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UniProtKB - Q8WZJ7 (C1TC_SCHPO)
Protein
C-1-tetrahydrofolate synthase, cytoplasmic
Gene
SPBC839.16
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Functioni
Catalytic activityi
: tetrahydrofolate interconversion Pathwayi
This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 193 | NADPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 168 – 170 | NADPBy similarity | 3 | |
Nucleotide bindingi | 374 – 381 | ATPBy similarity | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- formate-tetrahydrofolate ligase activity Source: PomBase
- methenyltetrahydrofolate cyclohydrolase activity Source: PomBase
- methylenetetrahydrofolate dehydrogenase (NADP+) activity Source: PomBase
- single-stranded DNA binding Source: PomBase
GO - Biological processi
- 10-formyltetrahydrofolate biosynthetic process Source: PomBase
- folic acid biosynthetic process Source: PomBase
- histidine biosynthetic process Source: UniProtKB-KW
- methionine biosynthetic process Source: UniProtKB-KW
- one-carbon metabolic process Source: PomBase
- purine nucleobase biosynthetic process Source: PomBase
- purine nucleotide biosynthetic process Source: UniProtKB-KW
- tetrahydrofolate interconversion Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Ligase, Multifunctional enzyme, Oxidoreductase |
Biological process | Amino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis |
Ligand | ATP-binding, NADP, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SPO-196757, Metabolism of folate and pterines |
UniPathwayi | UPA00193 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | ORF Names:SPBC839.16 |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPBC839.16 |
VEuPathDBi | FungiDB:SPBC839.16 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: PomBase
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000315626 | 1 – 937 | C-1-tetrahydrofolate synthase, cytoplasmicAdd BLAST | 937 |
Proteomic databases
MaxQBi | Q8WZJ7 |
PaxDbi | Q8WZJ7 |
PRIDEi | Q8WZJ7 |
PTM databases
iPTMneti | Q8WZJ7 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
BioGRIDi | 277731, 4 interactors |
STRINGi | 4896.SPBC839.16.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 309 | Methylenetetrahydrofolate dehydrogenase and cyclohydrolaseAdd BLAST | 309 | |
Regioni | 50 – 54 | Substrate bindingBy similarity | 5 | |
Regioni | 97 – 99 | Substrate bindingBy similarity | 3 | |
Regioni | 268 – 272 | Substrate bindingBy similarity | 5 | |
Regioni | 310 – 937 | Formyltetrahydrofolate synthetaseAdd BLAST | 628 |
Domaini
This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity.
Sequence similaritiesi
In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.Curated
In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.Curated
Phylogenomic databases
eggNOGi | KOG4230, Eukaryota |
HOGENOMi | CLU_003601_2_0_1 |
InParanoidi | Q8WZJ7 |
OMAi | KVDTYTK |
PhylomeDBi | Q8WZJ7 |
Family and domain databases
CDDi | cd00477, FTHFS, 1 hit cd01080, NAD_bind_m-THF_DH_Cyclohyd, 1 hit |
Gene3Di | 3.40.50.300, 2 hits |
HAMAPi | MF_01543, FTHFS, 1 hit MF_01576, THF_DHG_CYH, 1 hit |
InterProi | View protein in InterPro IPR000559, Formate_THF_ligase IPR020628, Formate_THF_ligase_CS IPR036291, NAD(P)-bd_dom_sf IPR027417, P-loop_NTPase IPR000672, THF_DH/CycHdrlase IPR020630, THF_DH/CycHdrlase_cat_dom IPR020631, THF_DH/CycHdrlase_NAD-bd_dom |
Pfami | View protein in Pfam PF01268, FTHFS, 1 hit PF00763, THF_DHG_CYH, 1 hit PF02882, THF_DHG_CYH_C, 1 hit |
PRINTSi | PR00085, THFDHDRGNASE |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00721, FTHFS_1, 1 hit PS00722, FTHFS_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8WZJ7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALLLEGTSL ARKVREELRE QISSIKSVDP YFNVSLKIIQ VGGREDSNVY
60 70 80 90 100
VRMKTRAANE AGISCEHVNF PEDITEYDLL LAIKGFNEDP TVHGIIVQLP
110 120 130 140 150
LPAHINEQII TEAVAPEKDV DGFCETNLGK LTKREGQPLF TACTPKGIMC
160 170 180 190 200
ILKHYGINVQ GKHAVVIGRS NIVGRPMSIL LEKANATVTL CHSKTESIAD
210 220 230 240 250
IVRTADIVVA AIGIPHFVKA DWLKKGVVAI DVGINSIPDA TKKSGYRLTG
260 270 280 290 300
DIDFENAKEV ASAITPVPGS VGPMTVAMLL QNVVESAVRF RKMSRKRKPT
310 320 330 340 350
LLPLKLQTPV PSDIEIARSQ TPKNIGDLAS EIGIAKSELE FYGSHKAKVN
360 370 380 390 400
LEILQRLAHR RDGHYVVVTG ITPTPFGEGK STLTAGLVQA LSNLDKLAIA
410 420 430 440 450
CVRQPSQGPT FGIKGGAAGG GYSQFIPMEE FNLHLTGDIH AITAATNLLA
460 470 480 490 500
AAIDTRMFHE NTQSDAALYK RLTLVKGNKR EFAPVMFRRL KKLGIDKTNP
510 520 530 540 550
EELTEEEQRK FARLDIEPST ISWNRTLDVN DRFLRKITIG ENPTEKGFTR
560 570 580 590 600
QTGFDLSVAS ECMSVLALAT DLKDMRERLG RMVVASNKSG EPVTADDLGV
610 620 630 640 650
GGALTVLLKD AIKPTLMQTL EGTPALVHAG PFANISIGAS SILADRIALK
660 670 680 690 700
LAGTEVDEDA KKEAGYVVTE AGFASDIGME KFFNIKCRTS GLKPDAIVIV
710 720 730 740 750
ATVQALKLHG GGPPVGPGKP IPEVYKREDV DLVRKGCANL AKHISNARKY
760 770 780 790 800
GLPVVVAINK FSSDSPNEIS AIREEALAAG ATDAVDSNHW AEGGKGALGV
810 820 830 840 850
ARALINACEN VDSEFRLLYD VHEPIEKKIE IIAKEMYGAD GIELSPLAKE
860 870 880 890 900
RLETFTKQGY NNLPICIAKT QYSLSHDPDL KGAPTNFTVP IRDMRLSAGA
910 920 930
GFIYPLAAAI STIPGLPTKP AYYNIDIAEN GDIVGLS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329671 Genomic DNA Translation: CAB46709.1 |
PIRi | T40723 |
RefSeqi | NP_595256.1, NM_001021162.2 |
Genome annotation databases
EnsemblFungii | SPBC839.16.1; SPBC839.16.1:pep; SPBC839.16 |
GeneIDi | 2541217 |
KEGGi | spo:SPBC839.16 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329671 Genomic DNA Translation: CAB46709.1 |
PIRi | T40723 |
RefSeqi | NP_595256.1, NM_001021162.2 |
3D structure databases
SMRi | Q8WZJ7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 277731, 4 interactors |
STRINGi | 4896.SPBC839.16.1 |
PTM databases
iPTMneti | Q8WZJ7 |
Proteomic databases
MaxQBi | Q8WZJ7 |
PaxDbi | Q8WZJ7 |
PRIDEi | Q8WZJ7 |
Genome annotation databases
EnsemblFungii | SPBC839.16.1; SPBC839.16.1:pep; SPBC839.16 |
GeneIDi | 2541217 |
KEGGi | spo:SPBC839.16 |
Organism-specific databases
PomBasei | SPBC839.16 |
VEuPathDBi | FungiDB:SPBC839.16 |
Phylogenomic databases
eggNOGi | KOG4230, Eukaryota |
HOGENOMi | CLU_003601_2_0_1 |
InParanoidi | Q8WZJ7 |
OMAi | KVDTYTK |
PhylomeDBi | Q8WZJ7 |
Enzyme and pathway databases
UniPathwayi | UPA00193 |
Reactomei | R-SPO-196757, Metabolism of folate and pterines |
Miscellaneous databases
PROi | PR:Q8WZJ7 |
Family and domain databases
CDDi | cd00477, FTHFS, 1 hit cd01080, NAD_bind_m-THF_DH_Cyclohyd, 1 hit |
Gene3Di | 3.40.50.300, 2 hits |
HAMAPi | MF_01543, FTHFS, 1 hit MF_01576, THF_DHG_CYH, 1 hit |
InterProi | View protein in InterPro IPR000559, Formate_THF_ligase IPR020628, Formate_THF_ligase_CS IPR036291, NAD(P)-bd_dom_sf IPR027417, P-loop_NTPase IPR000672, THF_DH/CycHdrlase IPR020630, THF_DH/CycHdrlase_cat_dom IPR020631, THF_DH/CycHdrlase_NAD-bd_dom |
Pfami | View protein in Pfam PF01268, FTHFS, 1 hit PF00763, THF_DHG_CYH, 1 hit PF02882, THF_DHG_CYH_C, 1 hit |
PRINTSi | PR00085, THFDHDRGNASE |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00721, FTHFS_1, 1 hit PS00722, FTHFS_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | C1TC_SCHPO | |
Accessioni | Q8WZJ7Primary (citable) accession number: Q8WZJ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | March 1, 2002 | |
Last modified: | February 23, 2022 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families