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Protein

G-protein coupled receptor 161

Gene

gpr161

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Key negative regulator of Shh signaling during neural tube development. Recruited to primary cilia and acts as a regulator of the PKA-dependent basal repression machinery in Shh signaling by increasing cAMP levels, leading to promote the PKA-dependent processing of gli3 into gli3r and repress the Shh signaling. In presence of shh, it is removed from primary cilia, preventing its activity and allowing activation of the Shh signaling (By similarity). Required in left/right patterning by modulating Ca2+ levels in the cells surrounding the Kupffer vesicle.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-DRE-5610787 Hedgehog 'off' state
R-DRE-5632684 Hedgehog 'on' state

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor 161
Gene namesi
Name:gpr161
ORF Names:si:rp71-20i5.4
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 9

Organism-specific databases

ZFINiZDB-GENE-030616-58 gpr161

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 27ExtracellularSequence analysisAdd BLAST27
Transmembranei28 – 48Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini49 – 60CytoplasmicSequence analysisAdd BLAST12
Transmembranei61 – 81Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini82 – 98ExtracellularSequence analysisAdd BLAST17
Transmembranei99 – 119Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini120 – 139CytoplasmicSequence analysisAdd BLAST20
Transmembranei140 – 160Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini161 – 186ExtracellularSequence analysisAdd BLAST26
Transmembranei187 – 207Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini208 – 263CytoplasmicSequence analysisAdd BLAST56
Transmembranei264 – 284Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini285 – 300ExtracellularSequence analysisAdd BLAST16
Transmembranei301 – 321Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini322 – 526CytoplasmicSequence analysisAdd BLAST205

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Membrane

Pathology & Biotechi

Disruption phenotypei

Embryos exhibit perturb cardiac looping as the result of a defect in left/right patterning.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003790741 – 526G-protein coupled receptor 161Add BLAST526

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi2N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi6N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi12N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi96 ↔ 174PROSITE-ProRule annotation
Glycosylationi97N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ90X46

Expressioni

Developmental stagei

Expressed throughout embryonic development. At 9- to 16-somites, transcripts are broadly expressed with specific staining observed in the developing nervous system, somites, and precardiac mesoderm. At later stages 1- to 3-days post-fertilization, transcripts become more localized within the dorsal diencephalon, the otic vesicles, and the fin buds.

Gene expression databases

BgeeiENSDARG00000055659 Expressed in 27 organ(s), highest expression level in multi-cellular organism

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000118097

Structurei

3D structure databases

ProteinModelPortaliQ90X46
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi30 – 35Poly-Ile6
Compositional biasi512 – 516Poly-Asp5

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00920000149036
HOGENOMiHOG000112777
InParanoidiQ90X46
KOiK08439
OMAiIWCALLP
OrthoDBiEOG091G0G56
PhylomeDBiQ90X46
TreeFamiTF331895

Family and domain databases

InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q90X46-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNGSKNGTAV ANSTNGLDDN GLMVLESVSI IIIAILACLG NLVIVVTLYK
60 70 80 90 100
KPYLLTPSNK FVFSLTSSNL LLSVLMLPFV VASSVRRDWM FGVVWCNFTA
110 120 130 140 150
LLHLLVSSSS MLTLGAIAID RYYAVLYPMI YPMKITGNRA VLAIVYIWLH
160 170 180 190 200
SLVGCLPPLF GWSSFEFDRF KWTCTVSWHK EISYTAFWVT WCCLLPLVAM
210 220 230 240 250
LVCYGVIFRV ARIKARKVYC GSVVVSQEES SSQNNGRKNS NTSTSSSGSR
260 270 280 290 300
KSLIYSGSQC KAFITILVVL GTFLTTWGPY VVVISTEALL GKNSVSPQVE
310 320 330 340 350
TLVSWLSFTS AVCHPLIYGL WNKTVRKELL GMCFDDRYYR ESFVIRHRTS
360 370 380 390 400
RLFSISNRIT DLGMSPHLTA MLVGGGQLLG RGSSTGDTGF SYTQDSATDV
410 420 430 440 450
MLLESYTSEA SHSAHCTANK RRSSVTFEDQ VDHIPQGDPS VVQVTADIHK
460 470 480 490 500
SLDSFASSLA KAIENDAKLQ LLGEWTQIPT SLFTVRNTQR VPRYLDGQRL
510 520
RMESIDEGIV KDDDDDEEEM EREEKM
Length:526
Mass (Da):58,485
Last modified:March 1, 2003 - v2
Checksum:iCACE795D638B5B3E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti84S → T in AAI35012 (Ref. 3) Curated1
Sequence conflicti519E → EE in AAI35012 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU090912 mRNA Translation: ABW77593.1
AL590146 Genomic DNA Translation: CAC94897.2
BC135011 mRNA Translation: AAI35012.1
RefSeqiNP_001007200.2, NM_001007199.2
UniGeneiDr.88054

Genome annotation databases

EnsembliENSDART00000078051; ENSDARP00000072514; ENSDARG00000055659
ENSDART00000143991; ENSDARP00000118097; ENSDARG00000055659
GeneIDi368421
KEGGidre:368421

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU090912 mRNA Translation: ABW77593.1
AL590146 Genomic DNA Translation: CAC94897.2
BC135011 mRNA Translation: AAI35012.1
RefSeqiNP_001007200.2, NM_001007199.2
UniGeneiDr.88054

3D structure databases

ProteinModelPortaliQ90X46
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000118097

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PaxDbiQ90X46

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000078051; ENSDARP00000072514; ENSDARG00000055659
ENSDART00000143991; ENSDARP00000118097; ENSDARG00000055659
GeneIDi368421
KEGGidre:368421

Organism-specific databases

CTDi23432
ZFINiZDB-GENE-030616-58 gpr161

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00920000149036
HOGENOMiHOG000112777
InParanoidiQ90X46
KOiK08439
OMAiIWCALLP
OrthoDBiEOG091G0G56
PhylomeDBiQ90X46
TreeFamiTF331895

Enzyme and pathway databases

ReactomeiR-DRE-5610787 Hedgehog 'off' state
R-DRE-5632684 Hedgehog 'on' state

Miscellaneous databases

PROiPR:Q90X46

Gene expression databases

BgeeiENSDARG00000055659 Expressed in 27 organ(s), highest expression level in multi-cellular organism

Family and domain databases

InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGP161_DANRE
AccessioniPrimary (citable) accession number: Q90X46
Secondary accession number(s): A4IGB3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: March 1, 2003
Last modified: September 12, 2018
This is version 99 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
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