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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Yersinia pestis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Activity regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
LigandMagnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:YPO1042, y3139, YP_2809
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001927801 – 893Bifunctional uridylyltransferase/uridylyl-removing enzymeAdd BLAST893

Proteomic databases

PaxDbiQ8ZH68

Interactioni

Protein-protein interaction databases

STRINGi187410.y3139

Structurei

3D structure databases

ProteinModelPortaliQ8ZH68
SMRiQ8ZH68
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini470 – 592HDPROSITE-ProRule annotationAdd BLAST123
Domaini711 – 793ACT 1UniRule annotationAdd BLAST83
Domaini819 – 893ACT 2UniRule annotationAdd BLAST75

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 351UridylyltransferaseAdd BLAST351
Regioni352 – 710Uridylyl-removingAdd BLAST359

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105E1P Bacteria
COG2844 LUCA
HOGENOMiHOG000261778
KOiK00990
OMAiARICGYF

Family and domain databases

CDDicd00077 HDc, 1 hit
HAMAPiMF_00277 PII_uridylyl_transf, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR003607 HD/PDEase_dom
IPR006674 HD_domain
IPR013546 PII_UdlTrfase/GS_AdlTrfase
IPR002934 Polymerase_NTP_transf_dom
IPR010043 UTase/UR
PANTHERiPTHR13734:SF45 PTHR13734:SF45, 1 hit
PfamiView protein in Pfam
PF01842 ACT, 1 hit
PF08335 GlnD_UR_UTase, 1 hit
PF01966 HD, 1 hit
PF01909 NTP_transf_2, 1 hit
PIRSFiPIRSF006288 PII_uridyltransf, 1 hit
SMARTiView protein in SMART
SM00471 HDc, 1 hit
TIGRFAMsiTIGR01693 UTase_glnD, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 2 hits
PS51831 HD, 1 hit

Sequencei

Sequence statusi: Complete.

Q8ZH68-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSDNHTEHSL SLTLTPTISE QPALPSTYLD SDIHCPILKQ RLDAFQRWQA
60 70 80 90 100
EAFNSGTSAE VLIAARSDYI DHLLQRLWTF YGFDKVPETA LVAVGGYGRG
110 120 130 140 150
ELHPLSDIDV LVLSKQRLND EQAQRVGQLI TLLWDLKLEV GHSVRTLEEC
160 170 180 190 200
LLEGLADLTI ATNMIESRLI CGDVALFLQM QKHIFSDSFW PSPQFFHAKV
210 220 230 240 250
VEQQERHKRY HGTSYNLEPD IKSSPGGLRD IHTLLWVARR HFGATSLSEM
260 270 280 290 300
VDFGFLTNAE RNELNESQSF LWRIRFALHL VLTRYDNRLL FDRQLSVAQL
310 320 330 340 350
LRYEGEGNEP VEHMMKDFYR MTRRVSELNN MLLQLFDEAI LALDANEKPR
360 370 380 390 400
PLDEEFQLRG DLIDLRDENL FVRQPEAIMR MFYLMVRNQD IKGIYSTTVR
410 420 430 440 450
RLRHARRHLK APLCHIPEAR KLFMAILRHP GAVSRALLPM HRHSVLWAYM
460 470 480 490 500
PQWGSIVGQM QFDLFHAYTV DEHTIRVLLK IESFADEDTR PRHPLCVELY
510 520 530 540 550
PRLPQPELLL LAALFHDIAK GRGGDHSILG AHDAVEFAEQ HGLNSRESQL
560 570 580 590 600
VAWLVRCHLL MSVTAQRRDI QDPAVIQQFS AEVQSETRLR YLVSLTVADI
610 620 630 640 650
CATNENLWNS WKQSLLRELY FATEKQLRRG MQNSPDLRER VRHHRLQALA
660 670 680 690 700
LLRMDNIDEE ALHRIWSRCR ADYFLRHSPN QLAWHARHLL EHDSTKPLVL
710 720 730 740 750
VSRQATRGGT EIFIWSPDRP SLFAAVVGEL DRRNLSVHDA QIFTNRDGMA
760 770 780 790 800
MDTFIVLEPD GSPLAQDRHP IISHALQQAI NRSDYQHPPR VRRLSPKLRH
810 820 830 840 850
FSVPTEANFL PTHNERRTYL ELIALDQPGL LARVGKIFAD LGLSLHSARI
860 870 880 890
TTIGERVEDL FVLADKDRRA LSLETRRELA QRLADTLNPN DKL
Length:893
Mass (Da):103,158
Last modified:June 6, 2002 - v2
Checksum:iAE09A7808DB3D611
GO

Sequence cautioni

The sequence AAM86689 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAS62993 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAL19707 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA Translation: CAL19707.1 Different initiation.
AE009952 Genomic DNA Translation: AAM86689.1 Different initiation.
AE017042 Genomic DNA Translation: AAS62993.1 Different initiation.
PIRiAI0127
RefSeqiWP_002212129.1, NZ_PVMU01000045.1
YP_002346085.1, NC_003143.1

Genome annotation databases

EnsemblBacteriaiAAM86689; AAM86689; y3139
AAS62993; AAS62993; YP_2809
GeneIDi1173882
KEGGiype:YPO1042
ypk:y3139
ypm:YP_2809
PATRICifig|214092.21.peg.1330

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA Translation: CAL19707.1 Different initiation.
AE009952 Genomic DNA Translation: AAM86689.1 Different initiation.
AE017042 Genomic DNA Translation: AAS62993.1 Different initiation.
PIRiAI0127
RefSeqiWP_002212129.1, NZ_PVMU01000045.1
YP_002346085.1, NC_003143.1

3D structure databases

ProteinModelPortaliQ8ZH68
SMRiQ8ZH68
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187410.y3139

Proteomic databases

PaxDbiQ8ZH68

Protocols and materials databases

DNASUi1148086
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM86689; AAM86689; y3139
AAS62993; AAS62993; YP_2809
GeneIDi1173882
KEGGiype:YPO1042
ypk:y3139
ypm:YP_2809
PATRICifig|214092.21.peg.1330

Phylogenomic databases

eggNOGiENOG4105E1P Bacteria
COG2844 LUCA
HOGENOMiHOG000261778
KOiK00990
OMAiARICGYF

Family and domain databases

CDDicd00077 HDc, 1 hit
HAMAPiMF_00277 PII_uridylyl_transf, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR003607 HD/PDEase_dom
IPR006674 HD_domain
IPR013546 PII_UdlTrfase/GS_AdlTrfase
IPR002934 Polymerase_NTP_transf_dom
IPR010043 UTase/UR
PANTHERiPTHR13734:SF45 PTHR13734:SF45, 1 hit
PfamiView protein in Pfam
PF01842 ACT, 1 hit
PF08335 GlnD_UR_UTase, 1 hit
PF01966 HD, 1 hit
PF01909 NTP_transf_2, 1 hit
PIRSFiPIRSF006288 PII_uridyltransf, 1 hit
SMARTiView protein in SMART
SM00471 HDc, 1 hit
TIGRFAMsiTIGR01693 UTase_glnD, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 2 hits
PS51831 HD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGLND_YERPE
AccessioniPrimary (citable) accession number: Q8ZH68
Secondary accession number(s): Q0WI03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: June 6, 2002
Last modified: October 10, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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