UniProtKB - Q900K4 (Q900K4_9HIV1)
Protein Tat
tat
Functioni
Miscellaneous
Caution
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 11 | Essential for Tat translocation through the endosomal membraneUniRule annotation | 1 | |
Metal bindingi | 22 | Zinc 1UniRule annotation | 1 | |
Metal bindingi | 25 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 27 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 30 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 33 | Zinc 1; via pros nitrogenUniRule annotation | 1 | |
Metal bindingi | 34 | Zinc 1UniRule annotation | 1 | |
Metal bindingi | 37 | Zinc 1UniRule annotation | 1 |
GO - Molecular functioni
- actinin binding Source: UniProtKB-UniRule
- cyclin binding Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-UniRule
- protein domain specific binding Source: UniProtKB-UniRule
- RNA-binding transcription regulator activity Source: UniProtKB-UniRule
- trans-activation response element binding Source: UniProtKB-UniRule
GO - Biological processi
- modulation by virus of host chromatin organization Source: UniProtKB-UniRule
- modulation by virus of host PP1 activity Source: UniProtKB-UniRule
- negative regulation of peptidyl-threonine phosphorylation Source: UniProtKB-UniRule
- positive regulation of transcription elongation from RNA polymerase II promoter Source: UniProtKB-UniRule
- positive regulation of viral transcription Source: UniProtKB-UniRule
- suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-UniRule
- transcription, DNA-templated Source: UniProtKB-UniRule
Keywordsi
Molecular function | ActivatorUniRule annotationARBA annotation, RNA-bindingUniRule annotationARBA annotation |
Biological process | ApoptosisUniRule annotation, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Modulation of host chromatin by virusUniRule annotation, Modulation of host PP1 activity by virusUniRule annotation, Transcription, Transcription regulationUniRule annotationARBA annotation, Viral immunoevasion |
Ligand | Metal-bindingUniRule annotation, ZincUniRule annotation |
Names & Taxonomyi
Protein namesi | Recommended name: Protein TatUniRule annotationARBA annotationAlternative name(s): Transactivating regulatory proteinUniRule annotation |
Gene namesi | Name:tatUniRule annotationImported |
Organismi | Human immunodeficiency virus 1Imported |
Taxonomic identifieri | 11676 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Pararnavirae › Artverviricota › Revtraviricetes › Ortervirales › Retroviridae › Orthoretrovirinae › Lentivirus |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Subcellular locationi
- host nucleolus UniRule annotation
- Host cytoplasm UniRule annotation
- Secreted UniRule annotation Note: Probably localizes to both nuclear and nucleolar compartments. Nuclear localization is mediated through the interaction of the nuclear localization signal with importin KPNB1. Secretion occurs through a Golgi-independent pathway. Tat is released from infected cells to the extracellular space where it remains associated to the cell membrane, or is secreted into the cerebrospinal fluid and sera. Extracellular Tat can be endocytosed by surrounding uninfected cells via binding to several receptors depending on the cell type.UniRule annotation
GO - Cellular componenti
- extracellular region Source: UniProtKB-SubCell
- host cell cytoplasm Source: UniProtKB-SubCell
- host cell nucleolus Source: UniProtKB-SubCell
Keywords - Cellular componenti
Host cytoplasmUniRule annotation, Host nucleusUniRule annotationARBA annotation, SecretedUniRule annotationPTM / Processingi
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 28 | N6-acetyllysine; by host PCAFUniRule annotation | 1 | |
Modified residuei | 50 | N6-acetyllysine; by host EP300 and GCN5L2UniRule annotation | 1 | |
Modified residuei | 51 | N6-acetyllysine; by host EP300 and GCN5L2UniRule annotation | 1 | |
Modified residuei | 52 | Asymmetric dimethylarginine; by host PRMT6UniRule annotation | 1 | |
Modified residuei | 53 | Asymmetric dimethylarginine; by host PRMT6UniRule annotation | 1 | |
Cross-linki | 71 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)UniRule annotation |
Post-translational modificationi
Keywords - PTMi
AcetylationUniRule annotation, Isopeptide bondUniRule annotation, MethylationUniRule annotation, PhosphoproteinUniRule annotation, Ubl conjugationUniRule annotationInteractioni
Subunit structurei
Interacts with host CCNT1. Associates with the P-TEFb complex composed at least of Tat, P-TEFb (CDK9 and CCNT1), TAR RNA, RNA Pol II. Recruits the HATs CREBBP, TAF1/TFIID, EP300, PCAF and GCN5L2.
Interacts with host KAT5/Tip60; this interaction targets the latter to degradation.
Interacts with the host deacetylase SIRT1.
Interacts with host capping enzyme RNGTT; this interaction stimulates RNGTT. Binds to host KDR, and to the host integrins ITGAV/ITGB3 and ITGA5/ITGB1.
Interacts with host KPNB1/importin beta-1 without previous binding to KPNA1/importin alpha-1.
Interacts with EIF2AK2.
Interacts with host nucleosome assembly protein NAP1L1; this interaction may be required for the transport of Tat within the nucleus, since the two proteins interact at the nuclear rim.
Interacts with host C1QBP/SF2P32; this interaction involves lysine-acetylated Tat.
Interacts with the host chemokine receptors CCR2, CCR3 and CXCR4.
Interacts with host DPP4/CD26; this interaction may trigger an anti-proliferative effect.
Interacts with host LDLR.
Interacts with the host extracellular matrix metalloproteinase MMP1.
Interacts with host PRMT6; this interaction mediates Tat's methylation.
Interacts with, and is ubiquitinated by MDM2/Hdm2.
Interacts with host PSMC3 and HTATIP2.
Interacts with STAB1; this interaction may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T cells by binding to the same domain than HDAC1.
Interacts (when acetylated) with human CDK13, thereby increasing HIV-1 mRNA splicing and promoting the production of the doubly spliced HIV-1 protein Nef.
Interacts with host TBP; this interaction modulates the activity of transcriptional pre-initiation complex.
Interacts with host RELA.
UniRule annotationGO - Molecular functioni
- actinin binding Source: UniProtKB-UniRule
- cyclin binding Source: UniProtKB-UniRule
- protein domain specific binding Source: UniProtKB-UniRule
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 48 | TransactivationUniRule annotationAdd BLAST | 48 | |
Regioni | 1 – 24 | Interaction with human CREBBPUniRule annotationAdd BLAST | 24 | |
Regioni | 22 – 37 | Cysteine-richUniRule annotationAdd BLAST | 16 | |
Regioni | 38 – 48 | CoreUniRule annotationAdd BLAST | 11 | |
Regioni | 47 – 101 | DisorderedSequence analysisAdd BLAST | 55 | |
Regioni | 49 – 86 | Interaction with the host capping enzyme RNGTTUniRule annotationAdd BLAST | 38 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 49 – 57 | Nuclear localization signal, RNA-binding (TAR), and protein transductionUniRule annotation | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 61 – 81 | PolarSequence analysisAdd BLAST | 21 | |
Compositional biasi | 82 – 101 | PolyampholyteSequence analysisAdd BLAST | 20 |
Domaini
Sequence similaritiesi
Family and domain databases
Gene3Di | 4.10.20.10, 1 hit |
HAMAPi | MF_04079, HIV_TAT, 1 hit |
InterProi | View protein in InterPro IPR001831, IV_Tat IPR036963, Tat_dom_sf |
Pfami | View protein in Pfam PF00539, Tat, 1 hit |
PRINTSi | PR00055, HIVTATDOMAIN |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MEPVDPNLEP WNHPGSQPKT ACNTCYCKKC SYHCLVCFQT KGLGISYGRK
60 70 80 90 100
KRRQRRSAPP SSEDHQNPIS KQPLPRTQGD PTGSEESKKK VESKTETDPF
D
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AH011084 Genomic DNA Translation: AAL06113.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AH011084 Genomic DNA Translation: AAL06113.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family and domain databases
Gene3Di | 4.10.20.10, 1 hit |
HAMAPi | MF_04079, HIV_TAT, 1 hit |
InterProi | View protein in InterPro IPR001831, IV_Tat IPR036963, Tat_dom_sf |
Pfami | View protein in Pfam PF00539, Tat, 1 hit |
PRINTSi | PR00055, HIVTATDOMAIN |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | Q900K4_9HIV1 | |
Accessioni | Q900K4Primary (citable) accession number: Q900K4 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | December 1, 2001 |
Last sequence update: | December 1, 2001 | |
Last modified: | October 7, 2020 | |
This is version 75 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |