UniProtKB - Q8WY41 (NANO1_HUMAN)
Protein
Nanos homolog 1
Gene
NANOS1
Organism
Homo sapiens (Human)
Status
Functioni
May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion.2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 213 – 267 | Nanos-typePROSITE-ProRule annotationAdd BLAST | 55 |
GO - Molecular functioni
- mRNA binding Source: GO_Central
- translation repressor activity Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- cell migration Source: UniProtKB
- cerebellar neuron development Source: Ensembl
- epithelial cell migration Source: UniProtKB
- negative regulation of translation Source: BHF-UCL
- oogenesis Source: GO_Central
- positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: BHF-UCL
- posttranscriptional regulation of gene expression Source: MGI
- regulation of cell growth Source: Ensembl
- tissue homeostasis Source: Ensembl
Keywordsi
Molecular function | Repressor, RNA-binding |
Biological process | Translation regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q8WY41 |
Names & Taxonomyi
Protein namesi | Recommended name: Nanos homolog 1Short name: NOS-1 Alternative name(s): EC_Rep1a |
Gene namesi | Name:NANOS1 Synonyms:NOS1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000188613.6 |
HGNCi | HGNC:23044, NANOS1 |
MIMi | 608226, gene |
neXtProti | NX_Q8WY41 |
Subcellular locationi
Other locations
- perinuclear region 3 Publications
- Cytoplasm 3 Publications
Note: Colocalizes with SNAPIN and PUM2 in the perinuclear region of germ cells.2 Publications
Other locations
- cytoplasm Source: UniProtKB
- perinuclear region of cytoplasm Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Involvement in diseasei
Spermatogenic failure 12 (SPGF12)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn infertility disorder caused by spermatogenesis defects. It results in decreased sperm motility, concentration, and multiple sperm structural defects. Non-obstructive azoospermia, oligozoospermia and oligo-astheno-teratozoospermia are features observed in SPGF12 patients.
Related information in OMIMFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_070570 | 78 | Missing in SPGF12. 1 Publication | 1 | |
Natural variantiVAR_070571 | 173 | Missing in SPGF12. 1 Publication | 1 |
Keywords - Diseasei
Disease mutationOrganism-specific databases
DisGeNETi | 340719 |
MalaCardsi | NANOS1 |
MIMi | 615413, phenotype |
OpenTargetsi | ENSG00000188613 |
Orphaneti | 399805, Male infertility with azoospermia or oligozoospermia due to single gene mutation 399808, Male infertility with teratozoospermia due to single gene mutation |
PharmGKBi | PA134876543 |
Miscellaneous databases
Pharosi | Q8WY41, Tbio |
Polymorphism and mutation databases
BioMutai | NANOS1 |
DMDMi | 41688589 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207685 | 1 – 292 | Nanos homolog 1Add BLAST | 292 |
Proteomic databases
MassIVEi | Q8WY41 |
PaxDbi | Q8WY41 |
PeptideAtlasi | Q8WY41 |
PRIDEi | Q8WY41 |
ProteomicsDBi | 75126 |
PTM databases
iPTMneti | Q8WY41 |
PhosphoSitePlusi | Q8WY41 |
Expressioni
Tissue specificityi
Testis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness.5 Publications
Developmental stagei
Fetal ovary and fetal testis (at protein level).1 Publication
Inductioni
Down-regulated by E-cadherin.1 Publication
Gene expression databases
Bgeei | ENSG00000188613, Expressed in secondary oocyte and 173 other tissues |
ExpressionAtlasi | Q8WY41, baseline and differential |
Genevisiblei | Q8WY41, HS |
Organism-specific databases
HPAi | ENSG00000188613, Tissue enhanced (skeletal) |
Interactioni
Subunit structurei
Interacts with PUM2, SNAPIN and CTNNB1.
Interacts (via N-terminal region) with CTNND1.
Interacts with DDX20 (via N-terminal region).
