Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Argininosuccinate synthase

Gene

argG

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei91CitrullineUniRule annotation1
Binding sitei96CitrullineUniRule annotation1
Binding sitei121ATP; via amide nitrogenUniRule annotation1
Binding sitei123AspartateUniRule annotation1
Binding sitei127AspartateUniRule annotation1
Binding sitei127CitrullineUniRule annotation1
Binding sitei128AspartateUniRule annotation1
Binding sitei131CitrullineUniRule annotation1
Binding sitei182CitrullineUniRule annotation1
Binding sitei191CitrullineUniRule annotation1
Binding sitei267CitrullineUniRule annotation1
Binding sitei279CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 21ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:BMEI1870
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000419 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001485741 – 406Argininosuccinate synthaseAdd BLAST406

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi224914.BAWG_0369

Structurei

3D structure databases

ProteinModelPortaliQ8YEK8
SMRiQ8YEK8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDH Bacteria
COG0137 LUCA
HOGENOMiHOG000230093
KOiK01940
OMAiGVGRIDM

Family and domain databases

CDDicd01999 Argininosuccinate_Synthase, 1 hit
Gene3Di3.40.50.620, 1 hit
3.90.1260.10, 1 hit
HAMAPiMF_00005 Arg_succ_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001518 Arginosuc_synth
IPR018223 Arginosuc_synth_CS
IPR023434 Arginosuc_synth_type_1_subfam
IPR024074 AS_cat/multimer_dom_body
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR11587 PTHR11587, 1 hit
PfamiView protein in Pfam
PF00764 Arginosuc_synth, 1 hit
TIGRFAMsiTIGR00032 argG, 1 hit
PROSITEiView protein in PROSITE
PS00564 ARGININOSUCCIN_SYN_1, 1 hit
PS00565 ARGININOSUCCIN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8YEK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKWKDVKKV VLAYSGGLDT SIILKWLQTE LGAEVVTFTA DLGQGEELEP
60 70 80 90 100
ARKKAEMLGI KEIFIEDVRE EFVRDFVFPM FRANAVYEGV YLLGTSIARP
110 120 130 140 150
LISKHLIDIA KKTGADAIAH GATGKGNDQV RFELSAYALN PDIKIIAPWR
160 170 180 190 200
DWSFKSRTQL LEFAEQHQIP VAKDKKGEAP FSVDANLLHS SSEGKVLEDP
210 220 230 240 250
SQEAPEYVHM RTISPETAPD KATIIKIGFE KGDAVSINGE RLSPATLLAK
260 270 280 290 300
LNDYGRDNGI GRLDLVENRF VGMKSRGVYE TPGGTILLAA HRAIESITLD
310 320 330 340 350
RGAAHLKDEL MPRYAELIYY GFWFSPEREM LQAAIDHSQR HVEGEVTLKL
360 370 380 390 400
YKGNVMVIGR ESAKSLYSDK LVTFEDDQGA YDQKDAAGFI KLNALRLRTL

AARDRK
Length:406
Mass (Da):45,273
Last modified:March 1, 2002 - v1
Checksum:i534F07859F1B8F7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA Translation: AAL53051.1
PIRiAH3485
RefSeqiWP_002965322.1, NZ_GG703778.1

Genome annotation databases

EnsemblBacteriaiAAL53051; AAL53051; BMEI1870
GeneIDi29594749
KEGGibme:BMEI1870
bmel:DK63_1618
PATRICifig|224914.52.peg.1707

Similar proteinsi

Entry informationi

Entry nameiASSY_BRUME
AccessioniPrimary (citable) accession number: Q8YEK8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: March 1, 2002
Last modified: June 7, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health