UniProtKB - Q8XBJ2 (MURQ_ECO57)
Protein
N-acetylmuramic acid 6-phosphate etherase
Gene
murQ
Organism
Escherichia coli O157:H7
Status
Functioni
Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D-lactate. Together with AnmK, is also required for the utilization of anhydro-N-acetylmuramic acid (anhMurNAc) either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling.UniRule annotation
Miscellaneous
A lyase-type mechanism (elimination/hydration) is suggested for the cleavage of the lactyl ether bond of MurNAc 6-phosphate, with the formation of an alpha,beta-unsaturated aldehyde intermediate with (E)-stereochemistry, followed by the syn addition of water to give product.UniRule annotation
Catalytic activityi
- H2O + N-acetyl-D-muramate 6-phosphate = (R)-lactate + N-acetyl-D-glucosamine 6-phosphateUniRule annotationEC:4.2.1.126UniRule annotation
: 1,6-anhydro-N-acetylmuramate degradation Pathwayi
This protein is involved in the pathway 1,6-anhydro-N-acetylmuramate degradation, which is part of Amino-sugar metabolism.UniRule annotationView all proteins of this organism that are known to be involved in the pathway 1,6-anhydro-N-acetylmuramate degradation and in Amino-sugar metabolism.
Pathwayi: N-acetylmuramate degradation
This protein is involved in the pathway N-acetylmuramate degradation, which is part of Amino-sugar metabolism.UniRule annotationView all proteins of this organism that are known to be involved in the pathway N-acetylmuramate degradation and in Amino-sugar metabolism.
Pathwayi: peptidoglycan recycling
This protein is involved in the pathway peptidoglycan recycling, which is part of Cell wall biogenesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway peptidoglycan recycling and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 83 | Proton donorUniRule annotation | 1 | |
Active sitei | 114 | UniRule annotation | 1 |
GO - Molecular functioni
- carbohydrate derivative binding Source: InterPro
- carbon-oxygen lyase activity Source: UniProtKB-UniRule
GO - Biological processi
- 1,6-anhydro-N-acetyl-beta-muramic acid catabolic process Source: UniProtKB-UniPathway
- carbohydrate metabolic process Source: UniProtKB-KW
- N-acetylmuramic acid catabolic process Source: UniProtKB-UniPathway
- peptidoglycan turnover Source: UniProtKB-UniRule
Keywordsi
Molecular function | Lyase |
Biological process | Carbohydrate metabolism |
Enzyme and pathway databases
BioCyci | ECOO157:YFEU-MONOMER |
UniPathwayi | UPA00342 UPA00343 UPA00544 |
Names & Taxonomyi
Protein namesi | Recommended name: N-acetylmuramic acid 6-phosphate etheraseUniRule annotation (EC:4.2.1.126UniRule annotation)Short name: MurNAc-6-P etheraseUniRule annotation Alternative name(s): N-acetylmuramic acid 6-phosphate hydrolaseUniRule annotation N-acetylmuramic acid 6-phosphate lyaseUniRule annotation |
Gene namesi | Name:murQUniRule annotation Synonyms:yfeU Ordered Locus Names:Z3693, ECs3299 |
Organismi | Escherichia coli O157:H7 |
Taxonomic identifieri | 83334 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000214830 | 1 – 298 | N-acetylmuramic acid 6-phosphate etheraseAdd BLAST | 298 |
Expressioni
Inductioni
Induced by MurNAc 6-phosphate that releases the repressor MurR from the DNA. Repressed by MurR in the absence of MurNAc 6-phosphate.UniRule annotation
Interactioni
Subunit structurei
Homodimer.
UniRule annotationProtein-protein interaction databases
STRINGi | 155864.EDL933_3591 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 55 – 218 | SISUniRule annotationAdd BLAST | 164 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG4105E15 Bacteria COG2103 LUCA |
HOGENOMi | HOG000084045 |
KOi | K07106 |
Family and domain databases
CDDi | cd05007 SIS_Etherase, 1 hit |
HAMAPi | MF_00068 MurQ, 1 hit |
InterProi | View protein in InterPro IPR005488 Etherase_MurQ IPR005486 Glucokinase_regulatory_CS IPR040190 MURQ/GCKR IPR001347 SIS |
PANTHERi | PTHR10088 PTHR10088, 1 hit PTHR10088:SF5 PTHR10088:SF5, 1 hit |
Pfami | View protein in Pfam PF01380 SIS, 1 hit |
TIGRFAMsi | TIGR00274 TIGR00274, 1 hit |
PROSITEi | View protein in PROSITE PS01272 GCKR, 1 hit PS51464 SIS, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8XBJ2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQLEKMITEG SNAASAEIDR VSTLEMCRII NDEDKTVPLA VERVLPDIAA
60 70 80 90 100
AIDVIHAQVS GGGRLIYLGA GTSGRLGILD ASECPPTYGV KPGLVVGLIA
110 120 130 140 150
GGEYAIQHAV EGAEDSREGG VNDLKNIGLT AQDVVVGIAA SGRTPYVIAG
160 170 180 190 200
LEYARQLGCR TVGISCNPGS AVSTTAEFAI TPVVGAEVVT GSSRMKAGTA
210 220 230 240 250
QKLVLNMLST GLMIKSGKVF GNLMVDVVAT NEKLHVRQVN IVKNATGCNA
260 270 280 290
EQAEAALIAC ERNCKTAIVM VLKNLDAAEA KKRLDQHGGF IRQVLDKE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005174 Genomic DNA Translation: AAG57546.1 BA000007 Genomic DNA Translation: BAB36722.1 |
PIRi | C91041 F85885 |
RefSeqi | NP_311326.1, NC_002695.1 WP_001175615.1, NZ_SDVX01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAG57546; AAG57546; Z3693 BAB36722; BAB36722; BAB36722 |
GeneIDi | 915479 |
KEGGi | ece:Z3693 ecs:ECs3299 |
PATRICi | fig|386585.9.peg.3446 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005174 Genomic DNA Translation: AAG57546.1 BA000007 Genomic DNA Translation: BAB36722.1 |
PIRi | C91041 F85885 |
RefSeqi | NP_311326.1, NC_002695.1 WP_001175615.1, NZ_SDVX01000004.1 |
3D structure databases
SMRi | Q8XBJ2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 155864.EDL933_3591 |
Genome annotation databases
EnsemblBacteriai | AAG57546; AAG57546; Z3693 BAB36722; BAB36722; BAB36722 |
GeneIDi | 915479 |
KEGGi | ece:Z3693 ecs:ECs3299 |
PATRICi | fig|386585.9.peg.3446 |
Phylogenomic databases
eggNOGi | ENOG4105E15 Bacteria COG2103 LUCA |
HOGENOMi | HOG000084045 |
KOi | K07106 |
Enzyme and pathway databases
UniPathwayi | UPA00342 UPA00343 UPA00544 |
BioCyci | ECOO157:YFEU-MONOMER |
Family and domain databases
CDDi | cd05007 SIS_Etherase, 1 hit |
HAMAPi | MF_00068 MurQ, 1 hit |
InterProi | View protein in InterPro IPR005488 Etherase_MurQ IPR005486 Glucokinase_regulatory_CS IPR040190 MURQ/GCKR IPR001347 SIS |
PANTHERi | PTHR10088 PTHR10088, 1 hit PTHR10088:SF5 PTHR10088:SF5, 1 hit |
Pfami | View protein in Pfam PF01380 SIS, 1 hit |
TIGRFAMsi | TIGR00274 TIGR00274, 1 hit |
PROSITEi | View protein in PROSITE PS01272 GCKR, 1 hit PS51464 SIS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MURQ_ECO57 | |
Accessioni | Q8XBJ2Primary (citable) accession number: Q8XBJ2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 22, 2003 |
Last sequence update: | March 1, 2002 | |
Last modified: | May 8, 2019 | |
This is version 107 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - PATHWAY comments
Index of metabolic and biosynthesis pathways