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Entry version 180 (12 Aug 2020)
Sequence version 1 (01 Mar 2002)
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Protein

Microprocessor complex subunit DGCR8

Gene

DGCR8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal (By similarity).By similarity10 Publications

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

heme1 PublicationNote: Binds 1 heme group per homodimer.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi352Iron (heme axial ligand)1 Publication1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionRNA-binding
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

More...
PathwayCommonsi
Q8WYQ5

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-HSA-203927, MicroRNA (miRNA) biogenesis
R-HSA-8986944, Transcriptional Regulation by MECP2

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Microprocessor complex subunit DGCR8
Alternative name(s):
DiGeorge syndrome critical region 8
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:DGCR8
Synonyms:C22orf12, DGCRK6
ORF Names:LP4941
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 22

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
HostDB:ENSG00000128191.13

Human Gene Nomenclature Database

More...
HGNCi
HGNC:2847, DGCR8

Online Mendelian Inheritance in Man (OMIM)

More...
MIMi
609030, gene

neXtProt; the human protein knowledge platform

More...
neXtProti
NX_Q8WYQ5

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi352C → A or H: Inhibits heme-binding and dimerization. 1 Publication1
Mutagenesisi430C → A: Does not inhibit heme-binding and dimerization. 1 Publication1
Mutagenesisi561 – 565KKLAK → AALAA: Strongly reduces pri-miRNA binding affinity. 1 Publication5
Mutagenesisi568 – 569AA → KK: Reduces pri-miRNA binding affinity and pri-miRNA processing activity. Does not inhibit interaction with DROSHA. When associated with A-676 and S-677, strongly reduces binding affinity and pri-miRNA processing activity. 1 Publication2
Mutagenesisi669 – 673KRVGK → AAVGA: Strongly reduces pri-miRNA binding affinity. 1 Publication5
Mutagenesisi676 – 677AS → KK: Reduces pri-miRNA binding affinity and pri-miRNA processing activity. Slightly inhibits interaction with DROSHA. When associated with A-568 and A-568, strongly reduces binding affinity and pri-miRNA processing activity. 1 Publication2

Organism-specific databases

DisGeNET

More...
DisGeNETi
54487

GeneReviews a resource of expert-authored, peer-reviewed disease descriptions.

More...
GeneReviewsi
DGCR8

MalaCards human disease database

More...
MalaCardsi
DGCR8

Open Targets

More...
OpenTargetsi
ENSG00000128191

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...
PharmGKBi
PA27309

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...
Pharosi
Q8WYQ5, Tbio

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...
BioMutai
DGCR8

Domain mapping of disease mutations (DMDM)

More...
DMDMi
23813990

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000798781 – 773Microprocessor complex subunit DGCR8Add BLAST773

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei35PhosphoserineCombined sources1
Modified residuei92PhosphoserineCombined sources1
Modified residuei95PhosphoserineCombined sources1
Modified residuei271PhosphoserineCombined sources1
Modified residuei275PhosphoserineCombined sources1
Modified residuei279PhosphothreonineBy similarity1
Modified residuei371PhosphothreonineCombined sources1
Modified residuei373PhosphoserineCombined sources1
Modified residuei377PhosphoserineCombined sources1
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki424Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki500Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki707Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
Q8WYQ5

jPOST - Japan Proteome Standard Repository/Database

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jPOSTi
Q8WYQ5

MassIVE - Mass Spectrometry Interactive Virtual Environment

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MassIVEi
Q8WYQ5

MaxQB - The MaxQuant DataBase

More...
MaxQBi
Q8WYQ5

PaxDb, a database of protein abundance averages across all three domains of life

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PaxDbi
Q8WYQ5

PeptideAtlas

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PeptideAtlasi
Q8WYQ5

PRoteomics IDEntifications database

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PRIDEi
Q8WYQ5

ProteomicsDB: a multi-organism proteome resource

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ProteomicsDBi
75188 [Q8WYQ5-1]
75189 [Q8WYQ5-2]
75190 [Q8WYQ5-3]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

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iPTMneti
Q8WYQ5

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

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PhosphoSitePlusi
Q8WYQ5

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSG00000128191, Expressed in cerebellar hemisphere and 222 other tissues

ExpressionAtlas, Differential and Baseline Expression

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ExpressionAtlasi
Q8WYQ5, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
Q8WYQ5, HS

Organism-specific databases

Human Protein Atlas

More...
HPAi
ENSG00000128191, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Monomer; in absence of heme. Homodimer; the association with heme promotes its dimerization (PubMed:17159994).

Component of the microprocessor complex, or pri-miRNA processing protein complex, which is composed of DROSHA and DGCR8 (PubMed:15589161, PubMed:15574589, PubMed:15531877, PubMed:16751099, PubMed:19135890, PubMed:26027739, PubMed:26748718). The microprocessor complex is a heterotrimer; each of the two DROSHA RNase III domains binds one DGCR8 (via C-terminal region) (PubMed:26027739, PubMed:26748718).

