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Entry version 111 (26 Feb 2020)
Sequence version 1 (01 Mar 2002)
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Protein

tRNA-dihydrouridine(20/20a) synthase

Gene

dusA

Organism
Yersinia pestis
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs.UniRule annotation

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

FMNUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei78FMNUniRule annotation1
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei105Interacts with tRNAUniRule annotation1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei108Proton donorUniRule annotation1
Binding sitei147FMNUniRule annotation1
Binding sitei180FMNUniRule annotation1
Sitei192Interacts with tRNA; defines subfamily-specific binding signatureUniRule annotation1
Sitei195Interacts with tRNAUniRule annotation1
Sitei308Interacts with tRNA; defines subfamily-specific binding signatureUniRule annotation1
Sitei311Interacts with tRNA; defines subfamily-specific binding signatureUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi26 – 28FMNUniRule annotation3
Nucleotide bindingi220 – 222FMNUniRule annotation3
Nucleotide bindingi242 – 243FMNUniRule annotation2

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase, RNA-binding, tRNA-binding
Biological processtRNA processing
LigandFlavoprotein, FMN, NADP

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
tRNA-dihydrouridine(20/20a) synthaseUniRule annotation (EC:1.3.1.-UniRule annotation, EC:1.3.1.91UniRule annotation)
Alternative name(s):
U20-specific dihydrouridine synthaseUniRule annotation
Short name:
U20-specific DusUniRule annotation
tRNA-dihydrouridine synthase AUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:dusAUniRule annotation
Ordered Locus Names:YPO0316, y0574, YP_0472
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiYersinia pestis
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri632 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000815 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001620831 – 345tRNA-dihydrouridine(20/20a) synthaseAdd BLAST345

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q8ZJ14

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
214092.YPO0316

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q8ZJ14

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the Dus family. DusA subfamily.UniRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105CEH Bacteria
COG0042 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_013299_2_1_6

KEGG Orthology (KO)

More...
KOi
K05539

Family and domain databases

Conserved Domains Database

More...
CDDi
cd02801 DUS_like_FMN, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.20.20.70, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_02041 DusA_subfam, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR013785 Aldolase_TIM
IPR035587 DUS-like_FMN-bd
IPR004653 DusA
IPR001269 tRNA_hU_synthase
IPR018517 tRNA_hU_synthase_CS

The PANTHER Classification System

More...
PANTHERi
PTHR42907 PTHR42907, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01207 Dus, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF006621 Dus, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00742 yjbN, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS01136 UPF0034, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q8ZJ14-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHEAQTFSST PATKPQYPLQ RFSVAPMLDW TDRHCRYFHR LLTKQALLYT
60 70 80 90 100
EMVTTGAIIH GKADYLAYSE QDHPVALQLG GSDPQALAHC AKLAEQRGYN
110 120 130 140 150
EINLNVGCPS DRVQNGRFGA CLMGEADLVA DCIKAMRDAV AIPVTVKTRI
160 170 180 190 200
GIDQLDSYEF LCEFVQTVAE RGECEIFTIH ARKAWLSGLS PKENREVPPL
210 220 230 240 250
DYERVYQLKR DFPALTIAIN GGVKTLAEAK EHLKHLDGVM MGREAYQNPG
260 270 280 290 300
ILTQVDRELF DPNAPVVDSV KAIEALYPYI EQELSQGAYL GHITRHILGI
310 320 330 340
FQGIPGARQW RRHLSENAHK PGAGVSVVEE ALALVSPSYY ESVGG
Length:345
Mass (Da):38,333
Last modified:March 1, 2002 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iA8CD020A46D6213C
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AL590842 Genomic DNA Translation: CAL19001.1
AE009952 Genomic DNA Translation: AAM84162.1
AE017042 Genomic DNA Translation: AAS60742.1

Protein sequence database of the Protein Information Resource

More...
PIRi
AG0039

NCBI Reference Sequences

More...
RefSeqi
WP_002209092.1, NZ_VWSA01000088.1
YP_002345397.1, NC_003143.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAM84162; AAM84162; y0574
AAS60742; AAS60742; YP_0472

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
1173161

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ype:YPO0316
ypk:y0574
ypm:YP_0472

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|214092.21.peg.552

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA Translation: CAL19001.1
AE009952 Genomic DNA Translation: AAM84162.1
AE017042 Genomic DNA Translation: AAS60742.1
PIRiAG0039
RefSeqiWP_002209092.1, NZ_VWSA01000088.1
YP_002345397.1, NC_003143.1

3D structure databases

SMRiQ8ZJ14
ModBaseiSearch...

Protein-protein interaction databases

STRINGi214092.YPO0316

Proteomic databases

PaxDbiQ8ZJ14

Protocols and materials databases

The DNASU plasmid repository

More...
DNASUi
1145521

Genome annotation databases

EnsemblBacteriaiAAM84162; AAM84162; y0574
AAS60742; AAS60742; YP_0472
GeneIDi1173161
KEGGiype:YPO0316
ypk:y0574
ypm:YP_0472
PATRICifig|214092.21.peg.552

Phylogenomic databases

eggNOGiENOG4105CEH Bacteria
COG0042 LUCA
HOGENOMiCLU_013299_2_1_6
KOiK05539

Family and domain databases

CDDicd02801 DUS_like_FMN, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_02041 DusA_subfam, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR035587 DUS-like_FMN-bd
IPR004653 DusA
IPR001269 tRNA_hU_synthase
IPR018517 tRNA_hU_synthase_CS
PANTHERiPTHR42907 PTHR42907, 1 hit
PfamiView protein in Pfam
PF01207 Dus, 1 hit
PIRSFiPIRSF006621 Dus, 1 hit
TIGRFAMsiTIGR00742 yjbN, 1 hit
PROSITEiView protein in PROSITE
PS01136 UPF0034, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiDUSA_YERPE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8ZJ14
Secondary accession number(s): Q0WJZ1
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: March 1, 2002
Last modified: February 26, 2020
This is version 111 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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