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Protein

S-adenosylmethionine synthase

Gene

metK

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.UniRule annotation

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 divalent ions per subunit.UniRule annotation
  • K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase (metK)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei17ATPUniRule annotation1
Metal bindingi19MagnesiumUniRule annotation1
Metal bindingi45PotassiumUniRule annotation1
Binding sitei58MethionineUniRule annotation1
Binding sitei101MethionineUniRule annotation1
Binding sitei253ATP; shared with neighboring subunitUniRule annotation1
Binding sitei253Methionine; shared with neighboring subunitUniRule annotation1
Binding sitei276ATP; via amide nitrogen; shared with neighboring subunitUniRule annotation1
Binding sitei280ATP; shared with neighboring subunitUniRule annotation1
Binding sitei284MethionineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi177 – 179ATPUniRule annotation3
Nucleotide bindingi244 – 245ATPUniRule annotation2
Nucleotide bindingi259 – 260ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processOne-carbon metabolism
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciLMON169963:LMO1664-MONOMER
UniPathwayiUPA00315; UER00080

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthaseUniRule annotation (EC:2.5.1.6UniRule annotation)
Short name:
AdoMet synthaseUniRule annotation
Alternative name(s):
MATUniRule annotation
Methionine adenosyltransferaseUniRule annotation
Gene namesi
Name:metKUniRule annotation
Ordered Locus Names:lmo1664
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000000817 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001745451 – 399S-adenosylmethionine synthaseAdd BLAST399

Proteomic databases

PaxDbiQ8Y6M0

Interactioni

Subunit structurei

Homotetramer; dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi169963.lmo1664

Structurei

3D structure databases

ProteinModelPortaliQ8Y6M0
SMRiQ8Y6M0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni101 – 111Flexible loopUniRule annotationAdd BLAST11

Sequence similaritiesi

Belongs to the AdoMet synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPH Bacteria
COG0192 LUCA
HOGENOMiHOG000245710
KOiK00789
OMAiPGHFLFT
PhylomeDBiQ8Y6M0

Family and domain databases

HAMAPiMF_00086 S_AdoMet_synth1, 1 hit
InterProiView protein in InterPro
IPR022631 ADOMET_SYNTHASE_CS
IPR022630 S-AdoMet_synt_C
IPR022629 S-AdoMet_synt_central
IPR022628 S-AdoMet_synt_N
IPR002133 S-AdoMet_synthetase
IPR022636 S-AdoMet_synthetase_sfam
PANTHERiPTHR11964 PTHR11964, 1 hit
PfamiView protein in Pfam
PF02773 S-AdoMet_synt_C, 1 hit
PF02772 S-AdoMet_synt_M, 1 hit
PF00438 S-AdoMet_synt_N, 1 hit
PIRSFiPIRSF000497 MAT, 1 hit
SUPFAMiSSF55973 SSF55973, 3 hits
TIGRFAMsiTIGR01034 metK, 1 hit
PROSITEiView protein in PROSITE
PS00376 ADOMET_SYNTHASE_1, 1 hit
PS00377 ADOMET_SYNTHASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8Y6M0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKNRHLFTS ESVSDGHPDK IADQISDAIL DAIISKDPDA RVACETTVTT
60 70 80 90 100
GLVLVAGEIT TSVYVDIPKI VRDTIKEIGY TRAKYGFDAE TCAVLTAIDE
110 120 130 140 150
QSPDIAQGVD EALESRSGNE IDAAIEAIGA GDQGLMFGFA TDETEELMPL
160 170 180 190 200
PIFLAHGLAR KLTELRKTNK LDYLRPDAKT QVTVEYDEFN QPVRIDTIVV
210 220 230 240 250
STQHHPDITQ EQIAKDLHTY LFPEVIDASF LDEDTKYFIN PTGRFVIGGP
260 270 280 290 300
LGDAGLTGRK IIVDTYGGYA RHGGGAFSGK DPTKVDRSGA YAARYVAKNI
310 320 330 340 350
VAAGLAKKVE VQVAYAIGVA RPVSISIDTY GTSDYSEQEL IDGVNALFDL
360 370 380 390
RPAGIIHMLD LRRPIYRQTA AFGHFGRSDL DLPWERTDKA EALKKLIVK
Length:399
Mass (Da):43,551
Last modified:March 1, 2002 - v1
Checksum:i17A473504AE2504D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591980 Genomic DNA Translation: CAC99742.1
PIRiAH1282
RefSeqiNP_465189.1, NC_003210.1
WP_003733830.1, NC_003210.1

Genome annotation databases

EnsemblBacteriaiCAC99742; CAC99742; CAC99742
GeneIDi985664
KEGGilmo:lmo1664
PATRICifig|169963.11.peg.1707

Similar proteinsi

Entry informationi

Entry nameiMETK_LISMO
AccessioniPrimary (citable) accession number: Q8Y6M0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: March 1, 2002
Last modified: June 20, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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