Interacts with ILF3, NCL and DROSHA (PubMed:17765891).

Interacts with CPSF3 and ISY1; this interaction is in an RNA dependent manner (By similarity).

Interacts with PUS10; interaction promotes pri-miRNAs processing (PubMed:31819270).

By similarity10 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

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BioGRIDi
119986, 162 interactors

ComplexPortal: manually curated resource of macromolecular complexes

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ComplexPortali
CPX-3080, Microprocessor complex

CORUM comprehensive resource of mammalian protein complexes

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CORUMi
Q8WYQ5

Database of interacting proteins

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DIPi
DIP-29261N

Protein interaction database and analysis system

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IntActi
Q8WYQ5, 118 interactors

Molecular INTeraction database

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MINTi
Q8WYQ5

STRING: functional protein association networks

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STRINGi
9606.ENSP00000263209

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

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RNActi
Q8WYQ5, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1773
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q8WYQ5

Database of comparative protein structure models

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ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

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PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

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EvolutionaryTracei
Q8WYQ5

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini301 – 334WWPROSITE-ProRule annotationAdd BLAST34
Domaini511 – 578DRBM 1PROSITE-ProRule annotationAdd BLAST68
Domaini620 – 685DRBM 2PROSITE-ProRule annotationAdd BLAST66

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 342Necessary for interaction with NCL1 PublicationAdd BLAST342
Regioni1 – 275Necessary for nuclear localization and retentionAdd BLAST275
Regioni276 – 751Necessary for heme-binding and pri-miRNA processingAdd BLAST476
Regioni701 – 773Interaction with DROSHA1 PublicationAdd BLAST73

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

Both DRBM domains are required for efficient binding to pri-miRNA. The region between residues 276 and 498 has an autoinhibitory function on pri-miRNA processing activity.

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
KOG4334, Eukaryota

Ensembl GeneTree

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GeneTreei
ENSGT00390000015977

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

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HOGENOMi
CLU_017211_3_0_1

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q8WYQ5

KEGG Orthology (KO)

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KOi
K18419

Identification of Orthologs from Complete Genome Data

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OMAi
MMENRAC

Database of Orthologous Groups

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OrthoDBi
855874at2759

Database for complete collections of gene phylogenies

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PhylomeDBi
Q8WYQ5

TreeFam database of animal gene trees

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TreeFami
TF324256

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00048, DSRM, 1 hit
cd00201, WW, 1 hit

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR040375, DGCR8
IPR014720, dsRBD_dom
IPR001202, WW_dom
IPR036020, WW_dom_sf

The PANTHER Classification System

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PANTHERi
PTHR13482, PTHR13482, 1 hit

Pfam protein domain database

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Pfami
View protein in Pfam
PF00035, dsrm, 2 hits

Simple Modular Architecture Research Tool; a protein domain database

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SMARTi
View protein in SMART
SM00358, DSRM, 2 hits
SM00456, WW, 1 hit

Superfamily database of structural and functional annotation

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SUPFAMi
SSF51045, SSF51045, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50137, DS_RBD, 1 hit
PS50020, WW_DOMAIN_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 3 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8WYQ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
METDESPSPL PCGPAGEAVM ESRARPFQAL PREQSPPPPL QTSSGAEVMD
60 70 80 90 100
VGSGGDGQSE LPAEDPFNFY GASLLSKGSF SKGRLLIDPN CSGHSPRTAR
110 120 130 140 150
HAPAVRKFSP DLKLLKDVKI SVSFTESCRS KDRKVLYTGA ERDVRAECGL
160 170 180 190 200
LLSPVSGDVH ACPFGGSVGD GVGIGGESAD KKDEENELDQ EKRVEYAVLD
210 220 230 240 250
ELEDFTDNLE LDEEGAGGFT AKAIVQRDRV DEEALNFPYE DDFDNDVDAL
260 270 280 290 300
LEEGLCAPKK RRTEEKYGGD SDHPSDGETS VQPMMTKIKT VLKSRGRPPT
310 320 330 340 350
EPLPDGWIMT FHNSGVPVYL HRESRVVTWS RPYFLGTGSI RKHDPPLSSI
360 370 380 390 400
PCLHYKKMKD NEEREQSSDL TPSGDVSPVK PLSRSAELEF PLDEPDSMGA
410 420 430 440 450
DPGPPDEKDP LGAEAAPGAL GQVKAKVEVC KDESVDLEEF RSYLEKRFDF
460 470 480 490 500
EQVTVKKFRT WAERRQFNRE MKRKQAESER PILPANQKLI TLSVQDAPTK
510 520 530 540 550
KEFVINPNGK SEVCILHEYM QRVLKVRPVY NFFECENPSE PFGASVTIDG
560 570 580 590 600
VTYGSGTASS KKLAKNKAAR ATLEILIPDF VKQTSEEKPK DSEELEYFNH
610 620 630 640 650
ISIEDSRVYE LTSKAGLLSP YQILHECLKR NHGMGDTSIK FEVVPGKNQK
660 670 680 690 700
SEYVMACGKH TVRGWCKNKR VGKQLASQKI LQLLHPHVKN WGSLLRMYGR
710 720 730 740 750
ESSKMVKQET SDKSVIELQQ YAKKNKPNLH ILSKLQEEMK RLAEEREETR
760 770
KKPKMSIVAS AQPGGEPLCT VDV
Length:773
Mass (Da):86,045
Last modified:March 1, 2002 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i72D962BBE32890EC
GO
Isoform 2 (identifier: Q8WYQ5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     303-304: LP → VL
     305-773: Missing.

