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Protein

Paraspeckle component 1

Gene

PSPC1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Binds to poly(A), poly(G) and poly(U) RNA homopolymers. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer (By similarity). Together with NONO, required for the formation of nuclear paraspeckles. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Repressor, RNA-binding
Biological processBiological rhythms, Immunity, Innate immunity, Transcription, Transcription regulation

Enzyme and pathway databases

SIGNORiQ8WXF1

Names & Taxonomyi

Protein namesi
Recommended name:
Paraspeckle component 1
Alternative name(s):
Paraspeckle protein 1
Gene namesi
Name:PSPC1
Synonyms:PSP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

EuPathDBiHostDB:ENSG00000121390.18
HGNCiHGNC:20320 PSPC1
MIMi612408 gene
neXtProtiNX_Q8WXF1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi119F → A: Abolishes accumulation in paraspeckles, but not in perinucleolar caps; when associated with A-121; A-198 and A-200. 1 Publication1
Mutagenesisi121F → A: Abolishes accumulation in paraspeckles, but not in perinucleolar caps; when associated with A-119; A-198 and A-200. 1 Publication1
Mutagenesisi198K → A: Abolishes accumulation in paraspeckles, but not in perinucleolar caps; when associated with A-119; A-121 and A-200. 1 Publication1
Mutagenesisi200F → A: Abolishes accumulation in paraspeckles, but not in perinucleolar caps; when associated with A-119; A-121 and A-198. 1 Publication1
Mutagenesisi275Y → A: Abolishes interaction with NONO and localization in nuclear paraspeckles; when associated with A-279. 1 Publication1
Mutagenesisi279W → A: Abolishes interaction with NONO and localization in nuclear paraspeckles; when associated with A-275. 1 Publication1

Organism-specific databases

DisGeNETi55269
OpenTargetsiENSG00000121390
PharmGKBiPA134968603

Polymorphism and mutation databases

BioMutaiPSPC1
DMDMi74762636

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002975401 – 523Paraspeckle component 1Add BLAST523

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei409PhosphoserineCombined sources1
Modified residuei473PhosphoserineCombined sources1
Modified residuei477PhosphoserineCombined sources1
Modified residuei507Omega-N-methylarginineCombined sources1
Modified residuei509PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ8WXF1
MaxQBiQ8WXF1
PaxDbiQ8WXF1
PeptideAtlasiQ8WXF1
PRIDEiQ8WXF1
ProteomicsDBi75023
75024 [Q8WXF1-2]

PTM databases

iPTMnetiQ8WXF1
PhosphoSitePlusiQ8WXF1
SwissPalmiQ8WXF1

Expressioni

Tissue specificityi

Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.1 Publication

Gene expression databases

BgeeiENSG00000121390 Expressed in 216 organ(s), highest expression level in testis
CleanExiHS_PSPC1
ExpressionAtlasiQ8WXF1 baseline and differential
GenevisibleiQ8WXF1 HS

Organism-specific databases

HPAiHPA038904

Interactioni

Subunit structurei

Forms heterodimers with NONO; this involves formation of a coiled coil domain by helices from both proteins. Found in a RNP complex with CAT2 transcribed nuclear RNA (CTN-RNA). Interaction with NONO is required for its targeting to paraspeckles and perinucleolar caps. Interacts with SFPQ (By similarity). Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 RNA.By similarity2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi120558, 47 interactors
DIPiDIP-39028N
IntActiQ8WXF1, 27 interactors
MINTiQ8WXF1
STRINGi9606.ENSP00000343966

Structurei

Secondary structure

1523
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8WXF1
SMRiQ8WXF1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 154RRM 1PROSITE-ProRule annotationAdd BLAST73
Domaini156 – 237RRM 2PROSITE-ProRule annotationAdd BLAST82

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 358Sufficient for paraspeckles localizationAdd BLAST234
Regioni231 – 358Sufficient for perinucleolar caps localization and interaction with NONO1 PublicationAdd BLAST128

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili283 – 377Sequence analysisAdd BLAST95

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi394 – 516Gly-richAdd BLAST123

Sequence similaritiesi

Belongs to the PSPC family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0115 Eukaryota
ENOG410XQA0 LUCA
GeneTreeiENSGT00390000005004
HOGENOMiHOG000231095
HOVERGENiHBG009801
InParanoidiQ8WXF1
OMAiMDNREQE
OrthoDBiEOG091G09T7
PhylomeDBiQ8WXF1
TreeFamiTF315795

Family and domain databases

CDDicd12586 RRM1_PSP1, 1 hit
cd12589 RRM2_PSP1, 1 hit
Gene3Di3.30.70.330, 2 hits
InterProiView protein in InterPro
IPR012975 NOPS
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR034522 PSP1_RRM1
IPR034523 PSP1_RRM2
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF08075 NOPS, 1 hit
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00360 RRM, 2 hits
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8WXF1-1) [UniParc]FASTAAdd to basket
Also known as: PSP1-alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MMLRGNLKQV RIEKNPARLR ALESAVGESE PAAAAAMALA LAGEPAPPAP
60 70 80 90 100
APPEDHPDEE MGFTIDIKSF LKPGEKTYTQ RCRLFVGNLP TDITEEDFKR
110 120 130 140 150
LFERYGEPSE VFINRDRGFG FIRLESRTLA EIAKAELDGT ILKSRPLRIR
160 170 180 190 200
FATHGAALTV KNLSPVVSNE LLEQAFSQFG PVEKAVVVVD DRGRATGKGF
210 220 230 240 250
VEFAAKPPAR KALERCGDGA FLLTTTPRPV IVEPMEQFDD EDGLPEKLMQ
260 270 280 290 300
KTQQYHKERE QPPRFAQPGT FEFEYASRWK ALDEMEKQQR EQVDRNIREA
310 320 330 340 350
KEKLEAEMEA ARHEHQLMLM RQDLMRRQEE LRRLEELRNQ ELQKRKQIQL
360 370 380 390 400
RHEEEHRRRE EEMIRHREQE ELRRQQEGFK PNYMENREQE MRMGDMGPRG
410 420 430 440 450
AINMGDAFSP APAGNQGPPP MMGMNMNNRA TIPGPPMGPG PAMGPEGAAN
460 470 480 490 500
MGTPMMPDNG AVHNDRFPQG PPSQMGSPMG SRTGSETPQA PMSGVGPVSG
510 520
GPGGFGRGSQ GGNFEGPNKR RRY
Length:523
Mass (Da):58,744
Last modified:March 1, 2002 - v1
Checksum:i3A8C44079D64BBF0
GO
Isoform 2 (identifier: Q8WXF1-2) [UniParc]FASTAAdd to basket
Also known as: PSP1-beta

The sequence of this isoform differs from the canonical sequence as follows:
     387-393: REQEMRM → GDKRKCG
     394-523: Missing.

Show »
Length:393
Mass (Da):45,571
Checksum:i67F1A7EBF93050B2
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
X6RDA4X6RDA4_HUMAN
Paraspeckle component 1
PSPC1
248Annotation score:
A0A0U1RQF9A0A0U1RQF9_HUMAN
Paraspeckle component 1
PSPC1
33Annotation score:

Sequence cautioni

The sequence BAA91924 differs from that shown. Reason: Erroneous termination at position 14. Translated as Lys.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_027274387 – 393REQEMRM → GDKRKCG in isoform 2. 5 Publications7
Alternative sequenceiVSP_027275394 – 523Missing in isoform 2. 5 PublicationsAdd BLAST130

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448795 mRNA Translation: AAL59601.1
AF449627 mRNA Translation: AAL59602.1
AK001817 mRNA Translation: BAA91924.1 Sequence problems.
AL354808 Genomic DNA No translation available.
CH471075 Genomic DNA Translation: EAX08232.1
BC014184 mRNA Translation: AAH14184.2
CR457272 mRNA Translation: CAG33553.1
AL834505 mRNA Translation: CAD39162.1
CCDSiCCDS41870.1 [Q8WXF1-1]
CCDS86343.1 [Q8WXF1-2]
RefSeqiNP_001035879.1, NM_001042414.2 [Q8WXF1-1]
XP_006719907.1, XM_006719844.3
XP_016876139.1, XM_017020650.1
UniGeneiHs.213198

Genome annotation databases

EnsembliENST00000338910; ENSP00000343966; ENSG00000121390 [Q8WXF1-1]
ENST00000471658; ENSP00000436038; ENSG00000121390 [Q8WXF1-2]
ENST00000492741; ENSP00000435921; ENSG00000121390 [Q8WXF1-2]
ENST00000619300; ENSP00000481916; ENSG00000121390 [Q8WXF1-1]
GeneIDi55269
KEGGihsa:55269
UCSCiuc021rgx.2 human [Q8WXF1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448795 mRNA Translation: AAL59601.1
AF449627 mRNA Translation: AAL59602.1
AK001817 mRNA Translation: BAA91924.1 Sequence problems.
AL354808 Genomic DNA No translation available.
CH471075 Genomic DNA Translation: EAX08232.1
BC014184 mRNA Translation: AAH14184.2
CR457272 mRNA Translation: CAG33553.1
AL834505 mRNA Translation: CAD39162.1
CCDSiCCDS41870.1 [Q8WXF1-1]
CCDS86343.1 [Q8WXF1-2]
RefSeqiNP_001035879.1, NM_001042414.2 [Q8WXF1-1]
XP_006719907.1, XM_006719844.3
XP_016876139.1, XM_017020650.1
UniGeneiHs.213198

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SDEX-ray1.90A61-320[»]
5IFNX-ray3.17A/B61-320[»]
5WPAX-ray2.29B61-320[»]
ProteinModelPortaliQ8WXF1
SMRiQ8WXF1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120558, 47 interactors
DIPiDIP-39028N
IntActiQ8WXF1, 27 interactors
MINTiQ8WXF1
STRINGi9606.ENSP00000343966

PTM databases

iPTMnetiQ8WXF1
PhosphoSitePlusiQ8WXF1
SwissPalmiQ8WXF1

Polymorphism and mutation databases

BioMutaiPSPC1
DMDMi74762636

Proteomic databases

EPDiQ8WXF1
MaxQBiQ8WXF1
PaxDbiQ8WXF1
PeptideAtlasiQ8WXF1
PRIDEiQ8WXF1
ProteomicsDBi75023
75024 [Q8WXF1-2]

Protocols and materials databases

DNASUi55269
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000338910; ENSP00000343966; ENSG00000121390 [Q8WXF1-1]
ENST00000471658; ENSP00000436038; ENSG00000121390 [Q8WXF1-2]
ENST00000492741; ENSP00000435921; ENSG00000121390 [Q8WXF1-2]
ENST00000619300; ENSP00000481916; ENSG00000121390 [Q8WXF1-1]
GeneIDi55269
KEGGihsa:55269
UCSCiuc021rgx.2 human [Q8WXF1-1]

Organism-specific databases

CTDi55269
DisGeNETi55269
EuPathDBiHostDB:ENSG00000121390.18
GeneCardsiPSPC1
H-InvDBiHIX0011177
HGNCiHGNC:20320 PSPC1
HPAiHPA038904
MIMi612408 gene
neXtProtiNX_Q8WXF1
OpenTargetsiENSG00000121390
PharmGKBiPA134968603
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0115 Eukaryota
ENOG410XQA0 LUCA
GeneTreeiENSGT00390000005004
HOGENOMiHOG000231095
HOVERGENiHBG009801
InParanoidiQ8WXF1
OMAiMDNREQE
OrthoDBiEOG091G09T7
PhylomeDBiQ8WXF1
TreeFamiTF315795

Enzyme and pathway databases

SIGNORiQ8WXF1

Miscellaneous databases

ChiTaRSiPSPC1 human
GenomeRNAii55269
PROiPR:Q8WXF1
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000121390 Expressed in 216 organ(s), highest expression level in testis
CleanExiHS_PSPC1
ExpressionAtlasiQ8WXF1 baseline and differential
GenevisibleiQ8WXF1 HS

Family and domain databases

CDDicd12586 RRM1_PSP1, 1 hit
cd12589 RRM2_PSP1, 1 hit
Gene3Di3.30.70.330, 2 hits
InterProiView protein in InterPro
IPR012975 NOPS
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR034522 PSP1_RRM1
IPR034523 PSP1_RRM2
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF08075 NOPS, 1 hit
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00360 RRM, 2 hits
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiPSPC1_HUMAN
AccessioniPrimary (citable) accession number: Q8WXF1
Secondary accession number(s): Q5JTQ3
, Q8NCZ9, Q8WXE8, Q9NV36
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: March 1, 2002
Last modified: November 7, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
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