UniProtKB - Q8XBV9 (ENTF_ECO57)
Enterobactin synthase component F
entF
Functioni
Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine. EntF catalyzes the activation of L-serine via ATP-dependent PPi exchange reaction to form seryladenylate. Activated L-serine is loaded onto the peptidyl carrier domain via a thioester linkage to the phosphopanthetheine moiety, forming seryl-S-Ppant-EntF. EntF acts then as the sole catalyst for the formation of the three amide and three ester linkages found in enterobactin, using seryladenylate and 2,3-dihydroxybenzoate-S-Ppant-EntB (DHB-S-Ppant-EntB) as substrates, via the formation of a DHB-Ser-S-Ppant-EntF intermediate.
By similarityCatalytic activityi
- 3 2,3-dihydroxybenzoate + 6 ATP + 3 L-serine = 6 AMP + 6 diphosphate + enterobactin + 4 H+By similarityEC:6.3.2.14By similarity
- ATP + holo-[peptidyl-carrier protein] + L-serine = AMP + diphosphate + L-seryl-[peptidyl-carrier protein]By similarityEC:6.2.1.72By similarity
Cofactori
: enterobactin biosynthesis Pathwayi
This protein is involved in the pathway enterobactin biosynthesis, which is part of Siderophore biosynthesis.By similarityView all proteins of this organism that are known to be involved in the pathway enterobactin biosynthesis and in Siderophore biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 1271 | Proton acceptor; for thioesterase activityBy similarity | 1 |
GO - Molecular functioni
- 2,3-dihydroxybenzoate-serine ligase activity Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- nucleotidyltransferase activity Source: UniProtKB
- phosphopantetheine binding Source: UniProtKB
GO - Biological processi
- amino acid activation for nonribosomal peptide biosynthetic process Source: UniProtKB
- enterobactin biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Ligase, Multifunctional enzyme, Transferase |
Biological process | Enterobactin biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00017 |
Protein family/group databases
ESTHERi | ecoli-entf, Thioesterase |
Names & Taxonomyi
Protein namesi | Recommended name: Enterobactin synthase component FBy similarity (EC:6.3.2.14By similarity)Alternative name(s): Enterochelin synthase FBy similarity Nonribosomal peptide synthetase EntFBy similarity Including the following 1 domains: Alternative name(s): Serine-activating enzymeBy similarity Seryl-AMP ligaseBy similarity |
Gene namesi | Name:entF Ordered Locus Names:Z0727, ECs0625 |
Organismi | Escherichia coli O157:H7 |
Taxonomic identifieri | 83334 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Note: Membrane-associated.By similarity
Cytosol
- cytosol Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- enterobactin synthetase complex Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193078 | 1 – 1293 | Enterobactin synthase component FAdd BLAST | 1293 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1006 | O-(pantetheine 4'-phosphoryl)serineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphopantetheine, PhosphoproteinInteractioni
Subunit structurei
Proteins EntB, EntD, EntE and EntF are the component of the enterobactin synthase. Components probably do not form a stable complex. EntF acts as a catalytic monomer.
By similarityProtein-protein interaction databases
STRINGi | 155864.EDL933_0656 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 971 – 1046 | CarrierPROSITE-ProRule annotationAdd BLAST | 76 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 301 | Elongation/condensationCuratedAdd BLAST | 301 | |
Regioni | 482 – 887 | AdenylationCuratedAdd BLAST | 406 | |
Regioni | 1066 – 1293 | ThioesteraseCuratedAdd BLAST | 228 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1020, Bacteria COG3319, Bacteria |
HOGENOMi | CLU_000022_2_13_6 |
OMAi | IISPQAF |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.30.559.10, 1 hit 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR010071, AA_adenyl_domain IPR029058, AB_hydrolase IPR036736, ACP-like_sf IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR023213, CAT-like_dom_sf IPR001242, Condensatn IPR020806, PKS_PP-bd IPR020802, PKS_thioesterase IPR009081, PP-bd_ACP IPR006162, Ppantetheine_attach_site IPR001031, Thioesterase |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF00668, Condensation, 1 hit PF00550, PP-binding, 1 hit PF00975, Thioesterase, 1 hit |
SMARTi | View protein in SMART SM00823, PKS_PP, 1 hit SM00824, PKS_TE, 1 hit |
SUPFAMi | SSF47336, SSF47336, 1 hit SSF53474, SSF53474, 1 hit |
TIGRFAMsi | TIGR01733, AA-adenyl-dom, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit PS50075, CARRIER, 1 hit PS00012, PHOSPHOPANTETHEINE, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSQHLPLVAA QPGIWMAEKL SELPSAWSVA HYVELTGEVD APLLARAVVA
60 70 80 90 100
GLAQADTLRM RFTEDNGEVW QWVDDALIFE LPEIIDLRTN IDPHGTAQAL
110 120 130 140 150
MQADLQQDLR VDSGKPLVFH QLIQVADNRW YWYQRYHHLL VDGFSFPAIT
160 170 180 190 200
RQIANIYCAL LRGEQTPASP FTPFADVVEE YQQYRESEAW QRDAAFWAEQ
210 220 230 240 250
RRQLPPPASL SPAPLAGRSA SADILRLKLE FTDGEFRQLA TQLSGVQRTD
260 270 280 290 300
LALALAAFWL GRLCNRMDYA AGFIFMRRLG SAALTATGPV LNVLPLGIHI
310 320 330 340 350
AAQETLPELA TRLAAQLKKM RRHQRYDAEQ IVRDSGRAAG DEPLFGPVLN
360 370 380 390 400
IKVFDYQLDI PGVQAQTHTL ATGPVNDLEL ALFPDEHGDL SIEILANKQH
410 420 430 440 450
YDEPTLIQHA ERLKMLIAQF AADPALLCGD VDIMLPGEYA QLAQINATQV
460 470 480 490 500
EIPETTLSAL VAEQAAKTPD APALADARYQ FSYREMREQV VALANLLREH
510 520 530 540 550
GVKPGDSVAV ALPRSVFLTL ALHAIVEAGA AWLPLDTGYP DDRLKMMLED
560 570 580 590 600
ARPSLLITTD DQLPRFADVP DLTNLCYNAP LTPQGSAPLQ LSQPHHTAYI
610 620 630 640 650
IFTSGSTGRP KGVMVGQTAI VNRLLWMQNH YPLTGEDVVA QKTPCSFDVS
660 670 680 690 700
VWEFFWPFIA GAKLVMAEPE AHRDPLAMQQ FFAEYGVTTT HFVPSMLAAF
710 720 730 740 750
VASLTPQTAR QNCATLKQVF CSGEALPADL CREWQQLTGA PLHNLYGPTE
760 770 780 790 800
AAVDVSWYPA FGEELAQVRG SSVPIGYPVW NTGLRILDAM MHPVPPGVAG
810 820 830 840 850
DLYLTGIQLA QGYLGRPDLT ASRFIADPFV PGERMYRTGD VARWLDNGAV
860 870 880 890 900
EYLGRSDDQL KIRGQRIELG EIDRVMQALP DVEQAVTHAC VINQAAATGG
910 920 930 940 950
DARQLVGYLV SQSGLPLDTS ALQAQLRETL PPHMAPVVLL QLPQLPLSAN
960 970 980 990 1000
GKLDRKALPL PELKAQTPGR APKAGSETII AAAFASLLGC DVQDADADFF
1010 1020 1030 1040 1050
ALGGHSLLAM KLAAQLSRQF ARQVTPGQVM VASTVAKLAT IIDGEEDSSR
1060 1070 1080 1090 1100
RMGFETILPL REGNGPTLFC FHPASGFAWQ FSVLSRYLDP LWSIIGIQSP
1110 1120 1130 1140 1150
RPHGPMQTAT NLDEVCEAHL ATLLEQQPHG PYYLLGYSLG GTLAQGIAAR
1160 1170 1180 1190 1200
LRARGEQVAF LGLLDTWPPE TQNWQEKEAN GLDPEVLAEI NREREAFLAA
1210 1220 1230 1240 1250
QQGSTSTELF TTIEGNYADA VRLLTTAHSV PFDGKATLFV AERTLQEGMS
1260 1270 1280 1290
PERAWSPWIA ELDIYRQDCA HVDIISPGAF EKIGPIIRAT LNR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005174 Genomic DNA Translation: AAG54921.1 BA000007 Genomic DNA Translation: BAB34048.1 |
PIRi | A90707 E85557 |
RefSeqi | NP_308652.1, NC_002695.1 WP_000077784.1, NZ_SEKU01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAG54921; AAG54921; Z0727 BAB34048; BAB34048; ECs_0625 |
GeneIDi | 916984 |
KEGGi | ece:Z0727 ecs:ECs0625 |
PATRICi | fig|386585.9.peg.735 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005174 Genomic DNA Translation: AAG54921.1 BA000007 Genomic DNA Translation: BAB34048.1 |
PIRi | A90707 E85557 |
RefSeqi | NP_308652.1, NC_002695.1 WP_000077784.1, NZ_SEKU01000009.1 |
3D structure databases
AlphaFoldDBi | Q8XBV9 |
SMRi | Q8XBV9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 155864.EDL933_0656 |
Protein family/group databases
ESTHERi | ecoli-entf, Thioesterase |
Genome annotation databases
EnsemblBacteriai | AAG54921; AAG54921; Z0727 BAB34048; BAB34048; ECs_0625 |
GeneIDi | 916984 |
KEGGi | ece:Z0727 ecs:ECs0625 |
PATRICi | fig|386585.9.peg.735 |
Phylogenomic databases
eggNOGi | COG1020, Bacteria COG3319, Bacteria |
HOGENOMi | CLU_000022_2_13_6 |
OMAi | IISPQAF |
Enzyme and pathway databases
UniPathwayi | UPA00017 |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.30.559.10, 1 hit 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR010071, AA_adenyl_domain IPR029058, AB_hydrolase IPR036736, ACP-like_sf IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR023213, CAT-like_dom_sf IPR001242, Condensatn IPR020806, PKS_PP-bd IPR020802, PKS_thioesterase IPR009081, PP-bd_ACP IPR006162, Ppantetheine_attach_site IPR001031, Thioesterase |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF00668, Condensation, 1 hit PF00550, PP-binding, 1 hit PF00975, Thioesterase, 1 hit |
SMARTi | View protein in SMART SM00823, PKS_PP, 1 hit SM00824, PKS_TE, 1 hit |
SUPFAMi | SSF47336, SSF47336, 1 hit SSF53474, SSF53474, 1 hit |
TIGRFAMsi | TIGR01733, AA-adenyl-dom, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit PS50075, CARRIER, 1 hit PS00012, PHOSPHOPANTETHEINE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ENTF_ECO57 | |
Accessioni | Q8XBV9Primary (citable) accession number: Q8XBV9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 26, 2002 |
Last sequence update: | March 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families