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UniProtKB - Q8WYQ5 (DGCR8_HUMAN)

Entry version 188 (25 May 2022)
Sequence version 1 (01 Mar 2002)
Previous versions | rss
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Protein

Microprocessor complex subunit DGCR8

Gene

DGCR8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718).

The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994).

Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998).

Involved in the silencing of embryonic stem cell self-renewal (By similarity).

By similarity10 Publications

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

heme1 PublicationNote: Binds 1 heme group per homodimer.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi352Iron (heme axial ligand)1 Publication1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionRNA-binding
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		Q8WYQ5

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-203927, MicroRNA (miRNA) biogenesis
R-HSA-8986944, Transcriptional Regulation by MECP2

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...SignaLinki		Q8WYQ5

SIGNOR Signaling Network Open Resource

More...SIGNORi		Q8WYQ5

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Microprocessor complex subunit DGCR8
Alternative name(s):
DiGeorge syndrome critical region 8
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:DGCR8
Synonyms:C22orf12, DGCRK6
ORF Names:LP4941
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 22

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:2847, DGCR8

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		609030, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_Q8WYQ5

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000128191

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi352C → A or H: Inhibits heme-binding and dimerization. 1 Publication1
Mutagenesisi430C → A: Does not inhibit heme-binding and dimerization. 1 Publication1
Mutagenesisi561 – 565KKLAK → AALAA: Strongly reduces pri-miRNA binding affinity. 1 Publication5
Mutagenesisi568 – 569AA → KK: Reduces pri-miRNA binding affinity and pri-miRNA processing activity. Does not inhibit interaction with DROSHA. When associated with A-676 and S-677, strongly reduces binding affinity and pri-miRNA processing activity. 1 Publication2
Mutagenesisi669 – 673KRVGK → AAVGA: Strongly reduces pri-miRNA binding affinity. 1 Publication5
Mutagenesisi676 – 677AS → KK: Reduces pri-miRNA binding affinity and pri-miRNA processing activity. Slightly inhibits interaction with DROSHA. When associated with A-568 and A-568, strongly reduces binding affinity and pri-miRNA processing activity. 1 Publication2

Organism-specific databases

DisGeNET

More...DisGeNETi		54487

GeneReviews a resource of expert-authored, peer-reviewed disease descriptions.

More...GeneReviewsi		DGCR8

MalaCards human disease database

More...MalaCardsi		DGCR8

Open Targets

More...OpenTargetsi		ENSG00000128191

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA27309

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		Q8WYQ5, Tbio

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		DGCR8

Domain mapping of disease mutations (DMDM)

More...DMDMi		23813990

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000798781 – 773Microprocessor complex subunit DGCR8Add BLAST773

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei35PhosphoserineCombined sources1
Modified residuei92PhosphoserineCombined sources1
Modified residuei95PhosphoserineCombined sources1
Modified residuei271PhosphoserineCombined sources1
Modified residuei275PhosphoserineCombined sources1
Modified residuei279PhosphothreonineBy similarity1
Modified residuei371PhosphothreonineCombined sources1
Modified residuei373PhosphoserineCombined sources1
Modified residuei377PhosphoserineCombined sources1
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki424Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki500Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki707Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		Q8WYQ5

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q8WYQ5

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		Q8WYQ5

MaxQB - The MaxQuant DataBase

More...MaxQBi		Q8WYQ5

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q8WYQ5

PeptideAtlas

More...PeptideAtlasi		Q8WYQ5

PRoteomics IDEntifications database

More...PRIDEi		Q8WYQ5

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		75188 [Q8WYQ5-1]
75189 [Q8WYQ5-2]
75190 [Q8WYQ5-3]

PTM databases

GlyGen: Computational and Informatics Resources for Glycoscience

More...GlyGeni		Q8WYQ5, 1 site, 1 O-linked glycan (1 site)

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q8WYQ5

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q8WYQ5

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000128191, Expressed in cerebellar hemisphere and 223 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		Q8WYQ5, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q8WYQ5, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000128191, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Monomer; in absence of heme. Homodimer; the association with heme promotes its dimerization (PubMed:17159994).

Component of the microprocessor complex, or pri-miRNA processing protein complex, which is composed of DROSHA and DGCR8 (PubMed:15589161, PubMed:15574589, PubMed:15531877, PubMed:16751099, PubMed:19135890, PubMed:26027739, PubMed:26748718). The microprocessor complex is a heterotrimer; each of the two DROSHA RNase III domains binds one DGCR8 (via C-terminal region) (PubMed:26027739, PubMed:26748718).

Interacts with ILF3, NCL and DROSHA (PubMed:17765891).

Interacts with CPSF3 and ISY1; this interaction is in an RNA dependent manner (By similarity).

Interacts with PUS10; interaction promotes pri-miRNAs processing (PubMed:31819270).

By similarity10 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		119986, 231 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-3080, Microprocessor complex

CORUM comprehensive resource of mammalian protein complexes

More...CORUMi		Q8WYQ5

Database of interacting proteins

More...DIPi		DIP-29261N

Protein interaction database and analysis system

More...IntActi		Q8WYQ5, 118 interactors

Molecular INTeraction database

More...MINTi		Q8WYQ5

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000263209

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q8WYQ5, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1773
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

AlphaFold Protein Structure Database

More...AlphaFoldDBi		Q8WYQ5

Biological Magnetic Resonance Data Bank

More...BMRBi		Q8WYQ5

Small Angle Scattering Biological Data Bank

More...SASBDBi		Q8WYQ5

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q8WYQ5

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		Q8WYQ5

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini301 – 334WWPROSITE-ProRule annotationAdd BLAST34
Domaini511 – 578DRBM 1PROSITE-ProRule annotationAdd BLAST68
Domaini620 – 685DRBM 2PROSITE-ProRule annotationAdd BLAST66

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 342Necessary for interaction with NCL1 PublicationAdd BLAST342
Regioni1 – 275Necessary for nuclear localization and retentionAdd BLAST275
Regioni1 – 63DisorderedSequence analysisAdd BLAST63
Regioni261 – 283DisorderedSequence analysisAdd BLAST23
Regioni276 – 751Necessary for heme-binding and pri-miRNA processingAdd BLAST476
Regioni360 – 414DisorderedSequence analysisAdd BLAST55
Regioni701 – 773Interaction with DROSHA1 PublicationAdd BLAST73
Regioni742 – 773DisorderedSequence analysisAdd BLAST32

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

Both DRBM domains are required for efficient binding to pri-miRNA. The region between residues 276 and 498 has an autoinhibitory function on pri-miRNA processing activity.

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG4334, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00390000015977

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_017211_3_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q8WYQ5

Identification of Orthologs from Complete Genome Data

More...OMAi		QDYKADT

Database of Orthologous Groups

More...OrthoDBi		855874at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q8WYQ5

TreeFam database of animal gene trees

More...TreeFami		TF324256

Family and domain databases

Conserved Domains Database

More...CDDi		cd00201, WW, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR040375, DGCR8
IPR014720, dsRBD_dom
IPR001202, WW_dom
IPR036020, WW_dom_sf

The PANTHER Classification System

More...PANTHERi		PTHR13482, PTHR13482, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00035, dsrm, 2 hits

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00358, DSRM, 2 hits
SM00456, WW, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF51045, SSF51045, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS50137, DS_RBD, 1 hit
PS50020, WW_DOMAIN_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 3 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8WYQ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <p><strong>What is the canonical sequence?</strong><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
METDESPSPL PCGPAGEAVM ESRARPFQAL PREQSPPPPL QTSSGAEVMD 
        60         70         80         90        100
VGSGGDGQSE LPAEDPFNFY GASLLSKGSF SKGRLLIDPN CSGHSPRTAR 
       110        120        130        140        150
HAPAVRKFSP DLKLLKDVKI SVSFTESCRS KDRKVLYTGA ERDVRAECGL 
       160        170        180        190        200
LLSPVSGDVH ACPFGGSVGD GVGIGGESAD KKDEENELDQ EKRVEYAVLD 
       210        220        230        240        250
ELEDFTDNLE LDEEGAGGFT AKAIVQRDRV DEEALNFPYE DDFDNDVDAL 
       260        270        280        290        300
LEEGLCAPKK RRTEEKYGGD SDHPSDGETS VQPMMTKIKT VLKSRGRPPT 
       310        320        330        340        350
EPLPDGWIMT FHNSGVPVYL HRESRVVTWS RPYFLGTGSI RKHDPPLSSI 
       360        370        380        390        400
PCLHYKKMKD NEEREQSSDL TPSGDVSPVK PLSRSAELEF PLDEPDSMGA 
       410        420        430        440        450
DPGPPDEKDP LGAEAAPGAL GQVKAKVEVC KDESVDLEEF RSYLEKRFDF 
       460        470        480        490        500
EQVTVKKFRT WAERRQFNRE MKRKQAESER PILPANQKLI TLSVQDAPTK 
       510        520        530        540        550
KEFVINPNGK SEVCILHEYM QRVLKVRPVY NFFECENPSE PFGASVTIDG 
       560        570        580        590        600
VTYGSGTASS KKLAKNKAAR ATLEILIPDF VKQTSEEKPK DSEELEYFNH 
       610        620        630        640        650
ISIEDSRVYE LTSKAGLLSP YQILHECLKR NHGMGDTSIK FEVVPGKNQK 
       660        670        680        690        700
SEYVMACGKH TVRGWCKNKR VGKQLASQKI LQLLHPHVKN WGSLLRMYGR 
       710        720        730        740        750
ESSKMVKQET SDKSVIELQQ YAKKNKPNLH ILSKLQEEMK RLAEEREETR 
       760        770 
KKPKMSIVAS AQPGGEPLCT VDV
Length:773
Mass (Da):86,045
Last modified:March 1, 2002 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i72D962BBE32890EC
GO
Isoform 2 (identifier: Q8WYQ5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     303-304: LP → VL
     305-773: Missing.

Show »
Length:304
Mass (Da):32,831
Checksum:iFA7800BFE8FD462A
GO
Isoform 3 (identifier: Q8WYQ5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     536-568: Missing.

Show »
Length:740
Mass (Da):82,750
Checksum:i5EEDDD1111DE7DC6
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JSD5C9JSD5_HUMAN
Microprocessor complex subunit DGCR...
DGCR8
125Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAO86726 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAB15165 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAB15238 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti241 – 247DDFDNDV → VCWQPLL in AAF82263 (Ref. 6) Curated7
Sequence conflicti274P → L in BAB15165 (PubMed:14702039).Curated1
Sequence conflicti343H → Y in BAB15165 (PubMed:14702039).Curated1
Sequence conflicti706V → A in BAB15165 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_050952174I → V. Corresponds to variant dbSNP:rs35987994Ensembl.1
Natural variantiVAR_050953725N → D. Corresponds to variant dbSNP:rs11546015Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_003847303 – 304LP → VL in isoform 2. 2 Publications2
Alternative sequenceiVSP_003848305 – 773Missing in isoform 2. 2 PublicationsAdd BLAST469
Alternative sequenceiVSP_012707536 – 568Missing in isoform 3. 1 PublicationAdd BLAST33

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AB050770 Genomic DNA Translation: BAB83032.1
CR456356 mRNA Translation: CAG30242.1
AK025539 mRNA Translation: BAB15165.1 Different initiation.
AK025780 mRNA Translation: BAB15238.1 Different initiation.
AK313357 mRNA Translation: BAG36158.1
AC006547 Genomic DNA No translation available.
BC009323 mRNA Translation: AAH09323.2
BC009984 mRNA Translation: AAH09984.1
BC078147 mRNA Translation: AAH78147.1
AF165527 mRNA Translation: AAF82263.1
BX649187 mRNA Translation: CAE46205.2
AY189282 mRNA Translation: AAO86726.1 Different initiation.

The Consensus CDS (CCDS) project

More...CCDSi		CCDS13773.1 [Q8WYQ5-1]
CCDS54501.1 [Q8WYQ5-3]

NCBI Reference Sequences

More...RefSeqi		NP_001177255.1, NM_001190326.1 [Q8WYQ5-3]
NP_073557.3, NM_022720.6 [Q8WYQ5-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000351989.8; ENSP00000263209.3; ENSG00000128191.16
ENST00000383024.6; ENSP00000372488.2; ENSG00000128191.16 [Q8WYQ5-3]
ENST00000407755.1; ENSP00000384726.1; ENSG00000128191.16 [Q8WYQ5-3]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		54487

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:54487

Matched Annotation from NCBI and EMBL-EBI (MANE) - Phase one

More...MANE-Selecti		ENST00000351989.8; ENSP00000263209.3; NM_022720.7; NP_073557.3

UCSC genome browser

More...UCSCi		uc002zri.4, human [Q8WYQ5-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB050770 Genomic DNA Translation: BAB83032.1
CR456356 mRNA Translation: CAG30242.1
AK025539 mRNA Translation: BAB15165.1 Different initiation.
AK025780 mRNA Translation: BAB15238.1 Different initiation.
AK313357 mRNA Translation: BAG36158.1
AC006547 Genomic DNA No translation available.
BC009323 mRNA Translation: AAH09323.2
BC009984 mRNA Translation: AAH09984.1
BC078147 mRNA Translation: AAH78147.1
AF165527 mRNA Translation: AAF82263.1
BX649187 mRNA Translation: CAE46205.2
AY189282 mRNA Translation: AAO86726.1 Different initiation.
CCDSiCCDS13773.1 [Q8WYQ5-1]
CCDS54501.1 [Q8WYQ5-3]
RefSeqiNP_001177255.1, NM_001190326.1 [Q8WYQ5-3]
NP_073557.3, NM_022720.6 [Q8WYQ5-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X47NMR-A502-586[»]
2YT4X-ray2.60A493-720[»]
3LE4X-ray1.70A275-353[»]
5B16X-ray3.20B/C728-750[»]
6LXDelectron microscopy3.90B/C1-773[»]
6LXEelectron microscopy4.20B/C1-773[»]
6V5Belectron microscopy3.70B/C223-751[»]
6V5Celectron microscopy4.40B/C223-751[»]
7CNCX-ray1.60B96-126[»]
AlphaFoldDBiQ8WYQ5
BMRBiQ8WYQ5
SASBDBiQ8WYQ5
SMRiQ8WYQ5
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi119986, 231 interactors
ComplexPortaliCPX-3080, Microprocessor complex
CORUMiQ8WYQ5
DIPiDIP-29261N
IntActiQ8WYQ5, 118 interactors
MINTiQ8WYQ5
STRINGi9606.ENSP00000263209

