UniProtKB - Q91437 (PYR1_SQUAC)
Protein
CAD protein
Gene
CAD
Organism
Squalus acanthias (Spiny dogfish)
Status
Functioni
This protein is a 'fusion' protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).
Miscellaneous
GATase (glutamine amidotransferase) and CPSase (carbamoyl phosphate synthase) form together the glutamine-dependent CPSase (GD-CPSase) (EC 6.3.5.5).
Catalytic activityi
- 2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H+ + L-glutamate + phosphateEC:6.3.5.5
- EC:2.1.3.2
- EC:3.5.2.3
Cofactori
Zn2+By similarityNote: Binds 3 Zn2+ ions per subunit (for dihydroorotase activity).By similarity
Activity regulationi
Allosterically regulated and controlled by phosphorylation. 5-phosphoribose 1-diphosphate is an activator while UMP is an inhibitor of the CPSase reaction (By similarity).By similarity
: UMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1, 2 and 3 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- CAD protein (CAD)
- CAD protein (CAD)
- CAD protein (CAD)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 252 | For GATase activityBy similarity | 1 | |
Active sitei | 336 | For GATase activityBy similarity | 1 | |
Active sitei | 338 | For GATase activityBy similarity | 1 | |
Metal bindingi | 1478 | Zinc 1; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 1478 | Zinc 2; via pros nitrogenBy similarity | 1 | |
Metal bindingi | 1480 | Zinc 1; via tele nitrogenBy similarity | 1 | |
Binding sitei | 1482 | N-carbamoyl-L-aspartateBy similarity | 1 | |
Binding sitei | 1512 | N-carbamoyl-L-aspartateBy similarity | 1 | |
Metal bindingi | 1563 | Zinc 1; via carbamate groupBy similarity | 1 | |
Metal bindingi | 1563 | Zinc 3; via carbamate groupBy similarity | 1 | |
Metal bindingi | 1597 | Zinc 3; via pros nitrogenBy similarity | 1 | |
Metal bindingi | 1620 | Zinc 2By similarity | 1 | |
Metal bindingi | 1621 | Zinc 3; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 1644 | Zinc 2By similarity | 1 | |
Binding sitei | 1668 | N-carbamoyl-L-aspartate; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 1693 | Zinc 1By similarity | 1 | |
Binding sitei | 1693 | N-carbamoyl-L-aspartateBy similarity | 1 | |
Binding sitei | 1697 | N-carbamoyl-L-aspartateBy similarity | 1 |
GO - Molecular functioni
- amino acid binding Source: InterPro
- aspartate carbamoyltransferase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
- carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
- dihydroorotase activity Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- 'de novo' pyrimidine nucleobase biosynthetic process Source: UniProtKB
- 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
- glutamine metabolic process Source: InterPro
Keywordsi
Molecular function | Allosteric enzyme, Hydrolase, Ligase, Multifunctional enzyme, Transferase |
Biological process | Pyrimidine biosynthesis |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00070;UER00115 UPA00070;UER00116 UPA00070;UER00117 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:CAD |
Organismi | Squalus acanthias (Spiny dogfish) |
Taxonomic identifieri | 7797 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Chondrichthyes › Elasmobranchii › Squalomorphii › Squaliformes › Squalidae › Squalus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000199508 | 1 – 2242 | CAD proteinAdd BLAST | 2242 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1563 | N6-carboxylysineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinExpressioni
Tissue specificityi
Present in the testis but not in the liver.1 Publication
Interactioni
Subunit structurei
Homohexamer.
