UniProtKB - Q8WXS8 (ATS14_HUMAN)
Protein
A disintegrin and metalloproteinase with thrombospondin motifs 14
Gene
ADAMTS14
Organism
Homo sapiens (Human)
Status
Functioni
Has aminoprocollagen type I processing activity in the absence of ADAMTS2 (PubMed:11741898). Seems to be synthesized as a latent enzyme that requires activation to display aminoprocollagen peptidase activity (PubMed:11741898). Cleaves lysyl oxidase LOX at a site downstream of its propeptide cleavage site to produce a short LOX form (PubMed:31152061).2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 398 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Active sitei | 399 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 402 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 408 | Zinc; catalyticPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- metalloendopeptidase activity Source: GO_Central
GO - Biological processi
- collagen catabolic process Source: UniProtKB-KW
- collagen fibril organization Source: Ensembl
- extracellular matrix organization Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Biological process | Collagen degradation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q8WXS8 |
Reactomei | R-HSA-1650814, Collagen biosynthesis and modifying enzymes R-HSA-5083635, Defective B3GALTL causes Peters-plus syndrome (PpS) R-HSA-5173214, O-glycosylation of TSR domain-containing proteins |
Protein family/group databases
MEROPSi | M12.024 |
Names & Taxonomyi
Protein namesi | Recommended name: A disintegrin and metalloproteinase with thrombospondin motifs 14 (EC:3.4.24.-)Short name: ADAM-TS 14 Short name: ADAM-TS14 Short name: ADAMTS-14 |
Gene namesi | Name:ADAMTS14 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:14899, ADAMTS14 |
MIMi | 607506, gene |
neXtProti | NX_Q8WXS8 |
VEuPathDBi | HostDB:ENSG00000138316.10 |
Subcellular locationi
Extracellular region or secreted
- extracellular matrix By similarity
Extracellular region or secreted
- extracellular matrix Source: GO_Central
- extracellular region Source: Reactome
Keywords - Cellular componenti
Extracellular matrix, SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 140766 |
OpenTargetsi | ENSG00000138316 |
PharmGKBi | PA24540 |
Miscellaneous databases
Pharosi | Q8WXS8, Tbio |
Genetic variation databases
BioMutai | ADAMTS14 |
DMDMi | 317373325 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
PropeptideiPRO_0000029190 | 23 – 252 | By similarityAdd BLAST | 230 | |
ChainiPRO_0000029191 | 253 – 1223 | A disintegrin and metalloproteinase with thrombospondin motifs 14Add BLAST | 971 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 109 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 336 ↔ 382 | By similarity | ||
Disulfide bondi | 376 ↔ 455 | By similarity | ||
Disulfide bondi | 415 ↔ 441 | By similarity | ||
Glycosylationi | 475 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 482 ↔ 507 | By similarity | ||
Disulfide bondi | 493 ↔ 516 | By similarity | ||
Disulfide bondi | 502 ↔ 535 | By similarity | ||
Disulfide bondi | 529 ↔ 540 | By similarity | ||
Disulfide bondi | 564 ↔ 601 | By similarity | ||
Disulfide bondi | 568 ↔ 606 | By similarity | ||
Disulfide bondi | 579 ↔ 591 | By similarity | ||
Glycosylationi | 941 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 980 ↔ 1016 | By similarity | ||
Disulfide bondi | 984 ↔ 1021 | By similarity | ||
Disulfide bondi | 995 ↔ 1005 | By similarity | ||
Glycosylationi | 1027 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
The precursor is cleaved by a furin endopeptidase.By similarity
Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X2-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).By similarity
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, ZymogenProteomic databases
MassIVEi | Q8WXS8 |
PaxDbi | Q8WXS8 |
PeptideAtlasi | Q8WXS8 |
PRIDEi | Q8WXS8 |
ProteomicsDBi | 75096 [Q8WXS8-1] 75097 [Q8WXS8-2] 75098 [Q8WXS8-3] 75099 [Q8WXS8-4] |
PTM databases
GlyGeni | Q8WXS8, 4 sites |
iPTMneti | Q8WXS8 |
PhosphoSitePlusi | Q8WXS8 |
Expressioni
Tissue specificityi
Expressed in retina and at low levels in brain, lung and placenta (PubMed:11779638). High expression in fetal tissues (PubMed:11867212).2 Publications
Gene expression databases
Bgeei | ENSG00000138316, Expressed in stomach and 149 other tissues |
Genevisiblei | Q8WXS8, HS |
Organism-specific databases
HPAi | ENSG00000138316, Tissue enhanced (brain, gallbladder, placenta) |
Interactioni
Protein-protein interaction databases
IntActi | Q8WXS8, 1 interactor |
STRINGi | 9606.ENSP00000362304 |
Miscellaneous databases
RNActi | Q8WXS8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 259 – 460 | Peptidase M12BPROSITE-ProRule annotationAdd BLAST | 202 | |
Domaini | 461 – 551 | DisintegrinAdd BLAST | 91 | |
Domaini | 552 – 607 | TSP type-1 1PROSITE-ProRule annotationAdd BLAST | 56 | |
Domaini | 847 – 907 | TSP type-1 2PROSITE-ProRule annotationAdd BLAST | 61 | |
Domaini | 908 – 967 | TSP type-1 3PROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 968 – 1022 | TSP type-1 4PROSITE-ProRule annotationAdd BLAST | 55 | |
Domaini | 1059 – 1097 | PLACPROSITE-ProRule annotationAdd BLAST | 39 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 730 – 846 | SpacerAdd BLAST | 117 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 608 – 729 | Cys-richAdd BLAST | 122 | |
Compositional biasi | 875 – 878 | Poly-Arg | 4 | |
Compositional biasi | 1100 – 1221 | Pro-richAdd BLAST | 122 |
Domaini
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.By similarity
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | KOG3538, Eukaryota |
GeneTreei | ENSGT00940000158426 |
HOGENOMi | CLU_000660_4_1_1 |
InParanoidi | Q8WXS8 |
OMAi | HSWIPYE |
OrthoDBi | 79609at2759 |
PhylomeDBi | Q8WXS8 |
TreeFami | TF313537 |
Family and domain databases
Gene3Di | 2.20.100.10, 4 hits 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR041645, ADAM_CR_2 IPR006586, ADAM_Cys-rich IPR010294, ADAM_spacer1 IPR013273, ADAMTS/ADAMTS-like IPR024079, MetalloPept_cat_dom_sf IPR001590, Peptidase_M12B IPR002870, Peptidase_M12B_N IPR010909, PLAC IPR000884, TSP1_rpt IPR036383, TSP1_rpt_sf |
Pfami | View protein in Pfam PF17771, ADAM_CR_2, 1 hit PF05986, ADAM_spacer1, 1 hit PF01562, Pep_M12B_propep, 1 hit PF01421, Reprolysin, 1 hit PF00090, TSP_1, 1 hit |
PRINTSi | PR01857, ADAMTSFAMILY |
SMARTi | View protein in SMART SM00608, ACR, 1 hit SM00209, TSP1, 4 hits |
SUPFAMi | SSF82895, SSF82895, 4 hits |
PROSITEi | View protein in PROSITE PS50215, ADAM_MEPRO, 1 hit PS50900, PLAC, 1 hit PS50092, TSP1, 4 hits |
s (4)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative promoter usage and alternative splicing. AlignAdd to basketIsoform A (identifier: Q8WXS8-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAPLRALLSY LLPLHCALCA AAGSRTPELH LSGKLSDYGV TVPCSTDFRG
60 70 80 90 100
RFLSHVVSGP AAASAGSMVV DTPPTLPRHS SHLRVARSPL HPGGTLWPGR
110 120 130 140 150
VGRHSLYFNV TVFGKELHLR LRPNRRLVVP GSSVEWQEDF RELFRQPLRQ
160 170 180 190 200
ECVYTGGVTG MPGAAVAISN CDGLAGLIRT DSTDFFIEPL ERGQQEKEAS
210 220 230 240 250
GRTHVVYRRE AVQQEWAEPD GDLHNEAFGL GDLPNLLGLV GDQLGDTERK
260 270 280 290 300
RRHAKPGSYS IEVLLVVDDS VVRFHGKEHV QNYVLTLMNI VDEIYHDESL
310 320 330 340 350
GVHINIALVR LIMVGYRQSL SLIERGNPSR SLEQVCRWAH SQQRQDPSHA
360 370 380 390 400
EHHDHVVFLT RQDFGPSGYA PVTGMCHPLR SCALNHEDGF SSAFVIAHET
410 420 430 440 450
GHVLGMEHDG QGNGCADETS LGSVMAPLVQ AAFHRFHWSR CSKLELSRYL
460 470 480 490 500
PSYDCLLDDP FDPAWPQPPE LPGINYSMDE QCRFDFGSGY QTCLAFRTFE
510 520 530 540 550
PCKQLWCSHP DNPYFCKTKK GPPLDGTECA PGKWCFKGHC IWKSPEQTYG
560 570 580 590 600
QDGGWSSWTK FGSCSRSCGG GVRSRSRSCN NPSPAYGGRL CLGPMFEYQV
610 620 630 640 650
CNSEECPGTY EDFRAQQCAK RNSYYVHQNA KHSWVPYEPD DDAQKCELIC
660 670 680 690 700
QSADTGDVVF MNQVVHDGTR CSYRDPYSVC ARGECVPVGC DKEVGSMKAD
710 720 730 740 750
DKCGVCGGDN SHCRTVKGTL GKASKQAGAL KLVQIPAGAR HIQIEALEKS
760 770 780 790 800
PHRIVVKNQV TGSFILNPKG KEATSRTFTA MGLEWEDAVE DAKESLKTSG
810 820 830 840 850
PLPEAIAILA LPPTEGGPRS SLAYKYVIHE DLLPLIGSNN VLLEEMDTYE
860 870 880 890 900
WALKSWAPCS KACGGGIQFT KYGCRRRRDH HMVQRHLCDH KKRPKPIRRR
910 920 930 940 950
CNQHPCSQPV WVTEEWGACS RSCGKLGVQT RGIQCLLPLS NGTHKVMPAK
960 970 980 990 1000
ACAGDRPEAR RPCLRVPCPA QWRLGAWSQC SATCGEGIQQ RQVVCRTNAN
1010 1020 1030 1040 1050
SLGHCEGDRP DTVQVCSLPA CGGNHQNSTV RADVWELGTP EGQWVPQSEP
1060 1070 1080 1090 1100
LHPINKISST EPCTGDRSVF CQMEVLDRYC SIPGYHRLCC VSCIKKASGP
1110 1120 1130 1140 1150
NPGPDPGPTS LPPFSTPGSP LPGPQDPADA AEPPGKPTGS EDHQHGRATQ
1160 1170 1180 1190 1200
LPGALDTSSP GTQHPFAPET PIPGASWSIS PTTPGGLPWG WTQTPTPVPE
1210 1220
DKGQPGEDLR HPGTSLPAAS PVT
Note: Produced by alternative promoter usage.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 868 | Q → R in CAC87943 (PubMed:11867212).Curated | 1 | |
Sequence conflicti | 884 | Q → H in CAC87943 (PubMed:11867212).Curated | 1 | |
Sequence conflicti | 901 | C → S in CAC87943 (PubMed:11867212).Curated | 1 | |
Sequence conflicti | 923 | C → Y in CAC87943 (PubMed:11867212).Curated | 1 | |
Sequence conflicti | 1024 | N → S in CAC87943 (PubMed:11867212).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_047837 | 179 | R → C. Corresponds to variant dbSNP:rs34022601Ensembl. | 1 | |
Natural variantiVAR_047838 | 590 | L → P3 PublicationsCorresponds to variant dbSNP:rs10823607Ensembl. | 1 | |
Natural variantiVAR_047839 | 937 | L → M. Corresponds to variant dbSNP:rs12774070Ensembl. | 1 | |
Natural variantiVAR_047840 | 1017 | S → N. Corresponds to variant dbSNP:rs10999516Ensembl. | 1 | |
Natural variantiVAR_047841 | 1049 | E → G. Corresponds to variant dbSNP:rs4747096Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_006958 | 1 – 67 | Missing in isoform B and isoform C. 1 PublicationAdd BLAST | 67 | |
Alternative sequenceiVSP_005501 | 368 | G → GMQG in isoform C and isoform D. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF358666 mRNA Translation: AAL40229.1 AJ345098 mRNA Translation: CAC87943.1 AL355344 Genomic DNA No translation available. AL358817 Genomic DNA No translation available. CH471083 Genomic DNA Translation: EAW54409.1 CH471083 Genomic DNA Translation: EAW54410.1 AF366351 mRNA Translation: AAL79814.1 |
CCDSi | CCDS7306.1 [Q8WXS8-1] CCDS7307.1 [Q8WXS8-4] |
RefSeqi | NP_542453.2, NM_080722.4 [Q8WXS8-1] NP_631894.2, NM_139155.3 [Q8WXS8-4] |
Genome annotation databases
Ensembli | ENST00000373207; ENSP00000362303; ENSG00000138316 [Q8WXS8-1] ENST00000373208; ENSP00000362304; ENSG00000138316 [Q8WXS8-4] |
GeneIDi | 140766 |
KEGGi | hsa:140766 |
UCSCi | uc001jrg.4, human [Q8WXS8-1] |
Keywords - Coding sequence diversityi
Alternative promoter usage, Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF358666 mRNA Translation: AAL40229.1 AJ345098 mRNA Translation: CAC87943.1 AL355344 Genomic DNA No translation available. AL358817 Genomic DNA No translation available. CH471083 Genomic DNA Translation: EAW54409.1 CH471083 Genomic DNA Translation: EAW54410.1 AF366351 mRNA Translation: AAL79814.1 |
CCDSi | CCDS7306.1 [Q8WXS8-1] CCDS7307.1 [Q8WXS8-4] |
RefSeqi | NP_542453.2, NM_080722.4 [Q8WXS8-1] NP_631894.2, NM_139155.3 [Q8WXS8-4] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | Q8WXS8, 1 interactor |
STRINGi | 9606.ENSP00000362304 |
Protein family/group databases
MEROPSi | M12.024 |
PTM databases
GlyGeni | Q8WXS8, 4 sites |
iPTMneti | Q8WXS8 |
PhosphoSitePlusi | Q8WXS8 |
Genetic variation databases
BioMutai | ADAMTS14 |
DMDMi | 317373325 |
Proteomic databases
MassIVEi | Q8WXS8 |
PaxDbi | Q8WXS8 |
PeptideAtlasi | Q8WXS8 |
PRIDEi | Q8WXS8 |
ProteomicsDBi | 75096 [Q8WXS8-1] 75097 [Q8WXS8-2] 75098 [Q8WXS8-3] 75099 [Q8WXS8-4] |
Protocols and materials databases
Antibodypediai | 29052, 37 antibodies |
DNASUi | 140766 |
Genome annotation databases
Ensembli | ENST00000373207; ENSP00000362303; ENSG00000138316 [Q8WXS8-1] ENST00000373208; ENSP00000362304; ENSG00000138316 [Q8WXS8-4] |
GeneIDi | 140766 |
KEGGi | hsa:140766 |
UCSCi | uc001jrg.4, human [Q8WXS8-1] |
Organism-specific databases
CTDi | 140766 |
DisGeNETi | 140766 |
GeneCardsi | ADAMTS14 |
HGNCi | HGNC:14899, ADAMTS14 |
HPAi | ENSG00000138316, Tissue enhanced (brain, gallbladder, placenta) |
MIMi | 607506, gene |
neXtProti | NX_Q8WXS8 |
OpenTargetsi | ENSG00000138316 |
PharmGKBi | PA24540 |
VEuPathDBi | HostDB:ENSG00000138316.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3538, Eukaryota |
GeneTreei | ENSGT00940000158426 |
HOGENOMi | CLU_000660_4_1_1 |
InParanoidi | Q8WXS8 |
OMAi | HSWIPYE |
OrthoDBi | 79609at2759 |
PhylomeDBi | Q8WXS8 |
TreeFami | TF313537 |
Enzyme and pathway databases
PathwayCommonsi | Q8WXS8 |
Reactomei | R-HSA-1650814, Collagen biosynthesis and modifying enzymes R-HSA-5083635, Defective B3GALTL causes Peters-plus syndrome (PpS) R-HSA-5173214, O-glycosylation of TSR domain-containing proteins |
Miscellaneous databases
BioGRID-ORCSi | 140766, 4 hits in 876 CRISPR screens |
ChiTaRSi | ADAMTS14, human |
GenomeRNAii | 140766 |
Pharosi | Q8WXS8, Tbio |
PROi | PR:Q8WXS8 |
RNActi | Q8WXS8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000138316, Expressed in stomach and 149 other tissues |
Genevisiblei | Q8WXS8, HS |
Family and domain databases
Gene3Di | 2.20.100.10, 4 hits 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR041645, ADAM_CR_2 IPR006586, ADAM_Cys-rich IPR010294, ADAM_spacer1 IPR013273, ADAMTS/ADAMTS-like IPR024079, MetalloPept_cat_dom_sf IPR001590, Peptidase_M12B IPR002870, Peptidase_M12B_N IPR010909, PLAC IPR000884, TSP1_rpt IPR036383, TSP1_rpt_sf |
Pfami | View protein in Pfam PF17771, ADAM_CR_2, 1 hit PF05986, ADAM_spacer1, 1 hit PF01562, Pep_M12B_propep, 1 hit PF01421, Reprolysin, 1 hit PF00090, TSP_1, 1 hit |
PRINTSi | PR01857, ADAMTSFAMILY |
SMARTi | View protein in SMART SM00608, ACR, 1 hit SM00209, TSP1, 4 hits |
SUPFAMi | SSF82895, SSF82895, 4 hits |
PROSITEi | View protein in PROSITE PS50215, ADAM_MEPRO, 1 hit PS50900, PLAC, 1 hit PS50092, TSP1, 4 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ATS14_HUMAN | |
Accessioni | Q8WXS8Primary (citable) accession number: Q8WXS8 Secondary accession number(s): Q5T4G0 Q8TEY8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 28, 2003 |
Last sequence update: | January 11, 2011 | |
Last modified: | February 10, 2021 | |
This is version 170 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot