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UniProtKB - Q91T40 (LAP_LSDV)
Protein
E3 ubiquitin-protein ligase LAP
Gene
LW010
Organism
Lumpy skin disease virus (LSDV)
Status
Functioni
E3 ubiquitin-protein ligase which promotes ubiquitination and subsequent degradation of host MHC-I and CD4 molecules, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell. Binds target molecules through transmembrane interaction. The result of this ubiquitination is the enhancement of the endocytosis of the target chain and the delivery to the lysosome, where it is proteolytically destroyed (By similarity).
By similarityCatalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 11 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 14 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 25 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 27 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 35 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 38 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 51 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 54 | Zinc 2PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 3 – 61 | RING-CH-typePROSITE-ProRule annotationAdd BLAST | 59 |
GO - Molecular functioni
- transferase activity Source: UniProtKB-KW
- zinc ion binding Source: InterPro
GO - Biological processi
- modulation by virus of host protein ubiquitination Source: UniProtKB-KW
- suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I Source: UniProtKB-KW
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase LAP (EC:2.3.2.27)Alternative name(s): Leukemia associated protein Short name: LAP RING-type E3 ubiquitin transferase LAPCurated |
Gene namesi | Name:LW010 |
Organismi | Lumpy skin disease virus (LSDV) |
Taxonomic identifieri | 59509 [NCBI] |
Taxonomic lineagei | Viruses › Varidnaviria › Bamfordvirae › Nucleocytoviricota › Pokkesviricetes › Chitovirales › Poxviridae › Chordopoxvirinae › Capripoxvirus |
Virus hosti | Bos taurus (Bovine) [TaxID: 9913] |
Subcellular locationi
- Host membrane Curated; Multi-pass membrane protein Curated
- host trans-Golgi network membrane
- Host early endosome membrane By similarity
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 78 | CytoplasmicSequence analysisAdd BLAST | 78 | |
Transmembranei | 79 – 99 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 100 – 121 | LumenalSequence analysisAdd BLAST | 22 | |
Transmembranei | 122 – 142 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 143 – 162 | CytoplasmicSequence analysisAdd BLAST | 20 |
Keywords - Cellular componenti
Host endosome, Host Golgi apparatus, Host membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000396003 | 1 – 162 | E3 ubiquitin-protein ligase LAPAdd BLAST | 162 |
Proteomic databases
PRIDEi | Q91T40 |
Family & Domainsi
Domaini
The RING-CH-type zinc finger domain is required for E3 ligase activity.PROSITE-ProRule annotation
Sequence similaritiesi
Belongs to the poxviridae LAP protein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 3 – 61 | RING-CH-typePROSITE-ProRule annotationAdd BLAST | 59 |
Keywords - Domaini
Transmembrane, Transmembrane helix, Zinc-fingerFamily and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR011016, Znf_RING-CH IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF12906, RINGv, 1 hit |
SMARTi | View protein in SMART SM00744, RINGv, 1 hit |
PROSITEi | View protein in PROSITE PS51292, ZF_RING_CH, 1 hit |
i Sequence
Sequence statusi: Complete.
Q91T40-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEGSDNTNTH CWICKDEYNV STNFCNCKNE FKIVHKNCLE EWINFSHNTK
60 70 80 90 100
CKICNGKYNI KKNKKSCLRW KCSFMYCNVP AICVSLICLL LLPLTILLVK
110 120 130 140 150
FNLKSMLENI ENRDLIALIS AMAYSLPCVV GFITVVHILI ALYDYYLAAK
160
SDNTTYQVYE YI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF336128 Genomic DNA Translation: AAK43550.1 AF409138 Genomic DNA Translation: AAN02734.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF336128 Genomic DNA Translation: AAK43550.1 AF409138 Genomic DNA Translation: AAN02734.1 |
3D structure databases
SMRi | Q91T40 |
ModBasei | Search... |
Proteomic databases
PRIDEi | Q91T40 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR011016, Znf_RING-CH IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF12906, RINGv, 1 hit |
SMARTi | View protein in SMART SM00744, RINGv, 1 hit |
PROSITEi | View protein in PROSITE PS51292, ZF_RING_CH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LAP_LSDV | |
Accessioni | Q91T40Primary (citable) accession number: Q91T40 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 10, 2010 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 87 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |