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Protein

F-box only protein 5-A

Gene

fbxo5-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates progression through early mitosis by inhibiting the anaphase promoting complex/cyclosome (APC) (PubMed:15314241). Binds to the APC activator cdc20 to prevent APC activation (PubMed:11389834). Can also bind directly to the APC to inhibit substrate-binding (PubMed:11751633). Required to arrest unfertilized eggs at metaphase of meiosis II, by preventing their release from metaphase of meiosis II, through inhibition of APC-dependent cyclin B destruction leading to stabilization of cyclin B-cdk1 complex activity (PubMed:11976684).4 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri319 – 367ZBR-typePROSITE-ProRule annotationAdd BLAST49

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 5-ACurated
Alternative name(s):
Early mitotic inhibitor 1-A
Gene namesi
Name:fbxo5-a
Synonyms:emi1-a
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-953951 fbxo5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi10S → A: Not mitotically degraded; when associated with A-29; A-105; A-123 and A-328. Does not affect fbxo5 proteolysis. Impairs pin1 interaction. Impairs pin1 interaction; when associated with A-29. Impairs pin1 interaction; when associated with A-29 and A-105. Impairs pin1 interaction; when associated with A-29; A-105 and A-123. Impairs pin1 interaction; when associated with A-29; A-105; A-123 and A-328. Proteolysated in the presence of Pin1. Degraded at G2 phase. 2 Publications1
Mutagenesisi29S → A: Not mitotically degraded; when associated with A-10; A-105; A-123 and A-328. Delays fbxo5 proteolysis. Does not affect pin1 interaction. Impairs pin1 interaction; when associated with A-10. Impairs pin1 interaction; when associated with A-10 and A-105. Impairs pin1 interaction; when associated with A-10; A-105 and A-123. Impairs pin1 interaction; when associated with A-10; A-105; A-123 and A-328. 2 Publications1
Mutagenesisi95S → A: Does not affect protein expression in meiosis II; when associated with A-99. Does not affect protein expression in entered M phase; when associated with A-99. Mostly inhibits the degradation of cyclin B at the meiosis I exit, maintains cdk1 activity at a high level, and arrests meiosis I at metaphase; when associated with A-99. 1 Publication1
Mutagenesisi99S → A: Does not affect protein expression in meiosis II; when associated with A-95. Does not affect protein expression in entered M phase; when associated with A-95. Mostly inhibits the degradation of cyclin B at the meiosis I exit, maintains cdk1 activity at a high level, and arrests meiosis I at metaphase; when associated with A-95. 1 Publication1
Mutagenesisi105S → A: Not mitotically degraded; when associated with A-10; A-29; A-123 and A-328. Does not affect fbxo5 proteolysis. Does not affect pin1 interaction. Impairs pin1 interaction; when associated with A-10 and A-29. Impairs pin1 interaction; when associated with A-10; A-29 and A-123. Impairs pin1 interaction; when associated with A-10; A-29; A-123 and A-328. 2 Publications1
Mutagenesisi123T → A: Not mitotically degraded; when associated with A-10; A-29; A-105 and A-328. Delays fbxo5 proteolysis. Does not affect pin1 interaction. Impairs pin1 interaction; when associated with A-10; A-29 and A-105. Impairs pin1 interaction; when associated with A-10; A-29; A-105 and A-328. 2 Publications1
Mutagenesisi198 – 199EL → AA: Abolishes binding to skp1. 1 Publication2
Mutagenesisi328S → A: Not mitotically degraded; when associated with A-10; A-29; A-105 and A-123. Does not affect fbxo5 proteolysis. Does not affect pin1 interaction. Impairs pin1 interaction; when associated with A-10; A-29; A-105 and A-123. 2 Publications1
Mutagenesisi341C → S: Does not inhibit APC. 1 Publication1
Mutagenesisi346C → S: Does not inhibit APC. 1 Publication1
Mutagenesisi351C → S: Does not inhibit APC. 1 Publication1
Mutagenesisi354C → S: Does not inhibit APC; when associated with S-356. 1 Publication1
Mutagenesisi356C → S: Does not inhibit APC; when associated with S-354. 1 Publication1
Mutagenesisi364C → S: Does not inhibit APC. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002580101 – 392F-box only protein 5-AAdd BLAST392

Post-translational modificationi

Proteolysed; proteolysis is induced by both cyclin B-cdk1 and cyclin A-cdk1/2 complex through probable phosphorylation. Proteolysis is inhibited by pin1 during G2.1 Publication

Expressioni

Developmental stagei

Accumulates before mitosis with levels increasing in S phase and decreasing in M phase (PubMed:11389834). Weakly detected in cycling egg at the stage just before entry into M phase, but not detected in immature (stage VI), metaphase-I and metaphase-II oocytes, and cycling egg that has entered in M phase. Destroyed at every M phase during both meiotic cycles and during cleavage cycles (PubMed:15314241). Decreases at metaphase and completly disappears as cells exited mitosis. First observed at 4 h (S phase) reaching a peak at 7 h (G2 phase), and then a subsequent decrease from 9 to 10 h corresponding to early and late M phases, respectively (PubMed:17159919).3 Publications

Inductioni

Expression is first activated after the gastrula stage of development.1 Publication

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By similarity). Interacts with btrc (PubMed:17159919). Interacts with skp1 (PubMed:11389834). Interacts with cdc20 (PubMed:11976684, PubMed:11389834). Interacts with pin1; stabilizes fbxo5 by preventing its association with btrc in an isomerization-dependent pathway; this interaction is present during G2 phase and prevents fbxo5 degradation (PubMed:17159919). Interacts with plk1 (PubMed:17159919).By similarity3 Publications

Binary interactionsi

Protein-protein interaction databases

ELMiQ90Z80
IntActiQ90Z80, 3 interactors
MINTiQ90Z80

Structurei

3D structure databases

SMRiQ90Z80
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini197 – 244F-boxSequence analysisAdd BLAST48

Domaini

The C-terminal region is required for APC inhibition.1 Publication

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri319 – 367ZBR-typePROSITE-ProRule annotationAdd BLAST49

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOVERGENiHBG010089
KOiK10292

Family and domain databases

InterProiView protein in InterPro
IPR036047 F-box-like_dom_sf
IPR001810 F-box_dom
PfamiView protein in Pfam
PF00646 F-box, 1 hit
SUPFAMiSSF81383 SSF81383, 1 hit
PROSITEiView protein in PROSITE
PS51872 ZF_ZBR, 1 hit

Sequencei

Sequence statusi: Complete.

Q90Z80-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMCGFASNQS PKKLSSKKSS ATNVHLEISP VKHHPPCKVY ENVQGSCLDS
60 70 80 90 100
AICTTVAKCA DLTDDLPVHN KENLLHRLND LETNSYEEYS ALQDSGYSSI
110 120 130 140 150
LQNDSPCQDE TDRKVSDIQV RETPKNFMSY QRPFHTLSKI NLPILRFEEA
160 170 180 190 200
VCSTLKKMRK TNKKIDWNAV DVVCGGNYGL EHLIGKSMGL ERFDILAELF
210 220 230 240 250
HRDFKHLLTK ILRHLSAMDL INVISVSTTW RKLLQKDNWA YNAYKLGCKE
260 270 280 290 300
LCEKRAKVSS HTATRDESLC RVPLASVQKV AASSLCTSKK QSKNKNGGLS
310 320 330 340 350
CNRLAEFIEV AQTLKNDQSL KVCVDCGSPA KHDPCLHRAI CTRESCKLDF
360 370 380 390
CTRCSCKYHF SKSCLMSKPG SYRIPSEPLP GSKKSKQNLR RL
Length:392
Mass (Da):44,142
Last modified:December 1, 2001 - v1
Checksum:i06E6627033263612
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF319594 mRNA Translation: AAK62272.1
BC088910 mRNA Translation: AAH88910.1
RefSeqiNP_001082122.1, NM_001088653.1
UniGeneiXl.11644

Genome annotation databases

GeneIDi398237
KEGGixla:398237

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF319594 mRNA Translation: AAK62272.1
BC088910 mRNA Translation: AAH88910.1
RefSeqiNP_001082122.1, NM_001088653.1
UniGeneiXl.11644

3D structure databases

SMRiQ90Z80
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ELMiQ90Z80
IntActiQ90Z80, 3 interactors
MINTiQ90Z80

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi398237
KEGGixla:398237

Organism-specific databases

CTDi398237
XenbaseiXB-GENE-953951 fbxo5

Phylogenomic databases

HOVERGENiHBG010089
KOiK10292

Enzyme and pathway databases

UniPathwayi
UPA00143

Family and domain databases

InterProiView protein in InterPro
IPR036047 F-box-like_dom_sf
IPR001810 F-box_dom
PfamiView protein in Pfam
PF00646 F-box, 1 hit
SUPFAMiSSF81383 SSF81383, 1 hit
PROSITEiView protein in PROSITE
PS51872 ZF_ZBR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFBX5A_XENLA
AccessioniPrimary (citable) accession number: Q90Z80
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: December 1, 2001
Last modified: November 7, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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