UniProtKB - Q8Z591 (PTFAH_SALTI)
Protein
Multiphosphoryl transfer protein
Gene
fruB
Organism
Salmonella typhi
Status
Functioni
The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II FruAB PTS system is involved in fructose transport.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 62 | Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation | 1 | |
Active sitei | 299 | Pros-phosphohistidine intermediate; for HPr activityPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- kinase activity Source: UniProtKB-KW
GO - Biological processi
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Phosphotransferase system, Sugar transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Multiphosphoryl transfer proteinBy similarityShort name: MTPBy similarity Alternative name(s): Diphosphoryl transfer proteinBy similarity Short name: DTPBy similarity Phosphotransferase FPr proteinBy similarity Pseudo-HPrBy similarity Including the following 2 domains: Phosphocarrier protein HPrBy similarity Short name: Protein HBy similarity PTS system fructose-specific EIIA componentBy similarity Alternative name(s): EIIA-FruBy similarity EIII-FruBy similarity Fructose-specific phosphotransferase enzyme IIA componentBy similarity |
Gene namesi | Name:fruB Ordered Locus Names:STY2442, t0650 |
Organismi | Salmonella typhi |
Taxonomic identifieri | 90370 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Salmonella › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186518 | 1 – 376 | Multiphosphoryl transfer proteinAdd BLAST | 376 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 62 | Phosphohistidine; by HPrCurated | 1 | |
Modified residuei | 299 | Phosphohistidine; by EICurated | 1 |
Keywords - PTMi
PhosphoproteinExpressioni
Inductioni
Induced by fructose and repressed by FruR.By similarity
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 142 | PTS EIIA type-2PROSITE-ProRule annotationAdd BLAST | 141 | |
Domaini | 285 – 375 | HPrPROSITE-ProRule annotationAdd BLAST | 91 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 156 – 284 | M domainBy similarityAdd BLAST | 129 |
Domaini
The PTS EIIA type-2 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-2 domain.PROSITE-ProRule annotation
In contrast to classical PTS systems, the fructose-specific PTS has no requirement for HPr; FruB combines a IIA domain with a HPr domain.By similarity
Phylogenomic databases
eggNOGi | COG1925, Bacteria COG4668, Bacteria |
HOGENOMi | CLU_046384_0_0_6 |
Family and domain databases
CDDi | cd00367, PTS-HPr_like, 1 hit cd00211, PTS_IIA_fru, 1 hit |
Gene3Di | 3.30.1340.10, 1 hit 3.40.930.10, 1 hit |
InterProi | View protein in InterPro IPR000032, HPr-like IPR035895, HPr-like_sf IPR016152, PTrfase/Anion_transptr IPR002178, PTS_EIIA_type-2_dom IPR001020, PTS_HPr_His_P_site IPR002114, PTS_HPr_Ser_P_site |
Pfami | View protein in Pfam PF00381, PTS-HPr, 1 hit PF00359, PTS_EIIA_2, 1 hit |
PRINTSi | PR00107, PHOSPHOCPHPR |
SUPFAMi | SSF55594, SSF55594, 1 hit SSF55804, SSF55804, 2 hits |
TIGRFAMsi | TIGR01003, PTS_HPr_family, 1 hit |
PROSITEi | View protein in PROSITE PS51094, PTS_EIIA_TYPE_2, 1 hit PS00372, PTS_EIIA_TYPE_2_HIS, 1 hit PS51350, PTS_HPR_DOM, 1 hit PS00369, PTS_HPR_HIS, 1 hit PS00589, PTS_HPR_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8Z591-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFQLSVQDIH PGEQVGNKEE AIRQIAAALA QAGNVAGGYV DGMLAREQQT
60 70 80 90 100
STFLGNGIAI PHGTTDTRDQ VLKTGVQVFQ FPQGVTWGEG QVAYVAIGIA
110 120 130 140 150
ASSDEHLGLL RQLTHVLSDD SVAEQLKSAT TAEELRALLM GEKQSEQLKL
160 170 180 190 200
DNETMTLDVI ASSLVTLQAL NAARLKEAGA VDAAFVAKTI NDSPMNLGQG
210 220 230 240 250
IWLNDSAEGN LRSAVAVSRA TQAFDVEGEK AALLVTVAMN DEQPIAVLKR
260 270 280 290 300
LGDLLLNNKA DRLLSADAAT LLALLTSDDA LTDDVLSAEF VVRNEHGLHA
310 320 330 340 350
RPGTMLVNTI KQFNSEITVT NLDGTGKPAN GRSLMKVVAL GVKKGHRLRF
360 370
TAQGEDAEQA LKAIGDAIAA GLGEGA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 15 | V → A in AAO68350 (PubMed:12644504).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL513382 Genomic DNA Translation: CAD02588.1 AE014613 Genomic DNA Translation: AAO68350.1 |
RefSeqi | NP_456764.1, NC_003198.1 WP_000487294.1, NZ_PZMG01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAO68350; AAO68350; t0650 |
KEGGi | stt:t0650 sty:STY2442 |
PATRICi | fig|220341.7.peg.2468 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL513382 Genomic DNA Translation: CAD02588.1 AE014613 Genomic DNA Translation: AAO68350.1 |
RefSeqi | NP_456764.1, NC_003198.1 WP_000487294.1, NZ_PZMG01000001.1 |
3D structure databases
SMRi | Q8Z591 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 220341.16503445 |
Genome annotation databases
EnsemblBacteriai | AAO68350; AAO68350; t0650 |
KEGGi | stt:t0650 sty:STY2442 |
PATRICi | fig|220341.7.peg.2468 |
Phylogenomic databases
eggNOGi | COG1925, Bacteria COG4668, Bacteria |
HOGENOMi | CLU_046384_0_0_6 |
Family and domain databases
CDDi | cd00367, PTS-HPr_like, 1 hit cd00211, PTS_IIA_fru, 1 hit |
Gene3Di | 3.30.1340.10, 1 hit 3.40.930.10, 1 hit |
InterProi | View protein in InterPro IPR000032, HPr-like IPR035895, HPr-like_sf IPR016152, PTrfase/Anion_transptr IPR002178, PTS_EIIA_type-2_dom IPR001020, PTS_HPr_His_P_site IPR002114, PTS_HPr_Ser_P_site |
Pfami | View protein in Pfam PF00381, PTS-HPr, 1 hit PF00359, PTS_EIIA_2, 1 hit |
PRINTSi | PR00107, PHOSPHOCPHPR |
SUPFAMi | SSF55594, SSF55594, 1 hit SSF55804, SSF55804, 2 hits |
TIGRFAMsi | TIGR01003, PTS_HPr_family, 1 hit |
PROSITEi | View protein in PROSITE PS51094, PTS_EIIA_TYPE_2, 1 hit PS00372, PTS_EIIA_TYPE_2_HIS, 1 hit PS51350, PTS_HPR_DOM, 1 hit PS00369, PTS_HPR_HIS, 1 hit PS00589, PTS_HPR_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PTFAH_SALTI | |
Accessioni | Q8Z591Primary (citable) accession number: Q8Z591 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 23, 2003 |
Last sequence update: | March 1, 2002 | |
Last modified: | April 7, 2021 | |
This is version 119 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |