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Protein

Ephrin type-A receptor 4-B

Gene

epha4-b

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei652ATPPROSITE-ProRule annotation1
Active sitei745Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi626 – 634ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase
Biological processCell adhesion, Neurogenesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.1 6725

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin type-A receptor 4-B (EC:2.7.10.1)
Alternative name(s):
Pagliaccio
Tyrosine-protein kinase receptor PAG
Gene namesi
Name:epha4-b
Synonyms:pag
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 547ExtracellularSequence analysisAdd BLAST527
Transmembranei548 – 569HelicalSequence analysisAdd BLAST22
Topological domaini570 – 985CytoplasmicSequence analysisAdd BLAST416

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000001681121 – 985Ephrin type-A receptor 4-BAdd BLAST965

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi340N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi407N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei595Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei601Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei778Phosphotyrosine; by autocatalysisSequence analysis1
Modified residuei927Phosphotyrosine; by autocatalysisSequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiQ91694

Expressioni

Tissue specificityi

Localized expression in a subset of neural crest and neural tissues in embryos.

Developmental stagei

Present transiently in visceral arch 3. Also expressed in the forebrain, rhombomeres R3 and R5 of the hindbrain and in the pronephros.

Structurei

3D structure databases

ProteinModelPortaliQ91694
SMRiQ91694
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 209Eph LBDPROSITE-ProRule annotationAdd BLAST180
Domaini328 – 438Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST111
Domaini439 – 536Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST98
Domaini620 – 881Protein kinasePROSITE-ProRule annotationAdd BLAST262
Domaini910 – 974SAMPROSITE-ProRule annotationAdd BLAST65

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi983 – 985PDZ-bindingSequence analysis3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi191 – 325Cys-richAdd BLAST135

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG062180

Family and domain databases

CDDicd10482 EphR_LBD_A4, 1 hit
cd00063 FN3, 2 hits
Gene3Di2.60.120.260, 1 hit
2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR027936 Eph_TM
IPR034270 EphA4_rcpt_lig-bd
IPR001090 Ephrin_rcpt_lig-bd_dom
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR008979 Galactose-bd-like_sf
IPR009030 Growth_fac_rcpt_cys_sf
IPR013783 Ig-like_fold
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001660 SAM
IPR013761 SAM/pointed_sf
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR011641 Tyr-kin_ephrin_A/B_rcpt-like
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
IPR016257 Tyr_kinase_ephrin_rcpt
IPR001426 Tyr_kinase_rcpt_V_CS
PfamiView protein in Pfam
PF14575 EphA2_TM, 1 hit
PF01404 Ephrin_lbd, 1 hit
PF00041 fn3, 2 hits
PF07714 Pkinase_Tyr, 1 hit
PF07647 SAM_2, 1 hit
PIRSFiPIRSF000666 TyrPK_ephrin_receptor, 1 hit
PRINTSiPR00109 TYRKINASE
SMARTiView protein in SMART
SM00615 EPH_lbd, 1 hit
SM01411 Ephrin_rec_like, 1 hit
SM00060 FN3, 2 hits
SM00220 S_TKc, 1 hit
SM00454 SAM, 1 hit
SM00219 TyrKc, 1 hit
SUPFAMiSSF47769 SSF47769, 1 hit
SSF49265 SSF49265, 1 hit
SSF49785 SSF49785, 1 hit
SSF56112 SSF56112, 1 hit
SSF57184 SSF57184, 2 hits
PROSITEiView protein in PROSITE
PS01186 EGF_2, 1 hit
PS51550 EPH_LBD, 1 hit
PS50853 FN3, 2 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit
PS00790 RECEPTOR_TYR_KIN_V_1, 1 hit
PS00791 RECEPTOR_TYR_KIN_V_2, 1 hit
PS50105 SAM_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91694-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGIVHGILF CGLFGLCWAV TGSRIYPASE VTLLDSRSVQ GELGWIASPL
60 70 80 90 100
EGGWEEVSIM DEKNTPIRTY QVCNVMESSQ NNWLRTDWIP RSGAQRVYVE
110 120 130 140 150
IKFTLRDCNS LPGVMGTCKE TFNLYYYESN NDKERFIRET QYVKIDTIAA
160 170 180 190 200
DESFTQVDIG DRIMKLNTEV RDVGPLSKKG FYLAFQDVGA CIALVSVRVF
210 220 230 240 250
YKKCPLTVRN LAQFPDTITG SDTSSLVEVR GSCVDNSEEK DVPKMYCGAD
260 270 280 290 300
GEWLVPIGNC LCNAGFEEHN GGCQACKVGY YKALSTDAAC SKCPPHSYAL
310 320 330 340 350
REGSTSCTCD RGYFRADTDP ASMPCTRPPS APQNLISNVN ETSVNLEWSP
360 370 380 390 400
PQNSGGRPDV SYNLVCKRCG SDLTRCSPCG SGVHYSPQQN GLKTTKVSIN
410 420 430 440 450
DLQAHTNYTF EVWAINGVSK QNPEQDQAVS VTVTTNQAAP STVTQIQPKE
460 470 480 490 500
ITRHSVSLTW PEPERANGVI LEYEVKYYEK DQNERSYRIV KTASRSADIK
510 520 530 540 550
GLNPLTGYVF HVRARTAAGY GEFSGPFEFT TNTVPSPMIG EGTSPTVLLV
560 570 580 590 600
SVAGSIVLVV ILIAAFVISR RRSKYSKAKQ EADEEKHLNQ GVKTYVDPFT
610 620 630 640 650
YEDPNQAVRE FAKEIDASCI KIEKVIGVGE FGEVCSGRLK VPGKREIYVA
660 670 680 690 700
IKTLKAGYTD KQRRDFLSEA SIMGQFDHPN IIHLEGVVTK CKPVMIITEY
710 720 730 740 750
MENGSLDAFL RKNDGRFTVI QLVGMLRGIG SGMKYLSDMS YVHRDLAARN
760 770 780 790 800
ILVNSNLVCK VSDFGMSRVL EDDPEAAYTT RGGKIPIRWT APEAIAYRKF
810 820 830 840 850
TSASDVWSYG IVMWEVMSYG ERPYWDMSNQ DVIKAIEEGY RLPPPMDCPI
860 870 880 890 900
ALHQLMLDCW QKDRSDRPKF GQIVSMLDKL IRNPNSLKRT GLENSRTNTA
910 920 930 940 950
LLDPSSPEWS QVASVLDWLQ ASKWKRYKDN FTAAGYTSLE AVVHVNQDDL
960 970 980
TRIGISSPSH QNKILSSVQG MRTQLQQMQG RMVPV
Length:985
Mass (Da):109,730
Last modified:November 1, 1996 - v1
Checksum:iB6CFD128ACB64DF0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26099 mRNA Translation: AAA64464.1
PIRiI51549
UniGeneiXl.13

Similar proteinsi

Entry informationi

Entry nameiEPA4B_XENLA
AccessioniPrimary (citable) accession number: Q91694
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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