UniProtKB - Q8WXE9 (STON2_HUMAN)
Stonin-2
STON2
Functioni
GO - Molecular functioni
- clathrin adaptor activity Source: GO_Central
GO - Biological processi
- hematopoietic progenitor cell differentiation Source: Ensembl
- membrane organization Source: Reactome
- regulation of endocytosis Source: UniProtKB
- synaptic vesicle endocytosis Source: UniProtKB
- vesicle-mediated transport Source: GO_Central
Keywordsi
Biological process | Endocytosis |
Enzyme and pathway databases
PathwayCommonsi | Q8WXE9 |
Reactomei | R-HSA-8856825, Cargo recognition for clathrin-mediated endocytosis R-HSA-8856828, Clathrin-mediated endocytosis |
SignaLinki | Q8WXE9 |
Names & Taxonomyi
Protein namesi | Recommended name: Stonin-2Alternative name(s): Stoned B |
Gene namesi | Name:STON2 Synonyms:STN2, STNB |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000140022.9 |
HGNCi | HGNC:30652, STON2 |
MIMi | 608467, gene |
neXtProti | NX_Q8WXE9 |
Subcellular locationi
Other locations
- Cytoplasm 1 Publication
- Membrane 1 Publication
- synaptosome By similarity
Note: Some fraction is membrane-associated.
Cytosol
- cytosol Source: HPA
Nucleus
- nucleolus Source: HPA
Other locations
- clathrin-coated vesicle Source: GO_Central
- cytoplasmic vesicle Source: GO_Central
- intracellular membrane-bounded organelle Source: GO_Central
- membrane Source: UniProtKB-SubCell
- neuron projection Source: UniProtKB-SubCell
- synaptic vesicle Source: GO_Central
Keywords - Cellular componenti
Cell junction, Cytoplasm, Membrane, Synapse, SynaptosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 738 | W → A: Reduces interaction with SYT1. 1 Publication | 1 | |
Mutagenesisi | 740 | K → A: Reduces interaction with SYT1. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 85439 |
OpenTargetsi | ENSG00000140022 |
PharmGKBi | PA143485624 |
Miscellaneous databases
Pharosi | Q8WXE9, Tbio |
Polymorphism and mutation databases
BioMutai | STON2 |
DMDMi | 34098614 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000185732 | 1 – 905 | Stonin-2Add BLAST | 905 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 255 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 281 | PhosphoserineCombined sources | 1 | |
Modified residuei | 287 | PhosphoserineCombined sources | 1 | |
Modified residuei | 302 | PhosphoserineCombined sources | 1 | |
Modified residuei | 762 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8WXE9 |
jPOSTi | Q8WXE9 |
MassIVEi | Q8WXE9 |
MaxQBi | Q8WXE9 |
PaxDbi | Q8WXE9 |
PeptideAtlasi | Q8WXE9 |
PRIDEi | Q8WXE9 |
ProteomicsDBi | 32734 75021 [Q8WXE9-1] |
PTM databases
CarbonylDBi | Q8WXE9 |
iPTMneti | Q8WXE9 |
PhosphoSitePlusi | Q8WXE9 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000140022, Expressed in kidney and 196 other tissues |
ExpressionAtlasi | Q8WXE9, baseline and differential |
Genevisiblei | Q8WXE9, HS |
Organism-specific databases
HPAi | ENSG00000140022, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with the second C2 domain of synaptotagmins SYT1 and SYT2.
Interacts with EPS15, EPS15R and ITSN1.
Interacts indirectly with the AP-2 adapter complex.
Interacts with TOR1A and COPS4; the interaction controls STON2 protein stability.
