yibK

Functionⁱ

Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide.UniRule annotation

Catalytic activityⁱ

S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu).UniRule annotation

S-adenosyl-L-methionine + cytidine(34) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(34) in tRNA.UniRule annotation

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	78	S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation	1
Binding siteⁱ	100	S-adenosyl-L-methionine; via amide nitrogenUniRule annotation	1
Binding siteⁱ	122	S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation	1
Binding siteⁱ	130	S-adenosyl-L-methionineUniRule annotation	1

GO - Molecular functionⁱ

RNA binding Source: InterPro
S-adenosylmethionine-dependent methyltransferase activity Source: UniProtKB-UniRule
tRNA methyltransferase activity Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	MethyltransferaseUniRule annotationSAAS annotationImported, Transferase
Biological process	tRNA processingUniRule annotation
Ligand	S-adenosyl-L-methionineUniRule annotation

Enzyme and pathway databases

BioCycⁱ	SENT99287:G1FZD-3732-MONOMER

BioCycⁱ

SENT99287:G1FZD-3732-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: tRNA (cytidine(34)-2'-O)-methyltransferaseUniRule annotation (EC:2.1.1.207UniRule annotation) Alternative name(s): tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmLUniRule annotation
Gene namesⁱ	Name:yibKImported Synonyms:trmLUniRule annotation Ordered Locus Names:STM3695Imported
Organismⁱ	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)Imported
Taxonomic identifierⁱ	99287 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium
Proteomesⁱ	UP000001014 Componentⁱ: Chromosome

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Automatic assertion according to rulesⁱ
- HAMAP-Rule:MF_01885

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

CytoplasmUniRule annotation

PTM / Processingⁱ

Proteomic databases

PaxDbⁱ	Q8ZL60
PRIDEⁱ	Q8ZL60

Interactionⁱ

Subunit structureⁱ

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGⁱ	99287.STM3695

STRINGⁱ

99287.STM3695

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8ZL60
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	2 – 142	SpoU_methylaseInterPro annotationAdd BLAST		141

Sequence similaritiesⁱ

Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4108UIQ Bacteria COG0219 LUCA
HOGENOMⁱ	HOG000272756
KEGG Orthology (KO) More...KOⁱ	K03216
OMAⁱ	AGLDYWH
PhylomeDBⁱ	Q8ZL60

Family and domain databases

Gene3Dⁱ	3.40.1280.10, 1 hit
HAMAPⁱ	MF_01885 tRNA_methyltr_TrmL, 1 hit
InterProⁱ	View protein in InterPro IPR029028 Alpha/beta_knot_MTases IPR001537 SpoU_MeTrfase IPR016914 tRNA_cyt/urid_MeTfrase IPR029026 tRNA_m1G_MTases_N
PANTHERⁱ	PTHR42971 PTHR42971, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00588 SpoU_methylase, 1 hit
PIRSFⁱ	PIRSF029256 SpoU_TrmH_prd, 1 hit
SUPFAMⁱ	SSF75217 SSF75217, 1 hit
TIGRFAMsⁱ	TIGR00185 tRNA_yibK_trmL, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q8ZL60-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MLNIVLFEPE IPPNTGNIIR LCANTGFRLH IIEPMGFTWD DKRLRRAGLD 
        60         70         80         90        100
YHEFAAVQRH HDYAAFVAAE NPQRLFALTT KGTPAHSAVS YQDGDYLMFG 
       110        120        130        140        150
PETRGLPASI LDALPKEQKI RIPMMPDSRS MNLSNAVSVV VYEAWRQLGY 

PGAVLRS

Length:157

Mass (Da):17,641

Last modified:March 1, 2002 - v1

Checksum:ⁱAD0E1D36BE19541F

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE006468 Genomic DNA Translation: AAL22554.1
NCBI Reference Sequences More...RefSeqⁱ	NP_462595.1, NC_003197.2 WP_000932335.1, NC_003197.2

Genome annotation databases

EnsemblBacteriaⁱ	AAL22554; AAL22554; STM3695
GeneIDⁱ	1255219
KEGGⁱ	stm:STM3695
PATRICⁱ	fig\|99287.12.peg.3908

Protein	Similar proteins		Species	Score	Length	Source
Q8ZL60	tRNA (Uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL		SALDE		157	UniRef100_A0A2W4E292
tRNA (Uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL		Salmonella enterica subsp. enterica serovar Newport str. A-211-RVH		157
RNA-methyltransferase		Salmonella enterica subsp. enterica serovar Bredeney		157
RNA-methyltransferase		Salmonella enterica subsp. enterica serovar Stanley		157
RNA-methyltransferase		Salmonella enterica subsp. enterica serovar Poona		157
+42

Protein	Similar proteins		Species	Score	Length	Source
Q8ZL60	tRNA (cytidine(34)-2'-O)-methyltransferase		ECOL6		157	UniRef90_P0AGJ8
tRNA (cytidine(34)-2'-O)-methyltransferase	)	ECOLI		157
tRNA (cytidine(34)-2'-O)-methyltransferase		ECOLX		157
tRNA (cytidine(34)-2'-O)-methyltransferase		SHISO		157
tRNA (cytidine(34)-2'-O)-methyltransferase		Escherichia coli 1-110-08_S3_C1		157
+771

Protein	Similar proteins		Species	Score	Length	Source
Q8ZL60	tRNA (cytidine(34)-2'-O)-methyltransferase		ECOL6		157	UniRef50_P0AGJ8
tRNA (cytidine(34)-2'-O)-methyltransferase	)	ECOLI		157
tRNA (cytidine(34)-2'-O)-methyltransferase		ECOLX		157
tRNA (cytidine(34)-2'-O)-methyltransferase		SHISO		157
tRNA (cytidine(34)-2'-O)-methyltransferase		Escherichia coli 1-110-08_S3_C1		157
+846

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE006468 Genomic DNA Translation: AAL22554.1
RefSeqⁱ	NP_462595.1, NC_003197.2 WP_000932335.1, NC_003197.2

3D structure databases

ProteinModelPortalⁱ	Q8ZL60
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	99287.STM3695

STRINGⁱ

99287.STM3695

Proteomic databases

PaxDbⁱ	Q8ZL60
PRIDEⁱ	Q8ZL60

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAL22554; AAL22554; STM3695
GeneIDⁱ	1255219
KEGGⁱ	stm:STM3695
PATRICⁱ	fig\|99287.12.peg.3908

Phylogenomic databases

eggNOGⁱ	ENOG4108UIQ Bacteria COG0219 LUCA
HOGENOMⁱ	HOG000272756
KOⁱ	K03216
OMAⁱ	AGLDYWH
PhylomeDBⁱ	Q8ZL60

Enzyme and pathway databases

BioCycⁱ	SENT99287:G1FZD-3732-MONOMER

BioCycⁱ

SENT99287:G1FZD-3732-MONOMER

Family and domain databases

Gene3Dⁱ	3.40.1280.10, 1 hit
HAMAPⁱ	MF_01885 tRNA_methyltr_TrmL, 1 hit
InterProⁱ	View protein in InterPro IPR029028 Alpha/beta_knot_MTases IPR001537 SpoU_MeTrfase IPR016914 tRNA_cyt/urid_MeTfrase IPR029026 tRNA_m1G_MTases_N
PANTHERⁱ	PTHR42971 PTHR42971, 1 hit
Pfamⁱ	View protein in Pfam PF00588 SpoU_methylase, 1 hit
PIRSFⁱ	PIRSF029256 SpoU_TrmH_prd, 1 hit
SUPFAMⁱ	SSF75217 SSF75217, 1 hit
TIGRFAMsⁱ	TIGR00185 tRNA_yibK_trmL, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	Q8ZL60_SALTY
Accessionⁱ	Q8ZL60Primary (citable) accession number: Q8ZL60
Entry historyⁱ	Integrated into UniProtKB/TrEMBL:	March 1, 2002
	Last sequence update:	March 1, 2002
	Last modified:	February 28, 2018
	This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Unreviewed (UniProtKB/TrEMBL)

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteomeImported

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q8ZL60 (Q8ZL60_SALTY)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

tRNA (cytidine(34)-2'-O)-methyltransferase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