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UniProtKB - Q8X1T3 (PYC_PICAN)
Protein
Pyruvate carboxylase
Gene
PYC
Organism
Pichia angusta (Yeast) (Hansenula polymorpha)
Status
Functioni
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
By similarityCatalytic activityi
- EC:6.4.1.1
Cofactori
Protein has several cofactor binding sites:: gluconeogenesis Pathwayi
This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 140 | ATPBy similarity | 1 | |
Binding sitei | 224 | ATPBy similarity | 1 | |
Binding sitei | 259 | ATPBy similarity | 1 | |
Active sitei | 316 | By similarity | 1 | |
Metal bindingi | 570 | Divalent metal cationBy similarity | 1 | |
Binding sitei | 642 | SubstrateBy similarity | 1 | |
Metal bindingi | 738 | Divalent metal cation; via carbamate groupBy similarity | 1 | |
Metal bindingi | 768 | Divalent metal cationBy similarity | 1 | |
Metal bindingi | 770 | Divalent metal cationBy similarity | 1 | |
Binding sitei | 902 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- pyruvate carboxylase activity Source: UniProtKB-EC
GO - Biological processi
- gluconeogenesis Source: UniProtKB-UniPathway
- pyruvate metabolic process Source: InterPro
Keywordsi
Molecular function | Ligase, Multifunctional enzyme |
Biological process | Gluconeogenesis |
Ligand | ATP-binding, Biotin, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00138 |
Protein family/group databases
MoonProti | Q8X1T3 |
Names & Taxonomyi
Protein namesi | Recommended name: Pyruvate carboxylase (EC:6.4.1.1)Alternative name(s): Pyruvic carboxylase Short name: PCB |
Gene namesi | Name:PYC |
Organismi | Pichia angusta (Yeast) (Hansenula polymorpha) |
Taxonomic identifieri | 870730 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Pichiaceae › Ogataea |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000146821 | 1 – 1175 | Pyruvate carboxylaseAdd BLAST | 1175 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 738 | N6-carboxylysineBy similarity | 1 | |
Modified residuei | 1140 | N6-biotinyllysinePROSITE-ProRule annotationBy similarity | 1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 474 | Biotin carboxylationAdd BLAST | 453 | |
Domaini | 144 – 341 | ATP-graspPROSITE-ProRule annotationAdd BLAST | 198 | |
Domaini | 561 – 828 | Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST | 268 | |
Domaini | 1099 – 1174 | Biotinyl-bindingPROSITE-ProRule annotationAdd BLAST | 76 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 569 – 573 | Substrate bindingBy similarity | 5 |
Phylogenomic databases
PhylomeDBi | Q8X1T3 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR011761, ATP-grasp IPR005481, BC-like_N IPR001882, Biotin_BS IPR011764, Biotin_carboxylation_dom IPR005482, Biotin_COase_C IPR000089, Biotin_lipoyl IPR003379, Carboxylase_cons_dom IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR016185, PreATP-grasp_dom_sf IPR000891, PYR_CT IPR005930, Pyruv_COase IPR011054, Rudment_hybrid_motif IPR011053, Single_hybrid_motif |
Pfami | View protein in Pfam PF02785, Biotin_carb_C, 1 hit PF00289, Biotin_carb_N, 1 hit PF00364, Biotin_lipoyl, 1 hit PF02786, CPSase_L_D2, 1 hit PF00682, HMGL-like, 1 hit PF02436, PYC_OADA, 1 hit |
PIRSFi | PIRSF001594, Pyruv_carbox, 1 hit |
SMARTi | View protein in SMART SM00878, Biotin_carb_C, 1 hit |
SUPFAMi | SSF51230, SSF51230, 1 hit SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01235, pyruv_carbox, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS50979, BC, 1 hit PS00188, BIOTIN, 1 hit PS50968, BIOTINYL_LIPOYL, 1 hit PS00866, CPSASE_1, 1 hit PS00867, CPSASE_2, 1 hit PS50991, PYR_CT, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8X1T3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQVEDYSSL HRLRKNSEIL SNANKILVAN RGEIPIRIFR SAHELSMQTV
60 70 80 90 100
AIYSHEDRLS MHRLKADEAY VIGARGQYSP VQAYLQIDEI INIALEHNVS
110 120 130 140 150
MIHPGYGFLS ENSEFARKVE DSGMIWIGPP HNVIDAVGDK VSARNLAGKC
160 170 180 190 200
NVPVVPGTDG PIDSVEQAQE FVDKYGYPVI IKAAFGGGGR GMRVVREGES
210 220 230 240 250
IADAFQRATS EAKTAFGNGT CFIERFLDKP KHIEVQLLAD NYGNVIHLFE
260 270 280 290 300
RDCSVQRRHQ KVVEIAPAKT LPVEVRDAIL TDAVKLAKAA NYRNAGTAEF
310 320 330 340 350
LVDNQNRHYF IEINPRIQVE HTITEEVTGV DIVAAQIQIA AGASLQQLGL
360 370 380 390 400
LQDKITTRGF AIQCRITTED PAKNFQPDTG KIEVYRSSGG NGVRLDGGNG
410 420 430 440 450
FAGAIISPHY DSMLVKCSTS GSNYEIARRK MIRALVEFRI RGVKTNIPFL
460 470 480 490 500
LALLTHPTFV SGDCWTTFID DTPSLFEMVQ SKNRAQKLLS YLADLCVNGS
510 520 530 540 550
SIKGQIGLPK LTRDADIPVI HDINGWDIDI KNTPPPESFR QYLLDYGPEQ
560 570 580 590 600
FANQIRAFDG CLIMDTTWRD AHQSLLATRV RTIDLLNIAP ATAHAFRYAF
610 620 630 640 650
ALECWGGATF DVAMRFLHED PWDRLRKLRK AVPNIPFQML LRGANGVAYS
660 670 680 690 700
SLPDNAIDHF VKQAKDAGVD IFRVFDALND LEQLKVGVDA VKKAGGVVEA
710 720 730 740 750
TVCYSGDMLK PGKKYNLKYY LETVDKIMEM GTHLLGIKDM AGTLKPAAAK
760 770 780 790 800
LLISSIRKKY PSVPIHVHTH DSAGTGVITY VACALAGADV VDCAVNSMSG
810 820 830 840 850
LTSQPSMSAF IAALDNEINT GITEQNAREI DAYWSEMRLL YSCFEADLKG
860 870 880 890 900
PDPEVYNHEI PGGQLTNLLF QAQQVGLGEK WLETKKAYEE ANMLLGDIVK
910 920 930 940 950
VTPTSKVVGD LAQFMVSNKL SPKDVERLAS ELDFPDSVLD FFEGLMGTPY
960 970 980 990 1000
GGFPEPLRTN ILAGKRRKLT RRPGLELEPF DLKKIKEELQ SRFGNSITEC
1010 1020 1030 1040 1050
DVASYNMYPK VFESFKKIQE KYGDLSVLPT RFFLAPPKLN EEISVEIEQG
1060 1070 1080 1090 1100
KTFVIKVMAI GDLSPQTGTR EVYFEFNGEM RKVTVEDKLA AVETVTRPKA
1110 1120 1130 1140 1150
DAHNPNEVGA PMAGVVIEVR VHPGVEVKKG DPLCVLSAMK MEMVISSPVS
1160 1170
GRVGEVIVHE NDSVDAGDLI CKITK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF221670 Genomic DNA Translation: AAL69566.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF221670 Genomic DNA Translation: AAL69566.1 |
3D structure databases
AlphaFoldDBi | Q8X1T3 |
SMRi | Q8X1T3 |
ModBasei | Search... |
Protein family/group databases
MoonProti | Q8X1T3 |
Phylogenomic databases
PhylomeDBi | Q8X1T3 |
Enzyme and pathway databases
UniPathwayi | UPA00138 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR011761, ATP-grasp IPR005481, BC-like_N IPR001882, Biotin_BS IPR011764, Biotin_carboxylation_dom IPR005482, Biotin_COase_C IPR000089, Biotin_lipoyl IPR003379, Carboxylase_cons_dom IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR016185, PreATP-grasp_dom_sf IPR000891, PYR_CT IPR005930, Pyruv_COase IPR011054, Rudment_hybrid_motif IPR011053, Single_hybrid_motif |
Pfami | View protein in Pfam PF02785, Biotin_carb_C, 1 hit PF00289, Biotin_carb_N, 1 hit PF00364, Biotin_lipoyl, 1 hit PF02786, CPSase_L_D2, 1 hit PF00682, HMGL-like, 1 hit PF02436, PYC_OADA, 1 hit |
PIRSFi | PIRSF001594, Pyruv_carbox, 1 hit |
SMARTi | View protein in SMART SM00878, Biotin_carb_C, 1 hit |
SUPFAMi | SSF51230, SSF51230, 1 hit SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01235, pyruv_carbox, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS50979, BC, 1 hit PS00188, BIOTIN, 1 hit PS50968, BIOTINYL_LIPOYL, 1 hit PS00866, CPSASE_1, 1 hit PS00867, CPSASE_2, 1 hit PS50991, PYR_CT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PYC_PICAN | |
Accessioni | Q8X1T3Primary (citable) accession number: Q8X1T3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 31, 2004 |
Last sequence update: | March 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 95 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |