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Protein

Negative elongation factor B

Gene

NELFB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. May be able to induce chromatin unfolding.4 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRepressor, RNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-9603505 NTRK3 as a dependence receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Negative elongation factor B
Short name:
NELF-B
Alternative name(s):
Cofactor of BRCA1
Gene namesi
Name:NELFB
Synonyms:COBRA1, KIAA1182
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000188986.5
HGNCiHGNC:24324 NELFB
MIMi611180 gene
neXtProtiNX_Q8WX92

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi25920
PharmGKBiPA162382703

Polymorphism and mutation databases

BioMutaiNELFB
DMDMi38372378

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191291 – 580Negative elongation factor BAdd BLAST580

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei519N6-acetyllysineCombined sources1
Modified residuei557PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8WX92
MaxQBiQ8WX92
PaxDbiQ8WX92
PeptideAtlasiQ8WX92
PRIDEiQ8WX92
ProteomicsDBi74978

PTM databases

iPTMnetiQ8WX92
PhosphoSitePlusiQ8WX92
SwissPalmiQ8WX92

Expressioni

Tissue specificityi

Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.1 Publication

Gene expression databases

BgeeiENSG00000188986 Expressed in 225 organ(s), highest expression level in anterior cingulate cortex
CleanExiHS_COBRA1
ExpressionAtlasiQ8WX92 baseline and differential
GenevisibleiQ8WX92 HS

Organism-specific databases

HPAiHPA020259

Interactioni

Subunit structurei

The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE; the N-terminus of NELFB binds to the NELFA:NELFCD subcomplex. Interacts with the first BRCT repeat of BRCA1. Interacts with KIAA1191.3 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi117421, 53 interactors
CORUMiQ8WX92
DIPiDIP-32670N
IntActiQ8WX92, 31 interactors
MINTiQ8WX92
STRINGi9606.ENSP00000339495

Structurei

3D structure databases

ProteinModelPortaliQ8WX92
SMRiQ8WX92
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NELF-B family.Curated

Phylogenomic databases

eggNOGiENOG410IEBF Eukaryota
ENOG410YK9P LUCA
HOGENOMiHOG000231489
HOVERGENiHBG052596
InParanoidiQ8WX92
KOiK15180
OrthoDBiEOG091G04WH
PhylomeDBiQ8WX92
TreeFamiTF324620

Family and domain databases

InterProiView protein in InterPro
IPR010405 COBRA1
PANTHERiPTHR13503 PTHR13503, 1 hit
PfamiView protein in Pfam
PF06209 COBRA1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q8WX92-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFAGLQDLGV ANGEDLKETL TNCTEPLKAI EQFQTENGVL LPSLQSALPF
60 70 80 90 100
LDLHGTPRLE FHQSVFDELR DKLLERVSAI ASEGKAEERY KKLEDLLEKS
110 120 130 140 150
FSLVKMPSLQ PVVMCVMKHL PKVPEKKLKL VMADKELYRA CAVEVKRQIW
160 170 180 190 200
QDNQALFGDE VSPLLKQYIL EKESALFSTE LSVLHNFFSP SPKTRRQGEV
210 220 230 240 250
VQRLTRMVGK NVKLYDMVLQ FLRTLFLRTR NVHYCTLRAE LLMSLHDLDV
260 270 280 290 300
GEICTVDPCH KFTWCLDACI RERFVDSKRA RELQGFLDGV KKGQEQVLGD
310 320 330 340 350
LSMILCDPFA INTLALSTVR HLQELVGQET LPRDSPDLLL LLRLLALGQG
360 370 380 390 400
AWDMIDSQVF KEPKMEVELI TRFLPMLMSF LVDDYTFNVD QKLPAEEKAP
410 420 430 440 450
VSYPNTLPES FTKFLQEQRM ACEVGLYYVL HITKQRNKNA LLRLLPGLVE
460 470 480 490 500
TFGDLAFGDI FLHLLTGNLA LLADEFALED FCSSLFDGFF LTASPRKENV
510 520 530 540 550
HRHALRLLIH LHPRVAPSKL EALQKALEPT GQSGEAVKEL YSQLGEKLEQ
560 570 580
LDHRKPSPAQ AAETPALELP LPSVPAPAPL
Length:580
Mass (Da):65,697
Last modified:March 1, 2002 - v1
Checksum:iC0A4F7F2BEE52CBD
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0X1KG71A0A0X1KG71_HUMAN
Negative elongation factor B
NELFB
628Annotation score:

Sequence cautioni

The sequence AAH11892 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB14157 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAB43381 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti156L → F in BAB14157 (PubMed:14702039).Curated1
Sequence conflicti182S → A (PubMed:11230166).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF464935 mRNA Translation: AAL69965.1
AK022651 mRNA Translation: BAB14157.1 Different initiation.
AK091056 mRNA Translation: BAG52272.1
BX255925 Genomic DNA No translation available.
BC011892 mRNA Translation: AAH11892.1 Different initiation.
AL050280 mRNA Translation: CAB43381.3 Different initiation.
AB033008 mRNA Translation: BAA86496.1
PIRiT08747
RefSeqiNP_056271.3, NM_015456.4
UniGeneiHs.521809

Genome annotation databases

EnsembliENST00000634710; ENSP00000489248; ENSG00000188986
GeneIDi25920
KEGGihsa:25920
UCSCiuc004cmm.5 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF464935 mRNA Translation: AAL69965.1
AK022651 mRNA Translation: BAB14157.1 Different initiation.
AK091056 mRNA Translation: BAG52272.1
BX255925 Genomic DNA No translation available.
BC011892 mRNA Translation: AAH11892.1 Different initiation.
AL050280 mRNA Translation: CAB43381.3 Different initiation.
AB033008 mRNA Translation: BAA86496.1
PIRiT08747
RefSeqiNP_056271.3, NM_015456.4
UniGeneiHs.521809

3D structure databases

ProteinModelPortaliQ8WX92
SMRiQ8WX92
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117421, 53 interactors
CORUMiQ8WX92
DIPiDIP-32670N
IntActiQ8WX92, 31 interactors
MINTiQ8WX92
STRINGi9606.ENSP00000339495

PTM databases

iPTMnetiQ8WX92
PhosphoSitePlusiQ8WX92
SwissPalmiQ8WX92

Polymorphism and mutation databases

BioMutaiNELFB
DMDMi38372378

Proteomic databases

EPDiQ8WX92
MaxQBiQ8WX92
PaxDbiQ8WX92
PeptideAtlasiQ8WX92
PRIDEiQ8WX92
ProteomicsDBi74978

Protocols and materials databases

DNASUi25920
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000634710; ENSP00000489248; ENSG00000188986
GeneIDi25920
KEGGihsa:25920
UCSCiuc004cmm.5 human

Organism-specific databases

CTDi25920
DisGeNETi25920
EuPathDBiHostDB:ENSG00000188986.5
GeneCardsiNELFB
HGNCiHGNC:24324 NELFB
HPAiHPA020259
MIMi611180 gene
neXtProtiNX_Q8WX92
PharmGKBiPA162382703
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IEBF Eukaryota
ENOG410YK9P LUCA
HOGENOMiHOG000231489
HOVERGENiHBG052596
InParanoidiQ8WX92
KOiK15180
OrthoDBiEOG091G04WH
PhylomeDBiQ8WX92
TreeFamiTF324620

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-9603505 NTRK3 as a dependence receptor

Miscellaneous databases

ChiTaRSiNELFB human
GeneWikiiCofactor_of_BRCA1
GenomeRNAii25920
PROiPR:Q8WX92
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000188986 Expressed in 225 organ(s), highest expression level in anterior cingulate cortex
CleanExiHS_COBRA1
ExpressionAtlasiQ8WX92 baseline and differential
GenevisibleiQ8WX92 HS

Family and domain databases

InterProiView protein in InterPro
IPR010405 COBRA1
PANTHERiPTHR13503 PTHR13503, 1 hit
PfamiView protein in Pfam
PF06209 COBRA1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNELFB_HUMAN
AccessioniPrimary (citable) accession number: Q8WX92
Secondary accession number(s): A2BFA3
, Q96EW5, Q9H9R4, Q9ULN8, Q9Y3W0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: March 1, 2002
Last modified: September 12, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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