UniProtKB - Q8XBQ8 (PTFX_ECO57)
Protein
Multiphosphoryl transfer protein
Gene
fryA
Organism
Escherichia coli O157:H7
Status
Functioni
Multifunctional protein that includes general (non sugar-specific) and sugar-specific components of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II FryABC PTS system is involved in fructose transport.By similarity
Catalytic activityi
- L-histidyl-[protein] + phosphoenolpyruvate = Nπ-phospho-L-histidyl-[protein] + pyruvateBy similarityEC:2.7.3.9By similarity
- D-fructose(out) + Nπ-phospho-L-histidyl-[protein] = D-fructose 1-phosphate(in) + L-histidyl-[protein]By similarityEC:2.7.1.202By similarity
Cofactori
Mg2+By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 15 | Pros-phosphohistidine intermediate; for HPr activityPROSITE-ProRule annotation | 1 | |
Active sitei | 298 | Tele-phosphohistidine intermediate; for PTS EI activityPROSITE-ProRule annotationBy similarity | 1 | |
Binding sitei | 405 | PEPBy similarity | 1 | |
Binding sitei | 441 | PEPBy similarity | 1 | |
Metal bindingi | 540 | MagnesiumBy similarity | 1 | |
Metal bindingi | 564 | MagnesiumBy similarity | 1 | |
Binding sitei | 574 | PEPBy similarity | 1 | |
Active sitei | 611 | Proton donor; for EI activityBy similarity | 1 | |
Active sitei | 747 | Tele-phosphohistidine intermediate; for PTS EIIA activityPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- kinase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- phosphoenolpyruvate-protein phosphotransferase activity Source: UniProtKB-EC
- protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Source: InterPro
GO - Biological processi
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Phosphotransferase system, Sugar transport, Transport |
Ligand | Magnesium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Multiphosphoryl transfer proteinBy similarityShort name: MTPBy similarity Alternative name(s): Triphosphoryl transfer proteinBy similarity Short name: TTPBy similarity Including the following 3 domains: Alternative name(s): Phosphotransferase system enzyme IBy similarity Phosphocarrier protein HPrBy similarity Short name: Protein HBy similarity Alternative name(s): Fructose-like phosphotransferase enzyme IIA componentBy similarity |
Gene namesi | Name:fryA Ordered Locus Names:Z3648, ECs3263 |
Organismi | Escherichia coli O157:H7 |
Taxonomic identifieri | 83334 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
- integral component of membrane Source: InterPro
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000147099 | 1 – 831 | Multiphosphoryl transfer proteinAdd BLAST | 831 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 15 | Phosphohistidine; by EICurated | 1 | |
Modified residuei | 298 | Phosphohistidine; by autocatalysisCurated | 1 | |
Modified residuei | 747 | Phosphohistidine; by HPrCurated | 1 |
Keywords - PTMi
PhosphoproteinFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 90 | HPrPROSITE-ProRule annotationAdd BLAST | 90 | |
Domaini | 685 – 828 | PTS EIIA type-2PROSITE-ProRule annotationAdd BLAST | 144 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 119 – 650 | PTS EIBy similarityAdd BLAST | 532 | |
Regioni | 563 – 564 | PEP bindingBy similarity | 2 |
Domaini
The PTS EIIA type-2 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-2 domain.PROSITE-ProRule annotation
In contrast to classical PTS systems, the fructose-like PTS has no requirement for HPr and Enzyme I; FryA combines a IIA domain with an Enzyme I and a HPr domains.Curated
Sequence similaritiesi
Belongs to the PEP-utilizing enzyme family.Curated
Phylogenomic databases
eggNOGi | COG1080, Bacteria COG1762, Bacteria COG1925, Bacteria |
HOGENOMi | CLU_007308_5_1_6 |
Family and domain databases
CDDi | cd00367, PTS-HPr_like, 1 hit cd00211, PTS_IIA_fru, 1 hit |
Gene3Di | 1.10.274.10, 1 hit 3.20.20.60, 1 hit 3.30.1340.10, 1 hit 3.40.930.10, 1 hit |
InterProi | View protein in InterPro IPR000032, HPr-like IPR035895, HPr-like_sf IPR008279, PEP-util_enz_mobile_dom IPR000121, PEP_util_C IPR023151, PEP_util_CS IPR036637, Phosphohistidine_dom_sf IPR016152, PTrfase/Anion_transptr IPR006318, PTS_EI-like IPR002178, PTS_EIIA_type-2_dom IPR008731, PTS_EIN IPR004715, PTS_IIA_fruc IPR036618, PtsI_HPr-bd_sf IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf |
Pfami | View protein in Pfam PF05524, PEP-utilisers_N, 1 hit PF00391, PEP-utilizers, 1 hit PF02896, PEP-utilizers_C, 1 hit PF00381, PTS-HPr, 1 hit PF00359, PTS_EIIA_2, 1 hit |
SUPFAMi | SSF47831, SSF47831, 1 hit SSF51621, SSF51621, 1 hit SSF52009, SSF52009, 1 hit SSF55594, SSF55594, 1 hit SSF55804, SSF55804, 1 hit |
TIGRFAMsi | TIGR00848, fruA, 1 hit TIGR01417, PTS_I_fam, 1 hit |
PROSITEi | View protein in PROSITE PS00742, PEP_ENZYMES_2, 1 hit PS51094, PTS_EIIA_TYPE_2, 1 hit PS51350, PTS_HPR_DOM, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8XBQ8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLTIQFLCPL PNGLHARPAW ELKEQCSQWQ SEITFINHRQ NAKADAKSSL
60 70 80 90 100
ALIGTGTLFN DSCSLNISGS DEEQARRVLE EYIQVRFIDS DSVQPTQAEL
110 120 130 140 150
TAHPLPRSLS RLNPDLLYGN VLASGVGVGT LTLLQSDSLD SYRAIPASAQ
160 170 180 190 200
DSTRLEHSLA TLAEQLNQQL RERDGESKTI LSAHLSLIQD DEFAGNIRRL
210 220 230 240 250
MTEQHQGLGA AIIRNMEQVC AKLSASASDY LRERVSDIRD ISEQLLHITW
260 270 280 290 300
PELKPRNNLV LEKPTILVAE DLTPSQFLSL DLKNLAGMIL EKTGRTSHTL
310 320 330 340 350
ILARASAIPV LSGLPLDAIA RYAGQPAVLD AQCGVLAINP NDAVSGYYQV
360 370 380 390 400
AQTLADKRQK QQAQAAAQLA YSRDNKRIDI AANIGTALEA PGAFANGAEG
410 420 430 440 450
VGLFRTEMLY MDRDSEPDEQ EQFEAYQQVL LAAGDKPIIF RTMDIGGDKS
460 470 480 490 500
IPYLNIPQEE NPFLGYRAVR IYPEFAGLFR TQLRAILRAA SFGNAQLMIP
510 520 530 540 550
MVHGLDQILW VKGEIQKAIV ELKRDGLRHA ETITLGIMVE VPSVCYIIDH
560 570 580 590 600
FCDEVDFFSI GSNDMTQYLY AVDRNNPRVS PLYNPITPSF LRMLQQIVTT
610 620 630 640 650
AHQRGKWVGI CGELGGESRY LPLLLGLGLD ELSMSSPRIP AVKSQLRQLD
660 670 680 690 700
SEACRELARQ ACECRSAQEI EALLTAFTPE EDVRPLLALE NIFVDQAFSN
710 720 730 740 750
KEQAIQFLCG NLGVNGRTEH PFELEEDVWQ REEIVTTGVG FGVAIPHTKS
760 770 780 790 800
QWIRHSSISI ARLAKPVDWQ SEMGEVELVI MLTLGANEGM NHVKVFSQLA
810 820 830
RKLVNKNFRQ SLFAAQDAQS ILTLLETELT F
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005174 Genomic DNA Translation: AAG57509.1 BA000007 Genomic DNA Translation: BAB36686.1 |
PIRi | A85881 G91036 |
RefSeqi | NP_311290.1, NC_002695.1 WP_000955897.1, NZ_SWKA01000005.1 |
Genome annotation databases
EnsemblBacteriai | AAG57509; AAG57509; Z3648 BAB36686; BAB36686; ECs_3263 |
GeneIDi | 915635 |
KEGGi | ece:Z3648 ecs:ECs3263 |
PATRICi | fig|386585.9.peg.3407 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005174 Genomic DNA Translation: AAG57509.1 BA000007 Genomic DNA Translation: BAB36686.1 |
PIRi | A85881 G91036 |
RefSeqi | NP_311290.1, NC_002695.1 WP_000955897.1, NZ_SWKA01000005.1 |
3D structure databases
SMRi | Q8XBQ8 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 155864.EDL933_3552 |
Genome annotation databases
EnsemblBacteriai | AAG57509; AAG57509; Z3648 BAB36686; BAB36686; ECs_3263 |
GeneIDi | 915635 |
KEGGi | ece:Z3648 ecs:ECs3263 |
PATRICi | fig|386585.9.peg.3407 |
Phylogenomic databases
eggNOGi | COG1080, Bacteria COG1762, Bacteria COG1925, Bacteria |
HOGENOMi | CLU_007308_5_1_6 |
Family and domain databases
CDDi | cd00367, PTS-HPr_like, 1 hit cd00211, PTS_IIA_fru, 1 hit |
Gene3Di | 1.10.274.10, 1 hit 3.20.20.60, 1 hit 3.30.1340.10, 1 hit 3.40.930.10, 1 hit |
InterProi | View protein in InterPro IPR000032, HPr-like IPR035895, HPr-like_sf IPR008279, PEP-util_enz_mobile_dom IPR000121, PEP_util_C IPR023151, PEP_util_CS IPR036637, Phosphohistidine_dom_sf IPR016152, PTrfase/Anion_transptr IPR006318, PTS_EI-like IPR002178, PTS_EIIA_type-2_dom IPR008731, PTS_EIN IPR004715, PTS_IIA_fruc IPR036618, PtsI_HPr-bd_sf IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf |
Pfami | View protein in Pfam PF05524, PEP-utilisers_N, 1 hit PF00391, PEP-utilizers, 1 hit PF02896, PEP-utilizers_C, 1 hit PF00381, PTS-HPr, 1 hit PF00359, PTS_EIIA_2, 1 hit |
SUPFAMi | SSF47831, SSF47831, 1 hit SSF51621, SSF51621, 1 hit SSF52009, SSF52009, 1 hit SSF55594, SSF55594, 1 hit SSF55804, SSF55804, 1 hit |
TIGRFAMsi | TIGR00848, fruA, 1 hit TIGR01417, PTS_I_fam, 1 hit |
PROSITEi | View protein in PROSITE PS00742, PEP_ENZYMES_2, 1 hit PS51094, PTS_EIIA_TYPE_2, 1 hit PS51350, PTS_HPR_DOM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PTFX_ECO57 | |
Accessioni | Q8XBQ8Primary (citable) accession number: Q8XBQ8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 15, 2003 |
Last sequence update: | March 1, 2002 | |
Last modified: | April 7, 2021 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families