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Protein

Proline--tRNA ligase

Gene

proS

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).UniRule annotation

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
Alternative name(s):
Prolyl-tRNA synthetaseUniRule annotation
Short name:
ProRSUniRule annotation
Gene namesi
Name:proSUniRule annotation
Ordered Locus Names:BMEI1140
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000419 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002488941 – 442Proline--tRNA ligaseAdd BLAST442

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi224914.BAWG_1069

Structurei

3D structure databases

ProteinModelPortaliQ8YGL8
SMRiQ8YGL8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C90 Bacteria
COG0442 LUCA
HOGENOMiHOG000076894
KOiK01881
OMAiIQPAELW

Family and domain databases

CDDicd00779 ProRS_core_prok, 1 hit
Gene3Di3.40.50.800, 1 hit
HAMAPiMF_01570 Pro_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR002316 Pro-tRNA-ligase_IIa
IPR004500 Pro-tRNA-synth_IIa_bac-type
IPR023716 Prolyl-tRNA_ligase_IIa_type2
IPR033730 ProRS_core_prok
PfamiView protein in Pfam
PF03129 HGTP_anticodon, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PRINTSiPR01046 TRNASYNTHPRO
TIGRFAMsiTIGR00409 proS_fam_II, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

Q8YGL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLSRYFLPI LKENPKEAEI VSHRLMLRSG MIRQQSAGIY SWLPIGLKVL
60 70 80 90 100
NKVCTIIREE QNRAGANEIL MPTIQSADLW RESGRYDAYG KEMLRIQDRQ
110 120 130 140 150
KREMLFGPTN EEMVTDIFRS YVRSYKDLPL NLYHIQWKFR DEVRPRFGVM
160 170 180 190 200
RSREFLMKDA YSFDLDYEGA KMAYYRMFVS YLRTFARVGL QAIPMRADTG
210 220 230 240 250
PIGGDLSHEF IILAETGESQ VYCDRAYLDL AVPGADTDFR NDAQLTDTVT
260 270 280 290 300
RWTTPYAATD EMHDEADWAK VKPESQVSAR GIEVGHIFHF GTKYSEPMGA
310 320 330 340 350
KVQGPDGKEH LVSMGSYGIG PSRLVAAAIE ASHDDAGIIW PKTIAPFGAG
360 370 380 390 400
IVNMKPGDEG CDGVSEKLYE ALTNAGVDPL LDDKDERPGA KFATIDLIGL
410 420 430 440
PTQVIVGPRG VAAGEVEVKD RKTGERQSLG IEAAINMLTA QA
Length:442
Mass (Da):49,477
Last modified:September 5, 2006 - v2
Checksum:i4E9AD5F6F4AD5AB8
GO

Sequence cautioni

The sequence AAL52321 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA Translation: AAL52321.1 Different initiation.
PIRiAF3394
RefSeqiWP_004683631.1, NZ_GG703778.1

Genome annotation databases

EnsemblBacteriaiAAL52321; AAL52321; BMEI1140
GeneIDi29593980
KEGGibme:BMEI1140
bmel:DK63_273
PATRICifig|224914.52.peg.282

Similar proteinsi

Entry informationi

Entry nameiSYP_BRUME
AccessioniPrimary (citable) accession number: Q8YGL8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: February 28, 2018
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

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