UniProtKB - Q8XBV3 (ENTE_ECO57)
Enterobactin synthase component E
entE
Functioni
Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine. The serine trilactone serves as a scaffolding for the three catechol functionalities that provide hexadentate coordination for the tightly ligated iron2+ atoms. EntE proccesses via a two-step adenylation-ligation reaction (bi-uni-uni-bi ping-pong mechanism). First, it catalyzes the activation of the carboxylate group of 2,3-dihydroxy-benzoate (DHB), via a reversible ATP-dependent pyrophosphate exchange reactions to yield the acyladenylate intermediate 2,3-dihydroxybenzoyl-AMP. It can also transfer AMP to salicylate, 2,4-dihydroxybenzoate, gentisate and 2,3,4-trihydroxybenzoate. In the second step, DHB is transferred from 2,3-dihydroxybenzoyl-AMP onto the phosphopantetheinylated EntB (holo-EntB) to form DHB-holo-EntB. Then this product will serve in the formation of the amide bond between 2,3-dihydroxybenzoate (DHB) and L-serine.
By similarityCatalytic activityi
- 3 2,3-dihydroxybenzoate + 6 ATP + 3 L-serine = 6 AMP + 6 diphosphate + enterobactin + 4 H+By similarityEC:6.3.2.14By similarity
- 2,3-dihydroxybenzoate + ATP + holo-[aryl-carrier protein] = 2,3-dihydroxybenzoyl-[aryl-carrier protein] + AMP + diphosphateBy similarityEC:6.2.1.71By similarity
: enterobactin biosynthesis Pathwayi
This protein is involved in the pathway enterobactin biosynthesis, which is part of Siderophore biosynthesis.By similarityView all proteins of this organism that are known to be involved in the pathway enterobactin biosynthesis and in Siderophore biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 235 | SubstrateBy similarity | 1 | |
Binding sitei | 240 | SubstrateBy similarity | 1 | |
Binding sitei | 309 | Substrate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 331 | Substrate; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 335 | Substrate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 415 | SubstrateBy similarity | 1 | |
Binding sitei | 432 | SubstrateBy similarity | 1 | |
Binding sitei | 441 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- (2,3-dihydroxybenzoyl)adenylate synthase activity Source: UniProtKB
- 2,3-dihydroxybenzoate-serine ligase activity Source: UniProtKB
- acyltransferase activity Source: UniProtKB-KW
- ATP binding Source: UniProtKB-KW
GO - Biological processi
- enterobactin biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Ligase, Transferase |
Biological process | Enterobactin biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00017 |
Names & Taxonomyi
Protein namesi | Recommended name: Enterobactin synthase component EBy similarity (EC:6.3.2.14By similarity)Alternative name(s): 2,3-dihydroxybenzoate-AMP liaseBy similarity Short name: DHB-AMP ligaseBy similarity 2,3-dihydroxybenzoate-AMP synthaseBy similarity (EC:6.2.1.71By similarity) Dihydroxybenzoic acid-activating enzymeBy similarity Enterochelin synthase EBy similarity |
Gene namesi | Name:entEBy similarity Ordered Locus Names:Z0736, ECs0633 |
Organismi | Escherichia coli O157:H7 |
Taxonomic identifieri | 83334 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Membrane By similarity
Other locations
- membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193076 | 1 – 536 | Enterobactin synthase component EAdd BLAST | 536 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Proteins EntB, EntD, EntE, and EntF form a multienzyme complex called enterobactin synthase. Monomer. EntA and EntE interact together.
By similarityProtein-protein interaction databases
STRINGi | 386585.gene:10363621 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 438 – 439 | Phosphopantetheine bindingBy similarity | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1021, Bacteria |
HOGENOMi | CLU_000022_59_7_6 |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf IPR011963, DHB_AMP_lig |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
TIGRFAMsi | TIGR02275, DHB_AMP_lig, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSIPFTRWPE EFARRYREKG YWQDLPLTDI LTRHAASDSI AVIDGERQLS
60 70 80 90 100
YRELNQAADN LACSLRRQGI KPGETALVQL GNVAELYITF FALLKLGVAP
110 120 130 140 150
VLALFSHQRS ELNAYASQIE PALLIADRQH ALFSGDDFLN TFVAEHSSIR
160 170 180 190 200
VVQLLNDSGE HNLQDAINHP ADGFTATPSP ADEVVYFQLS GGTTGTPKLI
210 220 230 240 250
PRTHNDYYYS VRRSVEICQF TQQTRYLCAI PAAHNYAMSS PGSLGVFLAG
260 270 280 290 300
GTVVLAADPS ATLCFPLIEK HQINVTALVP PAVSLWLQAL TEGESRAQLA
310 320 330 340 350
SLKLLQVGGA RLSATLAARI PAEIGCQLQQ VFGMAEGLVN YTRLDDSAEK
360 370 380 390 400
IIHTQGYPMC PDDEVWVADA EGNPLPQGEV GRLMTRGPYT FRGYYKSPQH
410 420 430 440 450
NASAFDANGF YCSGDLISID PEGYITVQGR EKDQINRGGE KIAAEEIENL
460 470 480 490 500
LLRHPAVIYA ALVSMEDELM GEKSCAYLVV KEPLRAVQVR RFLREQGIAE
510 520 530
FKLPDRVECV DSLPLTAVGK VDKKQLRQWL ASRASA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005174 Genomic DNA Translation: AAG54929.1 BA000007 Genomic DNA Translation: BAB34056.1 |
PIRi | A99708 E85558 |
RefSeqi | NP_308660.1, NC_002695.1 WP_000026781.1, NZ_SEKU01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAG54929; AAG54929; Z0736 BAB34056; BAB34056; ECs_0633 |
GeneIDi | 916992 |
KEGGi | ece:Z0736 ecs:ECs0633 |
PATRICi | fig|386585.9.peg.743 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005174 Genomic DNA Translation: AAG54929.1 BA000007 Genomic DNA Translation: BAB34056.1 |
PIRi | A99708 E85558 |
RefSeqi | NP_308660.1, NC_002695.1 WP_000026781.1, NZ_SEKU01000009.1 |
3D structure databases
SMRi | Q8XBV3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 386585.gene:10363621 |
Genome annotation databases
EnsemblBacteriai | AAG54929; AAG54929; Z0736 BAB34056; BAB34056; ECs_0633 |
GeneIDi | 916992 |
KEGGi | ece:Z0736 ecs:ECs0633 |
PATRICi | fig|386585.9.peg.743 |
Phylogenomic databases
eggNOGi | COG1021, Bacteria |
HOGENOMi | CLU_000022_59_7_6 |
Enzyme and pathway databases
UniPathwayi | UPA00017 |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf IPR011963, DHB_AMP_lig |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
TIGRFAMsi | TIGR02275, DHB_AMP_lig, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ENTE_ECO57 | |
Accessioni | Q8XBV3Primary (citable) accession number: Q8XBV3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 26, 2002 |
Last sequence update: | March 1, 2002 | |
Last modified: | February 23, 2022 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families