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Protein

4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Gene

ispH

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.UniRule annotation

Catalytic activityi

Isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation
Dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: dimethylallyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway dimethylallyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate, the pathway dimethylallyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 6 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Bifunctional enzyme IspD/IspF (ispDF)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. Bifunctional enzyme IspD/IspF (ispDF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi19Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei48SubstrateUniRule annotation1
Binding sitei84SubstrateUniRule annotation1
Metal bindingi106Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei134SubstrateUniRule annotation1
Active sitei136Proton donorUniRule annotation1
Binding sitei175SubstrateUniRule annotation1
Metal bindingi205Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei278SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processIsoprene biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciBMEL224914:G1FZL-1569-MONOMER
UniPathwayiUPA00056; UER00097
UPA00059; UER00105

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductaseUniRule annotation (EC:1.17.7.4UniRule annotation)
Short name:
HMBPP reductaseUniRule annotation
Gene namesi
Name:ispHUniRule annotation
Synonyms:lytB
Ordered Locus Names:BMEI1459
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000419 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001287871 – 3464-hydroxy-3-methylbut-2-enyl diphosphate reductaseAdd BLAST346

Proteomic databases

PRIDEiQ8YFR1

Interactioni

Protein-protein interaction databases

STRINGi224914.BAWG_0764

Structurei

3D structure databases

SMRiQ8YFR1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni233 – 235Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the IspH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C48 Bacteria
COG0761 LUCA
HOGENOMiHOG000220192
KOiK03527
OMAiHNKYVVD

Family and domain databases

CDDicd13944 lytB_ispH, 1 hit
HAMAPiMF_00191 IspH, 1 hit
InterProiView protein in InterPro
IPR003451 LytB/IspH
PANTHERiPTHR30426 PTHR30426, 1 hit
PfamiView protein in Pfam
PF02401 LYTB, 1 hit
TIGRFAMsiTIGR00216 ispH_lytB, 1 hit

Sequencei

Sequence statusi: Complete.

Q8YFR1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQHRPPLEI RLCGPRGFCA GVDRAIQIVV LALKKYGAPV YVRHEIVHNR
60 70 80 90 100
YVVEGLQARG AIFVEELDEI PAAHRNQPVV FSAHGVPKSV PADAEAKNLF
110 120 130 140 150
YLDATCPLVS KVHKQAMRHQ RLGRHVILIG HSGHPEVIGT MGQLPDGAVT
160 170 180 190 200
LIETVEDAHT CHFDDEDNLG FVTQTTLSVD DTAGIIKELQ ARFPNLAAPA
210 220 230 240 250
AESICYATTN RQDAVRAAAP GCDLFLIVGA PNSSNSKRLV EVAEKAGARM
260 270 280 290 300
SMLVQRAEDI EWEQIGDISV VGLSAGASAP EIIVDEIIDA FKARFDVKIE
310 320 330 340
LAETTVETEN FLVNREIRDV ELTVKDMAFV NGEHRVVGIS KLMQGK
Length:346
Mass (Da):37,747
Last modified:March 5, 2002 - v1
Checksum:iEB9C1D60EF73421B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA Translation: AAL52640.1
PIRiAE3434
RefSeqiWP_004683139.1, NZ_GG703778.1

Genome annotation databases

EnsemblBacteriaiAAL52640; AAL52640; BMEI1459
GeneIDi29594309
KEGGibme:BMEI1459
bmel:DK63_2028
PATRICifig|224914.52.peg.2130

Similar proteinsi

Entry informationi

Entry nameiISPH_BRUME
AccessioniPrimary (citable) accession number: Q8YFR1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 5, 2002
Last modified: May 23, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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