PTM databases

GlyGeniQ8WYQ5, 1 site, 1 O-linked glycan (1 site)
iPTMnetiQ8WYQ5
PhosphoSitePlusiQ8WYQ5

Genetic variation databases

BioMutaiDGCR8
DMDMi23813990

Proteomic databases

EPDiQ8WYQ5
jPOSTiQ8WYQ5
MassIVEiQ8WYQ5
MaxQBiQ8WYQ5
PaxDbiQ8WYQ5
PeptideAtlasiQ8WYQ5
PRIDEiQ8WYQ5
ProteomicsDBi75188 [Q8WYQ5-1]
75189 [Q8WYQ5-2]
75190 [Q8WYQ5-3]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		7887, 317 antibodies from 35 providers

The DNASU plasmid repository

More...DNASUi		54487

Genome annotation databases

EnsembliENST00000351989.8; ENSP00000263209.3; ENSG00000128191.16
ENST00000383024.6; ENSP00000372488.2; ENSG00000128191.16 [Q8WYQ5-3]
ENST00000407755.1; ENSP00000384726.1; ENSG00000128191.16 [Q8WYQ5-3]
GeneIDi54487
KEGGihsa:54487
MANE-SelectiENST00000351989.8; ENSP00000263209.3; NM_022720.7; NP_073557.3
UCSCiuc002zri.4, human [Q8WYQ5-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		54487
DisGeNETi54487

GeneCards: human genes, protein and diseases

More...GeneCardsi		DGCR8
GeneReviewsiDGCR8
HGNCiHGNC:2847, DGCR8
HPAiENSG00000128191, Low tissue specificity
MalaCardsiDGCR8
MIMi609030, gene
neXtProtiNX_Q8WYQ5
OpenTargetsiENSG00000128191
PharmGKBiPA27309
VEuPathDBiHostDB:ENSG00000128191

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG4334, Eukaryota
GeneTreeiENSGT00390000015977
HOGENOMiCLU_017211_3_0_1
InParanoidiQ8WYQ5
OMAiQDYKADT
OrthoDBi855874at2759
PhylomeDBiQ8WYQ5
TreeFamiTF324256

Enzyme and pathway databases

PathwayCommonsiQ8WYQ5
ReactomeiR-HSA-203927, MicroRNA (miRNA) biogenesis
R-HSA-8986944, Transcriptional Regulation by MECP2
SignaLinkiQ8WYQ5
SIGNORiQ8WYQ5

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		54487, 362 hits in 1093 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		DGCR8, human
EvolutionaryTraceiQ8WYQ5

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		DGCR8_(gene)

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		54487
PharosiQ8WYQ5, Tbio

Protein Ontology

More...PROi		PR:Q8WYQ5
RNActiQ8WYQ5, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000128191, Expressed in cerebellar hemisphere and 223 other tissues
ExpressionAtlasiQ8WYQ5, baseline and differential
GenevisibleiQ8WYQ5, HS

Family and domain databases

CDDicd00201, WW, 1 hit
InterProiView protein in InterPro
IPR040375, DGCR8
IPR014720, dsRBD_dom
IPR001202, WW_dom
IPR036020, WW_dom_sf
PANTHERiPTHR13482, PTHR13482, 1 hit
PfamiView protein in Pfam
PF00035, dsrm, 2 hits
SMARTiView protein in SMART
SM00358, DSRM, 2 hits
SM00456, WW, 1 hit
SUPFAMiSSF51045, SSF51045, 1 hit
PROSITEiView protein in PROSITE
PS50137, DS_RBD, 1 hit
PS50020, WW_DOMAIN_2, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiDGCR8_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8WYQ5
Secondary accession number(s): B2R8G1
, Q6DCB2, Q6MZE9, Q6Y2L0, Q96G39, Q96GP8, Q9H6L8, Q9H6T7, Q9NRW2
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: March 1, 2002
Last modified: May 25, 2022
This is version 188 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with genetic variants
    List of human entries with genetic variants
  3. Human variants curated from literature reports
    Index of human variants curated from literature reports
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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