UniProtKB - Q8ZZM5 (THI4_PYRAE)
Protein
Thiamine thiazole synthase
Gene
thi4
Organism
Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
Status
Functioni
Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation
Catalytic activityi
- glycine + hydrogen sulfide + NAD+ = ADP-5-ethyl-4-methylthiazole-2-carboxylate + H+ + 3 H2O + nicotinamideUniRule annotationEC:2.4.2.59UniRule annotation
Cofactori
Fe2+UniRule annotation
: thiamine diphosphate biosynthesis Pathwayi
This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 33 | NAD; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 60 | NAD; via amide nitrogen and carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 124 | NAD; via amide nitrogen and carbonyl oxygenUniRule annotation | 1 | |
Metal bindingi | 154 | Iron; shared with adjacent protomerUniRule annotation | 1 | |
Metal bindingi | 169 | IronUniRule annotation | 1 | |
Binding sitei | 219 | NAD; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 229 | GlycineUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 52 – 53 | NADUniRule annotation | 2 | |
Nucleotide bindingi | 152 – 154 | NAD; shared with adjacent protomerUniRule annotation | 3 |
GO - Molecular functioni
- iron ion binding Source: UniProtKB-UniRule
- transferase activity, transferring pentosyl groups Source: UniProtKB-UniRule
GO - Biological processi
- thiamine biosynthetic process Source: UniProtKB-UniRule
- thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
- thiazole biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Transferase |
Biological process | Thiamine biosynthesis |
Ligand | Iron, Metal-binding, NAD |
Enzyme and pathway databases
BioCyci | PAER178306:GCEO-125-MONOMER |
UniPathwayi | UPA00060 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:thi4UniRule annotation Ordered Locus Names:PAE0175 |
Organismi | Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827) |
Taxonomic identifieri | 178306 [NCBI] |
Taxonomic lineagei | Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000115613 | 1 – 261 | Thiamine thiazole synthaseAdd BLAST | 261 |
Interactioni
Subunit structurei
Homooctamer; tetramer of dimers.
UniRule annotationProtein-protein interaction databases
STRINGi | 178306.PAE0175 |
Family & Domainsi
Sequence similaritiesi
Belongs to the THI4 family.UniRule annotation
Phylogenomic databases
eggNOGi | arCOG00574 Archaea COG1635 LUCA |
HOGENOMi | HOG000106048 |
InParanoidi | Q8ZZM5 |
KOi | K22699 |
OMAi | MWGGGMM |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
HAMAPi | MF_00304 Thi4, 1 hit |
InterProi | View protein in InterPro IPR036188 FAD/NAD-bd_sf IPR002922 Thi4_fam IPR022828 Thi4_prok |
SUPFAMi | SSF51905 SSF51905, 1 hit |
TIGRFAMsi | TIGR00292 TIGR00292, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8ZZM5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MELKIGRAII SHALKDLDEY SDVDVAIVGA GPAGLTAARY LAEKGLKVVV
60 70 80 90 100
YERRFSFGGG IGPGGNMLPK IVVQEEAVPI LRDFKVRYKP AEDGLYTVDP
110 120 130 140 150
AELIAKLAAG AVDAGAKIIL GVHVDDVIFR GDPPRVTGLL WIWTPIQMSG
160 170 180 190 200
MHVDPLYTQA KAVIDATGHD AEVVSVAARK VPELGIQVVG EKSAWSEVSE
210 220 230 240 250
KLVVEHTGRV APGLYVAGIA VCAVYGLPRM GPIFGGMLMS GKKVAEVVYK
260
DLMAEAHAVR A
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE009441 Genomic DNA Translation: AAL62614.1 |
Genome annotation databases
EnsemblBacteriai | AAL62614; AAL62614; PAE0175 |
KEGGi | pai:PAE0175 |
PATRICi | fig|178306.9.peg.126 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE009441 Genomic DNA Translation: AAL62614.1 |
3D structure databases
SMRi | Q8ZZM5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 178306.PAE0175 |
Genome annotation databases
EnsemblBacteriai | AAL62614; AAL62614; PAE0175 |
KEGGi | pai:PAE0175 |
PATRICi | fig|178306.9.peg.126 |
Phylogenomic databases
eggNOGi | arCOG00574 Archaea COG1635 LUCA |
HOGENOMi | HOG000106048 |
InParanoidi | Q8ZZM5 |
KOi | K22699 |
OMAi | MWGGGMM |
Enzyme and pathway databases
UniPathwayi | UPA00060 |
BioCyci | PAER178306:GCEO-125-MONOMER |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
HAMAPi | MF_00304 Thi4, 1 hit |
InterProi | View protein in InterPro IPR036188 FAD/NAD-bd_sf IPR002922 Thi4_fam IPR022828 Thi4_prok |
SUPFAMi | SSF51905 SSF51905, 1 hit |
TIGRFAMsi | TIGR00292 TIGR00292, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | THI4_PYRAE | |
Accessioni | Q8ZZM5Primary (citable) accession number: Q8ZZM5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 24, 2009 |
Last sequence update: | March 1, 2002 | |
Last modified: | July 3, 2019 | |
This is version 98 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - PATHWAY comments
Index of metabolic and biosynthesis pathways