4 PublicationsBinary interactionsi
Q8WY41
With | #Exp. | IntAct |
---|---|---|
CTNND1 - isoform 3A [O60716-21] | 2 | EBI-9630165,EBI-9634525 |
Protein-protein interaction databases
BioGRIDi | 131092, 4 interactors |
ELMi | Q8WY41 |
IntActi | Q8WY41, 1 interactor |
STRINGi | 9606.ENSP00000393275 |
Miscellaneous databases
RNActi | Q8WY41, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8WY41 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 40 – 56 | Essential for its translational repressor activityBy similarityAdd BLAST | 17 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 214 – 241 | C2HC 1Add BLAST | 28 | |
Motifi | 249 – 265 | C2HC 2Add BLAST | 17 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 136 – 204 | Ala-richAdd BLAST | 69 |
Domaini
The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.PROSITE-ProRule annotation
The N-terminal region and C-terminal zinc-finger RNA-binding domain are both necessary for interaction with SNAPIN.
Sequence similaritiesi
Belongs to the nanos family.PROSITE-ProRule annotation
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 213 – 267 | Nanos-typePROSITE-ProRule annotationAdd BLAST | 55 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG4602, Eukaryota |
GeneTreei | ENSGT00950000183135 |
HOGENOMi | CLU_094055_0_0_1 |
InParanoidi | Q8WY41 |
OMAi | CCSPHAG |
OrthoDBi | 1633105at2759 |
PhylomeDBi | Q8WY41 |
TreeFami | TF326882 |
Family and domain databases
Gene3Di | 1.10.150.680, 1 hit |
IDEALi | IID00517 |
InterProi | View protein in InterPro IPR008705, Nanos/Xcar2 IPR038129, Nanos_sf IPR024161, Znf_nanos-typ |
PANTHERi | PTHR12887, PTHR12887, 1 hit |
Pfami | View protein in Pfam PF05741, zf-nanos, 1 hit |
PROSITEi | View protein in PROSITE PS51522, ZF_NANOS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8WY41-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEAFPWAPRS PRRGRAPPPM ALVPSARYVS APGPAHPQPF SSWNDYLGLA
60 70 80 90 100
TLITKAVDGE PRFGCARGGN GGGGSPPSSS SSSCCSPHTG AGPGALGPAL
110 120 130 140 150
GPPDYDEDDD DDSDEPGSRG RYLGSALELR ALELCAGPAE AGLLEERFAE
160 170 180 190 200
LSPFAGRAAA VLLGCAPAAA AAATTTSEAT PREERAPAWA AEPRLHAASG
210 220 230 240 250
AAAARLLKPE LQVCVFCRNN KEAMALYTTH ILKGPDGRVL CPVLRRYTCP
260 270 280 290
LCGASGDNAH TIKYCPLSKV PPPPARPPPR SARDGPPGKK LR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ5T9H5 | Q5T9H5_HUMAN | Nanos homolog 1 | NANOS1 | 84 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_070569 | 34 | P → T1 PublicationCorresponds to variant dbSNP:rs191267549Ensembl. | 1 | |
Natural variantiVAR_070570 | 78 | Missing in SPGF12. 1 Publication | 1 | |
Natural variantiVAR_070571 | 173 | Missing in SPGF12. 1 Publication | 1 | |
Natural variantiVAR_070572 | 246 | R → H Found in a patient affected by oligo-astheno-teratozoospermia also carrying Y-276 on the same allele; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs587777767EnsemblClinVar. | 1 | |
Natural variantiVAR_070573 | 276 | R → Y Found in a patient affected by oligo-astheno-teratozoospermia also carrying H-246 on the same allele; requires 2 nucleotide substitutions; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs587777768EnsemblClinVar. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF275269 mRNA Translation: AAL36982.2 AF458985 mRNA Translation: AAQ04765.1 AL157788 Genomic DNA No translation available. |
CCDSi | CCDS7607.1 |
RefSeqi | NP_955631.1, NM_199461.3 |
Genome annotation databases
Ensembli | ENST00000425699; ENSP00000393275; ENSG00000188613 |
GeneIDi | 340719 |
KEGGi | hsa:340719 |
UCSCi | uc009xzf.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF275269 mRNA Translation: AAL36982.2 AF458985 mRNA Translation: AAQ04765.1 AL157788 Genomic DNA No translation available. |
CCDSi | CCDS7607.1 |
RefSeqi | NP_955631.1, NM_199461.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4CQO | X-ray | 2.80 | B/D | 40-56 | [»] | |
SMRi | Q8WY41 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 131092, 4 interactors |
ELMi | Q8WY41 |
IntActi | Q8WY41, 1 interactor |
STRINGi | 9606.ENSP00000393275 |
PTM databases
iPTMneti | Q8WY41 |
PhosphoSitePlusi | Q8WY41 |
Polymorphism and mutation databases
BioMutai | NANOS1 |
DMDMi | 41688589 |
Proteomic databases
MassIVEi | Q8WY41 |
PaxDbi | Q8WY41 |
PeptideAtlasi | Q8WY41 |
PRIDEi | Q8WY41 |
ProteomicsDBi | 75126 |
Protocols and materials databases
Antibodypediai | 32079, 291 antibodies |
DNASUi | 340719 |
Genome annotation databases
Ensembli | ENST00000425699; ENSP00000393275; ENSG00000188613 |
GeneIDi | 340719 |
KEGGi | hsa:340719 |
UCSCi | uc009xzf.2, human |
Organism-specific databases
CTDi | 340719 |
DisGeNETi | 340719 |
EuPathDBi | HostDB:ENSG00000188613.6 |
GeneCardsi | NANOS1 |
HGNCi | HGNC:23044, NANOS1 |
HPAi | ENSG00000188613, Tissue enhanced (skeletal) |
MalaCardsi | NANOS1 |
MIMi | 608226, gene 615413, phenotype |
neXtProti | NX_Q8WY41 |
OpenTargetsi | ENSG00000188613 |
Orphaneti | 399805, Male infertility with azoospermia or oligozoospermia due to single gene mutation 399808, Male infertility with teratozoospermia due to single gene mutation |
PharmGKBi | PA134876543 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4602, Eukaryota |
GeneTreei | ENSGT00950000183135 |
HOGENOMi | CLU_094055_0_0_1 |
InParanoidi | Q8WY41 |
OMAi | CCSPHAG |
OrthoDBi | 1633105at2759 |
PhylomeDBi | Q8WY41 |
TreeFami | TF326882 |
Enzyme and pathway databases
PathwayCommonsi | Q8WY41 |
Miscellaneous databases
BioGRID-ORCSi | 340719, 12 hits in 838 CRISPR screens |
ChiTaRSi | NANOS1, human |
GenomeRNAii | 340719 |
Pharosi | Q8WY41, Tbio |
PROi | PR:Q8WY41 |
RNActi | Q8WY41, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000188613, Expressed in secondary oocyte and 173 other tissues |
ExpressionAtlasi | Q8WY41, baseline and differential |
Genevisiblei | Q8WY41, HS |
Family and domain databases
Gene3Di | 1.10.150.680, 1 hit |
IDEALi | IID00517 |
InterProi | View protein in InterPro IPR008705, Nanos/Xcar2 IPR038129, Nanos_sf IPR024161, Znf_nanos-typ |
PANTHERi | PTHR12887, PTHR12887, 1 hit |
Pfami | View protein in Pfam PF05741, zf-nanos, 1 hit |
PROSITEi | View protein in PROSITE PS51522, ZF_NANOS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NANO1_HUMAN | |
Accessioni | Q8WY41Primary (citable) accession number: Q8WY41 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 2, 2004 |
Last sequence update: | February 2, 2004 | |
Last modified: | December 2, 2020 | |
This is version 142 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations