UniProtKB - Q8WXB1 (MT21A_HUMAN)
Protein
Protein N-lysine methyltransferase METTL21A
Gene
METTL21A
Organism
Homo sapiens (Human)
Status
Functioni
Protein-lysine methyltransferase that selectively trimethylates residues in heat shock protein 70 (HSP70) family members. Contributes to the in vivo trimethylation of Lys residues in HSPA1 and HSPA8. In vitro methylates 'Lys-561' in HSPA1, 'Lys-564' in HSPA2, 'Lys-585' in HSPA5, 'Lys-563' in HSPA6 and 'Lys-561' in HSPA8.3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 47 | S-adenosyl-L-methionine; via amide nitrogen1 Publication | 1 | |
Binding sitei | 94 | S-adenosyl-L-methionine1 Publication | 1 | |
Binding sitei | 125 | S-adenosyl-L-methionine; via amide nitrogen1 Publication | 1 | |
Binding sitei | 143 | S-adenosyl-L-methionine; via carbonyl oxygen1 Publication | 1 |
GO - Molecular functioni
- ATPase binding Source: BHF-UCL
- heat shock protein binding Source: UniProtKB
- Hsp70 protein binding Source: BHF-UCL
- protein-lysine N-methyltransferase activity Source: UniProtKB
- protein methyltransferase activity Source: UniProtKB
GO - Biological processi
- peptidyl-lysine methylation Source: BHF-UCL
- protein methylation Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
PathwayCommonsi | Q8WXB1 |
Reactomei | R-HSA-8876725, Protein methylation |
Names & Taxonomyi
Protein namesi | Recommended name: Protein N-lysine methyltransferase METTL21A (EC:2.1.1.-)Alternative name(s): HSPA lysine methyltransferase HSPA-KMT Hepatocellular carcinoma-associated antigen 557b Methyltransferase-like protein 21A |
Gene namesi | Name:METTL21A Synonyms:FAM119A, HCA557B |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:30476, METTL21A |
MIMi | 615257, gene |
neXtProti | NX_Q8WXB1 |
VEuPathDBi | HostDB:ENSG00000144401.14 |
Subcellular locationi
Other locations
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: GO_Central
Nucleus
- nucleoplasm Source: Reactome
Other locations
- cytoplasm Source: GO_Central
- protein-containing complex Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 94 | D → A: Abolishes methyltransferase activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 151194 |
OpenTargetsi | ENSG00000144401 |
PharmGKBi | PA145008433 |
Miscellaneous databases
Pharosi | Q8WXB1, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3588741 |
Genetic variation databases
BioMutai | METTL21A |
DMDMi | 150382834 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000292033 | 1 – 218 | Protein N-lysine methyltransferase METTL21AAdd BLAST | 218 |
Proteomic databases
EPDi | Q8WXB1 |
jPOSTi | Q8WXB1 |
MassIVEi | Q8WXB1 |
MaxQBi | Q8WXB1 |
PaxDbi | Q8WXB1 |
PeptideAtlasi | Q8WXB1 |
PRIDEi | Q8WXB1 |
ProteomicsDBi | 75001 [Q8WXB1-1] 75002 [Q8WXB1-2] |
PTM databases
iPTMneti | Q8WXB1 |
PhosphoSitePlusi | Q8WXB1 |
Expressioni
Gene expression databases
Bgeei | ENSG00000144401, Expressed in oocyte and 201 other tissues |
ExpressionAtlasi | Q8WXB1, baseline and differential |
Genevisiblei | Q8WXB1, HS |
Organism-specific databases
HPAi | ENSG00000144401, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsQ8WXB1
GO - Molecular functioni
- ATPase binding Source: BHF-UCL
- heat shock protein binding Source: UniProtKB