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 177 – 363 | Glutamine amidotransferase type-1Add BLAST | 187 | |
Domaini | 522 – 714 | ATP-grasp 1Add BLAST | 193 | |
Domaini | 1057 – 1248 | ATP-grasp 2Add BLAST | 192 | |
Domaini | 1313 – 1469 | MGS-likePROSITE-ProRule annotationAdd BLAST | 157 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 365 | GATase (Glutamine amidotransferase)Add BLAST | 365 | |
Regioni | 366 – 397 | LinkerAdd BLAST | 32 | |
Regioni | 398 – 1462 | CPSase (Carbamoyl-phosphate synthase)Add BLAST | 1065 | |
Regioni | 398 – 937 | CPSase AAdd BLAST | 540 | |
Regioni | 938 – 1462 | CPSase BAdd BLAST | 525 | |
Regioni | 1463 – 1796 | DHOase (dihydroorotase)Add BLAST | 334 | |
Regioni | 1797 – 1934 | LinkerAdd BLAST | 138 | |
Regioni | 1935 – 2242 | ATCase (Aspartate transcarbamylase)Add BLAST | 308 |
Sequence similaritiesi
In the central section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily.Curated
Keywords - Domaini
RepeatFamily and domain databases
CDDi | cd01744, GATase1_CPSase, 1 hit |
Gene3Di | 1.10.1030.10, 1 hit 3.30.1490.20, 1 hit 3.40.50.1370, 2 hits 3.40.50.1380, 1 hit 3.40.50.880, 1 hit 3.50.30.20, 1 hit |
HAMAPi | MF_00001, Asp_carb_tr, 1 hit MF_01209, CPSase_S_chain, 1 hit |
InterProi | View protein in InterPro IPR006680, Amidohydro-rel IPR006132, Asp/Orn_carbamoyltranf_P-bd IPR006130, Asp/Orn_carbamoylTrfase IPR036901, Asp/Orn_carbamoylTrfase_sf IPR002082, Asp_carbamoyltransf IPR006131, Asp_carbamoyltransf_Asp/Orn-bd IPR011761, ATP-grasp IPR013815, ATP_grasp_subdomain_1 IPR006275, CarbamoylP_synth_lsu IPR005480, CarbamoylP_synth_lsu_oligo IPR036897, CarbamoylP_synth_lsu_oligo_sf IPR006274, CarbamoylP_synth_ssu IPR002474, CarbamoylP_synth_ssu_N IPR036480, CarbP_synth_ssu_N_sf IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR005483, CbamoylP_synth_lsu_CPSase_dom IPR029062, Class_I_gatase-like IPR035686, CPSase_GATase1 IPR002195, Dihydroorotase_CS IPR017926, GATASE IPR011059, Metal-dep_hydrolase_composite IPR032466, Metal_Hydrolase IPR011607, MGS-like_dom IPR036914, MGS-like_dom_sf IPR016185, PreATP-grasp_dom_sf |
Pfami | View protein in Pfam PF01979, Amidohydro_1, 1 hit PF02786, CPSase_L_D2, 2 hits PF02787, CPSase_L_D3, 1 hit PF00988, CPSase_sm_chain, 1 hit PF00117, GATase, 1 hit PF02142, MGS, 1 hit PF00185, OTCace, 1 hit PF02729, OTCace_N, 1 hit |
PRINTSi | PR00100, AOTCASE PR00098, CPSASE |
SMARTi | View protein in SMART SM01096, CPSase_L_D3, 1 hit SM01097, CPSase_sm_chain, 1 hit SM00851, MGS, 1 hit |
SUPFAMi | SSF48108, SSF48108, 1 hit SSF51338, SSF51338, 1 hit SSF51556, SSF51556, 1 hit SSF52021, SSF52021, 1 hit SSF52317, SSF52317, 1 hit SSF52335, SSF52335, 1 hit SSF52440, SSF52440, 2 hits SSF53671, SSF53671, 1 hit |
TIGRFAMsi | TIGR00670, asp_carb_tr, 1 hit TIGR01369, CPSaseII_lrg, 1 hit TIGR01368, CPSaseIIsmall, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 2 hits PS00097, CARBAMOYLTRANSFERASE, 1 hit PS00866, CPSASE_1, 2 hits PS00867, CPSASE_2, 2 hits PS00482, DIHYDROOROTASE_1, 1 hit PS00483, DIHYDROOROTASE_2, 1 hit PS51273, GATASE_TYPE_1, 1 hit PS51855, MGS, 1 hit |
i Sequence
Sequence statusi: Complete.
Q91437-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATLFLDDGS SFKGRLFGAS STVSGEVVFQ TGMVGYPEAL TDPSYLSQIL
60 70 80 90 100
VLTYPLIGNY GIPKDEEDEH GLSKWFESAK IHAAALVIGE NSQNPSHWSS
110 120 130 140 150
VRSLDQRLKE HGIPALEGID TRSLTKKIRE KGTLLGKLVI DGTDENSLPY
160 170 180 190 200
DDPNKRHLVK EVSIKEPKVY HPSGNVKIMA VDCGMKYNQI RSLCKRGAAV
210 220 230 240 250
TVVPWDYLFD SNEFDGLFIS NGPGDPEYCQ QTINNVKKAI SEEKPKPLFG
260 270 280 290 300
ICLGHQILSL AIGAKTYKMK YGNRGHNQPC IHEGTQRCFY TSQNHGFAVE
310 320 330 340 350
PCSLPRDWSV LFTNANDQSN EGIIHNSKPL FSVQFHPEHK AGPTDLVDLF
360 370 380 390 400
DIFLECARDV KLGVNLDKTV KGRVISHYSF KNGTENSKTP PGRIQPHKVL
410 420 430 440 450
ILGSGGLSIG QAGEFDYSGS QAIKALKEEN VQSVLINPNI ATVQTSKGLA
460 470 480 490 500
DKVYFLPITP EYVTQVIMNE RPDGILLTFG GQTALNCGVE LQKRGVLEKY
510 520 530 540 550
HVRVLGTPVS SIEMTEDRKI FVEKMAEINE YVVPSEAAFT LEQAQGAAER
560 570 580 590 600
LGYPVLVRAA FALGGLGSGF AQNKEELVTL VTQAFAHTSQ ILVDKSLKGW
610 620 630 640 650
KEIEYEVVRD AYDNCITVCN MENVDPLGIH TGESIVVAPS QTLNDKEYNL
660 670 680 690 700
LRTTAIKVIR HLGVVGECNI QYALSPESEQ YFIIEVNARL SRSSALASKA
710 720 730 740 750
TGYPLAYVAA KLALGIPLPV LRNSVTNSTT ANYEPSLDYC VVKVPRWDLS
760 770 780 790 800
KFLRLSTKIG SSMKSVGEVM AIGRNFEEAF QKALRMVDEN CVGFDHTLKP
810 820 830 840 850
ASDEELETPT DKRIFVLAAA LRAGYEIDRL YELTKIDKWF LHKMKNIVEY
860 870 880 890 900
SLKLSELYMK DEVPRHDLLK VKRLGFSDKQ IAMAIQSTEL AVRRLRQEWK
910 920 930 940 950
ILPVVKQIDT VAAEWPAQTN YLYLTYNGEG HDLDFTKPHV MVIGSGVYRI
960 970 980 990 1000
GSSVEFDWCA VRCIQQLRKM GYKTRMVNYN PETVSTDYDM CDRLYFDEIS
1010 1020 1030 1040 1050
FEVVMDIYEL ENPEGIILSM GGQLPNNIAM DLHRQQCRIL GTSPESIDTA
1060 1070 1080 1090 1100
ENRFKFSRML DTIGISQPRW KELSDTESSK QFCTKVGYPC LIRPSYVLSG
1110 1120 1130 1140 1150
VAMNVAYSDN DLEKFLSSAV AVSKEHPVVI SKFIQEAKEI DVDAVACDGV
1160 1170 1180 1190 1200
VIAVAISEHV ENAGVHSGDA TLVTPPQDLN QKTTERIKAI VHAIGQELQA
1210 1220 1230 1240 1250
TGPFNLQLIA KDDQLKVIEC NVRVSRSFPF VSKTLGVDMI ALATKVIMGE
1260 1270 1280 1290 1300
EVEPVGLMTG TGVVGVKVPQ FSFSRLAGAD VVLGVEMTST GEVACFGENR
1310 1320 1330 1340 1350
YEAYLKAMLS TGFKIPKKNI LLSIGSYKNK SELLSTVQSL EQLGYNLYAS
1360 1370 1380 1390 1400
LGTADFYTEH GVKIKAVDWP FEDTDNGCPL KERHRNIMDY LEENHFDLVI
1410 1420 1430 1440 1450
NLSMRNSGGR RLSSFVTKGY RTRRLAVDYS VPLIIDIKCT KLFVEALRLV
1460 1470 1480 1490 1500
GDTPPVKTHI DSMSSHKLIR LPGLIDVHVH LREPGGTHKE DFASGTAAAL
1510 1520 1530 1540 1550
AGGVTMVCAM PNTNPAITDQ TSFALVQKLA TAGARCDFAL FLGASSDNAD
1560 1570 1580 1590 1600
VLPLISNSAA GLKMYLNDTF STLKMDNVSL WMEHFEKWPK HLPIVVHAER
1610 1620 1630 1640 1650
QTVAAILMVA QLYQRPVHIC HVARKEEIQI IRAAKQKGVQ VTCEVAPHHL
1660 1670 1680 1690 1700
FLNEEDLESI GHGKGQVRPM LSTKEDVNAL WENLDVIDCF ATDHAPHSVE
1710 1720 1730 1740 1750
EKNSDSPPPG YPGLETMLPL LLTAVSEGRL TIDDLVKRLY ENPRKIFSLP
1760 1770 1780 1790 1800
VQENTYVEVD LEQEWIIPSY MQFTKSKWTP FEGKKVKGRV RRVVLRGEVA
1810 1820 1830 1840 1850
YIDGQVLVPP GYGQDVRAWP LGVPLPPPPT TVKTPEHSKP TQTETVRTRT
1860 1870 1880 1890 1900
ASPRRLASSG PAVDARFHLP PRIHRCSDPG LPNAEGEYKE KPVKKFIEQD
1910 1920 1930 1940 1950
TVSQDGYIYP PPVSRLLSPQ NLAAQAVPHP YSLLLHPFVG QHILSVKRFT
1960 1970 1980 1990 2000
KDQLSHLFNV AHNLRLTVQK DRSLDILKGK VMASMFYEVS TRTSSSFRAA
2010 2020 2030 2040 2050
MHRLGGSVIH FSEATSSVQK GESLLDSVQT MSCYVDVVVL RHPEPGAVEL
2060 2070 2080 2090 2100
AAKHSRKPII NAGDGVGEHP TQALLDIFTI REELGTVNGM TITMVGDLKH
2110 2120 2130 2140 2150
GRTVHSLAYL LTLYRVNLRY VTPRNLRMPP NIIRFLASRG IKQEEFDSLE
2160 2170 2180 2190 2200
EALPDTDVLY MTRIQKERFA SEEEYEACFG QFILTPHIMT KGKKKMVVMH
2210 2220 2230 2240
PLPRVNEVSV EVDSDPRAAY FRQAENGMYV RMALLATVLG KF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18868 mRNA Translation: AAA74569.1 |
PIRi | A57541 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18868 mRNA Translation: AAA74569.1 |
PIRi | A57541 |
3D structure databases
SMRi | Q91437 |
ModBasei | Search... |
Enzyme and pathway databases
UniPathwayi | UPA00070;UER00115 UPA00070;UER00116 UPA00070;UER00117 |
Family and domain databases
CDDi | cd01744, GATase1_CPSase, 1 hit |
Gene3Di | 1.10.1030.10, 1 hit 3.30.1490.20, 1 hit 3.40.50.1370, 2 hits 3.40.50.1380, 1 hit 3.40.50.880, 1 hit 3.50.30.20, 1 hit |
HAMAPi | MF_00001, Asp_carb_tr, 1 hit MF_01209, CPSase_S_chain, 1 hit |
InterProi | View protein in InterPro IPR006680, Amidohydro-rel IPR006132, Asp/Orn_carbamoyltranf_P-bd IPR006130, Asp/Orn_carbamoylTrfase IPR036901, Asp/Orn_carbamoylTrfase_sf IPR002082, Asp_carbamoyltransf IPR006131, Asp_carbamoyltransf_Asp/Orn-bd IPR011761, ATP-grasp IPR013815, ATP_grasp_subdomain_1 IPR006275, CarbamoylP_synth_lsu IPR005480, CarbamoylP_synth_lsu_oligo IPR036897, CarbamoylP_synth_lsu_oligo_sf IPR006274, CarbamoylP_synth_ssu IPR002474, CarbamoylP_synth_ssu_N IPR036480, CarbP_synth_ssu_N_sf IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR005483, CbamoylP_synth_lsu_CPSase_dom IPR029062, Class_I_gatase-like IPR035686, CPSase_GATase1 IPR002195, Dihydroorotase_CS IPR017926, GATASE IPR011059, Metal-dep_hydrolase_composite IPR032466, Metal_Hydrolase IPR011607, MGS-like_dom IPR036914, MGS-like_dom_sf IPR016185, PreATP-grasp_dom_sf |
Pfami | View protein in Pfam PF01979, Amidohydro_1, 1 hit PF02786, CPSase_L_D2, 2 hits PF02787, CPSase_L_D3, 1 hit PF00988, CPSase_sm_chain, 1 hit PF00117, GATase, 1 hit PF02142, MGS, 1 hit PF00185, OTCace, 1 hit PF02729, OTCace_N, 1 hit |
PRINTSi | PR00100, AOTCASE PR00098, CPSASE |
SMARTi | View protein in SMART SM01096, CPSase_L_D3, 1 hit SM01097, CPSase_sm_chain, 1 hit SM00851, MGS, 1 hit |
SUPFAMi | SSF48108, SSF48108, 1 hit SSF51338, SSF51338, 1 hit SSF51556, SSF51556, 1 hit SSF52021, SSF52021, 1 hit SSF52317, SSF52317, 1 hit SSF52335, SSF52335, 1 hit SSF52440, SSF52440, 2 hits SSF53671, SSF53671, 1 hit |
TIGRFAMsi | TIGR00670, asp_carb_tr, 1 hit TIGR01369, CPSaseII_lrg, 1 hit TIGR01368, CPSaseIIsmall, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 2 hits PS00097, CARBAMOYLTRANSFERASE, 1 hit PS00866, CPSASE_1, 2 hits PS00867, CPSASE_2, 2 hits PS00482, DIHYDROOROTASE_1, 1 hit PS00483, DIHYDROOROTASE_2, 1 hit PS51273, GATASE_TYPE_1, 1 hit PS51855, MGS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PYR1_SQUAC | |
Accessioni | Q91437Primary (citable) accession number: Q91437 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families