UniProtKB - Q91V04 (TRAM1_MOUSE)
Protein
Translocating chain-associated membrane protein 1
Gene
Tram1
Organism
Mus musculus (Mouse)
Status
Functioni
Stimulatory or required for the translocation of secretory proteins across the ER membrane.By similarity
GO - Biological processi
- protein insertion into ER membrane Source: GO_Central
- SRP-dependent cotranslational protein targeting to membrane, translocation Source: InterPro
Keywordsi
Biological process | Protein transport, Translocation, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Translocating chain-associated membrane protein 1 |
Gene namesi | Name:Tram1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1919515, Tram1 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- integral component of endoplasmic reticulum membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 29 | Cytoplasmic1 PublicationAdd BLAST | 29 | |
Transmembranei | 30 – 50 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 51 – 76 | Lumenal1 PublicationAdd BLAST | 26 | |
Transmembranei | 77 – 97 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 98 – 121 | Cytoplasmic1 PublicationAdd BLAST | 24 | |
Transmembranei | 122 – 142 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 143 – 159 | Lumenal1 PublicationAdd BLAST | 17 | |
Transmembranei | 160 – 180 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 181 – 192 | Cytoplasmic1 PublicationAdd BLAST | 12 | |
Transmembranei | 193 – 213 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 214 – 217 | Lumenal1 Publication | 4 | |
Transmembranei | 218 – 238 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 239 – 251 | Cytoplasmic1 PublicationAdd BLAST | 13 | |
Transmembranei | 252 – 272 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 273 – 297 | Lumenal1 PublicationAdd BLAST | 25 | |
Transmembranei | 298 – 318 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 319 – 374 | Cytoplasmic1 PublicationAdd BLAST | 56 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 56 | N → Q: Abolishes glycosylation. 1 Publication | 1 | |
Mutagenesisi | 120 | N → Q: No effect on glycosylation; when associated with Q-355. 1 Publication | 1 | |
Mutagenesisi | 355 | N → Q: No effect on glycosylation; when associated with Q-120. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000185531 | 1 – 374 | Translocating chain-associated membrane protein 1Add BLAST | 374 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 56 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Modified residuei | 365 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
EPDi | Q91V04 |
jPOSTi | Q91V04 |
MaxQBi | Q91V04 |
PaxDbi | Q91V04 |
PRIDEi | Q91V04 |
PTM databases
GlyGeni | Q91V04, 1 site |
iPTMneti | Q91V04 |
PhosphoSitePlusi | Q91V04 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000025935, Expressed in pancreas and 307 other tissues |
Genevisiblei | Q91V04, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 215264, 3 interactors |
STRINGi | 10090.ENSMUSP00000027068 |
Miscellaneous databases
RNActi | Q91V04, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 117 – 326 | TLCPROSITE-ProRule annotationAdd BLAST | 210 |
Sequence similaritiesi
Belongs to the TRAM family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1608, Eukaryota |
GeneTreei | ENSGT00510000046470 |
HOGENOMi | CLU_062830_0_0_1 |
InParanoidi | Q91V04 |
OMAi | YWLHCYP |
OrthoDBi | 831082at2759 |
PhylomeDBi | Q91V04 |
TreeFami | TF314319 |
Family and domain databases
InterProi | View protein in InterPro IPR006634, TLC-dom IPR013599, TRAM1 IPR016447, Translocation_assoc_membrane |
PANTHERi | PTHR12371, PTHR12371, 1 hit |
Pfami | View protein in Pfam PF08390, TRAM1, 1 hit PF03798, TRAM_LAG1_CLN8, 1 hit |
PIRSFi | PIRSF005449, Translocation_assoc_membrane, 1 hit |
SMARTi | View protein in SMART SM00724, TLC, 1 hit |
PROSITEi | View protein in PROSITE PS50922, TLC, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q91V04-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAIRKKSNKN PPLLSHEFLL QNHADIVSCL AMLFLLGLMF EVTAKGAIIF
60 70 80 90 100
VALQYNVTRP ATEEQATESA SLYHYGIKDL ATVLFYMLVA IIIHAIIQEY
110 120 130 140 150
VLDKINRRMH FSKTKHSKFN ESGQLSAFYL FACVWGTFIL ISENYISDPT
160 170 180 190 200
ILWRAYPHNL MTFQTKFFYI SQLAYWLHAF PELYFQKTKK EDIPRQLVYI
210 220 230 240 250
GLYLFHIAGA YLLNLNHLGL VLLVLHYFVE FLFHISRLFY FSDEKYQKGF
260 270 280 290 300
SLWAVLFVLG RLLTLILSVL TVGFGLARAE NQKLDFSTGN FNVLAVRIAV
310 320 330 340 350
LASICITQAF MMWKFINFQL RRWREHSAFQ APPVKRKPAV TKGRSSRKGT
360 370
ENGVNGTVTS NGADSPRNRK EKSS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A087WR72 | A0A087WR72_MOUSE | Translocating chain-associated memb... | Tram1 | 64 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY029764 mRNA Translation: AAK38167.1 AK088814 mRNA Translation: BAC40590.1 BC012401 mRNA Translation: AAH12401.1 |
CCDSi | CCDS14822.1 |
RefSeqi | NP_082449.1, NM_028173.5 |
Genome annotation databases
Ensembli | ENSMUST00000027068; ENSMUSP00000027068; ENSMUSG00000025935 |
GeneIDi | 72265 |
KEGGi | mmu:72265 |
UCSCi | uc007ait.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY029764 mRNA Translation: AAK38167.1 AK088814 mRNA Translation: BAC40590.1 BC012401 mRNA Translation: AAH12401.1 |
CCDSi | CCDS14822.1 |
RefSeqi | NP_082449.1, NM_028173.5 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 215264, 3 interactors |
STRINGi | 10090.ENSMUSP00000027068 |
PTM databases
GlyGeni | Q91V04, 1 site |
iPTMneti | Q91V04 |
PhosphoSitePlusi | Q91V04 |
Proteomic databases
EPDi | Q91V04 |
jPOSTi | Q91V04 |
MaxQBi | Q91V04 |
PaxDbi | Q91V04 |
PRIDEi | Q91V04 |
Protocols and materials databases
Antibodypediai | 12213, 144 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000027068; ENSMUSP00000027068; ENSMUSG00000025935 |
GeneIDi | 72265 |
KEGGi | mmu:72265 |
UCSCi | uc007ait.2, mouse |
Organism-specific databases
CTDi | 23471 |
MGIi | MGI:1919515, Tram1 |
Phylogenomic databases
eggNOGi | KOG1608, Eukaryota |
GeneTreei | ENSGT00510000046470 |
HOGENOMi | CLU_062830_0_0_1 |
InParanoidi | Q91V04 |
OMAi | YWLHCYP |
OrthoDBi | 831082at2759 |
PhylomeDBi | Q91V04 |
TreeFami | TF314319 |
Miscellaneous databases
BioGRID-ORCSi | 72265, 0 hits in 17 CRISPR screens |
ChiTaRSi | Tram1, mouse |
PROi | PR:Q91V04 |
RNActi | Q91V04, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025935, Expressed in pancreas and 307 other tissues |
Genevisiblei | Q91V04, MM |
Family and domain databases
InterProi | View protein in InterPro IPR006634, TLC-dom IPR013599, TRAM1 IPR016447, Translocation_assoc_membrane |
PANTHERi | PTHR12371, PTHR12371, 1 hit |
Pfami | View protein in Pfam PF08390, TRAM1, 1 hit PF03798, TRAM_LAG1_CLN8, 1 hit |
PIRSFi | PIRSF005449, Translocation_assoc_membrane, 1 hit |
SMARTi | View protein in SMART SM00724, TLC, 1 hit |
PROSITEi | View protein in PROSITE PS50922, TLC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRAM1_MOUSE | |
Accessioni | Q91V04Primary (citable) accession number: Q91V04 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 136 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families