- Hsp70 protein binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 127352, 31 interactors |
IntActi | Q8WXB1, 16 interactors |
MINTi | Q8WXB1 |
STRINGi | 9606.ENSP00000415115 |
Miscellaneous databases
RNActi | Q8WXB1, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8WXB1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 73 – 75 | S-adenosyl-L-methionine binding1 Publication | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2793, Eukaryota |
GeneTreei | ENSGT00940000157249 |
HOGENOMi | CLU_055721_4_2_1 |
InParanoidi | Q8WXB1 |
OrthoDBi | 1588190at2759 |
PhylomeDBi | Q8WXB1 |
TreeFami | TF313206 |
Family and domain databases
InterProi | View protein in InterPro IPR019410, Methyltransf_16 IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR14614, PTHR14614, 1 hit |
Pfami | View protein in Pfam PF10294, Methyltransf_16, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8WXB1-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MALVPYEETT EFGLQKFHKP LATFSFANHT IQIRQDWRHL GVAAVVWDAA
60 70 80 90 100
IVLSTYLEMG AVELRGRSAV ELGAGTGLVG IVAALLGAHV TITDRKVALE
110 120 130 140 150
FLKSNVQANL PPHIQTKTVV KELTWGQNLG SFSPGEFDLI LGADIIYLEE
160 170 180 190 200
TFTDLLQTLE HLCSNHSVIL LACRIRYERD NNFLAMLERQ FTVRKVHYDP
210
EKDVHIYEAQ KRNQKEDL
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JES0 | C9JES0_HUMAN | Protein N-lysine methyltransferase ... | METTL21A LOC151194, hCG_15462 | 127 | Annotation score: | ||
H7BXH9 | H7BXH9_HUMAN | Protein N-lysine methyltransferase ... | METTL21A | 236 | Annotation score: | ||
C9JSX1 | C9JSX1_HUMAN | Protein N-lysine methyltransferase ... | METTL21A | 52 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 33 | I → T in BAC04229 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_032935 | 192 | T → I3 PublicationsCorresponds to variant dbSNP:rs2551949Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_026379 | 88 – 90 | AHV → GGI in isoform 2. 1 Publication | 3 | |
Alternative sequenceiVSP_026380 | 91 – 218 | Missing in isoform 2. 1 PublicationAdd BLAST | 128 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF455817 mRNA Translation: AAL66295.1 AK093812 mRNA Translation: BAC04229.1 AC079767 Genomic DNA Translation: AAX93175.1 BC009462 mRNA Translation: AAH09462.1 BC033720 mRNA Translation: AAH33720.1 |
CCDSi | CCDS2376.1 [Q8WXB1-1] CCDS82562.1 [Q8WXB1-2] |
RefSeqi | NP_001120867.1, NM_001127395.3 [Q8WXB1-1] NP_001294950.1, NM_001308021.2 NP_001317059.1, NM_001330130.1 [Q8WXB1-1] NP_001317060.1, NM_001330131.1 [Q8WXB1-2] NP_001317062.1, NM_001330133.1 [Q8WXB1-2] NP_001317063.1, NM_001330134.1 [Q8WXB1-1] NP_001317064.1, NM_001330135.1 [Q8WXB1-2] NP_660323.3, NM_145280.6 [Q8WXB1-1] XP_005246396.1, XM_005246339.3 [Q8WXB1-1] XP_005246397.1, XM_005246340.3 [Q8WXB1-1] |
Genome annotation databases
Ensembli | ENST00000406927; ENSP00000385481; ENSG00000144401 [Q8WXB1-1] ENST00000411432; ENSP00000415115; ENSG00000144401 [Q8WXB1-1] ENST00000425132; ENSP00000400730; ENSG00000144401 [Q8WXB1-2] ENST00000426075; ENSP00000403317; ENSG00000144401 [Q8WXB1-1] ENST00000442521; ENSP00000392062; ENSG00000144401 [Q8WXB1-1] ENST00000448007; ENSP00000407622; ENSG00000144401 [Q8WXB1-1] ENST00000458426; ENSP00000389684; ENSG00000144401 [Q8WXB1-2] |
