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Protein

Acyl-coenzyme A thioesterase 12

Gene

ACOT12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes acetyl-CoA to acetate and CoA.1 Publication

Catalytic activityi

Acetyl-CoA + H2O = CoA + acetate.

Activity regulationi

Inhibited by ADP. Active in the presence of ATP.1 Publication

Pathwayi: pyruvate metabolism

This protein is involved in the pathway pyruvate metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway pyruvate metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei144Coenzyme A1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Serine esterase
Biological processFatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.2.1 2681
ReactomeiR-HSA-77289 Mitochondrial Fatty Acid Beta-Oxidation
UniPathwayi
UPA00231

Protein family/group databases

TCDBi4.C.3.1.2 the acyl-coa thioesterase (acot) family

Chemistry databases

SwissLipidsiSLP:000001187

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A thioesterase 12 (EC:3.1.2.1)
Short name:
Acyl-CoA thioesterase 12
Alternative name(s):
Acyl-CoA thioester hydrolase 12
Cytoplasmic acetyl-CoA hydrolase 1
Short name:
CACH-1
Short name:
hCACH-1
START domain-containing protein 15
Short name:
StARD15
Gene namesi
Name:ACOT12
Synonyms:CACH, CACH1, STARD15
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000172497.8
HGNCiHGNC:24436 ACOT12
MIMi614315 gene
neXtProtiNX_Q8WYK0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi134526
OpenTargetsiENSG00000172497
PharmGKBiPA142672657

Polymorphism and mutation databases

BioMutaiACOT12
DMDMi25008183

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000538091 – 555Acyl-coenzyme A thioesterase 12Add BLAST555

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33N6-succinyllysineBy similarity1
Modified residuei159N6-succinyllysineBy similarity1
Modified residuei228N6-succinyllysineBy similarity1

Proteomic databases

PaxDbiQ8WYK0
PeptideAtlasiQ8WYK0
PRIDEiQ8WYK0
ProteomicsDBi75162

PTM databases

iPTMnetiQ8WYK0
PhosphoSitePlusiQ8WYK0

Expressioni

Gene expression databases

BgeeiENSG00000172497 Expressed in 49 organ(s), highest expression level in right lobe of liver
CleanExiHS_ACOT12
GenevisibleiQ8WYK0 HS

Organism-specific databases

HPAiHPA037723
HPA037724

Interactioni

Subunit structurei

Homodimer or homotetramer.By similarity

Protein-protein interaction databases

BioGridi126405, 6 interactors
IntActiQ8WYK0, 9 interactors
STRINGi9606.ENSP00000303246

Structurei

Secondary structure

1555
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8WYK0
SMRiQ8WYK0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8WYK0

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 117HotDog ACOT-type 1PROSITE-ProRule annotationAdd BLAST113
Domaini179 – 294HotDog ACOT-type 2PROSITE-ProRule annotationAdd BLAST116
Domaini340 – 549STARTPROSITE-ProRule annotationAdd BLAST210

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni53 – 55Coenzyme A binding3
Regioni82 – 84Coenzyme A binding3
Regioni234 – 236Coenzyme A binding3

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2763 Eukaryota
COG1607 LUCA
GeneTreeiENSGT00760000119297
HOGENOMiHOG000232032
HOVERGENiHBG023847
InParanoidiQ8WYK0
KOiK01067
OMAiRPLWDPH
OrthoDBiEOG091G06LZ
PhylomeDBiQ8WYK0
TreeFamiTF328368

Family and domain databases

Gene3Di3.30.530.20, 1 hit
InterProiView protein in InterPro
IPR033120 HOTDOG_ACOT
IPR029069 HotDog_dom_sf
IPR023393 START-like_dom_sf
IPR002913 START_lipid-bd_dom
IPR006683 Thioestr_dom
PfamiView protein in Pfam
PF03061 4HBT, 2 hits
PF01852 START, 1 hit
SUPFAMiSSF54637 SSF54637, 2 hits
PROSITEiView protein in PROSITE
PS51770 HOTDOG_ACOT, 2 hits
PS50848 START, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q8WYK0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MERPAPGEVV MSQAIQPAHA TARGELSAGQ LLKWIDTTAC LAAEKHAGVS
60 70 80 90 100
CVTASVDDIQ FEETARVGQV ITIKAKVTRA FSTSMEISIK VMVQDMLTGI
110 120 130 140 150
EKLVSVAFST FVAKPVGKEK IHLKPVTLLT EQDHVEHNLA AERRKVRLQH
160 170 180 190 200
EDTFNNLMKE SSKFDDLIFD EEEGAVSTRG TSVQSIELVL PPHANHHGNT
210 220 230 240 250
FGGQIMAWME TVATISASRL CWAHPFLKSV DMFKFRGPST VGDRLVFTAI
260 270 280 290 300
VNNTFQTCVE VGVRVEAFDC QEWAEGRGRH INSAFLIYNA ADDKENLITF
310 320 330 340 350
PRIQPISKDD FRRYRGAIAR KRIRLGRKYV ISHKEEVPLC IHWDISKQAS
360 370 380 390 400
LSDSNVEALK KLAAKRGWEV TSTVEKIKIY TLEEHDVLSV WVEKHVGSPA
410 420 430 440 450
HLAYRLLSDF TKRPLWDPHF VSCEVIDWVS EDDQLYHITC PILNDDKPKD
460 470 480 490 500
LVVLVSRRKP LKDGNTYTVA VKSVILPSVP PSPQYIRSEI ICAGFLIHAI
510 520 530 540 550
DSNSCIVSYF NHMSASILPY FAGNLGGWSK SIEETAASCI QFLENPPDDG

FVSTF
Length:555
Mass (Da):62,034
Last modified:March 1, 2002 - v1
Checksum:i707560D55504732C
GO
Isoform 2 (identifier: Q8WYK0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     166-167: DL → GQ
     168-555: Missing.

Note: No experimental confirmation available.
Show »
Length:167
Mass (Da):18,327
Checksum:i58CB03F09579E5A4
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_064691190L → H Found in a clear cell renal carcinoma case; somatic mutation. 1 Publication1
Natural variantiVAR_048192230V → I. Corresponds to variant dbSNP:rs34607174Ensembl.1
Natural variantiVAR_048193403A → T. Corresponds to variant dbSNP:rs10371Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_055785166 – 167DL → GQ in isoform 2. 1 Publication2
Alternative sequenceiVSP_055786168 – 555Missing in isoform 2. 1 PublicationAdd BLAST388

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB078619 mRNA Translation: BAB84022.1
AK122960 mRNA Translation: BAG53821.1
AC008411 Genomic DNA No translation available.
AC010623 Genomic DNA No translation available.
CH471084 Genomic DNA Translation: EAW95875.1
BC089437 mRNA Translation: AAH89437.1
BC075010 mRNA Translation: AAH75010.1
BC075011 mRNA Translation: AAH75011.1
CCDSiCCDS4055.1 [Q8WYK0-1]
RefSeqiNP_570123.1, NM_130767.2 [Q8WYK0-1]
UniGeneiHs.591756

Genome annotation databases

EnsembliENST00000307624; ENSP00000303246; ENSG00000172497 [Q8WYK0-1]
ENST00000513751; ENSP00000421628; ENSG00000172497 [Q8WYK0-2]
GeneIDi134526
KEGGihsa:134526
UCSCiuc003khl.5 human [Q8WYK0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB078619 mRNA Translation: BAB84022.1
AK122960 mRNA Translation: BAG53821.1
AC008411 Genomic DNA No translation available.
AC010623 Genomic DNA No translation available.
CH471084 Genomic DNA Translation: EAW95875.1
BC089437 mRNA Translation: AAH89437.1
BC075010 mRNA Translation: AAH75010.1
BC075011 mRNA Translation: AAH75011.1
CCDSiCCDS4055.1 [Q8WYK0-1]
RefSeqiNP_570123.1, NM_130767.2 [Q8WYK0-1]
UniGeneiHs.591756

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3B7KX-ray2.70A/B/C7-316[»]
4MOBX-ray2.40A7-336[»]
4MOCX-ray2.50A7-336[»]
ProteinModelPortaliQ8WYK0
SMRiQ8WYK0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126405, 6 interactors
IntActiQ8WYK0, 9 interactors
STRINGi9606.ENSP00000303246

Chemistry databases

SwissLipidsiSLP:000001187

Protein family/group databases

TCDBi4.C.3.1.2 the acyl-coa thioesterase (acot) family

PTM databases

iPTMnetiQ8WYK0
PhosphoSitePlusiQ8WYK0

Polymorphism and mutation databases

BioMutaiACOT12
DMDMi25008183

Proteomic databases

PaxDbiQ8WYK0
PeptideAtlasiQ8WYK0
PRIDEiQ8WYK0
ProteomicsDBi75162

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000307624; ENSP00000303246; ENSG00000172497 [Q8WYK0-1]
ENST00000513751; ENSP00000421628; ENSG00000172497 [Q8WYK0-2]
GeneIDi134526
KEGGihsa:134526
UCSCiuc003khl.5 human [Q8WYK0-1]

Organism-specific databases

CTDi134526
DisGeNETi134526
EuPathDBiHostDB:ENSG00000172497.8
GeneCardsiACOT12
HGNCiHGNC:24436 ACOT12
HPAiHPA037723
HPA037724
MIMi614315 gene
neXtProtiNX_Q8WYK0
OpenTargetsiENSG00000172497
PharmGKBiPA142672657
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2763 Eukaryota
COG1607 LUCA
GeneTreeiENSGT00760000119297
HOGENOMiHOG000232032
HOVERGENiHBG023847
InParanoidiQ8WYK0
KOiK01067
OMAiRPLWDPH
OrthoDBiEOG091G06LZ
PhylomeDBiQ8WYK0
TreeFamiTF328368

Enzyme and pathway databases

UniPathwayi
UPA00231

BRENDAi3.1.2.1 2681
ReactomeiR-HSA-77289 Mitochondrial Fatty Acid Beta-Oxidation

Miscellaneous databases

EvolutionaryTraceiQ8WYK0
GeneWikiiACOT12
GenomeRNAii134526
PROiPR:Q8WYK0
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000172497 Expressed in 49 organ(s), highest expression level in right lobe of liver
CleanExiHS_ACOT12
GenevisibleiQ8WYK0 HS

Family and domain databases

Gene3Di3.30.530.20, 1 hit
InterProiView protein in InterPro
IPR033120 HOTDOG_ACOT
IPR029069 HotDog_dom_sf
IPR023393 START-like_dom_sf
IPR002913 START_lipid-bd_dom
IPR006683 Thioestr_dom
PfamiView protein in Pfam
PF03061 4HBT, 2 hits
PF01852 START, 1 hit
SUPFAMiSSF54637 SSF54637, 2 hits
PROSITEiView protein in PROSITE
PS51770 HOTDOG_ACOT, 2 hits
PS50848 START, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACO12_HUMAN
AccessioniPrimary (citable) accession number: Q8WYK0
Secondary accession number(s): B3KVK9, Q5FWE9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: March 1, 2002
Last modified: October 10, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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