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Protein

Probable diguanylate cyclase DgcF

Gene

dgcF

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the synthesis of cyclic-di-GMP (c-di-GMP) via the condensation of 2 GTP molecules.By similarity

Catalytic activityi

2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Pathwayi: 3',5'-cyclic di-GMP biosynthesis

This protein is involved in the pathway 3',5'-cyclic di-GMP biosynthesis, which is part of Purine metabolism.Curated
View all proteins of this organism that are known to be involved in the pathway 3',5'-cyclic di-GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi181MagnesiumBy similarity1
Metal bindingi182Magnesium; via carbonyl oxygenBy similarity1
Binding sitei189SubstrateBy similarity1
Binding sitei194Substrate; via carbonyl oxygenBy similarity1
Binding sitei198SubstrateBy similarity1
Metal bindingi224MagnesiumBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00599

Names & Taxonomyi

Protein namesi
Recommended name:
Probable diguanylate cyclase DgcFCurated (EC:2.7.7.65By similarity)
Short name:
DGCCurated
Gene namesi
Name:dgcFBy similarity
Synonyms:yneF
Ordered Locus Names:Z2182, ECs2129
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei10 – 30HelicalSequence analysisAdd BLAST21
Transmembranei41 – 61HelicalSequence analysisAdd BLAST21
Transmembranei80 – 100HelicalSequence analysisAdd BLAST21
Transmembranei116 – 136HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002013371 – 315Probable diguanylate cyclase DgcFAdd BLAST315

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi155864.Z2182

Structurei

3D structure databases

ProteinModelPortaliQ8XAZ4
SMRiQ8XAZ4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini173 – 310GGDEFPROSITE-ProRule annotationAdd BLAST138

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU Bacteria
ENOG410XNMH LUCA
HOGENOMiHOG000120740
OMAiATWLAWF

Family and domain databases

CDDicd01949 GGDEF, 1 hit
InterProiView protein in InterPro
IPR000160 GGDEF_dom
IPR029787 Nucleotide_cyclase
PfamiView protein in Pfam
PF00990 GGDEF, 1 hit
SMARTiView protein in SMART
SM00267 GGDEF, 1 hit
SUPFAMiSSF55073 SSF55073, 1 hit
TIGRFAMsiTIGR00254 GGDEF, 1 hit
PROSITEiView protein in PROSITE
PS50887 GGDEF, 1 hit

Sequencei

Sequence statusi: Complete.

Q8XAZ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLADWFSEQF STGVLIVPCM LTLAIPGVLP RFKAEQMMPA IALIVSVIAS
60 70 80 90 100
VVIGGAGSLA FPLPALIWCA VRYTPQVTCL LTFVTGAVEI VLVANSVIDI
110 120 130 140 150
SVGSPFSIPQ MFSARLGIAT MAICPIMVSF SVAAINSLMK QVALRADFDF
160 170 180 190 200
LTQVYSRSGL YEALKSPSLK QTQHLTVMLL DIDYFKSIND NYGHECGDKV
210 220 230 240 250
LSVFARHIQK IVGDKGLVAR MGGEEFAVAV PSVNPVDGLL MAEKIRKGVE
260 270 280 290 300
LQPFTWQQKT LYLTVSIGVG SGRASYRTLT DDFNKLMVEA DTCLYRSKKD
310
GRNRTSTMRY GEEVV
Length:315
Mass (Da):34,365
Last modified:March 27, 2002 - v1
Checksum:i9342AB25E5EA45A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG56244.1
BA000007 Genomic DNA Translation: BAB35552.1
PIRiA90895
H85722
RefSeqiNP_310156.1, NC_002695.1
WP_000878970.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG56244; AAG56244; Z2182
BAB35552; BAB35552; BAB35552
GeneIDi917328
KEGGiece:Z2182
ecs:ECs2129
PATRICifig|386585.9.peg.2235

Similar proteinsi

Entry informationi

Entry nameiDGCF_ECO57
AccessioniPrimary (citable) accession number: Q8XAZ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: April 25, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health