GeneIDi | 151194 |
KEGGi | hsa:151194 |
UCSCi | uc002vce.4, human [Q8WXB1-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF455817 mRNA Translation: AAL66295.1 AK093812 mRNA Translation: BAC04229.1 AC079767 Genomic DNA Translation: AAX93175.1 BC009462 mRNA Translation: AAH09462.1 BC033720 mRNA Translation: AAH33720.1 |
CCDSi | CCDS2376.1 [Q8WXB1-1] CCDS82562.1 [Q8WXB1-2] |
RefSeqi | NP_001120867.1, NM_001127395.3 [Q8WXB1-1] NP_001294950.1, NM_001308021.2 NP_001317059.1, NM_001330130.1 [Q8WXB1-1] NP_001317060.1, NM_001330131.1 [Q8WXB1-2] NP_001317062.1, NM_001330133.1 [Q8WXB1-2] NP_001317063.1, NM_001330134.1 [Q8WXB1-1] NP_001317064.1, NM_001330135.1 [Q8WXB1-2] NP_660323.3, NM_145280.6 [Q8WXB1-1] XP_005246396.1, XM_005246339.3 [Q8WXB1-1] XP_005246397.1, XM_005246340.3 [Q8WXB1-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4LEC | X-ray | 2.28 | A/B | 8-218 | [»] | |
SMRi | Q8WXB1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 127352, 31 interactors |
IntActi | Q8WXB1, 16 interactors |
MINTi | Q8WXB1 |
STRINGi | 9606.ENSP00000415115 |
Chemistry databases
ChEMBLi | CHEMBL3588741 |
PTM databases
iPTMneti | Q8WXB1 |
PhosphoSitePlusi | Q8WXB1 |
Genetic variation databases
BioMutai | METTL21A |
DMDMi | 150382834 |
Proteomic databases
EPDi | Q8WXB1 |
jPOSTi | Q8WXB1 |
MassIVEi | Q8WXB1 |
MaxQBi | Q8WXB1 |
PaxDbi | Q8WXB1 |
PeptideAtlasi | Q8WXB1 |
PRIDEi | Q8WXB1 |
ProteomicsDBi | 75001 [Q8WXB1-1] 75002 [Q8WXB1-2] |
Protocols and materials databases
Antibodypediai | 34188, 165 antibodies |
DNASUi | 151194 |
Genome annotation databases
Organism-specific databases
CTDi | 151194 |
DisGeNETi | 151194 |
GeneCardsi | METTL21A |
HGNCi | HGNC:30476, METTL21A |
HPAi | ENSG00000144401, Low tissue specificity |
MIMi | 615257, gene |
neXtProti | NX_Q8WXB1 |
OpenTargetsi | ENSG00000144401 |
PharmGKBi | PA145008433 |
VEuPathDBi | HostDB:ENSG00000144401.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2793, Eukaryota |
GeneTreei | ENSGT00940000157249 |
HOGENOMi | CLU_055721_4_2_1 |
InParanoidi | Q8WXB1 |
OrthoDBi | 1588190at2759 |
PhylomeDBi | Q8WXB1 |
TreeFami | TF313206 |
Enzyme and pathway databases
PathwayCommonsi | Q8WXB1 |
Reactomei | R-HSA-8876725, Protein methylation |
Miscellaneous databases
BioGRID-ORCSi | 151194, 4 hits in 876 CRISPR screens |
ChiTaRSi | METTL21A, human |
GenomeRNAii | 151194 |
Pharosi | Q8WXB1, Tbio |
PROi | PR:Q8WXB1 |
RNActi | Q8WXB1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000144401, Expressed in oocyte and 201 other tissues |
ExpressionAtlasi | Q8WXB1, baseline and differential |
Genevisiblei | Q8WXB1, HS |
Family and domain databases
InterProi | View protein in InterPro IPR019410, Methyltransf_16 IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR14614, PTHR14614, 1 hit |
Pfami | View protein in Pfam PF10294, Methyltransf_16, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MT21A_HUMAN | |
Accessioni | Q8WXB1Primary (citable) accession number: Q8WXB1 Secondary accession number(s): Q53RV0, Q8N1Z9, Q96GH6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 26, 2007 |
Last sequence update: | June 26, 2007 | |
Last modified: | February 10, 2021 | |
This is version 134 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants