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Protein

Ephrin type-B receptor 1-A

Gene

ephb1-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a role in axon guidance during nervous system development. May also play an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. More generally, may play a role in targeted cell migration and adhesion. Upon activation by ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei652ATPPROSITE-ProRule annotation1
Active sitei745Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi626 – 634ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Receptor, Transferase, Tyrosine-protein kinase
Biological processCell adhesion, Neurogenesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.1 6725

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin type-B receptor 1-A (EC:2.7.10.1)
Alternative name(s):
Tyrosine-protein kinase receptor XEK
Gene namesi
Name:ephb1-a
Synonyms:xek
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-865799 ephb1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 542ExtracellularSequence analysisAdd BLAST523
Transmembranei543 – 563HelicalSequence analysisAdd BLAST21
Topological domaini564 – 985CytoplasmicSequence analysisAdd BLAST422

Keywords - Cellular componenti

Cell membrane, Cell projection, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000001682620 – 985Ephrin type-B receptor 1-AAdd BLAST966

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi336N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi428N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi482N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Phosphorylated. Autophosphorylation is stimulated by ligands (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiQ91571

Expressioni

Developmental stagei

Maternally expressed, it decreases at mid blastula transition and reappears at late neurulation. Expressed at higher levels in the anterior and dorsal regions of embryonic stages 16, 24 and 37. In adult it appears to be ubiquitously expressed with higher expression in brain and ovary. Expression in the brain, brachial arches, trigeminal facial ganglion, and the retina of swimming tadpole stage of development.

Interactioni

Subunit structurei

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ91571
SMRiQ91571
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 203Eph LBDPROSITE-ProRule annotationAdd BLAST183
Domaini324 – 434Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST111
Domaini435 – 532Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST98
Domaini620 – 883Protein kinasePROSITE-ProRule annotationAdd BLAST264
Domaini912 – 976SAMPROSITE-ProRule annotationAdd BLAST65

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi983 – 985PDZ-bindingSequence analysis3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi185 – 321Cys-richAdd BLAST137

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG062180
KOiK05110

Family and domain databases

CDDicd10476 EphR_LBD_B1, 1 hit
cd00063 FN3, 2 hits
Gene3Di2.60.120.260, 1 hit
2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR027936 Eph_TM
IPR034231 EphB1_rcpt_lig-bd
IPR001090 Ephrin_rcpt_lig-bd_dom
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR008979 Galactose-bd-like_sf
IPR009030 Growth_fac_rcpt_cys_sf
IPR013783 Ig-like_fold
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001660 SAM
IPR013761 SAM/pointed_sf
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR011641 Tyr-kin_ephrin_A/B_rcpt-like
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
IPR016257 Tyr_kinase_ephrin_rcpt
IPR001426 Tyr_kinase_rcpt_V_CS
PfamiView protein in Pfam
PF14575 EphA2_TM, 1 hit
PF01404 Ephrin_lbd, 1 hit
PF00041 fn3, 1 hit
PF07714 Pkinase_Tyr, 1 hit
PF00536 SAM_1, 1 hit
PIRSFiPIRSF000666 TyrPK_ephrin_receptor, 1 hit
PRINTSiPR00109 TYRKINASE
SMARTiView protein in SMART
SM00615 EPH_lbd, 1 hit
SM01411 Ephrin_rec_like, 1 hit
SM00060 FN3, 2 hits
SM00454 SAM, 1 hit
SM00219 TyrKc, 1 hit
SUPFAMiSSF47769 SSF47769, 1 hit
SSF49265 SSF49265, 1 hit
SSF49785 SSF49785, 1 hit
SSF56112 SSF56112, 1 hit
SSF57184 SSF57184, 2 hits
PROSITEiView protein in PROSITE
PS01186 EGF_2, 1 hit
PS51550 EPH_LBD, 1 hit
PS50853 FN3, 2 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit
PS00791 RECEPTOR_TYR_KIN_V_2, 1 hit
PS50105 SAM_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91571-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MELNVLLLLL CLSGGQVGAV EETLMDTRTA TAELGWTANP SSGWEEVSGY
60 70 80 90 100
DENLNTIRTY QVCNVFGPKQ NNWLLTTFIP RRGAHRVYVE MRFTVRDCSS
110 120 130 140 150
LPNVPGSCKE TFNLYYYETD SNIENKISTF WNESPYLKVD TIAADESFSQ
160 170 180 190 200
VDFGGRLMKV NTEVRSFGPL TRSGFYLAFQ DYGACMSLLS VRVFFKEMPS
210 220 230 240 250
VVQNLLVFPE TMTGAESTSL VIARGTCIPN AEEVDVPIKL YCNGDGEWMV
260 270 280 290 300
PIGKCTCKAG YEPENHVVCK ACPAAMFKAN QGMGICAQCP ANSRSTSEAS
310 320 330 340 350
PICICRNGYY RADFDTPEAP CTSVPSGPRN VISIVNETAI TLEWHPPRET
360 370 380 390 400
GGRDDVDYNI VCKKCRADRR ACSRCDDNVD FVPRQLGLTD TRVFISNLWA
410 420 430 440 450
HTPYTFETQA VNGVTNKSPF PPQHVSVNIT TNQAAPSSVP IMHQVKATMK
460 470 480 490 500
SITLSWPQQE QPNGIILDYE IRYYEKDHHE FNSSLARSQT NTARRTGGRV
510 520 530 540 550
WMFMSVQVRA RTVAGYGKFS SKCGFQTLTA EDYKSELREQ LPLTGSAAAG
560 570 580 590 600
VVFIVSLVAI SIVCSRKRTY SKEAVYSDKL QHYSTGRGSP GMKIYIDPFT
610 620 630 640 650
YEDPNEAVRE FAKEIDVSFV KIEEVIGAGE FGEVYKGRLK LPSKREISVA
660 670 680 690 700
IKTLKAGYSE KQRRDFLSEA SIMGQFDHPN IIRLEGVVTK SRPVMIITEF
710 720 730 740 750
MENGALDSFL RQNDGQFTVI QLVGMLRGIA AGMKYLSEMN YVHRDLAARN
760 770 780 790 800
ILVNSNLVCK VSDFGLSRYL QDDTSDPTYT SSLGGKIPVR WTAQEAIAYR
810 820 830 840 850
KFTSASDVWS YGIVMWEVMS YGERPYWTMS NQDVINAIEQ DYRLPPPMDC
860 870 880 890 900
PAALHQLMLD CWQKDRNSRP RLAEIVNTLR PMIRNPASLK TVATIPAVPS
910 920 930 940 950
QPLLDRSIPD ISAFTSVDDW LSAIKMGQYR DNFLSSGFTS LQLVAQMTSE
960 970 980
DLLRIGITLA GHQKKILNSI QSMRVQITQS PTSIA
Length:985
Mass (Da):110,104
Last modified:November 1, 1996 - v1
Checksum:iBE72CD1BFF51E623
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14164 mRNA Translation: AAA74888.1
PIRiI51672
RefSeqiNP_001084070.1, NM_001090601.1
UniGeneiXl.1028

Genome annotation databases

GeneIDi399288
KEGGixla:399288

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14164 mRNA Translation: AAA74888.1
PIRiI51672
RefSeqiNP_001084070.1, NM_001090601.1
UniGeneiXl.1028

3D structure databases

ProteinModelPortaliQ91571
SMRiQ91571
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ91571

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi399288
KEGGixla:399288

Organism-specific databases

CTDi399288
XenbaseiXB-GENE-865799 ephb1

Phylogenomic databases

HOVERGENiHBG062180
KOiK05110

Enzyme and pathway databases

BRENDAi2.7.10.1 6725

Family and domain databases

CDDicd10476 EphR_LBD_B1, 1 hit
cd00063 FN3, 2 hits
Gene3Di2.60.120.260, 1 hit
2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR027936 Eph_TM
IPR034231 EphB1_rcpt_lig-bd
IPR001090 Ephrin_rcpt_lig-bd_dom
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR008979 Galactose-bd-like_sf
IPR009030 Growth_fac_rcpt_cys_sf
IPR013783 Ig-like_fold
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001660 SAM
IPR013761 SAM/pointed_sf
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR011641 Tyr-kin_ephrin_A/B_rcpt-like
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
IPR016257 Tyr_kinase_ephrin_rcpt
IPR001426 Tyr_kinase_rcpt_V_CS
PfamiView protein in Pfam
PF14575 EphA2_TM, 1 hit
PF01404 Ephrin_lbd, 1 hit
PF00041 fn3, 1 hit
PF07714 Pkinase_Tyr, 1 hit
PF00536 SAM_1, 1 hit
PIRSFiPIRSF000666 TyrPK_ephrin_receptor, 1 hit
PRINTSiPR00109 TYRKINASE
SMARTiView protein in SMART
SM00615 EPH_lbd, 1 hit
SM01411 Ephrin_rec_like, 1 hit
SM00060 FN3, 2 hits
SM00454 SAM, 1 hit
SM00219 TyrKc, 1 hit
SUPFAMiSSF47769 SSF47769, 1 hit
SSF49265 SSF49265, 1 hit
SSF49785 SSF49785, 1 hit
SSF56112 SSF56112, 1 hit
SSF57184 SSF57184, 2 hits
PROSITEiView protein in PROSITE
PS01186 EGF_2, 1 hit
PS51550 EPH_LBD, 1 hit
PS50853 FN3, 2 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit
PS00791 RECEPTOR_TYR_KIN_V_2, 1 hit
PS50105 SAM_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEPB1A_XENLA
AccessioniPrimary (citable) accession number: Q91571
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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