UniProtKB - Q91571 (EPB1A_XENLA)
Protein
Ephrin type-B receptor 1-A
Gene
ephb1-a
Organism
Xenopus laevis (African clawed frog)
Status
Functioni
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a role in axon guidance during nervous system development. May also play an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. More generally, may play a role in targeted cell migration and adhesion. Upon activation by ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively.1 Publication
Catalytic activityi
- EC:2.7.10.1PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 652 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 745 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 626 – 634 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- transmembrane-ephrin receptor activity Source: UniProtKB
GO - Biological processi
- axon guidance Source: UniProtKB
- cell chemotaxis Source: UniProtKB
- cell-substrate adhesion Source: UniProtKB
- dendritic spine development Source: UniProtKB
- dendritic spine morphogenesis Source: UniProtKB
- ephrin receptor signaling pathway Source: UniProtKB
- establishment of cell polarity Source: UniProtKB
- positive regulation of synapse assembly Source: UniProtKB
- protein autophosphorylation Source: UniProtKB
- regulation of ERK1 and ERK2 cascade Source: UniProtKB
- regulation of JNK cascade Source: UniProtKB
Keywordsi
Molecular function | Kinase, Receptor, Transferase, Tyrosine-protein kinase |
Biological process | Cell adhesion, Neurogenesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.10.1, 6725 |
Names & Taxonomyi
Protein namesi | Recommended name: Ephrin type-B receptor 1-A (EC:2.7.10.1)Alternative name(s): Tyrosine-protein kinase receptor XEK |
Gene namesi | Name:ephb1-a Synonyms:xek |
Organismi | Xenopus laevis (African clawed frog) |
Taxonomic identifieri | 8355 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein
Endosome
- Early endosome membrane By similarity
Other locations
- dendrite By similarity
Endosome
- early endosome membrane Source: UniProtKB
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
Other locations
- dendrite Source: UniProtKB-SubCell
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 20 – 542 | ExtracellularSequence analysisAdd BLAST | 523 | |
Transmembranei | 543 – 563 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 564 – 985 | CytoplasmicSequence analysisAdd BLAST | 422 |
Keywords - Cellular componenti
Cell membrane, Cell projection, Endosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000016826 | 20 – 985 | Ephrin type-B receptor 1-AAdd BLAST | 966 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 336 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 428 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 482 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Phosphorylated. Autophosphorylation is stimulated by ligands (By similarity).By similarity
Keywords - PTMi
Glycoprotein, PhosphoproteinExpressioni
Developmental stagei
Maternally expressed, it decreases at mid blastula transition and reappears at late neurulation. Expressed at higher levels in the anterior and dorsal regions of embryonic stages 16, 24 and 37. In adult it appears to be ubiquitously expressed with higher expression in brain and ovary. Expression in the brain, brachial arches, trigeminal facial ganglion, and the retina of swimming tadpole stage of development.
Interactioni
Subunit structurei
Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 21 – 203 | Eph LBDPROSITE-ProRule annotationAdd BLAST | 183 | |
Domaini | 324 – 434 | Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST | 111 | |
Domaini | 435 – 532 | Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST | 98 | |
Domaini | 620 – 883 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 264 | |
Domaini | 912 – 976 | SAMPROSITE-ProRule annotationAdd BLAST | 65 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 983 – 985 | PDZ-bindingSequence analysis | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 185 – 321 | Cys-richAdd BLAST | 137 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.PROSITE-ProRule annotation
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixFamily and domain databases
CDDi | cd10476, EphR_LBD_B1, 1 hit cd00063, FN3, 2 hits cd09551, SAM_EPH-B1, 1 hit |
Gene3Di | 1.10.150.50, 1 hit 2.60.120.260, 1 hit 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR027936, Eph_TM IPR034231, EphB1_rcpt_lig-bd IPR042819, EphB1_SAM IPR001090, Ephrin_rcpt_lig-bd_dom IPR003961, FN3_dom IPR036116, FN3_sf IPR008979, Galactose-bd-like_sf IPR009030, Growth_fac_rcpt_cys_sf IPR013783, Ig-like_fold IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001660, SAM IPR013761, SAM/pointed_sf IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom IPR016257, Tyr_kinase_ephrin_rcpt IPR001426, Tyr_kinase_rcpt_V_CS |
Pfami | View protein in Pfam PF14575, EphA2_TM, 1 hit PF01404, Ephrin_lbd, 1 hit PF00041, fn3, 1 hit PF07714, PK_Tyr_Ser-Thr, 1 hit PF00536, SAM_1, 1 hit |
PIRSFi | PIRSF000666, TyrPK_ephrin_receptor, 1 hit |
PRINTSi | PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00615, EPH_lbd, 1 hit SM00060, FN3, 2 hits SM00454, SAM, 1 hit SM00219, TyrKc, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF49265, SSF49265, 1 hit SSF49785, SSF49785, 1 hit SSF56112, SSF56112, 1 hit SSF57184, SSF57184, 1 hit |
PROSITEi | View protein in PROSITE PS01186, EGF_2, 1 hit PS51550, EPH_LBD, 1 hit PS50853, FN3, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit PS00791, RECEPTOR_TYR_KIN_V_2, 1 hit PS50105, SAM_DOMAIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q91571-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MELNVLLLLL CLSGGQVGAV EETLMDTRTA TAELGWTANP SSGWEEVSGY
60 70 80 90 100
DENLNTIRTY QVCNVFGPKQ NNWLLTTFIP RRGAHRVYVE MRFTVRDCSS
110 120 130 140 150
LPNVPGSCKE TFNLYYYETD SNIENKISTF WNESPYLKVD TIAADESFSQ
160 170 180 190 200
VDFGGRLMKV NTEVRSFGPL TRSGFYLAFQ DYGACMSLLS VRVFFKEMPS
210 220 230 240 250
VVQNLLVFPE TMTGAESTSL VIARGTCIPN AEEVDVPIKL YCNGDGEWMV
260 270 280 290 300
PIGKCTCKAG YEPENHVVCK ACPAAMFKAN QGMGICAQCP ANSRSTSEAS
310 320 330 340 350
PICICRNGYY RADFDTPEAP CTSVPSGPRN VISIVNETAI TLEWHPPRET
360 370 380 390 400
GGRDDVDYNI VCKKCRADRR ACSRCDDNVD FVPRQLGLTD TRVFISNLWA
410 420 430 440 450
HTPYTFETQA VNGVTNKSPF PPQHVSVNIT TNQAAPSSVP IMHQVKATMK
460 470 480 490 500
SITLSWPQQE QPNGIILDYE IRYYEKDHHE FNSSLARSQT NTARRTGGRV
510 520 530 540 550
WMFMSVQVRA RTVAGYGKFS SKCGFQTLTA EDYKSELREQ LPLTGSAAAG
560 570 580 590 600
VVFIVSLVAI SIVCSRKRTY SKEAVYSDKL QHYSTGRGSP GMKIYIDPFT
610 620 630 640 650
YEDPNEAVRE FAKEIDVSFV KIEEVIGAGE FGEVYKGRLK LPSKREISVA
660 670 680 690 700
IKTLKAGYSE KQRRDFLSEA SIMGQFDHPN IIRLEGVVTK SRPVMIITEF
710 720 730 740 750
MENGALDSFL RQNDGQFTVI QLVGMLRGIA AGMKYLSEMN YVHRDLAARN
760 770 780 790 800
ILVNSNLVCK VSDFGLSRYL QDDTSDPTYT SSLGGKIPVR WTAQEAIAYR
810 820 830 840 850
KFTSASDVWS YGIVMWEVMS YGERPYWTMS NQDVINAIEQ DYRLPPPMDC
860 870 880 890 900
PAALHQLMLD CWQKDRNSRP RLAEIVNTLR PMIRNPASLK TVATIPAVPS
910 920 930 940 950
QPLLDRSIPD ISAFTSVDDW LSAIKMGQYR DNFLSSGFTS LQLVAQMTSE
960 970 980
DLLRIGITLA GHQKKILNSI QSMRVQITQS PTSIA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14164 mRNA Translation: AAA74888.1 |
PIRi | I51672 |
RefSeqi | NP_001084070.1, NM_001090601.1 |
Genome annotation databases
GeneIDi | 399288 |
KEGGi | xla:399288 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14164 mRNA Translation: AAA74888.1 |
PIRi | I51672 |
RefSeqi | NP_001084070.1, NM_001090601.1 |
3D structure databases
SMRi | Q91571 |
ModBasei | Search... |
Genome annotation databases
GeneIDi | 399288 |
KEGGi | xla:399288 |
Organism-specific databases
CTDi | 399288 |
Enzyme and pathway databases
BRENDAi | 2.7.10.1, 6725 |
Family and domain databases
CDDi | cd10476, EphR_LBD_B1, 1 hit cd00063, FN3, 2 hits cd09551, SAM_EPH-B1, 1 hit |
Gene3Di | 1.10.150.50, 1 hit 2.60.120.260, 1 hit 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR027936, Eph_TM IPR034231, EphB1_rcpt_lig-bd IPR042819, EphB1_SAM IPR001090, Ephrin_rcpt_lig-bd_dom IPR003961, FN3_dom IPR036116, FN3_sf IPR008979, Galactose-bd-like_sf IPR009030, Growth_fac_rcpt_cys_sf IPR013783, Ig-like_fold IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001660, SAM IPR013761, SAM/pointed_sf IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom IPR016257, Tyr_kinase_ephrin_rcpt IPR001426, Tyr_kinase_rcpt_V_CS |
Pfami | View protein in Pfam PF14575, EphA2_TM, 1 hit PF01404, Ephrin_lbd, 1 hit PF00041, fn3, 1 hit PF07714, PK_Tyr_Ser-Thr, 1 hit PF00536, SAM_1, 1 hit |
PIRSFi | PIRSF000666, TyrPK_ephrin_receptor, 1 hit |
PRINTSi | PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00615, EPH_lbd, 1 hit SM00060, FN3, 2 hits SM00454, SAM, 1 hit SM00219, TyrKc, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF49265, SSF49265, 1 hit SSF49785, SSF49785, 1 hit SSF56112, SSF56112, 1 hit SSF57184, SSF57184, 1 hit |
PROSITEi | View protein in PROSITE PS01186, EGF_2, 1 hit PS51550, EPH_LBD, 1 hit PS50853, FN3, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit PS00791, RECEPTOR_TYR_KIN_V_2, 1 hit PS50105, SAM_DOMAIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EPB1A_XENLA | |
Accessioni | Q91571Primary (citable) accession number: Q91571 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | November 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |