AK152277 mRNA Translation: BAE31092.1 AL611985 Genomic DNA No translation available. AL671869 Genomic DNA No translation available. AL772240 Genomic DNA No translation available. BC013507 mRNA Translation: AAH13507.1 |
CCDSi | CCDS51390.1 [Q91V12-4] CCDS51391.2 [Q91V12-1] CCDS51392.1 [Q91V12-3] |
RefSeqi | NP_001139529.1, NM_001146057.1 [Q91V12-1] NP_001139530.1, NM_001146058.1 [Q91V12-3] NP_579926.2, NM_133348.2 [Q91V12-4] |
Genome annotation databases
Ensembli | ENSMUST00000030779; ENSMUSP00000030779; ENSMUSG00000028937 [Q91V12-1] ENSMUST00000075363; ENSMUSP00000074827; ENSMUSG00000028937 [Q91V12-4] ENSMUST00000105652; ENSMUSP00000101277; ENSMUSG00000028937 [Q91V12-3] |
GeneIDi | 70025 |
KEGGi | mmu:70025 |
UCSCi | uc008vzx.2, mouse [Q91V12-4] uc008vzz.2, mouse [Q91V12-3] uc008waa.2, mouse [Q91V12-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB088411 mRNA Translation: BAC20217.1 AB088412 mRNA Translation: BAC20218.1 AB049821 mRNA Translation: BAB61731.1 AB207243 mRNA Translation: BAD91166.1 AK152277 mRNA Translation: BAE31092.1 AL611985 Genomic DNA No translation available. AL671869 Genomic DNA No translation available. AL772240 Genomic DNA No translation available. BC013507 mRNA Translation: AAH13507.1 |
CCDSi | CCDS51390.1 [Q91V12-4] CCDS51391.2 [Q91V12-1] CCDS51392.1 [Q91V12-3] |
RefSeqi | NP_001139529.1, NM_001146057.1 [Q91V12-1] NP_001139530.1, NM_001146058.1 [Q91V12-3] NP_579926.2, NM_133348.2 [Q91V12-4] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2Q2B | X-ray | 2.50 | A/B | 203-381 | [»] | |
2V1O | X-ray | 1.78 | A/B/C/D/E/F | 59-206 | [»] | |
4ZV3 | X-ray | 3.10 | A/B/C | 55-369 | [»] | |
6VFY | X-ray | 2.60 | D/E/F | 55-369 | [»] | |
SMRi | Q91V12 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 213825, 9 interactors |
DIPi | DIP-54691N |
IntActi | Q91V12, 4 interactors |
MINTi | Q91V12 |
STRINGi | 10090.ENSMUSP00000074827 |
PTM databases
iPTMneti | Q91V12 |
PhosphoSitePlusi | Q91V12 |
SwissPalmi | Q91V12 |
2D gel databases
REPRODUCTION-2DPAGEi | Q91V12 |
Proteomic databases
PaxDbi | Q91V12 |
PeptideAtlasi | Q91V12 |
PRIDEi | Q91V12 |
Protocols and materials databases
Antibodypediai | 12967, 155 antibodies |
DNASUi | 70025 |
Genome annotation databases
Ensembli | ENSMUST00000030779; ENSMUSP00000030779; ENSMUSG00000028937 [Q91V12-1] ENSMUST00000075363; ENSMUSP00000074827; ENSMUSG00000028937 [Q91V12-4] ENSMUST00000105652; ENSMUSP00000101277; ENSMUSG00000028937 [Q91V12-3] |
GeneIDi | 70025 |
KEGGi | mmu:70025 |
UCSCi | uc008vzx.2, mouse [Q91V12-4] uc008vzz.2, mouse [Q91V12-3] uc008waa.2, mouse [Q91V12-2] |
Organism-specific databases
CTDi | 11332 |
MGIi | MGI:1917275, Acot7 |
Phylogenomic databases
eggNOGi | KOG2763, Eukaryota |
GeneTreei | ENSGT00940000155229 |
HOGENOMi | CLU_050164_0_1_1 |
InParanoidi | Q91V12 |
OMAi | PTDVNWG |
OrthoDBi | 1069806at2759 |
PhylomeDBi | Q91V12 |
TreeFami | TF329579 |
Enzyme and pathway databases
UniPathwayi | UPA00199 |
BRENDAi | 3.1.2.2, 3474 |
Reactomei | R-MMU-77289, Mitochondrial Fatty Acid Beta-Oxidation |
SABIO-RKi | Q91V12 |
Miscellaneous databases
BioGRID-ORCSi | 70025, 0 hits in 17 CRISPR screens |
ChiTaRSi | Acot7, mouse |
EvolutionaryTracei | Q91V12 |
PROi | PR:Q91V12 |
RNActi | Q91V12, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028937, Expressed in ventral horn of spinal cord and 344 other tissues |
ExpressionAtlasi | Q91V12, baseline and differential |
Genevisiblei | Q91V12, MM |
Family and domain databases
InterProi | View protein in InterPro IPR040170, Cytosol_ACT IPR033120, HOTDOG_ACOT IPR029069, HotDog_dom_sf IPR006683, Thioestr_dom |
PANTHERi | PTHR11049, PTHR11049, 2 hits |
Pfami | View protein in Pfam PF03061, 4HBT, 2 hits |
SUPFAMi | SSF54637, SSF54637, 2 hits |
PROSITEi | View protein in PROSITE PS51770, HOTDOG_ACOT, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BACH_MOUSE | |
Accessioni | Q91V12Primary (citable) accession number: Q91V12 Secondary accession number(s): A2A8K9 Q59HQ2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 12, 2003 |
Last sequence update: | February 12, 2003 | |
Last modified: | December 2, 2020 | |
This is version 159 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references