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Length:304
Mass (Da):32,831
Checksum:iFA7800BFE8FD462A
GO
Isoform 3 (identifier: Q8WYQ5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     536-568: Missing.

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Length:740
Mass (Da):82,750
Checksum:i5EEDDD1111DE7DC6
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JSD5C9JSD5_HUMAN
Microprocessor complex subunit DGCR...
DGCR8
125Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAO86726 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAB15165 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAB15238 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti241 – 247DDFDNDV → VCWQPLL in AAF82263 (Ref. 6) Curated7
Sequence conflicti274P → L in BAB15165 (PubMed:14702039).Curated1
Sequence conflicti343H → Y in BAB15165 (PubMed:14702039).Curated1
Sequence conflicti706V → A in BAB15165 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_050952174I → V. Corresponds to variant dbSNP:rs35987994Ensembl.1
Natural variantiVAR_050953725N → D. Corresponds to variant dbSNP:rs11546015Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_003847303 – 304LP → VL in isoform 2. 2 Publications2
Alternative sequenceiVSP_003848305 – 773Missing in isoform 2. 2 PublicationsAdd BLAST469
Alternative sequenceiVSP_012707536 – 568Missing in isoform 3. 1 PublicationAdd BLAST33

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
AB050770 Genomic DNA Translation: BAB83032.1
CR456356 mRNA Translation: CAG30242.1
AK025539 mRNA Translation: BAB15165.1 Different initiation.
AK025780 mRNA Translation: BAB15238.1 Different initiation.
AK313357 mRNA Translation: BAG36158.1
AC006547 Genomic DNA No translation available.
BC009323 mRNA Translation: AAH09323.2
BC009984 mRNA Translation: AAH09984.1
BC078147 mRNA Translation: AAH78147.1
AF165527 mRNA Translation: AAF82263.1
BX649187 mRNA Translation: CAE46205.2
AY189282 mRNA Translation: AAO86726.1 Different initiation.

The Consensus CDS (CCDS) project

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CCDSi
CCDS13773.1 [Q8WYQ5-1]
CCDS54501.1 [Q8WYQ5-3]

NCBI Reference Sequences

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RefSeqi
NP_001177255.1, NM_001190326.1 [Q8WYQ5-3]
NP_073557.3, NM_022720.6 [Q8WYQ5-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

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Ensembli
ENST00000351989; ENSP00000263209; ENSG00000128191 [Q8WYQ5-1]
ENST00000383024; ENSP00000372488; ENSG00000128191 [Q8WYQ5-3]
ENST00000407755; ENSP00000384726; ENSG00000128191 [Q8WYQ5-3]

Database of genes from NCBI RefSeq genomes

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GeneIDi
54487

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
hsa:54487

UCSC genome browser

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UCSCi
uc002zri.4, human [Q8WYQ5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB050770 Genomic DNA Translation: BAB83032.1
CR456356 mRNA Translation: CAG30242.1
AK025539 mRNA Translation: BAB15165.1 Different initiation.
AK025780 mRNA Translation: BAB15238.1 Different initiation.
AK313357 mRNA Translation: BAG36158.1
AC006547 Genomic DNA No translation available.
BC009323 mRNA Translation: AAH09323.2
BC009984 mRNA Translation: AAH09984.1
BC078147 mRNA Translation: AAH78147.1
AF165527 mRNA Translation: AAF82263.1
BX649187 mRNA Translation: CAE46205.2
AY189282 mRNA Translation: AAO86726.1 Different initiation.
CCDSiCCDS13773.1 [Q8WYQ5-1]
CCDS54501.1 [Q8WYQ5-3]
RefSeqiNP_001177255.1, NM_001190326.1 [Q8WYQ5-3]
NP_073557.3, NM_022720.6 [Q8WYQ5-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

