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Entry version 116 (25 May 2022)
Sequence version 1 (01 Mar 2002)
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Protein

Bifunctional protein TilS/HprT

Gene

tilS/hprT

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine (By similarity).

By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. One of the ions does not make direct protein contacts.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi627Magnesium 1By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi29 – 34ATPSequence analysis6

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGlycosyltransferase, Ligase, Transferase
Biological processtRNA processing
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Bifunctional protein TilS/HprT
Including the following 2 domains:
tRNA(Ile)-lysidine synthase (EC:6.3.4.19)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthase
tRNA(Ile)-lysidine synthetase
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:tilS/hprT
Ordered Locus Names:lmo0219
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri169963 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000817 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001817181 – 648Bifunctional protein TilS/HprTAdd BLAST648

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q8YAC7

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
169963.lmo0219

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

AlphaFold Protein Structure Database

More...
AlphaFoldDBi
Q8YAC7

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q8YAC7

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

In the N-terminal section; belongs to the tRNA(Ile)-lysidine synthase family.Curated
In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG0037, Bacteria
COG0634, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_018869_0_1_9

Identification of Orthologs from Complete Genome Data

More...
OMAi
QQYGMNA

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q8YAC7

Family and domain databases

Conserved Domains Database

More...
CDDi
cd01992, PP-ATPase, 1 hit
cd06223, PRTases_typeI, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.50.2020, 1 hit
3.40.50.620, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_01161, tRNA_Ile_lys_synt, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR005904, Hxn_phspho_trans
IPR012796, Lysidine-tRNA-synth_C
IPR000836, PRibTrfase_dom
IPR029057, PRTase-like
IPR014729, Rossmann-like_a/b/a_fold
IPR011063, TilS/TtcA_N
IPR012094, tRNA_Ile_lys_synt
IPR012795, tRNA_Ile_lys_synt_N

The PANTHER Classification System

More...
PANTHERi
PTHR43033, PTHR43033, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01171, ATP_bind_3, 1 hit
PF00156, Pribosyltran, 1 hit
PF11734, TilS_C, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00977, TilS_C, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF53271, SSF53271, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01203, HGPRTase, 1 hit
TIGR02433, lysidine_TilS_C, 1 hit
TIGR02432, lysidine_TilS_N, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00103, PUR_PYR_PR_TRANSFER, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q8YAC7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDDTEKRVHK YIEKHDLIRS DDKLLVAVSG GPDSLALLHF LWNSNLVPKE
60 70 80 90 100
AISVAHLNHQ LRENAAKEQR VVETFCERQG IPFYIEEVDI KSRAQSLQKG
110 120 130 140 150
LEETARIVRY DFFEKVMAEK NINKLVLAHH ADDQIETILM RLVRGSASIG
160 170 180 190 200
WSGIQPKREL KGGQAIRPFL PITKAEIIDY AQKHELAYEI DESNTSQEYT
210 220 230 240 250
RNRYRAQLLP FLKQENPAVY SHFERFSEET SEDFQFLEAL ASDLLKKNLI
260 270 280 290 300
KNGKQTTLLL SSFKNEANPL QRRAIHLLLR YLYNEDASFI TVNHIYQIIQ
310 320 330 340 350
MIQSDNPSSS IDLPNKLIAN RAYDKLHFQF GEREAPSEFY HQLELNDRIE
360 370 380 390 400
LDNKASIRLK LKSSVVQTNG LNGMLLDAEE ITLPLIVRNR VNGDRMTMKG
410 420 430 440 450
QAGSKKLKDI FIDAKIPRQE RDKLPVITDY TGKILWVPGV KKSAYDREFS
460 470 480 490 500
RSKKQYIIRY TRNIGGNESM HNDIQKVLIS EDELQEKIRE LGRELTTEYE
510 520 530 540 550
GRNPLVVGVL KGATPFMTDL LKRVDTYLEM DFMDVSSYGN GTVSSGEVKI
560 570 580 590 600
IKDLNASVEG RDVLVIEDII DSGRTLSYLV DLIKYRKAKS VKLVTLLDKP
610 620 630 640
AGRNVEIEAD YVGFVVPNEF VVGYGLDYAE RYRNLPYIGI LKPEIYSE
Length:648
Mass (Da):74,368
Last modified:March 1, 2002 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iDACEA788D7AD5F77
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti5E → D in AAM74001 (Ref. 2) Curated1
Sequence conflicti57L → I in AAM74001 (Ref. 2) Curated1
Sequence conflicti60Q → H in AAM74001 (Ref. 2) Curated1
Sequence conflicti67K → N in AAM74001 (Ref. 2) Curated1
Sequence conflicti70R → N in AAM74001 (Ref. 2) Curated1
Sequence conflicti74T → A in AAM74001 (Ref. 2) Curated1
Sequence conflicti78R → S in AAM74001 (Ref. 2) Curated1
Sequence conflicti90I → V in AAM74001 (Ref. 2) Curated1
Sequence conflicti95Q → E in AAM74001 (Ref. 2) Curated1
Sequence conflicti101L → I in AAM74001 (Ref. 2) Curated1
Sequence conflicti121N → K in AAM74001 (Ref. 2) Curated1
Sequence conflicti126V → A in AAM74001 (Ref. 2) Curated1
Sequence conflicti255Q → R in AAM74001 (Ref. 2) Curated1
Sequence conflicti261 – 262SS → TN in AAM74001 (Ref. 2) Curated2
Sequence conflicti318I → V in AAM74001 (Ref. 2) Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AL591974 Genomic DNA Translation: CAD00746.1
AF467001 Genomic DNA Translation: AAM74001.1

Protein sequence database of the Protein Information Resource

More...
PIRi
AD1102

NCBI Reference Sequences

More...
RefSeqi
NP_463750.1, NC_003210.1
WP_003723741.1, NZ_CP023861.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
CAD00746; CAD00746; CAD00746

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
987055

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
lmo:lmo0219

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|169963.11.peg.224

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591974 Genomic DNA Translation: CAD00746.1
AF467001 Genomic DNA Translation: AAM74001.1
PIRiAD1102
RefSeqiNP_463750.1, NC_003210.1
WP_003723741.1, NZ_CP023861.1

3D structure databases

AlphaFoldDBiQ8YAC7
SMRiQ8YAC7
ModBaseiSearch...

Protein-protein interaction databases

STRINGi169963.lmo0219

Proteomic databases

PaxDbiQ8YAC7

Genome annotation databases

EnsemblBacteriaiCAD00746; CAD00746; CAD00746
GeneIDi987055
KEGGilmo:lmo0219
PATRICifig|169963.11.peg.224

Phylogenomic databases

eggNOGiCOG0037, Bacteria
COG0634, Bacteria
HOGENOMiCLU_018869_0_1_9
OMAiQQYGMNA
PhylomeDBiQ8YAC7

Family and domain databases

CDDicd01992, PP-ATPase, 1 hit
cd06223, PRTases_typeI, 1 hit
Gene3Di3.40.50.2020, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_01161, tRNA_Ile_lys_synt, 1 hit
InterProiView protein in InterPro
IPR005904, Hxn_phspho_trans
IPR012796, Lysidine-tRNA-synth_C
IPR000836, PRibTrfase_dom
IPR029057, PRTase-like
IPR014729, Rossmann-like_a/b/a_fold
IPR011063, TilS/TtcA_N
IPR012094, tRNA_Ile_lys_synt
IPR012795, tRNA_Ile_lys_synt_N
PANTHERiPTHR43033, PTHR43033, 1 hit
PfamiView protein in Pfam
PF01171, ATP_bind_3, 1 hit
PF00156, Pribosyltran, 1 hit
PF11734, TilS_C, 1 hit
SMARTiView protein in SMART
SM00977, TilS_C, 1 hit
SUPFAMiSSF53271, SSF53271, 1 hit
TIGRFAMsiTIGR01203, HGPRTase, 1 hit
TIGR02433, lysidine_TilS_C, 1 hit
TIGR02432, lysidine_TilS_N, 1 hit
PROSITEiView protein in PROSITE
PS00103, PUR_PYR_PR_TRANSFER, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTILS_LISMO
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8YAC7
Secondary accession number(s): Q8KU03
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 1, 2002
Last modified: May 25, 2022
This is version 116 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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