4 PublicationsBinary interactionsi
Hide detailsQ8WXE9
With | #Exp. | IntAct |
---|---|---|
EPS15 [P42566] | 17 | EBI-539742,EBI-396684 |
NEDD8 [Q15843] | 2 | EBI-539742,EBI-716247 |
Syt1 [P21707] from Rattus norvegicus. | 2 | EBI-539742,EBI-458098 |
GO - Molecular functioni
- clathrin adaptor activity Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 124526, 58 interactors |
CORUMi | Q8WXE9 |
ELMi | Q8WXE9 |
IntActi | Q8WXE9, 46 interactors |
MINTi | Q8WXE9 |
STRINGi | 9606.ENSP00000450857 |
Miscellaneous databases
RNActi | Q8WXE9, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q8WXE9 |
SMRi | Q8WXE9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8WXE9 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 427 – 560 | SHDPROSITE-ProRule annotationAdd BLAST | 134 | |
Domaini | 568 – 878 | MHDPROSITE-ProRule annotationAdd BLAST | 311 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 313 – 315 | NPF 1 | 3 | |
Motifi | 329 – 331 | NPF 2 | 3 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG2677, Eukaryota |
GeneTreei | ENSGT00940000159392 |
HOGENOMi | CLU_016541_0_0_1 |
InParanoidi | Q8WXE9 |
OMAi | WIELDDH |
OrthoDBi | 1059322at2759 |
PhylomeDBi | Q8WXE9 |
TreeFami | TF300393 |
Family and domain databases
DisProti | DP01368 |
InterProi | View protein in InterPro IPR036168, AP2_Mu_C_sf IPR028565, MHD IPR012320, SHD_dom IPR031228, STON2 IPR017110, Stonin IPR022699, Stonin2_N |
PANTHERi | PTHR10529:SF345, PTHR10529:SF345, 1 hit |
Pfami | View protein in Pfam PF00928, Adap_comp_sub, 1 hit PF12016, Stonin2_N, 1 hit |
PIRSFi | PIRSF037099, Stonin, 1 hit |
SUPFAMi | SSF49447, SSF49447, 1 hit |
PROSITEi | View protein in PROSITE PS51072, MHD, 1 hit PS51070, SHD, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTTLDHVIAT HQSEWVSFNE EPPFPAHSQG GTEEHLPGLS SSPDQSESSS
60 70 80 90 100
GENHVVDGGS QDHSHSEQDD SSEKMGLISE AASPPGSPEQ PPPDLASAIS
110 120 130 140 150
NWVQFEDDTP WASTSPPHQE TAETALPLTM PCWTCPSFDS LGRCPLTSES
160 170 180 190 200
SWTTHSEDTS SPSFGCSYTD LQLINAEEQT SGQASGADST DNSSSLQEDE
210 220 230 240 250
EVEMEAISWQ ASSPAMNGHP APPVTSARFP SWVTFDDNEV SCPLPPVTSP
260 270 280 290 300
LKPNTPPSAS VIPDVPYNSM GSFKKRDRPK STLMNFSKVQ KLDISSLNRT
310 320 330 340 350
PSVTEASPWR ATNPFLNETL QDVQPSPINP FSAFFEEQER RSQNSSISST
360 370 380 390 400
TGKSQRDSLI VIYQDAISFD DSSKTQSHSD AVEKLKQLQI DDPDHFGSAT
410 420 430 440 450
LPDDDPVAWI ELDAHPPGSA RSQPRDGWPM MLRIPEKKNI MSSRHWGPIF
460 470 480 490 500
VKLTDTGYLQ LYYEQGLEKP FREFKLEICH EISEPRLQNY DENGRIHSLR
510 520 530 540 550
IDRVTYKEKK KYQPKPAVAH TAEREQVIKL GTTNYDDFLS FIHAVQDRLM
560 570 580 590 600
DLPVLSMDLS TVGLNYLEEE ITVDVRDEFS GIVSKGDNQI LQHHVLTRIH
610 620 630 640 650
ILSFLSGLAE CRLGLNDILV KGNEIVLRQD IMPTTTTKWI KLHECRFHGC
660 670 680 690 700
VDEDVFHNSR VILFNPLDAC RFELMRFRTV FAEKTLPFTL RTATSVNGAE
710 720 730 740 750
VEVQSWLRMS TGFSANRDPL TQVPCENVMI RYPVPSEWVK NFRRESVLGE
760 770 780 790 800
KSLKAKVNRG ASFGSTSVSG SEPVMRVTLG TAKYEHAFNS IVWRINRLPD
810 820 830 840 850
KNSASGHPHC FFCHLELGSD REVPSRFANH VNVEFSMPTT SASKASVRSI
860 870 880 890 900
SVEDKTDVRK WVNYSAHYSY QVALGSIWLM LPTPFVHPTT LPLLFLLAML
TMFAW
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A3B3IU55 | A0A3B3IU55_HUMAN | Stonin-2 | STON2 | 977 | Annotation score: | ||
G3V322 | G3V322_HUMAN | Stonin-2 | STON2 | 49 | Annotation score: | ||
H0YJ05 | H0YJ05_HUMAN | Stonin-2 | STON2 | 128 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Sequence conflicti | 121 – 122 | TA → NS in AAK76362 (PubMed:11454741).Curated | 2 | ||
Isoform 2 (identifier: Q8WXE9-3) | |||||
Sequence conflicti | 899 | V → L in BAD92185 (Ref. 3) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_020182 | 307 | S → P3 PublicationsCorresponds to variant dbSNP:rs3813535Ensembl. | 1 | |
Natural variantiVAR_046643 | 646 | R → H. Corresponds to variant dbSNP:rs34323725Ensembl. | 1 | |
Natural variantiVAR_046644 | 694 | T → A. Corresponds to variant dbSNP:rs35689202Ensembl. | 1 | |
Natural variantiVAR_021912 | 851 | S → A. Corresponds to variant dbSNP:rs2241621Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_044863 | 872 – 905 | VALGS…TMFAW → TTETDNLPNPLYCSCLPHTD LKRGSKRVVKIRWNASLEVP LASSIRTMV in isoform 2. 1 PublicationAdd BLAST | 34 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF380833 mRNA Translation: AAK76362.1 Frameshift. AF449430 mRNA Translation: AAL47008.1 AB208948 mRNA Translation: BAD92185.1 Different initiation. AL121769 Genomic DNA No translation available. AL136040 Genomic DNA No translation available. BC069389 mRNA Translation: AAH69389.1 BC117493 mRNA Translation: AAI17494.1 AF255309 mRNA Translation: AAK57558.1 Sequence problems. |
CCDSi | CCDS58332.1 [Q8WXE9-3] |
RefSeqi | NP_001243359.1, NM_001256430.1 [Q8WXE9-3] NP_149095.2, NM_033104.3 |
Genome annotation databases
Ensembli | ENST00000555447; ENSP00000450857; ENSG00000140022 [Q8WXE9-3] ENST00000614646; ENSP00000477736; ENSG00000140022 [Q8WXE9-3] |
GeneIDi | 85439 |
KEGGi | hsa:85439 |
UCSCi | uc001xvk.3, human [Q8WXE9-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF380833 mRNA Translation: AAK76362.1 Frameshift. AF449430 mRNA Translation: AAL47008.1 AB208948 mRNA Translation: BAD92185.1 Different initiation. AL121769 Genomic DNA No translation available. AL136040 Genomic DNA No translation available. BC069389 mRNA Translation: AAH69389.1 BC117493 mRNA Translation: AAI17494.1 AF255309 mRNA Translation: AAK57558.1 Sequence problems. |
CCDSi | CCDS58332.1 [Q8WXE9-3] |
RefSeqi | NP_001243359.1, NM_001256430.1 [Q8WXE9-3] NP_149095.2, NM_033104.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2JXC | NMR | - | B | 301-340 | [»] | |
BMRBi | Q8WXE9 | |||||
SMRi | Q8WXE9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 124526, 58 interactors |
CORUMi | Q8WXE9 |
ELMi | Q8WXE9 |
IntActi | Q8WXE9, 46 interactors |
MINTi | Q8WXE9 |
STRINGi | 9606.ENSP00000450857 |
PTM databases
CarbonylDBi | Q8WXE9 |
iPTMneti | Q8WXE9 |
PhosphoSitePlusi | Q8WXE9 |
Polymorphism and mutation databases
BioMutai | STON2 |
DMDMi | 34098614 |
Proteomic databases
EPDi | Q8WXE9 |
jPOSTi | Q8WXE9 |
MassIVEi | Q8WXE9 |
MaxQBi | Q8WXE9 |
PaxDbi | Q8WXE9 |
PeptideAtlasi | Q8WXE9 |
PRIDEi | Q8WXE9 |
ProteomicsDBi | 32734 75021 [Q8WXE9-1] |
Protocols and materials databases
ABCDi | Q8WXE9, 1 sequenced antibody |
Antibodypediai | 164, 22 antibodies |
DNASUi | 85439 |
Genome annotation databases
Ensembli | ENST00000555447; ENSP00000450857; ENSG00000140022 [Q8WXE9-3] ENST00000614646; ENSP00000477736; ENSG00000140022 [Q8WXE9-3] |
GeneIDi | 85439 |
KEGGi | hsa:85439 |
UCSCi | uc001xvk.3, human [Q8WXE9-1] |
Organism-specific databases
CTDi | 85439 |
DisGeNETi | 85439 |
EuPathDBi | HostDB:ENSG00000140022.9 |
GeneCardsi | STON2 |
HGNCi | HGNC:30652, STON2 |
HPAi | ENSG00000140022, Low tissue specificity |
MIMi | 608467, gene |
neXtProti | NX_Q8WXE9 |
OpenTargetsi | ENSG00000140022 |
PharmGKBi | PA143485624 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2677, Eukaryota |
GeneTreei | ENSGT00940000159392 |
HOGENOMi | CLU_016541_0_0_1 |
InParanoidi | Q8WXE9 |
OMAi | WIELDDH |
OrthoDBi | 1059322at2759 |
PhylomeDBi | Q8WXE9 |
TreeFami | TF300393 |
Enzyme and pathway databases
PathwayCommonsi | Q8WXE9 |
Reactomei | R-HSA-8856825, Cargo recognition for clathrin-mediated endocytosis R-HSA-8856828, Clathrin-mediated endocytosis |
SignaLinki | Q8WXE9 |
Miscellaneous databases
BioGRID-ORCSi | 85439, 6 hits in 848 CRISPR screens |
ChiTaRSi | STON2, human |
EvolutionaryTracei | Q8WXE9 |
GenomeRNAii | 85439 |
Pharosi | Q8WXE9, Tbio |
PROi | PR:Q8WXE9 |
RNActi | Q8WXE9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000140022, Expressed in kidney and 196 other tissues |
ExpressionAtlasi | Q8WXE9, baseline and differential |
Genevisiblei | Q8WXE9, HS |
Family and domain databases
DisProti | DP01368 |
InterProi | View protein in InterPro IPR036168, AP2_Mu_C_sf IPR028565, MHD IPR012320, SHD_dom IPR031228, STON2 IPR017110, Stonin IPR022699, Stonin2_N |
PANTHERi | PTHR10529:SF345, PTHR10529:SF345, 1 hit |
Pfami | View protein in Pfam PF00928, Adap_comp_sub, 1 hit PF12016, Stonin2_N, 1 hit |
PIRSFi | PIRSF037099, Stonin, 1 hit |
SUPFAMi | SSF49447, SSF49447, 1 hit |
PROSITEi | View protein in PROSITE PS51072, MHD, 1 hit PS51070, SHD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | STON2_HUMAN | |
Accessioni | Q8WXE9Primary (citable) accession number: Q8WXE9 Secondary accession number(s): G3V2T7 Q96RU6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 15, 2003 |
Last sequence update: | March 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations