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UniProtKB - Q8ZL60 (Q8ZL60_SALTY)
Protein
tRNA (cytidine(34)-2'-O)-methyltransferase
Gene
yibK
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Functioni
Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide.
UniRule annotationCatalytic activityi
- 5-carboxymethylaminomethyluridine34 in tRNALeu + S-adenosyl-L-methionine = 5-carboxymethylaminomethyl-2'-O-methyluridine34 in tRNALeu + H+ + S-adenosyl-L-homocysteineUniRule annotationEC:2.1.1.207UniRule annotation
- cytidine34 in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine34 in tRNA + H+ + S-adenosyl-L-homocysteineUniRule annotationEC:2.1.1.207UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 78 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 100 | S-adenosyl-L-methionine; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 122 | S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 130 | S-adenosyl-L-methionineUniRule annotation | 1 |
GO - Molecular functioni
- RNA binding Source: InterPro
- S-adenosylmethionine-dependent methyltransferase activity Source: UniProtKB-UniRule
- tRNA methyltransferase activity Source: UniProtKB-UniRule
GO - Biological processi
- wobble position cytosine ribose methylation Source: GO_Central
- wobble position uridine ribose methylation Source: GO_Central
Keywordsi
Molecular function | MethyltransferaseUniRule annotationARBA annotation, Transferase |
Biological process | tRNA processingUniRule annotation |
Ligand | S-adenosyl-L-methionineUniRule annotation |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA (cytidine(34)-2'-O)-methyltransferaseUniRule annotation (EC:2.1.1.207UniRule annotation)Alternative name(s): tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmLUniRule annotation |
Gene namesi | Name:yibKImported Synonyms:trmLUniRule annotation Ordered Locus Names:STM3695Imported |
Organismi | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)Imported |
Taxonomic identifieri | 99287 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Salmonella › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmUniRule annotationInteractioni
Subunit structurei
Homodimer.
UniRule annotationProtein-protein interaction databases
STRINGi | 99287.STM3695 |
Structurei
3D structure databases
AlphaFoldDBi | Q8ZL60 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 142 | SpoU_methylaseInterPro annotationAdd BLAST | 141 |
Sequence similaritiesi
Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily.UniRule annotation
Phylogenomic databases
HOGENOMi | CLU_110125_1_0_6 |
OMAi | AGLDYWH |
PhylomeDBi | Q8ZL60 |
Family and domain databases
CDDi | cd18094, SpoU-like_TrmL, 1 hit |
Gene3Di | 3.40.1280.10, 1 hit |
HAMAPi | MF_01885, tRNA_methyltr_TrmL, 1 hit |
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR001537, SpoU_MeTrfase IPR016914, tRNA_cyt/urid_MeTfrase IPR029026, tRNA_m1G_MTases_N |
PANTHERi | PTHR42971, PTHR42971, 1 hit |
Pfami | View protein in Pfam PF00588, SpoU_methylase, 1 hit |
PIRSFi | PIRSF029256, SpoU_TrmH_prd, 1 hit |
SUPFAMi | SSF75217, SSF75217, 1 hit |
TIGRFAMsi | TIGR00185, tRNA_yibK_trmL, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8ZL60-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLNIVLFEPE IPPNTGNIIR LCANTGFRLH IIEPMGFTWD DKRLRRAGLD
60 70 80 90 100
YHEFAAVQRH HDYAAFVAAE NPQRLFALTT KGTPAHSAVS YQDGDYLMFG
110 120 130 140 150
PETRGLPASI LDALPKEQKI RIPMMPDSRS MNLSNAVSVV VYEAWRQLGY
PGAVLRS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE006468 Genomic DNA Translation: AAL22554.1 |
RefSeqi | NP_462595.1, NC_003197.2 WP_000932335.1, NC_003197.2 |
Genome annotation databases
EnsemblBacteriai | AAL22554; AAL22554; STM3695 |
GeneIDi | 1255219 |
KEGGi | stm:STM3695 |
PATRICi | fig|99287.12.peg.3908 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE006468 Genomic DNA Translation: AAL22554.1 |
RefSeqi | NP_462595.1, NC_003197.2 WP_000932335.1, NC_003197.2 |
3D structure databases
AlphaFoldDBi | Q8ZL60 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 99287.STM3695 |
Proteomic databases
PaxDbi | Q8ZL60 |
Genome annotation databases
EnsemblBacteriai | AAL22554; AAL22554; STM3695 |
GeneIDi | 1255219 |
KEGGi | stm:STM3695 |
PATRICi | fig|99287.12.peg.3908 |
Phylogenomic databases
HOGENOMi | CLU_110125_1_0_6 |
OMAi | AGLDYWH |
PhylomeDBi | Q8ZL60 |
Family and domain databases
CDDi | cd18094, SpoU-like_TrmL, 1 hit |
Gene3Di | 3.40.1280.10, 1 hit |
HAMAPi | MF_01885, tRNA_methyltr_TrmL, 1 hit |
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR001537, SpoU_MeTrfase IPR016914, tRNA_cyt/urid_MeTfrase IPR029026, tRNA_m1G_MTases_N |
PANTHERi | PTHR42971, PTHR42971, 1 hit |
Pfami | View protein in Pfam PF00588, SpoU_methylase, 1 hit |
PIRSFi | PIRSF029256, SpoU_TrmH_prd, 1 hit |
SUPFAMi | SSF75217, SSF75217, 1 hit |
TIGRFAMsi | TIGR00185, tRNA_yibK_trmL, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | Q8ZL60_SALTY | |
Accessioni | Q8ZL60Primary (citable) accession number: Q8ZL60 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | March 1, 2002 |
Last sequence update: | March 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 101 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |