UniProtKB - Q90611 (MMP2_CHICK)
Protein
72 kDa type IV collagenase
Gene
MMP2
Organism
Gallus gallus (Chicken)
Status
Functioni
Catalytic activityi
- Cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln.By similarity EC:3.4.24.24
Cofactori
Protein has several cofactor binding sites:- Ca2+By similarityNote: Binds 4 Ca2+ ions per subunit.By similarity
- Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 99 | Zinc 1; in inhibited formBy similarity | 1 | |
Metal bindingi | 131 | Calcium 1By similarity | 1 | |
Metal bindingi | 165 | Calcium 2; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 175 | Zinc 2By similarity | 1 | |
Metal bindingi | 177 | Zinc 2By similarity | 1 | |
Metal bindingi | 182 | Calcium 3By similarity | 1 | |
Metal bindingi | 183 | Calcium 3; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 190 | Zinc 2By similarity | 1 | |
Metal bindingi | 197 | Calcium 2; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 199 | Calcium 2; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 201 | Calcium 2By similarity | 1 | |
Metal bindingi | 203 | Zinc 2By similarity | 1 | |
Metal bindingi | 205 | Calcium 3By similarity | 1 | |
Metal bindingi | 206 | Calcium 1By similarity | 1 | |
Metal bindingi | 208 | Calcium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 208 | Calcium 3By similarity | 1 | |
Metal bindingi | 400 | Zinc 1; catalyticBy similarity | 1 | |
Active sitei | 401 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 404 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 410 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 479 | Calcium 4; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 524 | Calcium 4; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 572 | Calcium 4; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 621 | Calcium 4; via carbonyl oxygenBy similarity | 1 |
GO - Molecular functioni
- metalloendopeptidase activity Source: AgBase
- zinc ion binding Source: InterPro
GO - Biological processi
- blood vessel maturation Source: Ensembl
- bone trabecula formation Source: Ensembl
- cellular response to amino acid stimulus Source: Ensembl
- cellular response to insulin-like growth factor stimulus Source: AgBase
- cellular response to reactive oxygen species Source: Ensembl
- cellular response to UV-A Source: Ensembl
- collagen catabolic process Source: GO_Central
- endodermal cell differentiation Source: Ensembl
- extracellular matrix organization Source: GO_Central
- face morphogenesis Source: Ensembl
- intramembranous ossification Source: Ensembl
- negative regulation of cartilage condensation Source: AgBase
- negative regulation of cartilage development Source: AgBase
- negative regulation of focal adhesion assembly Source: AgBase
- negative regulation of protein phosphorylation Source: AgBase
- positive regulation of vascular associated smooth muscle cell proliferation Source: Ensembl
- regulation of MAPK cascade Source: AgBase
- response to amyloid-beta Source: Ensembl
- response to hypoxia Source: GO_Central
- tissue remodeling Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Biological process | Collagen degradation |
Ligand | Calcium, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-GGA-1442490, Collagen degradation R-GGA-1474228, Degradation of the extracellular matrix R-GGA-1592389, Activation of Matrix Metalloproteinases R-GGA-3928665, EPH-ephrin mediated repulsion of cells |
Protein family/group databases
MEROPSi | M10.003 |
Names & Taxonomyi
Protein namesi | Recommended name: 72 kDa type IV collagenase (EC:3.4.24.24By similarity)Alternative name(s): 72 kDa gelatinase Gelatinase A Matrix metalloproteinase-2 Short name: MMP-2 |
Gene namesi | Name:MMP2 |
Organismi | Gallus gallus (Chicken) |
Taxonomic identifieri | 9031 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archelosauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galloanserae › Galliformes › Phasianidae › Phasianinae › Gallus |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- extracellular matrix Curated
Extracellular region or secreted
- extracellular space Source: AgBase
Plasma Membrane
- plasma membrane Source: Ensembl
Other locations
- basement membrane Source: AgBase
- collagen-containing extracellular matrix Source: AgBase
- sarcomere Source: Ensembl
Keywords - Cellular componenti
Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | 1 PublicationAdd BLAST | 26 | |
PropeptideiPRO_0000028722 | 27 – 106 | Activation peptide1 PublicationAdd BLAST | 80 | |
ChainiPRO_0000028723 | 107 – 663 | 72 kDa type IV collagenaseAdd BLAST | 557 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 230 ↔ 256 | PROSITE-ProRule annotation | ||
Disulfide bondi | 244 ↔ 271 | PROSITE-ProRule annotation | ||
Disulfide bondi | 288 ↔ 314 | PROSITE-ProRule annotation | ||
Disulfide bondi | 302 ↔ 329 | PROSITE-ProRule annotation | ||
Disulfide bondi | 346 ↔ 372 | PROSITE-ProRule annotation | ||
Disulfide bondi | 360 ↔ 387 | PROSITE-ProRule annotation | ||
Disulfide bondi | 472 ↔ 663 | PROSITE-ProRule annotation |
Post-translational modificationi
The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3).By similarity
Keywords - PTMi
Disulfide bond, ZymogenProteomic databases
PaxDbi | Q90611 |
Expressioni
Tissue specificityi
Produced by normal skin fibroblasts.
Gene expression databases
Bgeei | ENSGALG00000003580, Expressed in colon and 13 other tissues |
Interactioni
Subunit structurei
Ligand for integrin alpha-V/beta-3.
Protein-protein interaction databases
STRINGi | 9031.ENSGALP00000005656 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 225 – 273 | Fibronectin type-II 1PROSITE-ProRule annotationAdd BLAST | 49 | |
Domaini | 283 – 331 | Fibronectin type-II 2PROSITE-ProRule annotationAdd BLAST | 49 | |
Domaini | 341 – 389 | Fibronectin type-II 3PROSITE-ProRule annotationAdd BLAST | 49 | |
Repeati | 475 – 519 | Hemopexin 1Add BLAST | 45 | |
Repeati | 520 – 566 | Hemopexin 2Add BLAST | 47 | |
Repeati | 568 – 616 | Hemopexin 3Add BLAST | 49 | |
Repeati | 617 – 663 | Hemopexin 4Add BLAST | 47 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 107 – 218 | Collagenase-like 1Add BLAST | 112 | |
Regioni | 219 – 393 | Collagen-bindingAdd BLAST | 175 | |
Regioni | 394 – 468 | Collagenase-like 2Add BLAST | 75 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 97 – 104 | Cysteine switchBy similarity | 8 |
Domaini
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Sequence similaritiesi
Belongs to the peptidase M10A family.Curated
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | KOG1565, Eukaryota |
GeneTreei | ENSGT00940000158511 |
HOGENOMi | CLU_015489_6_2_1 |
InParanoidi | Q90611 |
OMAi | MWCATTK |
OrthoDBi | 1075463at2759 |
PhylomeDBi | Q90611 |
TreeFami | TF315428 |
Family and domain databases
CDDi | cd00062, FN2, 3 hits cd00094, HX, 1 hit cd04278, ZnMc_MMP, 1 hit |
Gene3Di | 1.10.101.10, 1 hit 2.10.10.10, 3 hits 2.110.10.10, 1 hit 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR028708, 72kDa_collagenase IPR000562, FN_type2_dom IPR036943, FN_type2_sf IPR000585, Hemopexin-like_dom IPR036375, Hemopexin-like_dom_sf IPR018487, Hemopexin-like_repeat IPR018486, Hemopexin_CS IPR013806, Kringle-like IPR033739, M10A_MMP IPR024079, MetalloPept_cat_dom_sf IPR001818, Pept_M10_metallopeptidase IPR021190, Pept_M10A IPR021158, Pept_M10A_Zn_BS IPR006026, Peptidase_Metallo IPR002477, Peptidoglycan-bd-like IPR036365, PGBD-like_sf IPR036366, PGBDSf |
PANTHERi | PTHR10201:SF29, PTHR10201:SF29, 1 hit |
Pfami | View protein in Pfam PF00040, fn2, 3 hits PF00045, Hemopexin, 3 hits PF00413, Peptidase_M10, 1 hit PF01471, PG_binding_1, 1 hit |
PIRSFi | PIRSF001191, Peptidase_M10A_matrix, 1 hit |
PRINTSi | PR00138, MATRIXIN |
SMARTi | View protein in SMART SM00059, FN2, 3 hits SM00120, HX, 4 hits SM00235, ZnMc, 1 hit |
SUPFAMi | SSF47090, SSF47090, 1 hit SSF50923, SSF50923, 1 hit SSF57440, SSF57440, 3 hits |
PROSITEi | View protein in PROSITE PS00546, CYSTEINE_SWITCH, 1 hit PS00023, FN2_1, 3 hits PS51092, FN2_2, 3 hits PS00024, HEMOPEXIN, 1 hit PS51642, HEMOPEXIN_2, 4 hits PS00142, ZINC_PROTEASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q90611-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKTHSVFGFF FKVLLIQVYL FNKTLAAPSP IIKFPGDSTP KTDKELAVQY
60 70 80 90 100
LNKYYGCPKD NCNLFVLKDT LKKMQKFFGL PETGDLDQNT IETMKKPRCG
110 120 130 140 150
NPDVANYNFF PRKPKWEKNH ITYRIIGYTP DLDPETVDDA FARAFKVWSD
160 170 180 190 200
VTPLRFNRIN DGEADIMINF GRWEHGDGYP FDGKDGLLAH AFAPGPGIGG
210 220 230 240 250
DSHFDDDELW TLGEGQVVRV KYGNADGEYC KFPFWFNGKE YNSCTDAGRN
260 270 280 290 300
DGFLWCSTTK DFDADGKYGF CPHESLFTMG GNGDGQPCKF PFKFQGQSYD
310 320 330 340 350
QCTTEGRTDG YRWCGTTEDY DRDKKYGFCP ETAMSTVGGN SEGAPCVFPF
360 370 380 390 400
IFLGNKYDSC TSAGRNDGKL WCASTSSYDD DRKWGFCPDQ GYSLFLVAAH
410 420 430 440 450
EFGHAMGLEH SEDPGALMAP IYTYTKNFRL SQDDIKGIQE LYEVSPDVEP
460 470 480 490 500
GPGPGPGPGP RPTLGPVTPE LCKHDIVFDG VAQIRGEIFF FKDRFMWRTV
510 520 530 540 550
NPRGKPTGPL LVATFWPDLP EKIDAVYESP QDEKAVFFAG NEYWVYTASN
560 570 580 590 600
LDRGYPKKLT SLGLPPDVQR IDAAFNWGRN KKTYIFSGDR YWKYNEEKKK
610 620 630 640 650
MELATPKFIA DSWNGVPDNL DAVLGLTDSG YTYFFKDQYY LQMEDKSLKI
660
VKIGKISSDW LGC
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 40 | P → Q AA sequence (PubMed:1848240).Curated | 1 | |
Sequence conflicti | 116 | W → T AA sequence (PubMed:1848240).Curated | 1 | |
Sequence conflicti | 122 | T → I AA sequence (PubMed:1848240).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U07775 mRNA Translation: AAA19596.1 |
PIRi | S46492 |
RefSeqi | NP_989751.1, NM_204420.2 |
Genome annotation databases
Ensembli | ENSGALT00000005666; ENSGALP00000005656; ENSGALG00000003580 |
GeneIDi | 386583 |
KEGGi | gga:386583 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U07775 mRNA Translation: AAA19596.1 |
PIRi | S46492 |
RefSeqi | NP_989751.1, NM_204420.2 |
3D structure databases
SMRi | Q90611 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9031.ENSGALP00000005656 |
Protein family/group databases
MEROPSi | M10.003 |
Proteomic databases
PaxDbi | Q90611 |
Genome annotation databases
Ensembli | ENSGALT00000005666; ENSGALP00000005656; ENSGALG00000003580 |
GeneIDi | 386583 |
KEGGi | gga:386583 |
Organism-specific databases
CTDi | 4313 |
Phylogenomic databases
eggNOGi | KOG1565, Eukaryota |
GeneTreei | ENSGT00940000158511 |
HOGENOMi | CLU_015489_6_2_1 |
InParanoidi | Q90611 |
OMAi | MWCATTK |
OrthoDBi | 1075463at2759 |
PhylomeDBi | Q90611 |
TreeFami | TF315428 |
Enzyme and pathway databases
Reactomei | R-GGA-1442490, Collagen degradation R-GGA-1474228, Degradation of the extracellular matrix R-GGA-1592389, Activation of Matrix Metalloproteinases R-GGA-3928665, EPH-ephrin mediated repulsion of cells |
Miscellaneous databases
PROi | PR:Q90611 |
Gene expression databases
Bgeei | ENSGALG00000003580, Expressed in colon and 13 other tissues |
Family and domain databases
CDDi | cd00062, FN2, 3 hits cd00094, HX, 1 hit cd04278, ZnMc_MMP, 1 hit |
Gene3Di | 1.10.101.10, 1 hit 2.10.10.10, 3 hits 2.110.10.10, 1 hit 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR028708, 72kDa_collagenase IPR000562, FN_type2_dom IPR036943, FN_type2_sf IPR000585, Hemopexin-like_dom IPR036375, Hemopexin-like_dom_sf IPR018487, Hemopexin-like_repeat IPR018486, Hemopexin_CS IPR013806, Kringle-like IPR033739, M10A_MMP IPR024079, MetalloPept_cat_dom_sf IPR001818, Pept_M10_metallopeptidase IPR021190, Pept_M10A IPR021158, Pept_M10A_Zn_BS IPR006026, Peptidase_Metallo IPR002477, Peptidoglycan-bd-like IPR036365, PGBD-like_sf IPR036366, PGBDSf |
PANTHERi | PTHR10201:SF29, PTHR10201:SF29, 1 hit |
Pfami | View protein in Pfam PF00040, fn2, 3 hits PF00045, Hemopexin, 3 hits PF00413, Peptidase_M10, 1 hit PF01471, PG_binding_1, 1 hit |
PIRSFi | PIRSF001191, Peptidase_M10A_matrix, 1 hit |
PRINTSi | PR00138, MATRIXIN |
SMARTi | View protein in SMART SM00059, FN2, 3 hits SM00120, HX, 4 hits SM00235, ZnMc, 1 hit |
SUPFAMi | SSF47090, SSF47090, 1 hit SSF50923, SSF50923, 1 hit SSF57440, SSF57440, 3 hits |
PROSITEi | View protein in PROSITE PS00546, CYSTEINE_SWITCH, 1 hit PS00023, FN2_1, 3 hits PS51092, FN2_2, 3 hits PS00024, HEMOPEXIN, 1 hit PS51642, HEMOPEXIN_2, 4 hits PS00142, ZINC_PROTEASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MMP2_CHICK | |
Accessioni | Q90611Primary (citable) accession number: Q90611 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families