Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Urease subunit alpha

Gene

ureC1

more
Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Caution

Neither O157 strain expresses urease due to a truncation of ureD, the last gene of the probable operon. Urease activity is restored in O157 / Sakai upon complementation with wild-type ureD.Curated
This region of the chromosome is duplicated in strain O157:H7 / EDL933 but not in O157:H7 / Sakai.Curated

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit beta (ureB1), Urease subunit gamma (ureA1), Urease subunit alpha (ureC1)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi135Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi137Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi218Nickel 1; via carbamate groupUniRule annotation1
Metal bindingi218Nickel 2; via carbamate groupUniRule annotation1
Binding sitei220SubstrateUniRule annotation1
Metal bindingi247Nickel 2; via pros nitrogenUniRule annotation1
Metal bindingi273Nickel 2; via tele nitrogenUniRule annotation1
Active sitei321Proton donorUniRule annotation1
Metal bindingi361Nickel 1UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370

Protein family/group databases

MEROPSiM38.982

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureC1UniRule annotation
Ordered Locus Names:Z1145, ECs1324
AND
Name:ureC2UniRule annotation
Ordered Locus Names:Z1584
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002341551 – 568Urease subunit alphaAdd BLAST568

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei218N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Proteomic databases

PRIDEiQ8XAG0

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi155864.Z1584

Structurei

3D structure databases

ProteinModelPortaliQ8XAG0
SMRiQ8XAG0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini130 – 568UreaseUniRule annotationAdd BLAST439

Sequence similaritiesi

Belongs to the metallo-dependent hydrolases superfamily. Urease alpha subunit family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM Bacteria
COG0804 LUCA
HOGENOMiHOG000075064
KOiK01428
OMAiGFDSHIH

Family and domain databases

CDDicd00375 Urease_alpha, 1 hit
Gene3Di2.30.40.10, 2 hits
HAMAPiMF_01953 Urease_alpha, 1 hit
InterProiView protein in InterPro
IPR006680 Amidohydro-rel
IPR011059 Metal-dep_hydrolase_composite
IPR032466 Metal_Hydrolase
IPR011612 Urease_alpha_N_dom
IPR017950 Urease_AS
IPR005848 Urease_asu
IPR017951 Urease_asu_c
IPR029754 Urease_Ni-bd
PANTHERiPTHR43440 PTHR43440, 1 hit
PfamiView protein in Pfam
PF01979 Amidohydro_1, 1 hit
PF00449 Urease_alpha, 1 hit
PRINTSiPR01752 UREASE
SUPFAMiSSF51338 SSF51338, 2 hits
SSF51556 SSF51556, 2 hits
TIGRFAMsiTIGR01792 urease_alph, 1 hit
PROSITEiView protein in PROSITE
PS01120 UREASE_1, 1 hit
PS00145 UREASE_2, 1 hit
PS51368 UREASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q8XAG0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSNISRQAY ADMFGPTTGD KIRLADTELW IEVEDDLTTY GEEVKFGGGK
60 70 80 90 100
VIRDGMGQGQ MLSAGCADLV LTNALIIDYW GIVKADIGVK DGRIFAIGKA
110 120 130 140 150
GNPDIQPNVT IPIGVSTEII AAEGRIVTAG GVDTHIHWIC PQQAEEALTS
160 170 180 190 200
GITTMIGGGT GPTAGSNATT CTPGPWYIYQ MLQAADSLPV NIGLLGKGNC
210 220 230 240 250
SNPDALREQV AAGVIGLKIH EDWGATPAVI NCALTVADEM DVQVALHSDT
260 270 280 290 300
LNESGFVEDT LTAIGGRTIH TFHTEGAGGG HAPDIITACA HPNILPSSTN
310 320 330 340 350
PTLPYTVNTI DEHLDMLMVC HHLDPDIAED VAFAESRIRQ ETIAAEDVLH
360 370 380 390 400
DLGAFSLTSS DSQAMGRVGE VVLRTWQVAH RMKVQRGPLP EESGDNDNVR
410 420 430 440 450
VKRYIAKYTI NPALTHGIAH EVGSIEVGKL ADLVLWSPAF FGVKPATIVK
460 470 480 490 500
GGMIAMAPMG DINGSIPTPQ PVHYRPMFAA LGSARHRCRV TFLSQAAAAN
510 520 530 540 550
GVAEQLNLHS TTAVVKGCRT VQKADMRHNS LLPDITVDSQ TYEVRINGEL
560
ITSEPADILP MAQRYFLF
Length:568
Mass (Da):60,626
Last modified:March 1, 2002 - v1
Checksum:iAC39B7B701481361
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG55290.1
AE005174 Genomic DNA Translation: AAG55699.1
BA000007 Genomic DNA Translation: BAB34747.1
PIRiD90794
G85654
RefSeqiNP_309351.1, NC_002695.1
WP_001301487.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG55290; AAG55290; Z1145
AAG55699; AAG55699; Z1584
BAB34747; BAB34747; BAB34747
GeneIDi913507
KEGGiece:Z1145
ece:Z1584
ecs:ECs1324
PATRICifig|386585.9.peg.1429

Similar proteinsi

Entry informationi

Entry nameiURE1_ECO57
AccessioniPrimary (citable) accession number: Q8XAG0
Secondary accession number(s): Q7AFH5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: March 1, 2002
Last modified: April 25, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health