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Protein

Carbamoyl-phosphate synthase small chain

Gene

carA

Organism
Salmonella typhi
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA)
  2. Aspartate carbamoyltransferase catalytic subunit (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei269NucleophileUniRule annotation1
Active sitei353UniRule annotation1
Active sitei355UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis, Pyrimidine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00068;UER00171

UPA00070;UER00115

Protein family/group databases

MEROPSiC26.954

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase small chainUniRule annotation (EC:6.3.5.5UniRule annotation)
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chainUniRule annotation
Gene namesi
Name:carAUniRule annotation
Ordered Locus Names:STY0076, t0067
OrganismiSalmonella typhi
Taxonomic identifieri90370 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000002670 Componenti: Chromosome
  • UP000000541 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001123121 – 382Carbamoyl-phosphate synthase small chainAdd BLAST382

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotation

Protein-protein interaction databases

STRINGi220341.STY0076

Structurei

3D structure databases

ProteinModelPortaliQ8Z9L8
SMRiQ8Z9L8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini193 – 380Glutamine amidotransferase type-1UniRule annotationAdd BLAST188

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 189CPSaseAdd BLAST189

Sequence similaritiesi

Belongs to the CarA family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105C1M Bacteria
COG0505 LUCA
HOGENOMiHOG000038087
KOiK01956

Family and domain databases

CDDicd01744 GATase1_CPSase, 1 hit
Gene3Di3.40.50.880, 1 hit
3.50.30.20, 1 hit
HAMAPiMF_01209 CPSase_S_chain, 1 hit
InterProiView protein in InterPro
IPR006274 CarbamoylP_synth_ssu
IPR002474 CarbamoylP_synth_ssu_N
IPR036480 CarbP_synth_ssu_N_sf
IPR029062 Class_I_gatase-like
IPR035686 CPSase_GATase1
IPR017926 GATASE
PfamiView protein in Pfam
PF00988 CPSase_sm_chain, 1 hit
PF00117 GATase, 1 hit
SMARTiView protein in SMART
SM01097 CPSase_sm_chain, 1 hit
SUPFAMiSSF52021 SSF52021, 1 hit
SSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR01368 CPSaseIIsmall, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q8Z9L8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIKSALLVLE DGTQFHGRAI GATGSAVGEV VFNTSMTGYQ EILTDPSYSR
60 70 80 90 100
QIVTLTYPHI GNVGTNEADE ESSQVHAQGL VIRDLPLIAS NFRNTEDLSS
110 120 130 140 150
YLKRHNIVAI ADIDTRKLTR LLREKGAQNG CIIAGDSPDA KLALEKAKAF
160 170 180 190 200
PGLNGMDLAK EVTTAETYRW TQGSWTLKDG LPEAKSEDDL PFHVVAYDFG
210 220 230 240 250
AKRNILRMLV DRGCRLTVVP AQTSAEEVLK MNPDGIFLSN GPSDPAPCDY
260 270 280 290 300
AIEAIKKFLQ TDIPVFGICL GHQLLALASG AKTVKMKFGH HGGNHPVKDM
310 320 330 340 350
DRNVVMITAQ NHGFAVDEDS LPANLRVTHK SLFDGTLQGI HRTDKPAFSF
360 370 380
QGHPEASPGP HDAAPLFDHF IELIKHYRSS AK
Length:382
Mass (Da):41,662
Last modified:March 1, 2002 - v1
Checksum:iAB5F7C87AFB72DC6
GO

Sequence cautioni

The sequence AAB66481 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti243S → G in AAB66481 (PubMed:12644504).Curated1
Sequence conflicti243S → G in AAO67800 (PubMed:12644504).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012246 Genomic DNA Translation: AAB66481.1 Frameshift.
AL513382 Genomic DNA Translation: CAD01220.1
AE014613 Genomic DNA Translation: AAO67800.1
RefSeqiNP_454676.1, NC_003198.1
WP_000597281.1, NZ_CIUT01000001.1

Genome annotation databases

EnsemblBacteriaiAAO67800; AAO67800; t0067
CAD01220; CAD01220; CAD01220
GeneIDi1246578
KEGGistt:t0067
sty:STY0076
PATRICifig|220341.7.peg.75

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012246 Genomic DNA Translation: AAB66481.1 Frameshift.
AL513382 Genomic DNA Translation: CAD01220.1
AE014613 Genomic DNA Translation: AAO67800.1
RefSeqiNP_454676.1, NC_003198.1
WP_000597281.1, NZ_CIUT01000001.1

3D structure databases

ProteinModelPortaliQ8Z9L8
SMRiQ8Z9L8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220341.STY0076

Protein family/group databases

MEROPSiC26.954

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO67800; AAO67800; t0067
CAD01220; CAD01220; CAD01220
GeneIDi1246578
KEGGistt:t0067
sty:STY0076
PATRICifig|220341.7.peg.75

Phylogenomic databases

eggNOGiENOG4105C1M Bacteria
COG0505 LUCA
HOGENOMiHOG000038087
KOiK01956

Enzyme and pathway databases

UniPathwayi
UPA00068;UER00171

UPA00070;UER00115

Family and domain databases

CDDicd01744 GATase1_CPSase, 1 hit
Gene3Di3.40.50.880, 1 hit
3.50.30.20, 1 hit
HAMAPiMF_01209 CPSase_S_chain, 1 hit
InterProiView protein in InterPro
IPR006274 CarbamoylP_synth_ssu
IPR002474 CarbamoylP_synth_ssu_N
IPR036480 CarbP_synth_ssu_N_sf
IPR029062 Class_I_gatase-like
IPR035686 CPSase_GATase1
IPR017926 GATASE
PfamiView protein in Pfam
PF00988 CPSase_sm_chain, 1 hit
PF00117 GATase, 1 hit
SMARTiView protein in SMART
SM01097 CPSase_sm_chain, 1 hit
SUPFAMiSSF52021 SSF52021, 1 hit
SSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR01368 CPSaseIIsmall, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCARA_SALTI
AccessioniPrimary (citable) accession number: Q8Z9L8
Secondary accession number(s): O30576
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: March 1, 2002
Last modified: November 7, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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