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PDBei

Protein Data Bank RCSB

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RCSB PDBi

Protein Data Bank Japan

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PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X47NMR-A502-586[»]
2YT4X-ray2.60A493-720[»]
3LE4X-ray1.70A275-353[»]
5B16X-ray3.20B/C728-750[»]
6LXDelectron microscopy3.90B/C1-773[»]
6LXEelectron microscopy4.20B/C1-773[»]
6V5Belectron microscopy3.70B/C223-751[»]
6V5Celectron microscopy4.40B/C223-751[»]
SMRiQ8WYQ5
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi119986, 162 interactors
ComplexPortaliCPX-3080, Microprocessor complex
CORUMiQ8WYQ5
DIPiDIP-29261N
IntActiQ8WYQ5, 118 interactors
MINTiQ8WYQ5
STRINGi9606.ENSP00000263209

PTM databases

iPTMnetiQ8WYQ5
PhosphoSitePlusiQ8WYQ5

Polymorphism and mutation databases

BioMutaiDGCR8
DMDMi23813990

Proteomic databases

EPDiQ8WYQ5
jPOSTiQ8WYQ5
MassIVEiQ8WYQ5
MaxQBiQ8WYQ5
PaxDbiQ8WYQ5
PeptideAtlasiQ8WYQ5
PRIDEiQ8WYQ5
ProteomicsDBi75188 [Q8WYQ5-1]
75189 [Q8WYQ5-2]
75190 [Q8WYQ5-3]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

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Antibodypediai
7887, 301 antibodies

The DNASU plasmid repository

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DNASUi
54487

Genome annotation databases

EnsembliENST00000351989; ENSP00000263209; ENSG00000128191 [Q8WYQ5-1]
ENST00000383024; ENSP00000372488; ENSG00000128191 [Q8WYQ5-3]
ENST00000407755; ENSP00000384726; ENSG00000128191 [Q8WYQ5-3]
GeneIDi54487
KEGGihsa:54487
UCSCiuc002zri.4, human [Q8WYQ5-1]

Organism-specific databases

Comparative Toxicogenomics Database

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CTDi
54487
DisGeNETi54487
EuPathDBiHostDB:ENSG00000128191.13

GeneCards: human genes, protein and diseases

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GeneCardsi
DGCR8
GeneReviewsiDGCR8
HGNCiHGNC:2847, DGCR8
HPAiENSG00000128191, Low tissue specificity
MalaCardsiDGCR8
MIMi609030, gene
neXtProtiNX_Q8WYQ5
OpenTargetsiENSG00000128191
PharmGKBiPA27309

GenAtlas: human gene database

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GenAtlasi
Search...

Phylogenomic databases

eggNOGiKOG4334, Eukaryota
GeneTreeiENSGT00390000015977
HOGENOMiCLU_017211_3_0_1
InParanoidiQ8WYQ5
KOiK18419
OMAiMMENRAC
OrthoDBi855874at2759
PhylomeDBiQ8WYQ5
TreeFamiTF324256

Enzyme and pathway databases

PathwayCommonsiQ8WYQ5
ReactomeiR-HSA-203927, MicroRNA (miRNA) biogenesis
R-HSA-8986944, Transcriptional Regulation by MECP2

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

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BioGRID-ORCSi
54487, 326 hits in 883 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
DGCR8, human
EvolutionaryTraceiQ8WYQ5

The Gene Wiki collection of pages on human genes and proteins

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GeneWikii
DGCR8_(gene)

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
54487
PharosiQ8WYQ5, Tbio

Protein Ontology

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PROi
PR:Q8WYQ5
RNActiQ8WYQ5, protein

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSG00000128191, Expressed in cerebellar hemisphere and 222 other tissues
ExpressionAtlasiQ8WYQ5, baseline and differential
GenevisibleiQ8WYQ5, HS

Family and domain databases

CDDicd00048, DSRM, 1 hit
cd00201, WW, 1 hit
InterProiView protein in InterPro
IPR040375, DGCR8
IPR014720, dsRBD_dom
IPR001202, WW_dom
IPR036020, WW_dom_sf
PANTHERiPTHR13482, PTHR13482, 1 hit
PfamiView protein in Pfam
PF00035, dsrm, 2 hits
SMARTiView protein in SMART
SM00358, DSRM, 2 hits
SM00456, WW, 1 hit
SUPFAMiSSF51045, SSF51045, 1 hit
PROSITEiView protein in PROSITE
PS50137, DS_RBD, 1 hit
PS50020, WW_DOMAIN_2, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiDGCR8_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8WYQ5
Secondary accession number(s): B2R8G1
, Q6DCB2, Q6MZE9, Q6Y2L0, Q96G39, Q96GP8, Q9H6L8, Q9H6T7, Q9NRW2
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: March 1, 2002
Last modified: August 12, 2020
This is version 180 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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