UniProtKB - Q90617 (LAMP2_CHICK)
Lysosome-associated membrane glycoprotein 2
LAMP2
Functioni
Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live. Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation (By similarity).
Plays a role in lysosomal protein degradation in response to starvation (By similarity).
Required for the fusion of autophagosomes with lysosomes during autophagy. Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes. Required for normal degradation of the contents of autophagosomes. Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits. Is not required for efficient MHCII-mediated presentation of endogenous antigens (By similarity).
By similarityGO - Biological processi
- autophagosome maturation Source: UniProtKB
- cellular response to starvation Source: UniProtKB
- chaperone-mediated autophagy Source: UniProtKB
- establishment of protein localization to organelle Source: GO_Central
- lysosomal protein catabolic process Source: UniProtKB
- protein targeting Source: UniProtKB
- protein targeting to lysosome involved in chaperone-mediated autophagy Source: UniProtKB
Keywordsi
Biological process | Autophagy |
Names & Taxonomyi
Protein namesi | Recommended name: Lysosome-associated membrane glycoprotein 2Short name: LAMP-2 Short name: Lysosome-associated membrane protein 2 |
Gene namesi | Name:LAMP2 |
Organismi | Gallus gallus (Chicken) |
Taxonomic identifieri | 9031 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archelosauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galloanserae › Galliformes › Phasianidae › Phasianinae › Gallus |
Proteomesi |
|
Subcellular locationi
Endosome
- Endosome membrane By similarity; Single-pass type I membrane protein PROSITE-ProRule annotation
Lysosome
- Lysosome membrane PROSITE-ProRule annotation; Single-pass type I membrane protein PROSITE-ProRule annotation
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein PROSITE-ProRule annotation
Other locations
- autophagosome membrane By similarity
Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane.By similarity
Endosome
- late endosome membrane Source: GO_Central
Lysosome
- lysosomal membrane Source: GO_Central
- lysosome Source: UniProtKB
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- integral component of autophagosome membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 28 – 389 | LumenalSequence analysisAdd BLAST | 362 | |
Transmembranei | 390 – 414 | HelicalPROSITE-ProRule annotationAdd BLAST | 25 | |
Topological domaini | 415 – 425 | CytoplasmicPROSITE-ProRule annotationAdd BLAST | 11 |
Keywords - Cellular componenti
Cell membrane, Cytoplasmic vesicle, Endosome, Lysosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | Sequence analysisAdd BLAST | 27 | |
ChainiPRO_0000017114 | 28 – 425 | Lysosome-associated membrane glycoprotein 2Add BLAST | 398 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 37 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 40 ↔ 78 | PROSITE-ProRule annotation | ||
Glycosylationi | 56 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 62 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 74 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 100 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 105 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 120 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 153 ↔ 189 | PROSITE-ProRule annotation | ||
Glycosylationi | 163 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 170 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 179 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 206 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 232 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 239 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 242 ↔ 274 | PROSITE-ProRule annotation | ||
Glycosylationi | 252 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 276 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 287 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 298 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 312 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 320 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 331 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 345 ↔ 382 | PROSITE-ProRule annotation |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q90617 |
Expressioni
Gene expression databases
Bgeei | ENSGALG00000008572, Expressed in spermatocyte and 14 other tissues |
Interactioni
Subunit structurei
Monomer. Forms large homooligomers.
Interacts (via its cytoplasmic region) with HSPA8.
Interacts with HSP90 in the lysosome lumen; this enhances LAMP2 stability (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 9031.ENSGALP00000013946 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 28 – 192 | First lumenal domainAdd BLAST | 165 | |
Regioni | 193 – 238 | HingeAdd BLAST | 46 | |
Regioni | 239 – 390 | Second lumenal domainAdd BLAST | 152 | |
Regioni | 416 – 419 | Important for binding and subsequent lysosomal degradation of target proteinsBy similarity | 4 |
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4818, Eukaryota |
HOGENOMi | CLU_055379_2_0_1 |
InParanoidi | Q90617 |
OrthoDBi | 1042920at2759 |
PhylomeDBi | Q90617 |
TreeFami | TF316339 |
Family and domain databases
InterProi | View protein in InterPro IPR018134, LAMP_CS IPR002000, Lysosome-assoc_membr_glycop |
PANTHERi | PTHR11506, PTHR11506, 1 hit |
Pfami | View protein in Pfam PF01299, Lamp, 1 hit |
PRINTSi | PR00336, LYSASSOCTDMP |
PROSITEi | View protein in PROSITE PS00310, LAMP_1, 1 hit PS51407, LAMP_3, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAPPRCPAGL ALLLLLLGAC GFFQSYAVEV DVKDASNFTC LYAQWMMKFL
60 70 80 90 100
IKYETNSSDY KNASLDLTST VTHNGSICGS DTQAALLAVQ FGDGHSWSIN
110 120 130 140 150
FTKNNETYRA EFITFTYNTN DTAVFPDARR QGPVTIVVKD AMHPIQLNNV
160 170 180 190 200
FVCHHTTSLE AENVTQIFWN VTMQPFVQNG TISKKESRCY ADTPTAAPTV
210 220 230 240 250
LPTVANVTTA STTISPAPTT APKPAENPVT GNYSLKTGNK TCLLATVGLQ
260 270 280 290 300
LNISQDKPLL INIDPKTTHA DGTCGNTSAT LKLNDGNRTL IDFTFIVNAS
310 320 330 340 350
ASVQKFYLRE VNVTLLNYQN GSVILSADNN NLSKWDASLG NSYMCRKEQT
360 370 380 390 400
LEINENLQVH TFNLWVQPFL VKENKFSIAE ECFADSDLNF LIPVAVGMAL
410 420
GFLIILVFIS YIIGRRKSRT GYQSV
The sequence of this isoform differs from the canonical sequence as follows:
381-425: ECFADSDLNF...RKSRTGYQSV → DCSPEVDYFI...KHHNTGYEQF
The sequence of this isoform differs from the canonical sequence as follows:
380-425: EECFADSDLN...RKSRTGYQSV → QECSLDDDTI...RKSYAGYQTL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 261 | I → L in AAA99539 (PubMed:7546292).Curated | 1 | |
Sequence conflicti | 261 | I → L in AAA99541 (PubMed:7546292).Curated | 1 | |
Sequence conflicti | 277 | T → A in AAA99539 (PubMed:7546292).Curated | 1 | |
Sequence conflicti | 277 | T → A in AAA99541 (PubMed:7546292).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_003047 | 380 – 425 | EECFA…GYQSV → QECSLDDDTILIPIVVGAAL AGLIVIIVIAYIIGRRKSYA GYQTL in isoform LAMP-2C. 1 PublicationAdd BLAST | 46 | |
Alternative sequenceiVSP_003048 | 381 – 425 | ECFAD…GYQSV → DCSPEVDYFIVPIAVGAALG GLVVLVIMAYFLGHKKHHNT GYEQF in isoform LAMP-2A. 1 PublicationAdd BLAST | 45 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U10547 mRNA Translation: AAA99540.1 U10546 mRNA Translation: AAA99539.1 U10548 mRNA Translation: AAA99541.1 |
RefSeqi | NP_001001749.1, NM_001001749.1 [Q90617-1] |
Genome annotation databases
GeneIDi | 414800 |
KEGGi | gga:414800 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U10547 mRNA Translation: AAA99540.1 U10546 mRNA Translation: AAA99539.1 U10548 mRNA Translation: AAA99541.1 |
RefSeqi | NP_001001749.1, NM_001001749.1 [Q90617-1] |
3D structure databases
SMRi | Q90617 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9031.ENSGALP00000013946 |
Proteomic databases
PaxDbi | Q90617 |
Genome annotation databases
GeneIDi | 414800 |
KEGGi | gga:414800 |
Organism-specific databases
CTDi | 3920 |
Phylogenomic databases
eggNOGi | KOG4818, Eukaryota |
HOGENOMi | CLU_055379_2_0_1 |
InParanoidi | Q90617 |
OrthoDBi | 1042920at2759 |
PhylomeDBi | Q90617 |
TreeFami | TF316339 |
Miscellaneous databases
PROi | PR:Q90617 |
Gene expression databases
Bgeei | ENSGALG00000008572, Expressed in spermatocyte and 14 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR018134, LAMP_CS IPR002000, Lysosome-assoc_membr_glycop |
PANTHERi | PTHR11506, PTHR11506, 1 hit |
Pfami | View protein in Pfam PF01299, Lamp, 1 hit |
PRINTSi | PR00336, LYSASSOCTDMP |
PROSITEi | View protein in PROSITE PS00310, LAMP_1, 1 hit PS51407, LAMP_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LAMP2_CHICK | |
Accessioni | Q90617Primary (citable) accession number: Q90617 Secondary accession number(s): Q90616, Q90618 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | September 29, 2021 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families