UniProtKB - Q8X5H9 (FTSK_ECO57)
Protein
DNA translocase FtsK
Gene
ftsK
Organism
Escherichia coli O157:H7
Status
Functioni
Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Translocation stops specifically at Xer-dif sites, where FtsK interacts with the Xer recombinase, allowing activation of chromosome unlinking by recombination. FtsK orienting polar sequences (KOPS) guide the direction of DNA translocation. FtsK can remove proteins from DNA as it translocates, but translocation stops specifically at XerCD-dif site, thereby preventing removal of XerC and XerD from dif (By similarity).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 1007 – 1012 | ATPPROSITE-ProRule annotation | 6 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA binding Source: UniProtKB-KW
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- chromosome segregation Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding |
Biological process | Cell cycle, Cell division, Chromosome partition |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ECOO157:FTSK-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: DNA translocase FtsK |
Gene namesi | Name:ftsK Ordered Locus Names:Z1235, ECs0975 |
Organismi | Escherichia coli O157:H7 |
Taxonomic identifieri | 83334 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane By similarity; Multi-pass membrane protein By similarity Note: Located at the septum.By similarity
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 24 | CytoplasmicBy similarityAdd BLAST | 24 | |
Transmembranei | 25 – 44 | HelicalBy similarityAdd BLAST | 20 | |
Topological domaini | 45 – 74 | PeriplasmicBy similarityAdd BLAST | 30 | |
Transmembranei | 75 – 98 | HelicalBy similarityAdd BLAST | 24 | |
Topological domaini | 99 – 115 | CytoplasmicBy similarityAdd BLAST | 17 | |
Transmembranei | 116 – 132 | HelicalBy similarityAdd BLAST | 17 | |
Topological domaini | 133 – 162 | PeriplasmicBy similarityAdd BLAST | 30 | |
Transmembranei | 163 – 179 | HelicalBy similarityAdd BLAST | 17 | |
Topological domaini | 180 – 1342 | CytoplasmicBy similarityAdd BLAST | 1163 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000098258 | 1 – 1342 | DNA translocase FtsKAdd BLAST | 1342 |
Proteomic databases
PRIDEi | Q8X5H9 |
Interactioni
Subunit structurei
Homohexamer.
Forms a ring that surrounds DNA (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 155864.EDL933_1154 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 987 – 1200 | FtsKPROSITE-ProRule annotationAdd BLAST | 214 |
Domaini
Consists of an N-terminal domain, which is sufficient for the localization to the septal ring and is required for cell division, followed by a linker domain, and a C-terminal domain, which forms the translocation motor involved in chromosome segregation. The C-terminal domain can be further subdivided into alpha, beta and gamma subdomains. The alpha and beta subdomains multimerise to produce a hexameric ring, contain the nucleotide binding motif and form the DNA pump. The gamma subdomain is a regulatory subdomain that controls translocation of DNA by recognition of KOPS motifs and interacts with XerD recombinase (By similarity).By similarity
Sequence similaritiesi
Belongs to the FtsK/SpoIIIE/SftA family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG4105CNU Bacteria COG1674 LUCA |
HOGENOMi | HOG000010001 |
KOi | K03466 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR025199 FtsK_4TM IPR041027 FtsK_alpha IPR002543 FtsK_dom IPR018541 Ftsk_gamma IPR027417 P-loop_NTPase IPR036388 WH-like_DNA-bd_sf IPR036390 WH_DNA-bd_sf |
Pfami | View protein in Pfam PF13491 FtsK_4TM, 1 hit PF17854 FtsK_alpha, 1 hit PF09397 FtsK_gamma, 1 hit PF01580 FtsK_SpoIIIE, 1 hit |
SMARTi | View protein in SMART SM00843 Ftsk_gamma, 1 hit |
SUPFAMi | SSF46785 SSF46785, 1 hit SSF52540 SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50901 FTSK, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8X5H9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQEYTEDKE VTLTKLSSGR RLLEALLILI VLFAVWLMAA LLSFNPSDPS
60 70 80 90 100
WSQTAWHEPI HNLGGMPGAW LADTLFFIFG VMAYTIPVII VGGCWFAWRH
110 120 130 140 150
QSSDENIDYF AVSLRIIGVL ALILTSCGLA AINADDIWYF ASGGVIGSLL
160 170 180 190 200
STTLQPLLHS SGGTIALLCV WAAGLTLFTG WSWVTIAEKL GGWILNILTF
210 220 230 240 250
ASNRTRRDDT WVDEDEYEDD EEYEDENHGK QHESRRARIL RGALARRKRL
260 270 280 290 300
AEKFINPMGR QTDAALFSGK RMDDDEEITY TARGVAADPD DVLFSGNRAT
310 320 330 340 350
QPEYDEYDPL LNGAPITEPV AVAAAATTAT QSWAAPVEPV TQTPPVASVD
360 370 380 390 400
VPPSQPTVAW QPVPGPQTGE PVIAPAPEGY PQQSQYAQPA VQYNEPLQQP
410 420 430 440 450
VQPQQPYYAP AAEQPAQQPY YAPAPEQPVA GNAWQAEEQQ STFAPQSTYQ
460 470 480 490 500
TEQTYQQPAA QEPLYQQPQP VEQQPVVEPE PVVEETKPAR PPLYYFEEVE
510 520 530 540 550
EKRAREREQL AAWYQPIPEP VKEPEPIKSS LKAPSVAAVP PVEAAAAVSP
560 570 580 590 600
LASGVKKATL ATGAAATVAA PVFSLANSGG PRPQVKEGIG PQLPRPKRIR
610 620 630 640 650
VPTRRELASY GIKLPSQRAA EEKAREAQRN QYDSGDQYND DEIDAMQQDE
660 670 680 690 700
LARQFAQTQQ QRYGEQYQHD VPVNAEDADA AAEAELARQF AQTQQQRYSG
710 720 730 740 750
EQPAGANPFS LDDFEFSPMK ALLDDGPHEP LFTPIVEPVQ QPQQPVAPQQ
760 770 780 790 800
QYQQPQQPVA PQPQYQQPQQ QVAPQPQYQQ PQQPVAPQQQ YQQPQQPVAP
810 820 830 840 850
QPQYQQPQQP VAPQPQYQQP QQPVAPQPQD TLLHPLLMRN GDSRPLHKPT
860 870 880 890 900
TPLPSLDLLT PPPSEVEPVD TFALEQMARL VEARLADFRI KADVVNYSPG
910 920 930 940 950
PVITRFELNL APGVKAARIS NLSRDLARSL STVAVRVVEV IPGKPYVGLE
960 970 980 990 1000
LPNKKRQTVY LREVLDNAKF RDNPSPLTVV LGKDIAGEPV VADLAKMPHL
1010 1020 1030 1040 1050
LVAGTTGSGK SVGVNAMILS MLYKAQPEDV RFIMIDPKML ELSVYEGIPH
1060 1070 1080 1090 1100
LLTEVVTDMK DAANALRWCV NEMERRYKLM SALGVRNLAG YNEKIAEADR
1110 1120 1130 1140 1150
MMRPIPDPYW KPGDSMDAQH PVLKKEPYIV VLVDEFADLM MTVGKKVEEL
1160 1170 1180 1190 1200
IARLAQKARA AGIHLVLATQ RPSVDVITGL IKANIPTRIA FTVSSKIDSR
1210 1220 1230 1240 1250
TILDQAGAES LLGMGDMLYS GPNSTLPVRV HGAFVRDQEV HAVVQDWKAR
1260 1270 1280 1290 1300
GRPQYVDGIT SDSESEGGAG GFDGAEELDP LFDQAVQFVT EKRKASISGV
1310 1320 1330 1340
QRQFRIGYNR AARIIEQMEA QGIVSEQGHN GNREVLSPPP FD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005174 Genomic DNA Translation: AAG55377.1 BA000007 Genomic DNA Translation: BAB34398.1 |
PIRi | E85614 G90750 |
RefSeqi | NP_309002.1, NC_002695.1 WP_000076999.1, NZ_SDVX01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAG55377; AAG55377; Z1235 BAB34398; BAB34398; BAB34398 |
GeneIDi | 917727 |
KEGGi | ece:Z1235 ecs:ECs0975 |
PATRICi | fig|386585.9.peg.1092 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005174 Genomic DNA Translation: AAG55377.1 BA000007 Genomic DNA Translation: BAB34398.1 |
PIRi | E85614 G90750 |
RefSeqi | NP_309002.1, NC_002695.1 WP_000076999.1, NZ_SDVX01000001.1 |
3D structure databases
SMRi | Q8X5H9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 155864.EDL933_1154 |
Proteomic databases
PRIDEi | Q8X5H9 |
Genome annotation databases
EnsemblBacteriai | AAG55377; AAG55377; Z1235 BAB34398; BAB34398; BAB34398 |
GeneIDi | 917727 |
KEGGi | ece:Z1235 ecs:ECs0975 |
PATRICi | fig|386585.9.peg.1092 |
Phylogenomic databases
eggNOGi | ENOG4105CNU Bacteria COG1674 LUCA |
HOGENOMi | HOG000010001 |
KOi | K03466 |
Enzyme and pathway databases
BioCyci | ECOO157:FTSK-MONOMER |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR025199 FtsK_4TM IPR041027 FtsK_alpha IPR002543 FtsK_dom IPR018541 Ftsk_gamma IPR027417 P-loop_NTPase IPR036388 WH-like_DNA-bd_sf IPR036390 WH_DNA-bd_sf |
Pfami | View protein in Pfam PF13491 FtsK_4TM, 1 hit PF17854 FtsK_alpha, 1 hit PF09397 FtsK_gamma, 1 hit PF01580 FtsK_SpoIIIE, 1 hit |
SMARTi | View protein in SMART SM00843 Ftsk_gamma, 1 hit |
SUPFAMi | SSF46785 SSF46785, 1 hit SSF52540 SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50901 FTSK, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FTSK_ECO57 | |
Accessioni | Q8X5H9Primary (citable) accession number: Q8X5H9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 29, 2003 |
Last sequence update: | March 1, 2002 | |
Last modified: | May 8, 2019 | |
This is version 104 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families